12 research outputs found
A tĂĽnetmentes pikkelysömörös bĹ‘r patholĂłgiás elváltozásainak szerepe a betegsĂ©g fenotĂpusának megjelenĂ©sĂ©ben = The contribution of inherent abnormalities of non-lesional skin to disease phenotype in psoriasis
Az elvĂ©gzett kutatás alapkutatás, amely jelentĹ‘s rĂ©sze klinikai jellegű, a pikkelysömör bĹ‘rbetegsĂ©g pathomechanizmusának megĂ©rtĂ©sĂ©t cĂ©lozza, ezáltal terápiás cĂ©lpontokat jelöl ki a betegsĂ©g gyĂłgyĂtásában. Közvetlen hasznosĂtás az elmĂşlt 4 Ă©vben nem törtĂ©nt, de ez nem is volt kitűzött cĂ©l, azonban a betegsĂ©g kialakulásával kapcsolatban számos Ăşj adatot szolgáltattunk, melyeknek egy rĂ©sze szakmánk vezetĹ‘ folyĂłirataiban publikáciĂłra is kerĂĽlt. Az utĂłbbi Ă©v eredmĂ©nyei Ăşj távlatokat nyitottak további kutatásaink cĂ©lkitűzĂ©seihez. A pályázatban vázolt munkáktĂłl, csak annyiban tĂ©rtĂĽnk el, amennyiben bizonyos eredmĂ©nyek más terĂĽleten valĂł hasznosĂtása Ă©rtelemszerűen kĂvánkozott, valamint amennyiben kutatásunk eredmĂ©nyei Ăşj, fontosnak ĂtĂ©lhetĹ‘ megválaszolandĂł kĂ©rdĂ©seket tettek fel. A munka eddig megjelent dolgozatai mellett 2 cikk közvetlen benyĂşjtás elĹ‘tt, kettĹ‘ pedig az Ărás fázisában van. 1 hallgatĂł a pályázat ideje alatt ebbĹ‘l a munkábĂłl Ărt Ă©s szerzett PhD fokozatot, jelenleg 2 hallgatĂł PhD-ja kĂ©szĂĽl rĂ©szben az itt elvĂ©gzett munkákat is tartalmazva. | Within the project we carried out basic research toward the understanding of psoriasis pathomechanisms. Our research carries the possibility of new therapies in this common skin disease. We have made considerable progress in understanding key mechanisms of keratinocyte hyperproliferation, the major cutaneous characteristic of this skin disease. Our research work went according to the proposed plan, minor changes were made as required by experimental data. Some of the results have already been published in leading journals of our field, we are about to submit two papers and two is in the process of writing. One PhD student who worked on the project has successfully defended her thesis and two are presently working on their theses
Prealbumin epitópok haptén hordozó szerepének vizsgálata limfocita transzformációs tesztben a gyógyszerallergia igazolására = The role of prealbumin as a hapten-carrier protein in lymphocyte transformation test for the establishment of drug allergy
Korábbi vizsgálataink alapján cĂ©lul tűztĂĽk ki, hogy megvizsgáljuk a prealbumin egyes szerkezeti elemeinek haptĂ©n hordozĂł szerepĂ©t Ă©s kĂĽlönbözĹ‘ gyĂłgyszerekhez kapcsolva nagyszámĂş betegben meghatározzuk, növelhetĹ‘-e a limfocita transzformáciĂłs teszt (LTT) diagnosztikus hatĂ©konysága. 119 biztosan gyĂłgyszer Ă©rzĂ©kenysĂ©gben szenvedĹ‘ beteg csak gyĂłgyszerrel ill. gyĂłgyszer-prealbumin peptid konjugátummal vĂ©gzett párhuzamos LTT vizsgálata során nem találtunk konzekvens kĂĽlönbsĂ©get a gyĂłgyszer-peptid javára az LTT vizsgálat Ă©rzĂ©kenysĂ©gĂ©ben. GyĂłgyszeraktiválást követĹ‘en a kĂĽlönbözĹ‘ immunglobulinok (IgD, IgM, IgG) sejtfelszĂni kifejezĹ‘dĂ©se individuálisan változott a betegek B limfocitáin. 84 gĂ©n expressziĂłját vizsgáltuk 2 egĂ©szsĂ©ges egyĂ©n Ă©s 4 gyĂłgyszerallergiában szenvedĹ‘ beteg izolált limfocitáin a gyĂłgyszerindukciĂłt követĹ‘en, amely gĂ©nek a CD4+ helper T limfociták TH1-TH2 tĂpusaival voltak kapcsolatban. A betegek esetĂ©ben az Ă©rzĂ©kenyĂtĹ‘ gyĂłgyszer hatására sokkal több vizsgált gĂ©n kifejezĹ‘dĂ©sĂ©nek növekedĂ©se következett be. Az is megállapĂthatĂł volt, hogy a TH1 Ă©s TH2 tĂpusĂş válaszok egyaránt indukálĂłdnak a gyĂłgyszerallergiás betegeknĂ©l, bár egyĂ©nenkĂ©nt kĂĽlönbözĹ‘ kifejezĹ‘dĂ©si mintázatot mutattak. EredmĂ©nyeink megerĹ‘sĂtik azt a vĂ©lekedĂ©st, hogy a gyĂłgyszerallergia mind klinikai megjelenĂ©sĂ©ben Ă©s lefolyásában, mind immunolĂłgiai mechanizmusaiban heterogĂ©n kĂłrkĂ©p, a TH1 Ă©s TH2 tĂpusĂş válaszok keverednek. | Human prealbumin acting as a hapten-carrier protein in drug allergy was suggested by our previous investigations. The aim of the work was that different structural elements of prealbumin acting as hapten-carrier peptides, in conjugation of different drugs, are able to increase the diagnostic sensitivity of lymphocyte transformation test (LTT) in drug allergy patients comparing to simple drugs. Gene expressions of drug-specific lymphocytes during drug activation were also investigated. Unfortunately, drug-prealbumin conjugates as activators were not able to increase the diagnostic sensitivity of LTT test comparing to simple drug activation based on detailed parallel measurements in 119 patients with proved drug allergy. Immunoglobulin expressions (IgD, IgM, IgG) on the drug-induced B lymphocytes of the patients are individually varied compared to freshly isolated B lymphocytes of the patients as well as to healthy controls.We investigated the expressions of 84 genes (in connection with TH1 and/or TH2 immune response) in freshly separated and drug-induced lymphocytes isolated from healthy controls and patients with drug allergy. The expressions of a lot of genes are increased in the drug-induced lymphocytes of drug allergy patients, however, their expression patterns are varied individually. Our results supported that drug allergy is a heterogeneous disorder in its clinical picture and immunological mechanisms. Both TH1 and TH2 immune responses are involved in the pathogenesis
Hyperproliferation of normally Quiescent keratinocytes in non-lesional psoriatic skin due to high Calcium concentration
Calcium plays an important role in the regulation of different functions of keratinocytes. In the present work we studied the effect of different extracellular calcium concentrations (0.01 mM-2.0 mM) on the proliferation and differentiation of human keratinocytes in normal human and non-lesional psoriatic skin. Using explant culture model, the proliferative and differentiated subsets of keratinocytes were detected by specific antibodies related to cell proliferation [beta-1 integrin (CD29), proliferating cell antigen (Ki67), proliferating cell nuclear antigen (PCNA)] and differentiation [differentiated cell cytokeratins (K1/K10) and differentiating cell antigen (lectin Ulex europaius agglutinin, UEA-1)]. After 4 days of culturing at high Ca2+ (2.0 mM) we observed marked hyperproliferation among the normally quiescent keratinocytes of non-lesional psoriatic skin. In normal uncultured and cultured skin and in uncultured and two-day-cultured non-lesional psoriatic skin both at normal (1.2 mM) and at high (2.0 mM) Ca2+ concentration only one layer of basal CD29+/Ki67+/K1/K10-/UEA-1- cell was observed. In sections from non-lesional psoriatic skin cultured for 4 days in the presence of high Ca2+ (2.0 mM) this cell population has expanded from at least three layers above the basement membrane. This expanded cell population of the 4-day high Ca2+ cultured non-lesional skin showed clear PCNA positive staining on frozen sections with the strongest positivity among the most basal localized cells. These data suggest that extracellular Ca2+ concentration can influence the proliferation of basal ("stem") keratinocytes, the proliferative response to high Ca2+ concentration of psoriatic non-lesional basal keratinocytes differs from that of normal basal keratinocytes, changes in the extracellular Ca2+ milieu might play a role in the induction of the hyperproliferative psoriatic lesion
The interleukin-8 receptor - a potential target for antipsoriatic therapy
Högskolan i Borås har av Södra Älvsborgs Sjukhus fått i uppdrag att genomföra en utvärdering av den interna kursen Kompetensutveckling för undersköterskor, som pågått sedan 2002. Utvärderingen skall ligga till grund för fortsatt erbjudande av kursen, vilket innebär att såväl innehåll och tid som arbetsformen skall vara föremål för utvärderingen.Kursen Kompetensutveckling för undersköterskor är en frivillig intern utbildning, på Södra Älvsborgs Sjukhus i Borås, Skene och Alingsås, som vänder sig enbart till undersköterskor för att höja deras kompetens inom yrkeslivet. Kursen är ett Mål 3-projekt som kan kunna permanentas. En anledning till att göra utvärderingen är att många undersköterskor under de närmaste åren kommer att gå i pension och Södra Älvsborgs Sjukhus vill veta vilken beredskap de ska ha för att kunna erbjuda såväl nuvarande som presumtiva undersköterskor samma kompetensutvecklingsmöjligheter som hittills
Haloperidol attenuates beta-amyloid-induced calcium imbalance in human fibroblasts
Background: Antipsychotics are widely used in the treatment of behavioral and psychological symptoms of dementia. A low frequency of Alzheimer's disease in patients with schizophrenia is reported, and it has been proposed that antipsychotic medications, such as haloperidol, may be responsible. Disruption of intracellular calcium levels is considered to play a key role in beta-amyloid-induced neurotoxicity in Alzheimer's disease. Haloperidol has also been reported to interact with calcium homeostasis through dopamine-2 and sigma-1 receptors, and other, yet unknown mechanisms. Objective: Therefore, we investigated whether differences in the basal intracellular free calcium levels of cultured cutaneous fibroblasts - cells that do not express dopamine-2 and sigma-1 receptors - derived from sporadic Alzheimer patients and from age-matched control individuals after haloperidol treatment might be present. Methods: Intracellular calcium level was measured in Fura-2AM-loaded human fibroblasts by dual wavelength spectrofluorimetry. Results: Alzheimer cells exhibited significantly lower calcium level as compared to the control cultures. Exposure of fibroblasts to beta-amyloid peptide resulted in increased calcium concentration of the control cells, but not of Alzheimer fibroblasts. Co-incubation of cultures with a therapeutic dose of haloperidol blocked the beta-amyloid-induced elevation of calcium. Conclusion: This finding indicates that haloperidol efficiently countervails ionic imbalance and suggests that it may serve as a potential agent in alleviating neurotoxic effects of beta-amyloid peptide