Evolutionary cell biology: Functional insight from “Endless forms most beautiful”

In animal and fungal model organisms, the complexities of cell biology have been analyzed in exquisite detail and much is known about how these organisms function at the cellular level. However, the model organisms cell biologists generally use include only a tiny fraction of the true diversity of eukaryotic cellular forms. The divergent cellular processes observed in these more distant lineages are still largely unknown in the general scientific community. Despite the relative obscurity of these organisms, comparative studies of them across eukaryotic diversity have had profound implications for our understanding of fundamental cell biology in all species and have revealed the evolution and origins of previously observed cellular processes. In this Perspective, we will discuss the complexity of cell biology found across the eukaryotic tree, and three specific examples of where studies of divergent cell biology have altered our understanding of key functional aspects of mitochondria, plastids, and membrane trafficking

Principles For Aiding Complex Military Decision Making

Paper presented to the Second International Command and Control Research and Technology Symposium, Monterey, Ca.The Tactical Decision Making Under Stress (TADMUS) program is being conducted to apply recent developments in decision theory and human-system interaction technology to the design of a decision support system for enhancing tactical decision making under the highly complex conditions involved in anti-air warfare scenarios in littoral environments. Our goal is to present decision support information in a format that minimizes any mismatches between the cognitive characteristics of the human decision maker and the design and response characteristics of the decision support system. Decision makers are presented with decision support tools which parallel the cognitive strategies they already employ, thus reducing the number of decision making errors. Hence, prototype display development has been based on decision making models postulated by naturalistic decision-making theory. Incorporating current human-system interaction design principles is expected to reduce cognitive processing demands and thereby mitigate decision errors caused by cognitive overload, which have been documented through research and experimentation. Topics include a discussion of: (1) the theoretical background for the TADMUS program; (2) a description of the cognitive tasks performed; (3) the decision support and human- system interaction design principles incorporated to reduce the cognitive processing load on the decision maker; and (4) a brief description of the types of errors made by decision makers and interpretations of the cause of these errors based on the cognitive psychology literature.Funding for the research cited in this paper was received from the Cognitive and Neural Science and Technology Division of the Office of Naval Research

Influence of blade aerodynamic model on prediction of helicopter rotor aeroacoustic signatures

Brown’s vorticity transport model has been used to investigate how the local blade aerodynamic model influences the quality of the prediction of the high-frequency airloads associated with blade–vortex interactions, and thus the accuracy with which the acoustic signature of a helicopter rotor can be predicted. The vorticity transport model can accurately resolve the structure of the wake of the rotor and allows significant flexibility in the way that the blade loading can be represented. The Second Higher-Harmonic Control Aeroacoustics Rotor Test was initiated to provide experimental insight into the acoustic signature of a rotor in cases of strong blade–vortex interaction. Predictions of two models for the local blade aerodynamics are compared with the test data. A marked improvement in accuracy of the predicted high-frequency airloads and acoustic signature is obtained when a lifting-chord model for the blade aerodynamics is used instead of a lifting-line-type approach. Errors in the amplitude and phase of the acoustic peaks are reduced, and the quality of the prediction is affected to a lesser extent by the computational resolution of the wake, with the lifting-chord model producing the best representation of the distribution of sound pressure below the rotor

A Rawlsian basis for core labour rights

John Rawls’s Theory of Justice is the most influential work of political philosophy of recent times. Rawls does not, however, consider the issue of labour rights. This paper considers the applicability of the rights of workers to join unions, bargain collectively, and to strike under Rawls’s theory, in the context of empirical research showing how individuals are, in practice, best protected at work. We argue that, in developed countries at least, Rawls’s theory supports the core rights of workers to organize, strike and bargain collectively with employers, under a combination of Rawls’s first and second principles of justice. We then consider the international dimension, discussing how labour rights are to be viewed internationally both under Rawls’s own international theory in his Law of Peoples, and under globalist interpretations of his theory of justice

Regulation of protein kinase B and glycogen synthase kinase-3 by insulin and beta-adrenergic agonists in rat epididymal fat cells - Activation of protein kinase B by wortmannin-sensitive and -insensittve mechanisms

Previous studies using L6 myotubes have suggested that glycogen synthase kinase-3 (GSK-3) is phosphoryl ated and inactivated in response to insulin by protein kinase B (PKB, also known as Akt or RAG) (Cross, D, A, E., Alessi, D, R., Cohen, P., Andjelkovic, M., and Hemmings, B, A. (1995) Nature 378, 785-789), In the present study, marked increases in the activity of PKB have been shown to occur in insulin-treated rat epididymal fat cells with a time course compatible with the observed decrease in GSK-3 activity, Isoproterenol, acting primarily through beta(3)-adrenoreceptors, was found to decrease GSK-3 activity to a similar extent (approximately 50%) to insulin, However, unlike the effect of insulin, the inhibition of GSK by isoproterenol was not found to be sensitive to inhibition by the phosphatidylinositol 3'-kinase inhibitors, wortmannin or LY 294002, The change in GSK-3 activity brought about by isoproterenol could not be mimicked by the addition of permeant cyclic AMP analogues or forskolin to the cells, although at the concentrations used, these agents were able to stimulate lipolysis. Isoproterenol, but again not the cyclic AMP analogues, was found to increase the activity of PKB, although to a lesser extent than insulin. While wortmannin abolished the stimulation of PKB activity by insulin, it was without effect on the activation seen in response to isoproterenol, The activation of PKB by isoproterenol was not accompanied by any detectable change in the electrophoretic mobility of the protein on SDS-polyacrylamide gel electrophoresis. It would therefore appear that distinct mechanisms exist for the stimulation of PKB by insulin and isoproterenol in rat fat cells

EMG Analysis and Motor Unit Recruitment in the Rectus Femoris of College Students

The rectus femoris works as part of the quadriceps muscle group to provide the force needed to generate very explosive movements during knee extension. Because many sports require explosive movements from the lower body, many strength coaches implement exercise routines that strengthen the muscles and prevent injuries. In the general population, activities using knee extension are essential in getting the full benefit of a designed exercise program. Muscle fibers provide the contractile force needed to accomplish a movement. Commonly, a deficiency is present in people who tend to favor one leg more than the other during activity. This is due to factors such as improper training, improper biomechanics, and joint injury. Having a strength imbalance from one leg to the other can strongly influence the amount of force generated, and therefore the amount of weight lifted. The purpose of this study was to determine whether these subjects had an imbalance in the contractile force of the rectus femoris. The participants were all kinesiology students in a Master’s degree program. Their age ranges from 22 years of age to 25 years of age. The instruments being used for this procedure are a leg extension machine and Biopac EMG. Each participant was required to perform five reps of a single leg extension for each leg, after determining maximum voluntary contraction. To analyze data that is collected. Data was analyzed using SPSS and Microsoft Excel. Muscle contractions were compared between and within subjects and compared from the participant’s dominant to non-dominant leg. For this particular study, the purpose was calculating whether the contractions are different from dominant leg to non-dominant leg. The results indicate that participants have a significant decrease in the contractile force from their dominant to non-dominant leg; significance was set at p\u3c.05. This study shows where coaches, trainers, and athletes need to focus on strength training to correct muscle imbalances. Imbalances could indicate that subjects are not trained properly, or they are favoring one leg while performing explosive maneuvers, or have an injury that prevents proper firing motor unit recruitment (1, 4)

Energy Expenditure and Economy During a Cycle Ergometer Test

Research has shown that submaximal cycle ergometer testing presents a reduced V02 max score when compared to that of the treadmill test. In this study, we compared a submaximal economy test on the Monark Cycle Ergometer. The purpose of this study was for each participant to perform the same test and compare economy, V02 ml/kg/min for a given workload, as well as calories burned between males and females during the nine minute test. By testing economy for each participant, we aim to determine whether males or females use oxygen more efficiently during testing. Before beginning the test, the Cosmed metabolic equipment was calibrated. The test was standardized to last for nine minutes at 70 rotations per minute and 1 kilopond of resistance. Based off of the comparison in V02 mL/kg/min and kCal mL/kg/min, the women had the highest V02 per given workload and also had the lowest calories burned. The women were the least efficient by having a higher V02 while working at the same intensity as the male participants. The research used to support this study indicates that there are two components for overall metabolic power during a dynamic leg exercise on the cycle ergometer; the power used to overcome the resistance applied to the ergometer and the rate of energy expenditure required to move the lower limbs without opposition against the opposition load (Girardis, Linnarsson, Moia, Pendergast, & Ferretti, 1999). It is also stated that during exercise at submaximal levels below lactate threshold the net V02 of the whole body is equivalent of the net rate of energy expenditure (Storer, Davis, & Caiozzo, 1990). The cycle ergometer is a good use to determine V02 by collecting expired air and the volume measured in the spirometer (Saltin & Astrand, 1967). For the results of this lab to be more accurate the resistance and intensity could have been changed at different stages of the test. This would have given and more accurate measure of the ability of each participant to reach the next stage of testing

Constraints on the Progenitor of SN 2010jl and Pre-Existing Hot Dust in its Surrounding Medium

A search for the progenitor of SN~2010jl, an unusually luminous core-collapse supernova of Type~IIn, using pre-explosion {\it Hubble}/WFPC2 and {\it Spitzer}/IRAC images of the region, yielded upper limits on the UV and near-infrared (IR) fluxes from any candidate star. These upper limits constrain the luminosity and effective temperature of the progenitor, the mass of any preexisting dust in its surrounding circumstellar medium (CSM), and dust proximity to the star. A {\it lower} limit on the CSM dust mass is required to hide a luminous progenitor from detection by {\it Hubble}. {\it Upper} limits on the CSM dust mass and constraints on its proximity to the star are set by requiring that the absorbed and reradiated IR emission not exceed the IRAC upper limits. Using the combined extinction-IR emission constraints we present viable $M_d-R_1$ combinations, where $M_d$ and $R_1$ are the CSM dust mass and its inner radius. These depend on the CSM outer radius, dust composition and grain size, and the properties of the progenitor. The results constrain the pre-supernova evolution of the progenitor, and the nature and origin of the observed post-explosion IR emission from SN~2010jl. In particular, an $\eta$~Car-type progenitor will require at least 4~mag of visual extinction to avoid detection by the {\it Hubble}. This can be achieved with dust masses $\gtrsim 10^{-3}$~\msun\ (less than the estimated 0.2-0.5~\msun\ around $\eta$~Car) which must be located at distances of $\gtrsim 10^{16}$~cm from the star to avoid detection by {\it Spitzer}.Comment: Accepted for publication in the ApJ. 14 pages 10 figures. The complete figure set for Figure 10 (24 images) is available in the online journa

Exploring the use of a modified high-temperature, short-time continuous heat exchanger with extended holding time (HTST-EHT) for thermal inactivation of trypsin following selective enzymatic hydrolysis of the ß- lactoglobulin fraction in whey protein isolate

peer-reviewedTryptic hydrolysis of whey protein isolate under specific incubation conditions including a relatively high enzyme:substrate (E:S) ratio of 1:10 is known to preferentially hydrolyse ß-lactoglobulin (ß-LG), while retaining the other major whey protein fraction, i.e., x-lactalbumin(x-LA) mainly intact. An objective of the present work was to explore the e ects of reducing E:S (1:10 1:30, 1:50, 1:100) on the selective hydrolysis of ß-LG by trypsin at pH 8.5 and 25 °C in a 5% (w/v) WPI solution during incubation periods ranging from 1 to 7 h. In addition, the use of a pilot-scale continuous high-temperature, short-time (HTST) heat exchanger with an extended holding time (EHT) of 5 min as a means of inactivating trypsin to terminate hydrolysis was compared with laboratory-based acidification to <pH 3 by the addition of HCl, and batch sample heating in a water bath at 85 °C. An E:S of 1:10 resulted in 100% and 30% of ßLG and x-LA hydrolysis, respectively, after 3 h, while an E:S reduction to 1:30 and 1:50 led >90% ß-LG hydrolysis after respective incubation periods of 4 and 6 h, with <5% hydrolysis of x-LA in the case of 1:50. Continuous HTST-EHT treatment was shown to be an e ective inactivation process allowing for the maintenance of substrate selectivity. However, HTST-EHT heating resulted in protein aggregation, which negatively impacts the downstream recovery of intact -LA. An optimum E:S was determined to be 1:50, with an incubation time ranging from 3 h to 7 h leading to 90% ß-LG hydrolysis and minimal degradation of x-LA. Alternative batch heating by means of a water bath to inactivate trypsin caused considerable digestion of x-LA, while acidification to <pH 3.0 restricted subsequent functional applications of the protein