GIS in Indigenous Communities isn't Participatory GIS: Examples in Honduras and Mexico

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Monitoring and modeling primary production in coastal waters: studies in Massachusetts Bay 1992-1994

During 1992-1994, we made shipboard incubations suitable for determining rates of primary production in water from Boston Harbor, Massachusetts Bay, and Cape Cod Bay (Massachusetts, USA). These measurements were part of an extensive baseline monitoring program to characterize water quality prior to diversion of effluent from Boston Harbor directly into Massachusetts Bay via a submarine outfall diffuser. Production (P) was measured using whole-water samples exposed to irradiance (I) levels from ~5 to 2000 µE m-2 s-1. P-I incubations were performed on 6 surveys a year, spaced to capture principal features of the annual production cycle. The number of stations and depths examined varied between years. There were 10 stations and 2 depths sampled in 1992-1993. In 1994, we performed in-depth studies at 2 stations (Boston Harbor\u27s edge and western Massachusetts Bay) by sampling 4 depths. Using depth-intensive 1994 data a simple empirical regression model, using information on chlorophyll biomass, incident daily light, and the depth of the photic zone, predicted integrated primary production rates derived from P-I incubations. The regression model was virtually the same as described for other coastal waters, giving confidence in general use of the model as an extrapolation tool. Using the 1994-based empirical model, we obtained favorable comparisons with production rates modeled from 1992-1993 P-I incubations. Combining the regression model with data on chlorophyll, light, and the photic zone collected on frequent hydrographic surveys (up to 16 yr-1), annual primary production was estimated for 1992-1994. Primary production in an intensively studied region of western Massachusetts Bay (21 hydrographic profile stations in an area ~100 km2) ranged from 386 to 468 g C m-2 yr-1. For a station at the edge of Boston Harbor near Deer Island extrapolations suggested production rates of 263 to 546 g C m-2 yr-1. Based on 2 stations in central Cape Cod Bay (1992-1993 only), model extrapolations suggested an annual production of 527 to 613 g C m-2 yr-1. Analyses using incubation and modeling results suggested that production variability was strongly related to fluctuations in incident irradiance, especially at daily to seasonal time scales. Chlorophyll variability secondarily influenced production, especially at seasonal to annual time scales. Finally, we provide a case where equivalent production was achieved in environments with contrasting water quality (nutrient and chlorophyll concentrations) because of variations in the depth of the photic zone (controlled by both chlorophyll and non-chlorophyll turbidity). Comparative analyses showed that our study estimates of primary production were consistent with the literature on nutrient-rich shelf environments. In conclusion, our study validated an empirical modeling approach to determining primary production in coastal marine waters

The effects of the filter-feeding clam Mercenaria mercenaria on carbon cycling in experimental marine mesocosms

The metabolism and the fate of 14C labelled carbon was examined in 4 outdoor mesocosm (13 m3) tanks containing both benthic and pelagic compartments. Mesocosms with (16/m2) and without the clam, Mercenaria mercenaria were compared. System production, net and gross sedimentation of particulate carbon and benthic remineralization of dissolved inorganic nitrogen were all greater in mesocosms with clams. A filtration rate model, dependent on clam size and temperature, explained between 74–114% of the increased gross sedimentation in clam tanks relative to controls.The higher production in the clam tanks was at least in part due to a greater flux of dissolved inorganic nitrogen from the benthos. Despite this greater production in the clam tanks, water column biomass remained similar to controls. Calculations based on the filtration rate model indicated that clams could have consumed between 30% and 46% of the excess biomass produced during the day. Loss of particles due to processes in the water column appeared to consume most of this excess biomass. Although clams enhanced production and sedimentation, they did not limit phytoplankton biomass in the water column through filtration

Neurobiological effects of probiotic-supplemented diets in acutely stressed male Long-Evans rats: Evidence of enhanced resilience

Introduction ~Considering that the human intestine is home to almost 100 trillion microorganisms including bacteria (Cryan, 2011), recent research has focused on the role of the microbiome in neurobiological functions such as stress, anxiety and coping responses. ~Focusing on animal models, previous findings indicate that modifications of the gut microbiota via antibiotics and certain probiotics alter the anxiety response via the vagus nerve & immune system mediation (MacQueen et al., 2017). ~Recently, the concept of Psychobiotics has been introduced to refer to the use of microbiota to positively influence mental health outcomes (Foster et al., 2017)

Life-history diversity and productivity of Puget Sound Chinook salmon

Puget Sound Chinook are listed as threatened under the U.S. Endangered Species Act, and efforts to recover these populations are underway. Effective planning and implementation of conservation measures designed to promote recovery require knowledge of freshwater processes governing smolt production and juvenile rearing strategies. The Washington Department of Fish and Wildlife monitors these viability parameters through a network of smolt trapping sites throughout Puget Sound including the Nisqually River, Green River, Cedar River, Skagit River and Dungeness River. At these locations, we routinely observe two distinct pulses of subyearling migration: an early movement of small fry (\u3c 45 mm) captured immediately following emergence, and a later migration of larger parr (\u3e 45 mm) that rear and grow in freshwater for a variable period of time. Yearling Chinook are occasionally observed, but only at extremely low catches, and only in basins with cold, snow or glacial dominated headwaters. In terms of productivity, Ricker stock-recruit analysis provides evidence for density-dependence of parr but not fry abundance. This suggests limitation in the availability of rearing habitat for parr, and we hypothesize that fry migrants move downstream because they are unable to acquire rearing territories in habitats saturated with conspecifics. In watersheds where this result is observed, habitat restoration efforts that augment parr rearing habitat would have the greatest opportunity to benefit Chinook salmon productivity. We also find evidence for strong effects of stream flow on Chinook productivity, often as a negative relationship between peak flow and survival. Finally, our smolt trapping estimates permit analysis of smolt to adult return rates, which will also be presented

Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid

Background: Meiosis in higher vertebrates shows a dramatic sexual dimorphism: germ cells enter meiosis and arrest at prophase I during embryogenesis in females, whereas in males they enter mitotic arrest during embryogenesis and enter meiosis only after birth. Here we report the molecular analysis of meiosis onset in the chicken model and provide evidence for conserved regulation by retinoic acid

Connectivity and Nitrate Uptake Potential of Intermittent Streams in the Northeast USA

Non-perennial streams dominate the extent of stream networks worldwide. Intermittent streams can provide ecosystem services to the entire network—including nitrate uptake to alleviate eutrophication of coastal waters—and are threatened by lack of legal protection. We examined 12 intermittent streams in the temperate, humid climate of the Northeast USA. Over 3 years of monitoring, continuous flow was observed a median of 277 d yr−1, with no-flow conditions from early summer into fall. Estimated median discharge was 2.9 L s−1 or 0.36mm d−1. All intermittent streams originated from source wetlands (median area: 0.27 ha) and the median length of the intermittent stream from the source wetland to the downstream perennial stream was 344m. Through regional geospatial analysis with high resolution orthophotography, we estimated that widely available, “high resolution” (1:24,000) hydrography databases (e.g., NHDPlus HR) only displayed 43% of the total number of intermittent streams. Whole-stream gross nitrate-N uptake rates were estimated at six intermittent streams during continuous flow conditions using pulse additions of nitrate and a conservative tracer. These rates displayed high temporal variability (range: no detect to over 6,000mg N m−1 d−1); hot moments were noted in nine of the 65 pulse additions. Whole-stream gross nitrate-N uptake rates were significantly inversely related to discharge, with no measurable rates above 7 L s−1. Temperature was significantly positively correlated with whole-stream gross nitrate-N uptake rates, with more hot moments in the spring. Microbial assays demonstrated that nitrate cycling in intermittent streams are consistent with results from low order, perennial forested streams and highlighted the importance of debris dams and pools—potential locations for transient storage. Our assessment suggests that intermittent streams in our region may annually contribute 24–47% of the flow to perennial streams and potentially remove 4.1 to 80.4 kg nitrate-N km−2 annually. If development in these areas continues, perennial streams are in danger of losing a portion of their headwaters and potential nitrate uptake areas may become nitrate sources to downstream areas. These results argue to manage fluvial systems with a holistic approach that couples intermittent and perennial components

Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in mice

Oxidative stress, a central mediator of cardiovascular disease, results in loss of the prosthetic haem group of soluble guanylate cyclase (sGC), preventing its activation by nitric oxide (NO). Here we introduce Apo-sGC mice expressing haem-free sGC. Apo-sGC mice are viable and develop hypertension. The haemodynamic effects of NO are abolished, but those of the sGC activator cinaciguat are enhanced in apo-sGC mice, suggesting that the effects of NO on smooth muscle relaxation, blood pressure regulation and inhibition of platelet aggregation require sGC activation by NO. Tumour necrosis factor (TNF)-induced hypotension and mortality are preserved in apo-sGC mice, indicating that pathways other than sGC signalling mediate the cardiovascular collapse in shock. Apo-sGC mice allow for differentiation between sGC-dependent and -independent NO effects and between haem-dependent and -independent sGC effects. Apo-sGC mice represent a unique experimental platform to study the in vivo consequences of sGC oxidation and the therapeutic potential of sGC activators

Comparing teacher roles in Denmark and England

This article reports the findings of a comparative study of teaching in Denmark and England; its broader aim is to help develop an approach for comparing pedagogy. Lesson observations and interviews identified the range of goals towards which teachers in each country worked and the actions these prompted. These were clustered using the lens of Bernstein’s pedagogic discourse (1990; 1996) to construct teacher roles which provided a view of pedagogy. Through this approach we have begun to identify variations in pedagogy across two countries. All teachers in this study adopted a variety of roles; of significance was the ease with which competent English teachers moved between roles. The English teachers observed adopted roles consistent with a wider techno-rationalist discourse. There was a greater subject emphasis by Danish teachers whose work was set predominantly within a democratic humanist discourse, whilst the English teachers placed a greater emphasis on applied skills