On the local dynamics of polynomial difference equations with fading stochastic perturbations

We examine the stability-instability behaviour of a polynomial difference equa- tion with state-independent, asymptotically fading stochastic perturbations. We find that the set of initial values can be partitioned into a stability region, an instability region, and a region of unknown dynamics that is in some sense \small". In the ¯rst two cases, the dynamic holds with probability at least 1 ¡ °, a value corresponding to the statistical notion of a confidence level. Aspects of an equation with state-dependent perturbations are also treated. When the perturbations are Gaussian, the difference equation is the Euler-Maruyama dis- cretisation of an It^o-type stochastic differential equation with solutions displaying global a.s. asymptotic stability. The behaviour of any particular solution of the difference equation can be made consistent with the corresponding solution of the differential equation, with probability 1 ¡ °, by choosing the stepsize parameter sufficiently small. We present examples illustrating the relationship between h, ° and the size of the stability region

Comparison of 3D scanned human models for off-body communications using motion capture

Body area networks are complex to analyze as there are several channel mechanisms occurring simultaneously, i.e. environmental multipath together with body motion and close coupling between worn antennas and human tissue. Electromagnetic (EM) simulation is an important tool since not all studies can be done on a real human. In order to gain insight into off-body communication involving a worn antenna, this paper uses a 3D animated model obtained from a 3D surface scanner and a motion capture system for full wave simulation of channels at 2.45 and 5.5GHz. To evaluate if the model can represent body area radio channels in general, a comparison of S21 of the simulated model with measurements from 5 other models of similar height to the main test subject is presented

Passive wireless tags for tongue controlled assistive technology interfaces

Tongue control with low profile, passive mouth tags is demonstrated as a human–device interface by communicating values of tongue-tag separation over a wireless link. Confusion matrices are provided to demonstrate user accuracy in targeting by tongue position. Accuracy is found to increase dramatically after short training sequences with errors falling close to 1% in magnitude with zero missed targets. The rate at which users are able to learn accurate targeting with high accuracy indicates that this is an intuitive device to operate. The significance of the work is that innovative very unobtrusive, wireless tags can be used to provide intuitive human–computer interfaces based on low cost and disposable mouth mounted technology. With the development of an appropriate reading system, control of assistive devices such as computer mice or wheelchairs could be possible for tetraplegics and others who retain fine motor control capability of their tongues. The tags contain no battery and are intended to fit directly on the hard palate, detecting tongue position in the mouth with no need for tongue piercings

Comparative advantages of small-scale forestry among emerging forest tenures

Forestland tenure institutions and patterns are in a period of rapid change in the USA. Historically dominant forestland tenures are disappearing, and new tenures are emerging. Traditional, vertically-integrated forest products firms have shed their forestland holdings to be picked up by Timber Investment Management Organizations (TIMOs) and Real Estate Investment Trusts (REITs), increasing numbers of private individuals and families are purchasing small rural tracts, and some communities are developing innovative means to gain control over nearby forestlands in order to protect these lands from commercial real estate development. Within this context of rapid ownership change, small-scale forest owners including families and communities find themselves at a competitive disadvantage, relative to large corporate owners, in wood commodity markets. This paper considers how small-scale forest tenures, relative to large corporate tenures, may be advantageous to society with regard to selected ecological, social, and economic factors. While the paper primarily draws upon illustrations from the United States Pacific Northwest, its themes are global in nature

pTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi

BACKGROUND: Trypanosoma cruzi is a protozoan pathogen of major medical importance in Latin America. It is also an early diverging eukaryote that displays many unusual biochemical features. The completion of the T. cruzi genome project has highlighted the need to extend the range of techniques available to study gene function. To this end we report the development of a stable tetracycline-dependent expression vector applicable to this parasite and describe in detail the parameters of the system. RESULTS: We first produced T. cruzi cell lines that constitutively expressed bacteriophage T7 RNA polymerase and the tetracycline repressor protein from a multicopy episome. An integrative vector with an inducible expression site under the control of a tetracycline-regulatable T7 promoter (pTcINDEX) was targeted to the transcriptionally silent ribosomal RNA spacer region of these parasites and transformants selected using a T7 RNA polymerase-dependent hygromycin resistance gene. To test the system we used two marker proteins, luciferase and red fluorescent protein (RFP), and an endogenous parasite protein (a mitochondrial superoxide dismutase). In each case we found that induction was both time and dose-dependent. Luciferase mRNA could be induced by at least 100-fold, and luciferase activity up to 60-fold, within 24 hours of the addition of tetracycline. When we examined RFP induction by confocal microscopy and fluorescence activated cell sorter, we observed very high levels of expression (>1000-fold increase in fluorescence intensity), although this was not synchronous throughout clonal populations. Induction of superoxide dismutase resulted in an 18-fold increase in cellular activity. The observation that a tagged version of the enzyme was correctly targeted to the mitochondrion demonstrates that our expression system may also provide a high-throughput strategy for subcellular localisation. CONCLUSION: Our results show that pTcINDEX represents a valuable addition to the genetic tools available for T. cruzi. The vector system is sufficiently flexible that it should have widespread uses including inducible expression of tagged proteins, generation of conditional knockout cell lines and the application of dominant-negative approaches

NASA Experience with CMM and CMMI

This slide presentation reviews the experience NASA has had in using Capability Maturity Model (CMM) and Capability Maturity Model Integration (CMMI). In particular this presentation reviews the agency's experience within the software engineering discipline and the lessons learned and key impacts from using CMMI

DNA methylation of ESR-1 and N-33 in colorectal mucosa of patients with Ulcerative Colitis (UC)

Introduction: Epigenetic marking such as DNA methylation influence gene transcription and chromosomal stability and may also be affected by environmental exposures. Few studies exist on alteration in DNA methylation profiles (genomic and gene specific methylation) in patients with Ulcerative Colitis (UC) and none assessing its relationship with lifestyle exposures. Aims & Methods: To assess genomic methylation and promoter methylation of the ESR-1 (oestrogen receptor - 1) and N-33 (tumour suppressor candidate-3) genes in the macroscopically normal mucosa of UC patients as well as to investigate effects of anthropometric and lifestyle exposures on DNA methylation. Sixty eight subjects were recruited (24 UC and 44 age and sex matched controls). Colorectal mucosal biopsies were obtained and DNA was extracted. Genomic DNA methylation was quantified using the tritium-labelled cytosine extension assay (3[H] dCTP) whilst gene specific methylation was quantified using the COBRA method. Results: The methylation level of both ESR-1 and N-33 genes were significantly higher in UC subjects compared with controls (7.9% vs 5.9%; p = 0.015 and 66% vs 9.3%; p < 0.001 respectively). There was no detectable difference in global DNA methylation between patients with UC and age and sex matched controls. No associations between indices of DNA methylation and anthropometric measures or smoking patterns were detected. Conclusions: For the first time, we have shown increased methylation in the promoter regions of the putative tumour suppressor gene N-33 in macroscopically normal mucosa of patients with UC. In addition, we have confirmed that methylation of ESR-1 promoter is higher in UC patients compared with age and sex matched controls. These findings suggests that, inactivation through methylation of the putative tumour suppressor genes N-33 and ESR-1, may not be associated with colorectal carcinogenesis in UC