A population survey and foraging analysis of the Double-crested cormorant (Phalacrocorax auritus) on the Santee lakes, South Carolina

This study was designed to determine whether Double-crested cormorants (Phalacrocorax auritus) were having an impact on the Santee lakes fishery for Striped bass (Morone saxatilis). I surveyed the lakes\u27 population in the summer of 2007 and winter of 2008 to determine population levels and 76 birds were necropsied in order to determine foraging preferences. Summer populations were estimated at 203 birds and winter at 6000. Clupeids (primarily Gizzard, Threadfin, and American shads) made up the bulk of the diet in both seasons with an overall percentage of 86.19%. No bass of any size or species were found in the stomachs of birds collected. Though this most likely indicates there is no direct impact by the Double-crested cormorants on the Striped bass fishery; however indirect effects are more difficult to quantify and more data is required before any conclusions can be drawn about effects from interspecific competition. The Double-crested cormorants do share a prey base with the Striped bass, and further examination of the energetic needs of the Striped bass as well as population estimates for the forage fish are necessary. Bomb calorimetry yielded the energetic densities for primary target fish and I estimated daily fish consumption for Double-crested cormorants on the Santee lakes to be approximately 8 fish/day. Management recommendations consist of continued population monitoring on lakes and roost harassment if local stakeholders continue to take issue with the birds\u27 presence

The Multiple Meanings of Global Health Governance: A Call for Conceptual Clarity

Background The term global health governance (GHG) is now widely used, with over one thousand works published in the scholarly literature, almost all since 2002. Amid this rapid growth there is considerable variation in how the term is defined and applied, generating confusion as to the boundaries of the subject, the perceived problems in practice, and the goals to be achieved through institutional reform. Methodology This paper is based on the results of a separate scoping study of peer reviewed GHG research from 1990 onwards which undertook keyword searches of public health and social science databases. Additional works, notably books, book chapters and scholarly articles, not currently indexed, were identified through Web of Science citation searches. After removing duplicates, book reviews, commentaries and editorials, we reviewed the remaining 250 scholarly works in terms of how the concept of GHG is applied. More specifically, we identify what is claimed as constituting GHG, how it is problematised, the institutional features of GHG, and what forms and functions are deemed ideal. Results After examining the broader notion of global governance and increasingly ubiquitous term “global health”, the paper identifies three ontological variations in GHG scholarship - the scope of institutional arrangements, strengths and weaknesses of existing institutions, and the ideal form and function of GHG. This has produced three common, yet distinct, meanings of GHG that have emerged – globalisation and health governance, global governance and health, and governance for global health. Conclusions There is a need to clarify ontological and definitional distinctions in GHG scholarship and practice, and be critically reflexive of their normative underpinnings. This will enable greater precision in describing existing institutional arrangements, as well as serve as a prerequisite for a fuller debate about the desired nature of GHG

Identifying Strategies and Metrics for Measuring Success and Long-Term Impact in Downtown Lewiston\u27s Tree Streets Neighborhood

Our report provides a detailed explanation of our processes to determine metrics for measuring long-term and short-term success in Lewiston’s Maine, downtown Tree Street Neighborhood. The aim of our work was to assist The City of Lewiston and their efforts to secure the Housing and Urban Developments (HUD), Choice Neighborhood Implementation Grant. We worked closely with Lewiston’s Healthy Neighborhood Development Team, whose focus is targeted to the Neighborhood component of the Implementation Grant. Using the four strategies given to us by the Healthy Neighborhood Development Team, our work focused on establishing metrics to support these strategies. Our approach to developing metrics was heavily influenced by the Goals of Lewiston’s “Growing Our Tree Streets” Transformation Plan to represent the Tree Streets Neighborhood positively and accurately. We specifically foregrounded our approach to metrics in an asset-based mindset to align ourselves with the Healthy Neighborhoods Development Team and the Transformation Plan while remaining cognizant of HUD’s requirements for metrics. We identified 24 metrics that support the four strategies while also supporting the larger Goals from the Transformation Plan. Our established metrics indicate success across six Goals of the Transformation Plan and while dually supporting the four strategies. Of the 24 metrics, 14 specifically satisfied HUD requirements. All metrics are accompanied by information to support the location and collection of data

Exploring Literacy Coaches\u27 Self-Efficacy to Identify Their Professional Development Needs

The International Literacy Association highlighted three coaching models that a literacy coach might employ: coaching to conform, into practice, and for transformation. While numerous researchers have explored the roles and tasks of literacy coaching, there is little research on these coaching models. Studies have described the disconnect between what a coach should be doing and what they are actually doing, and the perceptions of administrators related to coaching roles and tasks. While these studies have illuminated issues in literacy coaching, few have looked at the self-efficacy of literacy coaches to enact roles and tasks, and we believe none have explored the self-efficacy of coaches to employ coaching models. The article describe the development and initial testing of the Elementary Literacy Coaching Self-Efficacy (ELCSE) survey which established the validity and reliability of the survey. Potential uses of the ELCSE survey are described which include assisting teacher preparation programs, districts, and schools to identify the professional development needs of future and current literacy coaches, no matter what coaching model they employ

Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype

Abstract Background Recent studies have indicated the presence of multipotent mesenchymal stromal cells (MSCs) in human lung diseases. Excess airway smooth muscle, myofibroblasts and activated fibroblasts have each been noted in asthma, suggesting that mesenchymal progenitor cells play a role in asthma pathogenesis. We therefore sought to determine whether MSCs are present in the lungs of ovalbumin (OVA)-sensitized and challenged mice, a model of allergic airways disease. Methods Balb/c mice were sensitized and challenged with PBS or OVA over a 25 day period. Flow cytometry as well as colony forming and differentiation potential were used to analyze the emergence of MSCs along with gene expression studies using immunochemical analyses, quantitative polymerase chain reaction (qPCR), and gene expression beadchips. Results A CD45-negative subset of cells expressed Stro-1, Sca-1, CD73 and CD105. Selection for these markers and negative selection against CD45 yielded a population of cells capable of adipogenic, osteogenic and chondrogenic differentiation. Lungs from OVA-treated mice demonstrated a greater average colony forming unit-fibroblast (CFU-F) than control mice. Sorted cells differed from unsorted lung adherent cells, exhibiting a pattern of gene expression nearly identical to bone marrow-derived sorted cells. Finally, cells isolated from the bronchoalveolar lavage of a human asthma patient showed identical patterns of cell surface markers and differentiation potential. Conclusions In summary, allergen sensitization and challenge is accompanied by an increase of MSCs resident in the lungs that may regulate inflammatory and fibrotic responses.http://deepblue.lib.umich.edu/bitstream/2027.42/78265/1/1465-9921-11-127.xmlhttp://deepblue.lib.umich.edu/bitstream/2027.42/78265/2/1465-9921-11-127.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/78265/3/1465-9921-11-127-S1.DOCPeer Reviewe

Have Academic Libraries Overcome the Gender Wage Gap? An Analysis of Gender Pay Inequality

This report draws upon two data sets to examine the gender wage gap among member institutions of the Association of Research Libraries (ARL). The first data set consists of 35 years of salary survey data collected by ARL and is used to provide trend data on the gender wage gap from 1980 to 2014 as well as present an in-depth look at the wage gap in 2014. After controlling for variables such as years of experience, position, and type of library in the 2014 ARL Salary Survey data, results revealed that women on average made approximately 2 percent less than their male counterparts in 2014. The second data set comes from a survey of ARL institutions conducted by the researchers in 2015 and is used to explore the influence of additional variables on the gender wage gap that were not found in the ARL Salary Survey data. Results from both data sets suggest a substantial difference between the gender wage gap in ARL institutions and the workforce as a whole

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

The art of producing recombinant proteins with complex post-translational modifications represents a major challenge for studies of structure and function. The rapid establishment and high recovery from transiently-transfected mammalian cell lines addresses this barrier and is an effective means of expressing proteins that are naturally channeled through the ER and Golgi-mediated secretory pathway. Here is one protocol for protein expression using the human HEK293F and HEK293S cell lines transfected with a mammalian expression vector designed for high protein yields. The applicability of this system is demonstrated using three representative glycoproteins that expressed with yields between 95-120 mg of purified protein recovered per liter of culture. These proteins are the human FcγRIIIa and the rat α2-6 sialyltransferase, ST6GalI, both expressed with an N-terminal GFP fusion, as well as the unmodified human immunoglobulin G1 Fc. This robust system utilizes a serum-free medium that is adaptable for expression of isotopically enriched proteins and carbohydrates for structural studies using mass spectrometry and nuclear magnetic resonance spectroscopy. Furthermore, the composition of the N-glycan can be tuned by adding a small molecule to prevent certain glycan modifications in a manner that does not reduce yield

The impact of xanthine oxidase (XO) on hemolytic diseases

Hemolytic diseases are associated with elevated levels of circulating free heme that can mediate endothelial dysfunction directly via redox reactions with biomolecules or indirectly by upregulating enzymatic sources of reactive species. A key enzymatic source of these reactive species is the purine catabolizing enzyme, xanthine oxidase(XO) as the oxidation of hypoxanthine to xanthine and subsequent oxidation of xanthine to uric acid generates superoxide (O2•-) and hydrogen peroxide (H2O2). While XO has been studied for over 120 years, much remains unknown regarding specific mechanistic roles for this enzyme in pathologic processes. This gap in knowledge stems from several interrelated issues including: 1) lethality of global XO deletion and the absence of tissue-specific XO knockout models have coalesced to relegate proof-of-principle experimentation to pharmacology; 2) XO is mobile and thus when upregulated locally can be secreted into the circulation and impact distal vascular beds by high-affinity association to the glycocalyx on the endothelium; and 3) endothelial-bound XO is significantly resistant (\u3e 50%) to inhibition by allopurinol, the principle compound used for XO inhibition in the clinic as well as the laboratory. While it is known that circulating XO is elevated in hemolytic diseases including sickle cell, malaria and sepsis, little is understood regarding its role in these pathologies. As such, the aim of this review is to define our current understanding regarding the effect of hemolysis (free heme) on circulating XO levels as well as the subsequent impact of XO-derived oxidants in hemolytic disease processes

Expanding the Practice of Newsmaking Criminology to Enlist Criminologists, Criminal Justicians, and Social Workers in Shaping Discussions of School Violence: A Review of School Shootings from 1992-2013

Newsmaking criminology argues that criminologists should interpret, influence, and even shape the direction of newsworthy information about crime and justice to the extent that they aggressively make their presence known by engaging the media. This article calls for an expansion in the practice of newsmaking criminology to also include criminal justicians, as well as social workers when it comes to the issue of school violence. Recently, a number of shootings have occurred on school campuses in rural, urban, and suburban America. When these shootings are reported, they quickly become politicized and generate widespread attention from parents, law enforcement, politicians, community activists, and policy-makers. However, what appears to be missing from the discussion is input from criminologists, criminal justicians, and social workers who could bring a wealth of knowledge and understanding to these atrocities. This study used a random sample of 129 items from over a twenty-one year period in a content analysis to categorize the spokespersons used by the mass media to discuss school violence and school shootings