Propagación de arboles de teca Tectona Grandis L. f. por miniestacas

Vegetative propagation in forests allows for producing homogeneous plantations with desirable phenotypic characteristics over a short period of time. The purpose of this study was to assess the propagation of teak trees by using minicutting. The study took place at Canalate – Córdoba, Colombia, in 13 to 17 year old commercial teak stands, where 30 cm cuttings from the lower third of the treetop with two to four sprouts were taken and subsequently planted in a sand and alluvium substrate. Once these cuttings reached a 2-to-5-cm length, they were cut and later were applied rooting treatments mixed with IBA (indole butyric acid) and PVP (polivinilpirrolidona) in the following doses: Control(00:00), C1(2000:4000), C2(2000:8000), C3(2000:16000), C4(3000:4000), C5(3000:8000), C6(3000:16000), C7(5000:4000) y C8(5000:16000) mg L.1. They were later planted in a peat, in a random design with four replications. Percentages of survival in a moist chamber (% SCH), rooting shade house (% ECS), and the number of roots were evaluated. Results showed highly significant differences between treatments for rates of survival in humid chamber, and rooting in shade house with regard to the control, being the treatments C4(3000:4000) and C8(5000:16000) more efficient with values over 55 and 40% respectively. Regarding the number of roots, the highest number was obtained in the C7(5000:4000) treatment with 2,5 roots per sprout. It can be concluded that minicutting is a potential technique for the propagation of Tectonagrandis Linn F trees with high commercial value.La propagación vegetativa en forestales permite producir plantaciones homogéneas con características fenotípicas deseables en corto tiempo. Este trabajo tuvo como objetivo evaluar la propagación de árboles de teca, usando la técnica de miniestacas. La investigación se realizó en el municipio de Canalete, Córdoba, Colombia, en rodales comerciales de teca de 13 a 17 años de edad, en los cuales se tomaron estacas del tercio inferior de la copa, de 30 cm de longitud con dos a cuatro brotes, las cuales se plantaron en un sustrato de arena y aluvión. Cuando los brotes o miniestacas alcanzaron una longitud de dos a cinco cm, se cortaron y se les aplicó tratamientos de enraizamiento, con ácido indolbutirico AIB y polivinilpirrolidona PVP en las siguientes dosis: Testigo(00:00), T1(2000:4000), T2(2000:8000), T3(2000:16000), T4(3000:4000), T5(3000:8000), T6(3000:16000), T7(5000:4000) y T8(5000:16000) mg L.1; posteriormente se plantaron en turba, en un diseño completamente al azar con cuatro repeticiones. Se evaluaron los porcentajes de supervivencia en cámara húmeda, enraizamiento en casa sombra y número de raíces. Los resultados mostraron diferencias altamente signifiativas entre los tratamientos para porcentajes de supervivencia en cámara húmeda y enraizamiento en casa sombra con respecto al testigo, siendo los tratamientos T4(3000:4000) y T8(5000:16000) más efiientes, con valores superiores al 55 y 40% respectivamente. El mayor número de raíces se obtuvo con el tratamiento T7(5000:4000), con 2,5 raíces por brote. Se concluye que la técnica de miniestaca es una metodología potencial en la propagación de árboles de teca de alto valor comercial

Micropropagación de tectona grandis l. f. a partir de meristemos preexistentes

Teak (Tectona grandis) is a tropical forest tree introduced from India and highly appreciated in Colombia due to rapid growth and wood quality. However, massive production of clonal planting material is still a limiting factor. In vitro propagation allows clonally propagate superior adult plants with higher multiplication rates, genetic stability and sanity. In the present research the effect of different BAP levels (0.0, 0.25, 0.5, 0.75 and 1.0 mg L-1), supplied on MS semisolid medium, on multiplication of explants with pre-existing meristem isolated from adult plants was evaluated. Treatments were distributed with a CRD and te crops maintained at 25ȗC with 12-hour photoperiod. Plant survival after transferring on sand or peat was also evaluated under shade house conditions. ANOVA showed that BAP supply increased shoot multiplication. The addition of auxin is necessary and sufficient for in vitro rooting of micropropagated shoots of teak, while its level increases in proportion to the dose of AIB. Plants had higher survival to 90% in both substratesLa teca (Tectona grandis) es una especie forestal tropical introducida de la India, que por sus cualidades de madera y rápido crecimiento ha sido muy sembrada en Colombia; sin embargo, los procesos de producción masiva de material clonal para siembra siguen siendo una limitante. La propagación in vitro permite propagar clonalmente árboles seleccionados por sus características fenotípicas y alto rendimiento de la madera, incrementando la tasa de multiplicación y manteniendo un alto nivel de sanidad y estabilidad genética. En el presente estudio se evaluó el efecto de diferentes niveles de bencilaminopurina (0.0, 0.25, 0.5, 0.75 y 1.0 mg L-1), en medio semisólido de MS, sobre la micropropagación de explantes con meristemos preexistentes provenientes de árboles adultos de teca. Los tratamientos fueron distribuidos utilizando un DCA, con 20 repeticiones por cada tratamiento y los cultivos mantenidos a 20˚C con 12 horas de luz. La supervivencia de las plantas micropropagadas trasplantadas en turba o arena fue determinada bajo condiciones de casa malla. El ANOVA mostró que la presencia de BAP en el medio incrementa el número de nuevos brotes. La adición de auxinas es necesaria y suficiente para el enraizamiento in vitro de tallos micropropagados de teca, a la vez que incrementa su nivel en forma proporcional a la dosis de AIB. Las plantas tuvieron porcentajes de supervivencia superiores al 90% en ambos sustratos

Vegetative propagation of Calycophyllum spruceanum (Benth) K. Schum., Cariniana estrellensis (Raddi) Kuntze and Tectona grandis Linn. f. by minicutting

O presente estudo objetivou avaliar o potencial da miniestaquia como método de propagação vegetativa para as espécies florestais pau mulato (Calycophyllum spruceanum (Benth) K. Schum.), jequitibá rosa (Cariniana estrellensis (Raddi) Kuntze) e teca (Tectona grandis Linn. f.), determinando a eficiência da miniestaquia na produção de mudas das referidas espécies quanto a produção e sobrevivência das minicepas nas sucessivas coletas; ao enraizamento das miniestacas provenientes das coletas sucessivas; ao efeito da aplicação de diferentes dosagens de reguladores de crescimento (AIB e ANA) no enraizamento e na qualidade das mudas e a variação nos níveis dos açúcares redutores durante o enraizamento de miniestacas de pau mulato. O jardim miniclonal das espécies estudadas foi formado a partir de minicepas obtidas por propagação sexuada (semente) o qual foi conduzido a pleno sol e recebendo adubações periódicas a cada 15 dias. As brotações foram colhidas em intervalos de 15 dias para a teca e pau mulato e 30 dias para o jequitibá rosa. A sobrevivência das minicepas foi alta para as três espécies avaliadas. A produção de miniestacas por minicepa foi alta para o pau mulato e para o jequitibá rosa, mas foi baixo e influenciado pela época do ano para a teca. Os resultados demonstraram não ser necessário o uso de reguladores de crescimento para a propagação vegetativa por miniestaquia de teca e pau mulato, uma vez que apresentam alta capacidade de enraizamento por esta técnica independentemente da presença dos reguladores de crescimento, já para o jequitibá rosa a melhor dosagem e regulador foi 2000 mg L-1 de ANA. Para o pau mulato nas concentrações de 1000 e 2000 mg L-1 proporcionaram maior comprimento radicular aos 60 dias, tanto para o AIB quanto para o ANA. Houve também uma antecipação no início do enraizamento, ocorrendo antes da testemunha. Na análise dos carboidratos realizadas em miniestacas de pau mulato, verificou-se uma redução na concentração dos açúcares redutores com o crescimento radicular, coincidindo com uma elevação na síntese de amido, durante o período de 30 a 45 dias após o estaqueamento. As concentrações tanto de açúcares redutores quanto de amido, foram positivamente correlacionadas com a aplicação de reguladores de crescimento. Para o jequitibá rosa, a aplicação de regulador de crescimento ANA foi superior ao AIB, aumentando o enraizamento, apresentando diferença significativa pelo teste de F (P<0,01). A miniestaquia para as espécies avaliadas no presente estudo é tecnicamente viável, tornando-se uma alternativa para a produção de mudas destas espécies.The present study objectified to evaluate the potential of the minicutting a method of vegetative propagation for the forest species Calycophyllum spruceanum (Benth) K. Schum., Cariniana estrellensis (Raddi) Kuntze and Tectona grandis Linn. f., determining the efficiency of the minicutting in the seedlings production of changes of the related species how much productive through production and the survival of ministumps in the successive collections; the root of the minicuttings from the successive collections; the effect of the application of different dosages of growth regulators (AIB and ANA) in the rooting and the quality of seedlings, to determine countent of the reducing sugars during the rooting of minicuttings of Calycophyllum spruceanum (Benth) K. Schum. The miniclonal garden of the studied species was formed from ministumps gotten by reproductive propagation (seed) which was leadin the full sun and receiving periodic fertilizations every 15 days. The sprouts had been harvested in intervals of 15 days for the Tectona grandis Linn. f. and Calycophyllum spruceanum (Benth) K. Schum. and 30 days for Cariniana estrellensis (Raddi) Kuntze. The survival of ministumps was high for the three evaluated species. The production of minicuttings by ministumps was high for the Calycophyllum spruceanum (Benth) K. Schum. and Cariniana estrellensis (Raddi) Kuntze, but it was lowed e influenced f. by the time of the year for the Tectona grandis Linn. f.. The results had demonstrated not to be necessary the use of growth regulators for the vegetative propagation of Tectona grandis Linn. f. and Calycophyllum spruceanum (Benth) K., once the species has high capacity of rooting independentely of the grouth regulators. For the Cariniana estrellensis (Raddi) Kuntze the best dosage and grouth regulator was 2000 mg L-1 de ANA. For the Calycophyllum spruceanum (Benth) K. Schum. in the concentrations of 1000 and 2000 mg L-1 had provided to greater root length at 60 days, either for the AIB or for ANA. It had also an anticipation in the beginning of the rooting. In the analysis of the carbohydrate carried through in minicuttings of Calycophyllum spruceanum (Benth) K. Schum., a reduction in the concentration of the reducing sugars with the root growth was verified, coinciding with a rise in the starch synthesis, during the period of 30 the 45 days. The concentrations of reducing sugars and starch has been positively correlated with the application of growth regulators. For Cariniana estrellensis (Raddi) Kuntze, the application of growth regulator ANA was superior to the AIB, increasing the rooting, presenting significant difference for the test of F (P<0,01). The minicutting for the species evaluated in the present study is technical viable, becoming an alternative for the production of seedlings for these species.Conselho Nacional de Desenvolvimento Científico e Tecnológic

Photoautotrophy and ozonization of bioreactors in clonal micropropagation of Eucalyptus spp.

Os avanços do melhoramento genético e da biotecnologia florestal estão acompanhados por mudanças e desafios na clonagem plantas. Durante muitos anos o uso da miniestaquia tem sido o método de clonagem mais utilizado pelo setor florestal. Porém, o uso do cultivo in vitro vem tornando-se, cada ano, mais importante. As dificuldades apresentadas pelo processo ex vitro de produção de mudas, aumenta a necessidade de protocolos e sistemas eficientes de propagação em laboratório. Com isso, muitos conceitos do cultivo in vitro convencional como meio de cultivo, tipo de recipiente, sistema de produção, heterotrofia estão mudando. Os estudos relacionados aos sistemas fotoautotróficos, jardins clonais in vitro e a automatização dos sistemas vem crescendo e sendo fundamental para o futuro da clonagem de plantas. Estes buscam tornar mais acessível e eficiente o processo de produção de plantas in vitro. Assim, objetiva-se nesse trabalho avaliar o potencial de sistemas fotoautotrófico, a seleção de materiais com maior competência a micropropagação e o uso da esterilização com ozônio na produção de Eucayptus spp. em biorreatores. Analisou-se o uso do meio semi-sólido e líquido, buscando entender o crescimento de clones de Eucalyptus spp. em sistemas de meio líquido como os utilizados em biorreatores. Foi verificado no estudo que o uso de meio líquido proporcionou maior incremento de massas fresca e seca dos explantes, taxa de multiplicação e tamanho da maior brotação. Além das diferenças constatadas no crescimento do material, observou-se respostas diferenciadas entre os clones em relação ao meio de cultivo utilizado (semi-sólido e líquido) para a maioria das características mensuradas. Para avaliação do crescimento de genótipos de Eucalyptus spp., em ambientes de trocas gasosas quanto a multiplicação e alongamento in vitro, testou-se dois ambientes e 10 clones por fase in vitro. O ambiente sem trocas gasosas foi mais favorável para a propagação in vitro dos genótipos testados. Além das diferenças observadas entre ambientes, constatou-se efeito do fator genético sobre as características, o que possibilita a seleção de clones para propagação sob as condições analisadas. Em relação às características avaliadas, os clones de E. benthamii apresentaram melhor desempenho em comparação com os demais clones estudados. Aliados a trocas gasosas, outros fatores como redução de sacarose é luz foram estudados visando estabelecer clones de Eucalyptus spp. em sistemas fotoautotróficos em biorreatores. Todas as qualidades e intensidades lumínicas pesquisadas proporcionaram bom desenvolvimento dos clones de E. benthamii e E. dunnii cultivados in vitro. O uso de LED branca promoveu valores de clorofila total e de carotenoide mais próximos aos encontrados ex vitro para os clones testados. O uso de sacarose, adicionado no meio de cultivo in vitro, foi importante para estimular a produção na multiplicação e alongamento, bem como aumentar as taxas de clorofila total e de carotenoides. Visando sistemas alternativos de esterilização dos biorreatores e meio de cultura, diferentes tempos e concentrações de ozônio (O3) foram utilizados. O O3 pode ser utilizado em substituição à autoclavagem, realizando a ozonização primeiro os biorreatores vazios, com a exposição a 50 mg L-¹ de O3 por 15 min em fluxo constante de 0,5 L min-¹ e, posteriormente, a ozonização do meio de cultura líquido por 90 min com concentração de 50 mg L-¹ de O3 e fluxo de 0,5 L min-¹. Novos estudos devem ser conduzidos buscando optimizar os resultados de multiplicação, alongamento, aclimatação ex vitro, assim como buscar protolocos e tecnologias que aliadas ao ozônio, viabilize econômica e operacionalmente, em larga escala, os biorreatores como estratégia para microjardins clonais.Advances in genetic improvement and forest biotechnology are lonked to changes and challenges in cloning. For many years the use of mini-cutting has been the method of cloning used by the forestry sector, but the use of in vitro cultivation has become more important each year. The challenges posed by the seedling ex vitro production increase the necessity for efficient protocols and propagation systems in laboratory. With this, many concepts of conventional in vitro culture such as culture medium, container type, production system, heterotrophy are changing. An ever increasing gowth of studies related to photoautotrophic systems applied to in vitro clonal gardens and systems automation has been seen, and becoming instrumental for the future of plant cloning strategies. The objective is to make the in vitro plant production process more accessible and efficient. The aim of this work was to evaluate the potential of photoautotrophic systems, the selection of materials with greater competence in micropropagation and the use of ozone (O3) sterilization on bioreactors-based production of Eucalyptus spp. Solid and liquid media were evaluated in genotypes of Eucalyptus spp., to better understand the growth of this species in liquid medium systems in bioreactors. The use of liquid medium resulted in a greater increase of fresh and dry mass of the explants, multiplication rate and size of the elongated shoots. In addition to the differences observed in the growth of the material, varying responses were observed among the clones regarding the culture medium used (solid and liquid) for most of the characteristics evaluated. Aiming at evaluating if the gas exchange environments favored in vitro growth, multiplication and elongation genotypes of Eucalyptus spp., two environments and 10 clones per phase were tested. In general, the absence of gas exchange was the most favorable environment for the in vitro propagation. In addition to the observed differences between environments, it was verified the effect of the genetic factor on the characteristics, which allows the selection of clones for propagation under the conditions analyzed. Eucalyptus benthamii clones reached better performance compared to the other clones studied. In addition to gas exchange, other factors such as reduction of sucrose and light were studied in order to establish clones of Eucalyptus spp.grown under photoautotrophic conditions in bioreactors. According to the results, Eucalyptus benthamii and E. dunnii clones had good development in all light qualities and intensities tested. The use of white LED promoted values of total chlorophyll and carotenoid closer to those found ex vitro. The use of sucrose in the in vitro culture medium was important to stimulate production in multiplication and elongation, as well as increase the rates of total chlorophyll and carotenoids. Aiming at alternative systems of sterilization of bioreactors and culture medium, different times and concentrations of ozone (O3) were tested. It has been found that, ozone can be used to replace autoclaving, first ozonization of empty bioreactors with exposure to 50 mg L-¹ of O3 for 15 minutes at a constant flow of 0.5 L min-¹ and subsequent to ozonization of liquid culture medium for 90 min with concentration of 50 mg L-¹ O3 and flow of 0.5 L-¹ min. Further studies should be conducted to optimize the results for multiplication, elongation, ex vitro acclimatization, as well as to search for protolocos and technologies that used with O3 sterelization, improve economically and operationally bioreactors as a strategy for mini-clonal hedge

Micropropagación de tectona grandis l. F. A partir de meristemos preexistentes

La teca (Tectona grandis) es una especie forestal tropical introducida de la India, que por sus cualidades de madera y rápido crecimiento ha sido muy sembrada en Colombia; sin embargo, los procesos de producción masiva de material clonal para siembra siguen siendo una limitante. La propagación in vitro permite propagar clonalmente árboles seleccionados por sus características fenotípicas y alto rendimiento de la madera, incrementando la tasa de multiplicación y manteniendo un alto nivel de sanidad y estabilidad genética. En el presente estudio se evaluó el efecto de diferentes niveles de bencilaminopurina (0.0, 0.25, 0.5, 0.75 y 1.0 mg L-1), en medio semisólido de MS, sobre la micropropagación de explantes con meristemos preexistentes provenientes de árboles adultos de teca. Los tratamientos fueron distribuidos utilizando un DCA, con 20 repeticiones por cada tratamiento y los cultivos mantenidos a 20˚C con 12 horas de luz. La supervivencia de las plantas micropropagadas trasplantadas en turba o arena fue determinada bajo condiciones de casa malla. El ANOVA mostró que la presencia de BAP en el medio incrementa el número de nuevos brotes. La adición de auxinas es necesaria y suficiente para el enraizamiento in vitro de tallos micropropagados de teca, a la vez que incrementa su nivel en forma proporcional a la dosis de AIB. Las plantas tuvieron porcentajes de supervivencia superiores al 90% en ambos sustratos

Tectona grandis L. f. micropropagation from preexisting meristems

La teca (Tectona grandis) es una especie forestal tropical introducida de la India, que por sus cualidades de madera y rápido crecimiento ha sido muy sembrada en Colombia; sin embargo, los procesos de producción masiva de material clonal para siembra siguen siendo una limitante. La propagación in vitro permite propagar clonalmente árboles seleccionados por sus características fenotípicas y alto rendimiento de la madera, incrementando la tasa de multiplicación y manteniendo un alto nivel de sanidad y estabilidad genética. En el presente estudio se evaluó el efecto de diferentes niveles de bencilaminopurina (0.0, 0.25, 0.5, 0.75 y 1.0 mg L-1), en medio semisólido de MS, sobre la micropropagación de explantes con meristemos preexistentes provenientes de árboles adultos de teca. Los tratamientos fueron distribuidos utilizando un DCA, con 20 repeticiones por cada tratamiento y los cultivos mantenidos a 20˚C con 12 horas de luz. La supervivencia de las plantas micropropagadas trasplantadas en turba o arena fue determinada bajo condiciones de casa malla. El ANOVA mostró que la presencia de BAP en el medio incrementa el número de nuevos brotes. La adición de auxinas es necesaria y suficiente para el enraizamiento in vitro de tallos micropropagados de teca, a la vez que incrementa su nivel en forma proporcional a la dosis de AIB. Las plantas tuvieron porcentajes de supervivencia superiores al 90% en ambos sustratos

EFFECTS OF EXPLANT TYPE, CULTURE MEDIA AND PICLORAM AND DICAMBA GROWTH REGULATORS ON INDUCTION AND PROLIFERATION OF SOMATIC EMBRYOS IN EUCALYPTUS GRANDIS X E. UROPHYLLA1

ABSTRACT The objective of the present study was to test the effects of explant type, auxin concentrations, culture media, and auxin concentrations on the induction and proliferation of somatic embryos of Eucalyptus grandis x E. urophylla. Seeds and cotyledons were used as explants and inoculated in culture media containing 1.13, 2.26, 3.39 and 4.52 µM dicamba or 4.14, 10.35, 20.71 and 31.06 µM picloram. Embryogenic calli induced in the picloram treatments were used as explants and inoculated in semisolid or liquid media containing 4.14, 10.35, 20.71 and 31.06 µM picloram and keeping the origin of the embryogenic callus (seeds or cotyledons) and the concentration of picloram in those who were in the induction phase. Statistical, descriptive and anatomical analyses were performed. Induction of somatic pro-embryos into the juvenile plant material of Eucalyptus grandis x E. urophylla was performed using seeds or cotyledons as the source of explants, with the addition of dicamba and picloram as growth regulators. The use of cotyledons as a source of explants and the concentration of 4.1 µM picloram added to the culture media resulted in a higher induction of somatic pro-embryos. Proliferation of secondary somatic embryos was achieved using liquid medium added with picloram

NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics

Xenarthrans—anteaters, sloths, and armadillos—have essential functions forecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosys-tem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts withdomestic dogs, these species have been threatened locally, regionally, or even across their fulldistribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths.Our data set includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae(3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data onDasypus pilo-sus(Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized,but new genetic studies have revealed that the group is represented by seven species. In thisdata paper, we compiled a total of 42,528 records of 31 species, represented by occurrence andquantitative data, totaling 24,847 unique georeferenced records. The geographic range is fromthe southern United States, Mexico, and Caribbean countries at the northern portion of theNeotropics, to the austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regardinganteaters,Myrmecophaga tridactylahas the most records (n=5,941), andCyclopessp. havethe fewest (n=240). The armadillo species with the most data isDasypus novemcinctus(n=11,588), and the fewest data are recorded forCalyptophractus retusus(n=33). Withregard to sloth species,Bradypus variegatushas the most records (n=962), andBradypus pyg-maeushas the fewest (n=12). Our main objective with Neotropical Xenarthrans is to makeoccurrence and quantitative data available to facilitate more ecological research, particularly ifwe integrate the xenarthran data with other data sets of Neotropical Series that will become available very soon (i.e., Neotropical Carnivores, Neotropical Invasive Mammals, andNeotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure,habitat loss, fragmentation effects, species invasion, and climate change effects will be possiblewith the Neotropical Xenarthrans data set. Please cite this data paper when using its data inpublications. We also request that researchers and teachers inform us of how they are usingthese data

NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics

Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data