Gender accommodative versus transformative approaches: a comparative assessment within a post-harvest fish loss reduction intervention

Technical and social constraints limit value chain actors from equitably engaging in and benefiting from capture fisheries in low-income settings. Extension and development programs often focus on the former, which reflects a technocratic orientation of the fisheries sector and uncertainty about effective ways for development programs to engage with gender and other social constraints. This study presents empirical insights that address these challenges to fisheries development. The study took place in fishing camps in the Barotse Floodplain, Zambia to compare two approaches addressing gender constraints within a broader post-harvest fish loss reduction intervention: an accommodative and a transformative approach. The former embodied a more common ‘practical needs’ set of strategies to ensure female participation, while the latter comprised a communication tool embedded in an action research process to build critical consciousness. Results indicate that the use of a transformative approach led to significant changes in gender equal attitudes and women’s empowerment outcomes compared to only using an accommodative approach. Development programs working in fisheries can apply the findings to engage effectively with gender constraints, especially using transformative approaches to help enable women and men to overcome the social and technical barriers that constrain their lives and livelihoods

Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly

5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether 5S rRNA autonomy is critical for ribosome function and cell survival. By linking circularly permutated 5S rRNA with 23S rRNA we generated a bacterial strain devoid of free 5S rRNA. Viability of the engineered cells demonstrates that autonomous 5S rRNA is dispensable for cell growth under standard conditions and is unlikely to have essential functions outside the ribosome. The fully assembled ribosomes carrying 23S-5S rRNA are highly active in translation. However, the engineered cells accumulate aberrant 50S subunits unable to form stable 70S ribosomes. Cryo-EM analysis revealed a malformed peptidyl transferase center in the misassembled 50S subunits. Our results argue that the autonomy of 5S rRNA is preserved due to its role in ribosome biogenesis

Postharvest fish losses and unequal gender relations: drivers of the social-ecological trap in the Barotse Floodplain fishery, Zambia

The Barotse Floodplain fishery is an important source of livelihood for economically poor women and men in western Zambia. Current efforts by the Department of Fisheries and the traditional authority to manage the fishery can be characterized as weak. The use of unsustainable fishing practices and overfishing are pervasive. Drawing on resilience thinking, we examine the extent to which the existing fishery-dependent livelihood context represents a social-ecological trap, i.e., a process in which rigid and persistent behavioral responses are applied because of a lack of capacity to adapt, leading to overdependence on the fishery, and ultimately, unsustainable outcomes. We use a gender lens and look beyond the primary sector (fishing) to include considerations of a secondary sector (postharvest fish processing) as an important dimension in the social-ecological trap paradigm. We present findings from an empirical research project that tested improved fish processing technologies and a communication for social change innovation as an alternate way to view a possible escape pathway from the trap not commonly recognized in the literature. The results suggest that there is value in adapting the social-ecological trap thinking to include postharvest fish losses and unequal gender relations as drivers of the trap in the floodplain fishery, and that approaches that combine technical innovation to reduce losses with social innovation to improve gender relations may hold promise for enabling fishery-dependent people to shift pathways out of social-ecological traps

Retapamulin-assisted ribosome profiling reveals the alternative bacterial proteome

The use of alternative translation initiation sites enables production of more than one protein from a single gene, thereby expanding the cellular proteome. Although several such examples have been serendipitously found in bacteria, genome-wide mapping of alternative translation start sites has been unattainable. We found that the antibiotic retapamulin specifically arrests initiating ribosomes at start codons of the genes. Retapamulin-enhanced Ribo-seq analysis (Ribo-RET) not only allowed mapping of conventional initiation sites at the beginning of the genes, but strikingly, it also revealed putative internal start sites in a number of Escherichia coli genes. Experiments demonstrated that the internal start codons can be recognized by the ribosomes and direct translation initiation in vitro and in vivo. Proteins, whose synthesis is initiated at internal in-frame and out-of-frame start sites, can be functionally important and contribute to the “alternative” bacterial proteome. The internal start sites may also play regulatory roles in gene expression

Transcriptome and epigenome landscape of human cortical development modeled in organoids

Genes implicated in neuropsychiatric disorders are active in human fetal brain, yet difficult to study in a longitudinal fashion. We demonstrate that organoids from human pluripotent cells model cerebral cortical development on the molecular level before 16 weeks postconception. A multiomics analysis revealed differentially active genes and enhancers, with the greatest changes occurring at the transition from stem cells to progenitors. Networks of converging gene and enhancer modules were assembled into six and four global patterns of expression and activity across time. A pattern with progressive down-regulation was enriched with human-gained enhancers, suggesting their importance in early human brain development. A few convergent gene and enhancer modules were enriched in autism-associated genes and genomic variants in autistic children. The organoid model helps identify functional elements that may drive disease onset