Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004

Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. This study determined S. Enteritidis lineages before and after this pandemic event in Japan using multilocus sequence typing (MLST). Thirty S. Enteritidis strains were collected in Japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovine strain, a liquid egg strain and an eggshell strain. Strains showed nine phage types and 17 pulsed-field profiles with pulsed-field gel electrophoresis. All strains had homologous type 11 sequences without any nucleotide differences in seven housekeeping genes. These MLST results suggest that S. Enteritidis with the diversities revealed by phage typing and pulsed-field profiling has a highly clonal population. Although type 11 S. Enteritidis may exhibit both pleiotropic surface structure and pulsed-field type variation, it is likely to be a stable lineage derived from an ancestor before the 1980s and/or 1990s pandemic in Japan

The Effect of Thermal Treatment on Conversion to Silica of Silica / Polymethylmethacrylate Hybrid Films Prepared by Sol-Gel Method Using Perhydropolysilazane

Silica/polymethylmethacrylate (PMMA) hybrid thin films were successfully prepared on polybutyleneterephtalate (PBT) substrate by dip-coating using perhydropolysilazane (PHPS) as a silica source. The effect of thermal treatment on conversion from PHPS to Silica was investigated by SEM and FT-IR in detail. Ultraviolet radiation in conversion to silica of thin film was most effective compared with heating in electric furnace. Mechanical properties of silica and silica/PMMA hybrid thin films also were examined by vickers hardness and pencil scratch hardness

Isolation and characteristics of Shiga toxin 2f-producing Escherichia coli among pigeons in Kyushu, Japan.

An increasing number of Shiga toxin 2f-producing Escherichia coli (STEC2f) infections in humans are being reported in Europe, and pigeons have been suggested as a reservoir for the pathogen. In Japan, there is very little information regarding carriage of STEC2f by pigeons, prompting the need for further investigation. We collected 549 samples of pigeon droppings from 14 locations in Kyushu, Japan, to isolate STEC2f and to investigate characteristics of the isolates. Shiga toxin stx 2f gene fragments were detected by PCR in 16 (2.9%) of the 549 dropping samples across four of the 14 locations. We obtained 23 STEC2f-isolates from seven of the original samples and from three pigeon dropping samples collected in an additional sampling experiment (from a total of seven locations across both sampling periods). Genotypic and phenotypic characteristics were then examined for selected isolates from each of 10 samples with pulsed-field gel electrophoresis profiles. Eight of the stx 2f gene fragments sequenced in this study were homologous to others that were identified in Europe. Some isolates also contained virulence-related genes, including lpfA O26, irp 2, and fyuA, and all of the 10 selected isolates maintained the eae, astA, and cdt genes. Moreover, five of the 10 selected isolates contained sfpA, a gene that is restricted to Shiga toxin-producing E. coli O165:H2 and sorbitol-fermenting Shiga toxin-producing E. coli O157:NM. We document serotypes O152:HNM, O128:HNM, and O145:H34 as STEC2f, which agrees with previous studies on pigeons and humans. Interestingly, O119:H21 was newly described as STEC2f. O145:H34, with sequence type 722, was described in a German study in humans and was also isolated in the current study. These results revealed that Japanese zoonotic STEC2f strains harboring several virulence-related factors may be of the same clonal complexes as some European strains. These findings provide useful information for public health-related disease management strategies in Japan

The 2018 Hokkaido Eastern Iburi earthquake (M-JMA=6.7) was triggered by a strike-slip faulting in a stepover segment: insights from the aftershock distribution and the focal mechanism solution of the main shock

The Hokkaido Eastern Iburi earthquake (MJMA = 6.7) occurred on September 6, 2018, in the Hokkaido corner region where the Kurile and northeastern Japan island arcs meet. We relocated aftershocks of this intraplate earthquake immediately after the main shock by using data from a permanent local seismic network and found that aftershock depths were concentrated from 20 to 40 km, which is extraordinarily deep compared with other shallow intraplate earthquakes in the inland area of Honshu and Kyushu, Japan. Further, we found that the aftershock area consists of three segments. The first segment is located in the northern part of the aftershock area, the second segment lies in the southern part, and the third segment forms a stepover between the other two segments. The hypocenter of the main shock, from which the rupture initiated, is located on the stepover segment. The centroid moment tensor solution for the main shock indicates a reverse faulting, whereas the focal mechanism solution determined by using the first-motion polarity of the P wave indicates strike-slip faulting. To explain this discrepancy qualitatively, we present a model in which the rupture started as a small strike-slip fault in the stepover segment of the aftershock area, followed by two large reverse faulting ruptures in the northern and southern segments