Assessment of consumers’ preference to the use of garlic and cinnamon oils on stored beef and camel meat ‘Kilishi’

The effects of garlic, cinnamon and combination of garlic and cinnamon oils on storage quality of beef and camel meat Kilishi were determined. Meat from the  Longissimus dorsi of the hind limb of camel and cattle were used for the experiment. Kilishi produced from each species was grouped into four parts of 2kg each and treated with garlic, cinnamon, combination of garlic and cinnamon oils (1:1) and control (Kilishi without treatment). The products were packed in a brown paper and stored at room temperature from May to September, 2012. Organoleptic  assessment showed that there was no significant difference in the consumer preference for the products. However, fresh Kilishi were more acceptable at the beginning of storage in May than at the end of storage in September 2012. It was concluded that garlic and cinnamon oils had no effect on the overall acceptability of Kilishi. It was  recommended that garlic and cinnamon oils should be used to extend the shelf life of Kilishi during storage.Keywords: Meat, Kilishi, Garlic Oil, Cinnamon Oil, Longissimus dorsi, Nigeria, Organolepti

Morphological and biochemical characterization of strains of Xanthomonas axonopodis pv. vignicola isolated from cowpea grown in three agroecological zones in Nigeria

Cowpea bacterial blight (Xanthomonas axonopodis pv vignicola) is a devastating disease of cowpea in Nigeria. Accurate identification is the first step for an effective management option. Thus, X. axonopodis pv. vignicola isolated from cowpea (Vigna unguinculata (L.) Walp) grown in different Agro-ecological zones in Nigeria were characterized using morphological characteristics, biochemical and antibiotic sensitivity tests. Pot experiments were conducted on two susceptible lines of cowpea (IT90k – 76) and (IT84s – 2246 – 4) in the screen house. In vivo infectivity studies were carried out to determine the effect of each of theX. axonopodis pv. vignicola isolate groups on the cowpea. All isolates from each of the agroecological zones were classified into Group 1 (light yellow) and Group 2 (brownish yellow) based on their morphological presentation on nutrient agar medium. Results from four of the biochemical test showed differences between the two morphologically different groups. Group 2 isolates showed a large zone of inhibition to a Lincomycin hydrochloride while isolates from Group 1 were not affected by the action of the same antibiotic. Blight symptom was only observed when the two types of isolates were combined and inoculated simultaneously. This study showed that there are two morpho-types of X. axonopodis pv. vignicola and their synergetic infectivity results in blight symptoms expression in cowpea

Occurrence and population distribution of plant parasitic nematodes associated with roselle (Hibiscus sabdariffa L.) in northern Nigeria

Pests including nematodes have been reported as a limiting factor to the production of roselle worldwide. A survey of rosellefarmsinsix Northern Sates of Nigeria was conductedin 2013 to identify the diverse nematode species associated with the soils and roots of the plant and determine their population densities on farmers’ fields. Eightsoil and ten plant samples were randomly collected from each of twenty farms from which nematodes were extracted and identified. Four genera of plantparasitic nematodes (Meloidogyne spp., Helicotylenchus spp., Rotylenchulus spp.and Tylenchus spp.) were found in association with roots and rhizosphere of roselle plants. Meloidogyne were most populous (62.29%) on the roots while Tylenchuswas least (1.68%). Helicotylenchus spp appeared in highest density (50.05%) in plant rhizosphere, but not identified from the roots. The highest percentage of nematode population (22.7 %) wasfrom Katsina, followed by Sokoto (21.6%), Jigawa (17.4%), Zamfara (16.5%), Kano (15.1%) and Kaduna states (6.7%).The generally low nematode density observed might be due to low precipitation and high soil temperature of the six states. Occurrence of varied species of nematodes demands effective control measures for improved roselle productivity. Kano, Zamfara and Jigawawith low nematode population densities can be considered for further research towards the establishment of pest-free areas or area of low pest prevalence for roselle nematodes in Nigeria, useful in the agricultural export trade. Further studies are required on the determination of density economic threshold and the definitive roles of nematodes in causing low productivity of roselle.Keywords: Export value, Hibiscus sabdariffa, nematodes, population densities, pest-free are

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Evaluation of different detection assays for use in Leifsonia xyli subsp. xyli assessment programs

Ratoon stunting disease (RSD) caused by Leifsonia xyli subsp. xyli is a major constraint to sugarcane production worldwide. Importation of infected germplasm exposes Nigeria to risk of introduction of the pathogen since it has no unique external and internal symptoms. Therefore, different detection techniques were investigated. L. xyli subsp. xyli inoculated and uninoculated sugarcane varieties comprising susceptible (CP72-1210), moderately resistant (CP80-1743), most commonly grown (Co957, Co997, SP71-6180, Co62175, chewing cane, B47419, Co6806) and locally bred (NCS001, NCS002, NCS003, NCS005, NCS006, NCS007, NCS008, ILS001 and ILS002) were evaluated in the screenhouse for both plant crop and ratoon crop. RSD detection assays of PCR on leaf and stalk saps, evaporative-blot enzyme immunoassay (EB-EIA) on sap from stalk and tissue-blot enzyme immunoassay (TB-EIA) on stalk were carried out using inoculated and uninoculated varieties. L. xyli subsp. xyli was not detected in any of the uninoculated varieties. In inoculated varieties, L. xyli subsp. xyli was not detected by PCR in leaf extracts but was detected by EB-EIA, TB-EIA and PCR in stalk extracts from all the varieties in both plant crop and ratoon crop. Evaporative-blot enzyme immunoassay is recommended for quarantine detection of RSD bacterium in stalk because it is simple, less costly, sap used for detection can be stored for a long time and result read rapidly with a microplate reader compared to counting visually with stereo microscope in TB-EIA. PCR is much more expensive and accurate; it can be used to confirm results that are either questionable or negative from these serological techniques.Keywords: Ratoon stunting disease, Leifsonia xyli subsp. xyli detection, Sugarcane varieties, Nigeri

Evaluation of crude watermelon oil as lubricant in cylindrical turning of AISI 1525 steel employing Taguchi and grey relational analyses techniques

Cutting fluids are used for cooling and lubricating the machining area of components used in manufacturing industries such as aerospace, automotive, petroleum, and heavy machinery. Mineral oils derived from petroleum are commonly utilized as cutting fluids. Mineral oil is hazardous to the health of workers and damaging to the environment. There is a need for a substitute for mineral oil. Vegetable oil is increasingly being used as a cutting fluid. Vegetable oils are easily accessible and have benefits including excellent biodegradability, resistance to fire, low humidity rates, and a low coefficient of expansion under heat. This study adopts watermelon oil as a lubricant in MQL machining of AISI 1525 steel using tungsten tools. In the experiment, the feed rate, depth of cut (DC) and spindle speed were varied using the Taguchi L9 orthogonal array. Grey relational analysis was conducted to obtain optimum cutting parameters for surface roughness, machine vibration, and cutting temperature. Hardness and microstructural analysis of the workpiece were also conducted. Results showed that vegetable oil performed much more effectively than mineral oil in most experiments. The DC was shown to be the most efficient cutting parameter after applying ANOVA analysis based on the parameters that were evaluated. Additionally, models for cutting temperature, machine vibration, and surface roughness values have been developed with accuracy between 69.73 % and 99.05 %. The hardness of the workpiece increases with an increase in diameter, which was attributed to the increase in the steel rod (workpiece) cross-sectional area and the likelihood of a more uniform stress distribution. Moreover, finer grain sizes were observed at 70 mm diameter, with the predominant presence of pearlites. These characteristics were reportedly beneficial to the material's toughness and strength

Surveillance for African Swine fever in Nigeria, 2006-2009

African swine fever (ASF) has had significant economic and social impact in Nigeria since 1997. However, there has been no effective national response to bring it under control. In this report, we confirm that ASF is still prevalent and widespread in Nigeria. Results from both serosurveillance and virological analyses indicated that ASF is present in most of the agro-ecological zones of the country. Nine per cent (9%) of serum samples and 48% of tissue samples were positive for ASF virus antibody and genome, respectively. Areas with high pig-related activities (marketing, consumption and farming) have higher prevalences compared with areas with less pig activities. Farm-gate buyers, marketing systems and transport of untested pigs within the country assist with the circulation of the virus. Only by putting in place a comprehensive routine surveillance and testing system, reorganizing the market and transportation systems for pigs, implementing on-farm bio-security protocols and considering the option of compensation will it be possible to achieve a significant reduction in ASF prevalence in Nigeria