Screening of Fascaplysin for its Efficient Abatement of Hepatocellular Carcinoma and Promiscuous Epitope Mapping of Selected Proteins with Docking Perspectives.

Hepatocellular carcinoma (HCC) is regarded as one among the deadliest cancers in the world. In particular, aflatoxin induced hepatocellular carcinoma is alarmingly on rise due to food contamination. However, several drugs and synthetic compound are reported to inhibit HCC in humans. Fascaplysin is a marine sponge derived compound has been increasingly considered and proved as a potential inhibitor of CDK2/CDK4 dependent kinase. In the present study, five proteins that are involved in activation of aflatoxin B1 induced hepatocellular carcinogenesis were selected and analyzed for its immunological profiling. Crystal structure of Human Glutathione S-Transferase (GST) A1, Prostaglandin H2 Synthase (PHS), Human Cytochrome p450 3A4, Human Microsomal P450 1A2 and p53 were assessed for their immunogenicity patterns. On the other hand, HLA B27 allele which plays a crucial role in cancer was chosen for T cell and B cell epitope mapping. In this analysis, it shows that SYFPEITHI immunogenic peptides were conserved in all the proteins envisaging the need for a common anti-cancer ligand. However, immunogenic assessment of epitopes and interacting residues revealed that fascaplysin interacts at multiple positions in binding amino acid residues of the selected proteins. This study is purely based on the immunoinformatics approach for the screening of specific compound which could suppress the hepatocellular carcinoma. Therefore, based on the results it’s clear that fascaplysin is a potential inhibitor and effectively binds to immunogenic peptides and act as a candidate against aflatoxin induced hepatocellular carcinoma based on the Insilco analysis

Efficacy of partial purified bacteriocin of Pseudomonas aeruginosa on Methicillin-resistant Staphylococcus aureus biofilm

Biofilms are microbial communities that cause serious chronic infections in the environment by enhancing antimicrobial resistance. Bacteria in the biofilm can be up to a thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. The emergence of antibiotic-resistant microorganism has led to the exploration of different therapeutic agents like ribosomally synthesized microorganism peptides referred to as bacteriocins. In this study, bacteriocin producing bacteria Pseudomonas aeruginosa isolated from a soil sample. It was found to be effective against Methicillin-resistant Staphylococcus aureus (MRSA). Furthermore the bacteriocin was partial purified by ammonium sulfate, the precipitate has highly effective against MRSA (400AU/mL). MRSA cells were treated with precipitated culture supernatant of P. aeruginosa TA6 was analyzed by FT-IR. The treated and untreated MRSA showed band variations at 682.59 and 3442.15cm-1 corresponding to the alkyl and amide group respectively. Bacteriocin showed marked inhibition activity against the biofilm of MRSA. About 0.05% and 0.02% attachment of biofilm was observed in the presence of 1X MIC (10 μg/mL) and 2X MIC (20 g/mL) respectively. Our results recommend that bacteriocins that make stable pores on biofilm cells are extremely potent for the treatment of MRSA biofilm infections

Biologically Synthesized Silver Nanoparticles and Their Diverse Applications

Nanotechnology has become the most effective and rapidly developing field in the area of material science, and silver nanoparticles (AgNPs) are of leading interest because of their smaller size, larger surface area, and multiple applications. The use of plant sources as reducing agents in the fabrication of silver nanoparticles is most attractive due to the cheaper and less time-consuming process for synthesis. Furthermore, the tremendous attention of AgNPs in scientific fields is due to their multiple biomedical applications such as antibacterial, anticancer, and anti-inflammatory activities, and they could be used for clean environment applications. In this review, we briefly describe the types of nanoparticle syntheses and various applications of AgNPs, including antibacterial, anticancer, and larvicidal applications and photocatalytic dye degradation. It will be helpful to the extent of a better understanding of the studies of biological synthesis of AgNPs and their multiple uses