178 research outputs found

    Bombyxin (Bombyx Insulin-Like Peptide) Increases the Respiration Rate Through Facilitation of Carbohydrate Catabolism in Bombyx mori

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    Bombyxin-II, an insulin-like peptide of the silkmoth Bombyx mori, has been shown to reduce both the trehalose concentration in the hemolymph and the glycogen content in some tissues of B. mori larvae. However, little is known about how these storage carbohydrates are utilized. To address this question, the effects of bombyxin-II injection into Bombyx larvae on the tissue lipid level, respiration rate, and glycolytic activity of tissues were investigated. Bombyxin-II did not affect lipid accumulation in the hemolymph and fat body, while it increased the rate of oxygen consumption and increased the content of fructose 2, 6-bisphosphate, a potent activator of glycolysis, in the gonads, imaginal discs, and midgut. These results suggest that bombyxin facilitates cellular energy production thereby supporting the tissue growth of insects

    High DNA sequence diversity in pericentromeric genes of the plant Arabidopsis lyrata

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    Differences in neutral diversity at different loci are predicted to arise due to differences in mutation rates and from the “hitchhiking” effects of natural selection. Consistent with hitchhiking models, Drosophila melanogaster chromosome regions with very low recombination have unusually low nucleotide diversity. We compared levels of diversity from five pericentromeric regions with regions of normal recombination in Arabidopsis lyrata, an outcrossing close relative of the highly selfing A. thaliana. In contrast with the accepted theoretical prediction, and the pattern in Drosophila, we found generally high diversity in pericentromeric genes, which is consistent with the observation in A. thaliana. Our data rule out balancing selection in the pericentromeric regions, suggesting that hitchhiking is more strongly reducing diversity in the chromosome arms than the pericentromere regions

    ASTE CO(3-2) Observations of the Barred Spiral Galaxy M 83: I. Correlation between CO(3-2)/CO(1-0) Ratios and Star Formation Efficiencies

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    We present CO(J=3-2) emission observations with the Atacama Submillimeter Telescope Experiment (ASTE) toward the 5' x 5' (or 6.6 x 6.6 kpc at the distance D = 4.5 Mpc) region of the nearby barred spiral galaxy M 83. We successfully resolved the major structures, i.e., the nuclear starburst region, bar, and inner spiral arms in CO(J=3-2) emission at a resolution of 22'' (or 480 pc), showing a good spatial coincidence between CO(J=3-2) and 6 cm continuum emissions. We found a global CO(J=3-2) luminosity L'_CO(3-2) of 5.1 x 10^8 K km s^-1 pc^2 within the observed region. We also found L'_CO(3-2) in the disk region (0.5 < r < 3.5 kpc) of 4.2 x 10^8 K km s^-1 pc^2, indicating that CO(J=3-2) emission in the disk region significantly contributes to the global L'_CO(3-2). From a comparison of a CO(J=3-2) data with CO(J=1-0) intensities measured with Nobeyama 45-m telescope, we found that the radial profile of CO(J=3-2)/CO(J=1-0) integrated intensity ratio R_3-2/1-0 is almost unity in the central region (r < 0.25 kpc), whereas it drops to a constant value, 0.6--0.7, in the disk region. The radial profile of star formation efficiencies (SFEs), determined from 6 cm radio continuum and CO(J=1-0) emission, shows the same trend as that of R_3-2/1-0. At the bar-end (r ~ 2.4 kpc), the amounts of molecular gas and the massive stars are enhanced when compared with other disk regions, whereas there is no excess of R_3-2/1-0 and SFE in that region. This means that a simple summation of the star forming regions at the bar-end and the disk cannot reproduce the nuclear starburst of M 83, implying that the spatial variation of the dense gas fraction traced by R_3-2/1-0 governs the spatial variation of SFE in M 83.Comment: 13 pages, 11 figures, PASJ in press, version with high resolution figures is available via http://www.nro.nao.ac.jp/~z5001km/m83-aste.pd

    Epigenetic regulation of spurious transcription initiation in Arabidopsis

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    In plants, epigenetic regulation is critical for silencing transposons and maintaining proper gene expression. However, its impact on the genome-wide transcription initiation landscape remains elusive. By conducting a genome-wide analysis of transcription start sites (TSSs) using cap analysis of gene expression (CAGE) sequencing, we show that thousands of TSSs are exclusively activated in various epigenetic mutants of Arabidopsis thaliana and referred to as cryptic TSSs. Many have not been identified in previous studies, of which up to 65% are contributed by transposons. They possess similar genetic features to regular TSSs and their activation is strongly associated with the ectopic recruitment of RNAPII machinery. The activation of cryptic TSSs significantly alters transcription of nearby TSSs, including those of genes important for development and stress responses. Our study, therefore, sheds light on the role of epigenetic regulation in maintaining proper gene functions in plants by suppressing transcription from cryptic TSSs

    Direct Observation of Broadband Coating Thermal Noise in a Suspended Interferometer

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    We have directly observed broadband thermal noise in silica/tantala coatings in a high-sensitivity Fabry-Perot interferometer. Our result agrees well with the prediction based on indirect, ring-down measurements of coating mechanical loss, validating that method as a tool for the development of advanced interferometric gravitational-wave detectors.Comment: Final version synchronized with publication in Phys. Lett.

    Construction of Cardiac Tissue Rings Using a Magnetic Tissue Fabrication Technique

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    Here we applied a magnetic force-based tissue engineering technique to cardiac tissue fabrication. A mixture of extracellular matrix precursor and cardiomyocytes labeled with magnetic nanoparticles was added into a well containing a central polycarbonate cylinder. With the use of a magnet, the cells were attracted to the bottom of the well and allowed to form a cell layer. During cultivation, the cell layer shrank towards the cylinder, leading to the formation of a ring-shaped tissue that possessed a multilayered cell structure and contractile properties. These results indicate that magnetic tissue fabrication is a promising approach for cardiac tissue engineering

    A Search for CO(J=3-2) Emission from the Host Galaxy of GRB 980425 with the Atacama Submillimeter Telescope Experiment

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    We report on a deep search for CO(J=3-2) line emission from the host galaxy of GRB 980425 with the Atacama Submillimeter Telescope Experiment (ASTE). We observed five points of the galaxy covering the entire region. After combining all of the spectra, we obtained a global spectrum with the rms noise level of 3.3 mK in T_mb scale at a velocity resolution of 10 km s^-1. No significant emission was detected, though we find a marginal emission feature in the velocity range corresponding to the redshift of the galaxy. We derive 3 sigma upper limits on the global properties: the velocity-integrated CO(3-2) intensity of I_CO(3-2) < 0.26 K km s^-1 by adopting a velocity width of 67 km s^-1; the H_2 column density of N(H_2) < 3 x 10^20 cm^-2; the molecular gas mass of M(H_2) < 3 x 10^8 M_sun, by assuming a CO line luminosity to H_2 molecular gas mass conversion factor of X_CO = 5.0 x 10^20 cm^-2 (K km s^-1)^-1; and the star formation rate of SFR < 0.1 M_sun yr^-1, based on the Schmidt law. The SFR is consistent with the previous results of H_alpha and mid-IR observations, thereby suggesting that there is no significant obscured star formation in the host galaxy of GRB 980425. This result implies that there is a variety of GRB hosts with regard to the presence of obscured star formation.Comment: 7 pages, 4 figure

    Coincidence analysis to search for inspiraling compact binaries using TAMA300 and LISM data

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    Japanese laser interferometric gravitational wave detectors, TAMA300 and LISM, performed a coincident observation during 2001. We perform a coincidence analysis to search for inspiraling compact binaries. The length of data used for the coincidence analysis is 275 hours when both TAMA300 and LISM detectors are operated simultaneously. TAMA300 and LISM data are analyzed by matched filtering, and candidates for gravitational wave events are obtained. If there is a true gravitational wave signal, it should appear in both data of detectors with consistent waveforms characterized by masses of stars, amplitude of the signal, the coalescence time and so on. We introduce a set of coincidence conditions of the parameters, and search for coincident events. This procedure reduces the number of fake events considerably, by a factor 104\sim 10^{-4} compared with the number of fake events in single detector analysis. We find that the number of events after imposing the coincidence conditions is consistent with the number of accidental coincidences produced purely by noise. We thus find no evidence of gravitational wave signals. We obtain an upper limit of 0.046 /hours (CL =90= 90 %) to the Galactic event rate within 1kpc from the Earth. The method used in this paper can be applied straightforwardly to the case of coincidence observations with more than two detectors with arbitrary arm directions.Comment: 28 pages, 17 figures, Replaced with the version to be published in Physical Review

    Results of the search for inspiraling compact star binaries from TAMA300's observation in 2000-2004

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    We analyze the data of TAMA300 detector to search for gravitational waves from inspiraling compact star binaries with masses of the component stars in the range 1-3Msolar. In this analysis, 2705 hours of data, taken during the years 2000-2004, are used for the event search. We combine the results of different observation runs, and obtained a single upper limit on the rate of the coalescence of compact binaries in our Galaxy of 20 per year at a 90% confidence level. In this upper limit, the effect of various systematic errors such like the uncertainty of the background estimation and the calibration of the detector's sensitivity are included.Comment: 8 pages, 4 Postscript figures, uses revtex4.sty The author list was correcte
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