Reliability analysis of tunnel final lining

Risk and Reliability in Geotechnical Engineerin

Radar interferometry based settlement monitoring in tunnelling: visualisation and accuracy analyses

Background The accurate, efficient and economical monitoring of settlements caused by tunnel boring machines, especially in regions of particular interest such as critical inner city areas, has become an important aspect of the tunnelling operation. Besides conventional terrestrial based methods to capture settlements, satellite based techniques that can accurately determine displacements remotely, are increasingly being used to augment standard terrestrial measurements. However, not much attention has been paid to analyse the accuracy of satellite based measurement data. In addition, there is also a lack of studies on how to visualise the resulting huge amount of data in the context of both the tunnel advancement and the existing building infrastructure. Methods This paper introduces the basics of settlement monitoring using radar interferometry methods, in particular showing the results obtained by processing radar images from the TerraSAR-X satellite to monitor a downtown construction site in Düsseldorf, Germany, where a new underground line (“Wehrhahn-Linie”) is being built. By comparing terrestrial measurements with remote satellite based settlement data in temporal and spatial corridors, the accuracy of the radar interferometry method is shown. Moreover, a 4D visualisation concept is presented that correlates satellite and terrestrial based settlement data correlated with above-ground buildings and boring machine performance parameters within a Virtual Reality (VR) environment. Results By comparing up to 23,000 pairs of satellite and terrestrial based settlement data points of a real tunnelling project an accuracy of about ±1.5 mm in the measurement of deformation using the method of radar interferometry in urban areas can be stated. In addition, providing a visual analysis of data sources within a VR environment, the accuracy of terrestrial and satellite-based measurements can be visualised in different time steps. Sources of error that affect the degree of accuracy, such as atmospheric conditions, systematic errors in the evaluation of radar images and local events in the spatial corridor, can be quantified. In addition, the 4D visualisation can help reveal direct interdependencies between settlement data and boring machine performance data. Conclusions The Persistent Scatterer Interferometry (PSI) based on high resolution radar images of the TerraSAR-X satellite, in combination with conventional ground-based terrestrial measurements, provides a new settlement monitoring approach in tunnelling. For example, due to minimized surveying works and disruptions of construction activities on site and due to the large settlement area coming with a high magnitude of settlement data points, this combined monitoring approach is very practical and economical. Moreover, by visualizing the settlement data properly, the risk of damage of surface structures can be analysed and understood more precisely, which increases the safety of underground works

Discoidin domain receptor-1 and periostin: New players in chronic kidney disease

The incidence and prevalence of chronic kidney disease represents an important problem for public health. In renal diseases, the main histologic alterations derive from the development of renal fibrosis which results from the loss of the balance between proand anti-fibrotic factors. Tyrosine kinase receptors (RTKs) and matricellular proteins (MPs) are nowadays studied as potential modulators of renal injury. RTKs regulate cell cycle, migration, metabolism and cellular differentiation. Discoidin domain receptor-1 (DDR-1) is an RTK that has been extensively studied in cancer, and lung and renal diseases. It modulates inflammatory recruitment, extracellular matrix deposition and fibrosis; in renal diseases, it appears to act independently of the underlying disease. MPs regulate cell-matrix interactions and matrix accumulation, cellular adhesion and migration, and expression of inflammatory cells. Periostin is an MP, mainly studied in bone, heart, lung and cancer. Several studies demonstrated that it mediates cellmatrix interactions, migration of inflammatory cells and development of fibrosis. Recently, it has been reported in several nephropathies. In this review, we discuss the potential pathological roles of DDR-1 and periostin focussing on the kidney in both experimental models and human diseases

Blocking Connexin-43 mediated hemichannel activity protects against early tubular injury in experimental chronic kidney disease

Background: Tubulointerstitial fibrosis represents the key underlying pathology of Chronic Kidney Disease (CKD), yet treatment options remain limited. In this study, we investigated the role of connexin43 (Cx43) hemichannel-mediated adenosine triphosphate (ATP) release in purinergic-mediated disassembly of adherens and tight junction complexes in early tubular injury. Methods: Human primary proximal tubule epithelial cells (hPTECs) and clonal tubular epithelial cells (HK2) were treated with Transforming Growth Factor Beta1 (TGFβ1) ± apyrase, or ATPγS for 48h. For inhibitor studies, cells were co-incubated with Cx43 mimetic Peptide 5, or purinergic receptor antagonists Suramin, A438079 or A804598. Immunoblotting, single-cell force spectroscopy and trans-epithelial electrical resistance assessed protein expression, cell-cell adhesion and paracellular permeability. Carboxyfluorescein uptake and biosensing measured hemichannel activity and real-time ATP release, whilst a heterozygous Cx43+/- mouse model with unilateral ureteral obstruction (UUO) assessed the role of Cx43 in vivo. Results: Immunohistochemistry of biopsy material from patients with diabetic nephropathy confirmed increased expression of purinergic receptor P2X7. TGFβ1 increased Cx43 mediated hemichannel activity and ATP release in hPTECs and HK2 cells. The cytokine reduced maximum unbinding forces and reduced cell-cell adhesion, which translated to increased paracellular permeability. Changes were reversed when cells were co-incubated with either Peptide 5 or P2-purinoceptor inhibitors. Cx43+/- mice did not exhibit protein changes associated with early tubular injury in a UUO model of fibrosis. Conclusion: Data suggest that Cx43 mediated ATP release represents an initial trigger in early tubular injury via its actions on the adherens and tight junction complex. Since Cx43 is highly expressed in nephropathy, it represents a novel target for intervention of tubulointerstitial fibrosis in CKD