Human Ghrelin Ameliorates Organ Injury and Improves Survival after Radiation Injury Combined with Severe Sepsis

In the terrorist radiation exposure scenario, radiation victims are likely to suffer from additional injuries such as sepsis. Our previous studies have shown that ghrelin is protective in sepsis. However, it remains unknown whether ghrelin ameliorates sepsis-induced organ injury and mortality after radiation exposure. The purpose of this study is to determine whether human ghrelin attenuates organ injury and improves survival in a rat model of radiation combined injury (RCI) and, if so, the potential mechanism responsible for the benefit. To study this, adult male rats were exposed to 5-Gy whole body irradiation followed by cecal ligation and puncture (CLP, a model of sepsis) 48 h thereafter. Human ghrelin (30 nmol/rat) or vehicle (saline) was infused intravenously via an osmotic minipump immediately after radiation exposure. Blood and tissue samples were collected at 20 h after RCI (68 h after irradiation or 20 h after CLP) for various measurements. To determine the longterm effect of human ghrelin after RCI, the gangrenous cecum was removed at 5 h after CLP and 10-d survival was recorded. In addition, vagotomy or sham vagotomy was performed in sham and RCI animals immediately prior to ghrelin administration, and various measurements were performed at 20 h after RCI. Our results showed that serum levels of ghrelin and its gene expression in the stomach were decreased markedly at 20 h after RCI. Administration of human ghrelin attenuated tissue injury markedly, reduced proinflammatory cytokine levels, decreased tissue myeloperoxidase activity, and improved survival after RCI. Furthermore, elevated plasma levels of norepinephrine (NE) after RCI were reduced significantly by ghrelin. However, vagotomy prevented ghrelin’s beneficial effects after RCI. In conclusion, human ghrelin is beneficial in a rat model of RCI. The protective effect of human ghrelin appears to be attributed to re-balancing the dysregulated sympathetic/parasympathetic nervous systems

Abstracts of the International Conference on Recent Trends in Mathematics and Computer Science 2023

This book presents the abstracts of the selected contributions to the International Conference on Recent Trends in Mathematics and Computer Science 2023 (ICRTMCS-2023), held on 19-21 October 2023 by the Auxilium College of Arts and Science for Women, Regunathapuram, Tamil Nadu, India. ICRTMCS-2023 was a multidisciplinary conference organized with the objective of bringing together eminent academicians, research scholars, and students to exchange ideas, communicate, to discuss research findings and new advances on recent and emerging trends in the field of Mathematics and Computer Science. Moreover, the conference would also enable the participants to explore new fields and gain immense knowledge. Conference Title: International Conference on Recent Trends in Mathematics and Computer Science 2023Conference Acronym: ICRTMCS-2023Conference Date: 19-21 October 2023Conference Venue: Hybrid (Online and Auxilium College of Arts and Science for Women, Regunathapuram, India)Conference Organizer: Departments of Mathematics and Computer Science, Auxilium College of Arts and Science for Women, Regunathapuram, Tamil Nadu, Indi