16 research outputs found
Decalcification: An Amenable Process in Oral Histology
In histopathology, decalcification of calcified tissues plays a highly essential role in determining any hard tissue pathology by means of adequate tissue processing protocols. Before the examination of the tissue thoroughly to thrive for a final diagnosis, tissues go through a sequential curriculum to be differentiated clearly. To prepare tissues for microscopic examination, the elimination of calcium from hard tissues is an essential step so that acceptable sections can be obtained and used further. Decalcification is carried out by various decalcifying methods incorporating a variety of decalcifying agents, including acids and chelating agents. However, as it sometimes takes a long time lag and decreases the staining qualities of the specimen, many ventures have been made to find methods for accelerating this procedure
AIE+ESIPT Active Hydroxybenzothiazole for Intracellular Detection of Cu2+: Anticancer and Anticounterfeiting Applications
Here, in the present work, a new hydroxybenzothiazole derivative (HBT 2) with AIE+ESIPT features was synthesized by Suzuki–Miyora coupling of HBT 1 with 4-formylphenylboronic acid. The AIE and ESIPT features were confirmed by optical, microscopic (AFM) and dynamic light scattering (DLS) techniques. The yellow fluorescent aggregates of HBT 2 can specifically detect Cu2+/Cu+ ions with limits of detection as low as 250 nM and 69 nM. The Job’s plot revealed the formation of a 1:1 complex. The Cu2+ complexation was further confirmed by optical, NMR, AFM and DLS techniques. HBT 2 was also used for the detection of Cu2+ ions in real water samples collected from different regions of Punjab. HBT 2 was successfully used for the bio-imaging of Cu2+ ions in live A549 and its anticancer activity was checked on different cancer cell lines, such as MG63, and HeLa, and normal cell lines such as L929. We successfully utilized HBT 2 to develop security labels for anticounterfeiting applications
Atypical CT Findings in Plexiform Ameloblastoma
Ameloblastoma is an uncommon epithelial odontogenic neoplasm that is nonmineralized, locally aggressive, and, in most cases, benign. Most ameloblastomas develop in the molar-ramus region of the mandible with 70% of them arising in the molar-ramus area. Radiologically they are unilocular or multilocular radiolucency with a honeycomb or soap bubble appearance. The radiographic appearance of ameloblastoma can vary according to the type of tumor. CT is usually helpful in determining the contours of the lesion, its contents, and its extension into soft and hard tissues. Through this case we would bring to light some of the unusual CT findings which include the destruction of the surrounding structures by the lesion which appeared to be normal routine lesion when viewed clinically
Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from Cassia fistula L. Leaves
Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from C. fistula leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), n-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of C. fistula leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on 1H, 13C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI50 value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G0/G1 phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells
Ultratrace Detection of Nitroaromatics: Picric Acid Responsive Aggregation/Disaggregation of Self-Assembled <i>p</i>‑Terphenylbenzimidazolium-Based Molecular Baskets
1-(<i>p</i>-Terphenyl)-benzimidazolium
(TRIPOD-TP) molecules undergo self-assembly to form rodlike structures
in aqueous medium, as shown by field-emission scanning electron microscopy,
transmission electron microscopy, and dynamic light scattering studies.
Upon gradual addition of picric acid (PA), these aggregates undergo
an aggregation/disaggregation process to complex morphological structures
(10<sup>–12</sup>–10<sup>–10</sup> M PA) and
spherical aggregates (10<sup>–9</sup>–10<sup>–8</sup> M PA). These spherical aggregates undergo further dissolution to
well-dispersed spheres between 10<sup>–7</sup>–10<sup>–6</sup> M PA. During fluorescence studies, these aggregates
demonstrate superamplified fluorescence quenching (>97%) in the
presence of 10<sup>–5</sup> to 0.2 equiv of the probe concentration,
an unprecedented process with PA. The lowest detection limits by solution
of TRIPOD-TP are 5 × 10<sup>–13</sup> PA, 50 × 10<sup>–12</sup> M 2,4-dinitrophenol, 200 × 10<sup>–12</sup> M 2,4,6-trinitrotoluene, and 1 nM 1-chloro-2,4-dinitrobenzene. Paper
strips dipped in the solution of TRIPOD-TP demonstrate quantitative
fluorescence quenching between 10<sup>–17</sup> and 10<sup>–6</sup> M PA using front-surface steady state studies and
can measure as low as 2.29 × 10<sup>–20</sup> g/cm<sup>2</sup> PA