427 research outputs found
Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation
We thank members of the Donaldson, Kubota, and Lorenz labs for helpful discussion, Sophie Shaw at the University of Aberdeen for data upload to Array Express and Shin-ichiro Hiraga for help with Bioinformatic analysis. This work was supported by BBSRC Grant BB/K006304/1 and Cancer Research UK Programme Award A19059 to ADD, and Wellcome Trust Grant 095062 to TOH. KS was supported by Grant-in-Aid for Scientific Research on Priority Areas (15H05970 and 15K21761) from Ministry of Education, Culture, Sports, Science and Technology, Japan All raw-data files for MNase-Seq and ChIP-Seq data are uploaded to Array Express under accession number: E-MTAB-6985.Peer reviewedPublisher PD
Abstracts from the 50th European Society of Human Genetics Conference: Posters
International audienc
Genome-organizing factors Top2 and Hmo1 prevent chromosome fragility at sites of S phase transcription
Specialized topoisomerases solve the topological constraints arising when replication forks encounter transcription. We have investigated the contribution of Top2 in S phase transcription. Specifically in S phase, Top2 binds intergenic regions close to transcribed genes. The Top2-bound loci exhibit low nucleosome density and accumulate gammaH2A when Top2 is defective. These intergenic loci associate with the HMG protein Hmo1 throughout the cell cycle and are refractory to the histone variant Htz1. In top2 mutants, Hmo1 is deleterious and accumulates at pericentromeric regions in G2/M. Our data indicate that Top2 is dispensable for transcription and that Hmo1 and Top2 bind in the proximity of genes transcribed in S phase suppressing chromosome fragility at the M-G1 transition. We propose that an Hmo1-dependent epigenetic signature together with Top2 mediate an S phase architectural pathway to preserve genome integrity
Mid-Rapidity Direct-Photon Production in p+p Collisions at sqrt(s) = 200 GeV
A measurement of direct photons in p+p collisions at sqrt(s)=200 GeV is
presented. A photon excess above background from pi^0 --> gamma+gamma, eta -->
gamma+gamma, and other decays is observed in the transverse momentum range 5.5
< p_T < 7 GeV/c. The result is compared to a next-to-leading-order perturbative
QCD calculation. Within errors, good agreement is found between the QCD
calculation and the measured result.Comment: 330 authors, 7 pages text, RevTeX, 2 figures, 2 tables. Submitted to
Physical Review D. Plain text data tables for the points plotted in figures
for this and previous PHENIX publications are (or will be) publicly available
at http://www.phenix.bnl.gov/papers.htm
Suppressed pi^0 Production at Large Transverse Momentum in Central Au+Au Collisions at sqrt(s_NN) = 200 GeV
Transverse momentum spectra of neutral pions in the range 1 < p_T < 10 GeV/c
have been measured at mid-rapidity by the PHENIX experiment at RHIC in Au+Au
collisions at sqrt(s_NN) = 200 GeV. The pi^0 multiplicity in central reactions
is significantly below the yields measured at the same sqrt(s_NN) in peripheral
Au+Au and p+p reactions scaled by the number of nucleon-nucleon collisions. For
the most central bin, the suppression factor is ~2.5 at p_T = 2 GeV/c and
increases to ~4-5 at p_T ~= 4 GeV/c. At larger p_T, the suppression remains
constant within errors. The deficit is already apparent in semi-peripheral
reactions and increases smoothly with centrality.Comment: 326 authors, 6 pages text, RevTeX, 3 figures, 2 tables. Submitted to
PRL. Plain text data tables for the points plotted in figures for this and
previous PHENIX publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Deuteron and antideuteron production in Au+Au collisions at sqrt(s_NN)=200 GeV
The production of deuterons and antideuterons in the transverse momentum
range 1.1 < p_T < 4.3 GeV/c at mid-rapidity in Au + Au collisions at
sqrt(s_NN)=200 GeV has been studied by the PHENIX experiment at RHIC. A
coalescence analysis comparing the deuteron and antideuteron spectra with those
of protons and antiprotons, has been performed. The coalescence probability is
equal for both deuterons and antideuterons and increases as a function of p_T,
which is consistent with an expanding collision zone. Comparing (anti)proton
yields p_bar/p = 0.73 +/- 0.01, with (anti)deuteron yields: d_bar/d = 0.47 +/-
0.03, we estimate that n_bar/n = 0.64 +/- 0.04.Comment: 326 authors, 6 pages text, 5 figures, 1 Table. Submitted to PRL.
Plain text data tables for the points plotted in figures for this and
previous PHENIX publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres where it provides the cohesive counterforce to bipolar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences transiently split in pre-anaphase cells, in some organisms up to several micrometers. This ‘centromere breathing’ presents a paradox, how sister sequences separate where cohesin is most enriched. We now show that in the budding yeast Saccharomyces cerevisiae, cohesin binding diminishes over centromeric sequences that split during breathing. We see no evidence for cohesin translocation to surrounding sequences, suggesting that cohesin is removed from centromeres during breathing. Two pools of cohesin can be distinguished. Cohesin loaded before DNA replication, which has established sister chromatid cohesion, disappears during breathing. In contrast, cohesin loaded after DNA replication is partly retained. As sister centromeres re-associate after transient separation, cohesin is reloaded in a manner independent of the canonical cohesin loader Scc2/Scc4. Efficient centromere re-association requires the cohesion establishment factor Eco1, suggesting that re-establishment of sister chromatid cohesion contributes to the dynamic behaviour of centromeres in mitosis. These findings provide new insights into cohesin behaviour at centromeres
Scaling properties of proton and anti-proton production in sqrt(s_NN) = 200 GeV Au + Au collisions
We report on the yield of protons and anti-protons, as a function of
centrality and transverse momentum, in Au+Au collisions at sqrt(s_NN) = 200 GeV
measured at mid-rapidity by the PHENIX experiment at RHIC. In central
collisions at intermediate transverse momenta (1.5 < p_T < 4.5 GeV/c) a
significant fraction of all produced particles are protons and anti-protons.
They show a centrality-scaling behavior different from that of pions. The
p-bar/pion and p/pion ratios are enhanced compared to peripheral Au+Au, p+p,
and electron+positron collisions. This enhancement is limited to p_T < 5 GeV/c
as deduced from the ratio of charged hadrons to pi^0 measured in the range 1.5
< p_T < 9 GeV/c.Comment: 325 authors, 6 pages text, 4 figures, RevTeX 4. Minor changes to text
and figures to meet PRL length restrictions; no changes to figures;
resubmitted to PRL. Plain text data tables for the points plotted in figures
for this and previous PHENIX publications are (or will be) publicly available
at http://www.phenix.bnl.gov/papers.htm
A Detailed Study of High-pT Neutral Pion Suppression and Azimuthal Anisotropy in Au+Au Collisions at \sqrt{s_{NN}} = 200 GeV
Measurements of neutral pion production at midrapidity in sqrt(s_NN) = 200
GeV Au+Au collisions as a function of transverse momentum, p_T, collision
centrality, and angle with respect to reaction plane are presented. The data
represent the final pi^0 results from the PHENIX experiment for the first RHIC
Au+Au run at design center-of-mass-energy. They include additional data
obtained using the PHENIX Level-2 trigger with more than a factor of three
increase in statistics over previously published results for p_T > 6 GeV/c. We
evaluate the suppression in the yield of high-p_T pi^0's relative to point-like
scaling expectations using the nuclear modification factor R_AA. We present the
p_T dependence of R_AA for nine bins in collision centrality. We separately
integrate R_AA over larger p_T bins to show more precisely the centrality
dependence of the high-p_T suppression. We then evaluate the dependence of the
high-p_T suppression on the emission angle \Delta\phi of the pions with respect
to event reaction plane for 7 bins in collision centrality. We show that the
yields of high-p_T pi^0's vary strongly with \Delta\phi, consistent with prior
measurements. We show that this variation persists in the most peripheral bin
accessible in this analysis. For the peripheral bins we observe no suppression
for neutral pions produced aligned with the reaction plane while the yield of
pi^0's produced perpendicular to the reaction plane is suppressed by more than
a factor of 2. We analyze the combined centrality and \Delta\phi dependence of
the pi^0 suppression in different p_T bins using different possible
descriptions of parton energy loss dependence on jet path-length averages to
determine whether a single geometric picture can explain the observed
suppression pattern.Comment: 330 authors, pages text, RevTeX4, figures, tables. Submitted to
Physical Review C. Plain text data tables for the points plotted in figures
for this and previous PHENIX publications are (or will be) publicly available
at http://www.phenix.bnl.gov/papers.htm
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