The applications of infrared thermography in surgical removal of retained teeth effects assessment

during filling cavities, laser treatment and inflammation of periodontal tissues. The biggest problem in thermal imaging was limiting access and rapid change of humidity when the mouth opens during measurements. However, nowadays thermal map of body surface is correlated with inflammatory state changing inner mouth. The aim of this study was to assess the suitability of thermal imaging to localize the inflammation and monitor treatment effects after surgical removal of third retained molar teeth. The cohort consisted of 27 patients with referral for the surgical extraction of the third retained molar. Thermal imaging of the sagittal face was performed before and after the procedure and on the first, fourth and seventh day after the extraction. Obtained thermal maps are correlated with the third molar teeth inflammation location. Moreover, the changes of temperature in the area of both the tooth and the cheek correspond to the mechanisms of wound healing described in the literature. Obtained results were not only qualitative but also quantitative what was confirmed by statistically significant difference. It seems that thermal imaging, which is a noninvasive method, can be used to monitor treatment processes after surgical procedures, as well as on the location of inflammation

May thermal imaging be useful in the assessment of dentalanaesthesia? : preliminary study

The main goal of this study was to find out if thermal imaging may be useful in the evaluation of two types of anaesthetic injections-with and without a vasoconstrictor. There were 20 patients (13 women and 7 men) involved in the study. The group was divided into two subgroups. Patients from the first subgroup received infiltration anaesthesia (so-called: IA) of lidocaine (2 mL) and the second group included patients, who received infiltration anaesthesia (so-called: IAN) of lidocaine with 2% noradrenaline (2 mL). The obtained results showed a significant increase in the average temperature 10 min after the injection. In the IA group, the temperature increase was nearly 1.0 °C what was 0.3 °C higher than in the IAN group. Moreover, temperature changes showed a wide plateau between 10 and 25 min after anesthesia administration. The effect of temperature rise was also observed on the contralateral cheek where there was no intervention renders by a contralateral reflex

Motive activity as the criterion of efficiency of introduction of the technology which is aimed at the development of professionally significant physical qualities of pupils of clothing manufacture in vocational-technical schools

Purpose: to carry out the analysis of results of researches of the forming experiment, and in particular, indicators of motive activity of respondents. Material & Methods: contingent: pupils of "Balty vocational-technical agrarian school" of Balty of the Odessa Region – 40 girls of the I course who do not go in for sports; methods: analysis of literature, pedagogical methods of the research, questioning, methods of mathematical statistics. Results: the data are analyzed, which are obtained in the forming experiment on the determination of level of motive activity by means of Framingham technique as one of the criteria of efficiency of introduction of the technology, which is directed to the improvement of professionally significant physical qualities of pupils of vocational-technical schools of clothing manufacture. Results of the questioning, which is directed to the identification subjective opinions of respondents concerning their motive activity, are analyzed. The results of questioning of pupils about the main conditions are presented, which are necessary for the involvement of students to the active physical improvement. Conclusions: the received results confirm the efficiency of the developed and introduced technology

The applications of thermal imaging in dental implant – pilot study

Termografia z roku na rok staje się coraz bardziej popularnym narzędziem diagnostycznym w medycynie i biologii. Poprzez analizę obrazów termicznych ciała możliwa jest ocena metabolizmu tkanek podpowierzchniowych. Pozwala to na lokalizację występowania stanów zapalnych oraz weryfikację procesów gojenia się tkanek poddanych zabiegom na podstawie zmian temperatury. Celem niniejszej pracy była próba zastosowania obrazowania termicznego w ocenie stanu zapalnego wywołanego założeniem śruby gojącej w trakcie zabiegu implantacji zęba. Obrazowanie termiczne wykonano przed i po zabiegu oraz w 2 i 12 dobie po zabiegu, oceniając zmiany temperatury w obszarze zabiegu (tzw. obszar zęba), w obszarze policzka po stronie zabiegu (tzw. obszar policzka) oraz w obszarze policzka zdrowego (tj. obszar kontrolny). Badania przeprowadzono na grupie 6 pacjentów, a otrzymane wyniki wskazują na wystąpienie stanu zapalnego po zabiegu, co wydaje się być efektem spodziewanym ze względu na procedury wiercenia i wkręcania śruby. Podwyższona temperatura w 2 dobie po zabiegu wskazuje na utrzymywanie się stanu zapalnego, a jej wyraźny spadek obserwowany jest w 12 dobie, co wskazuje na zakończenie fazy regeneracji i gojenia się tkanek. Wstępne pomiary mogą wskazywać na przydatność metody obrazowania termicznego w implantacji zębów.From year to year thermal imaging is becoming more and more popular diagnostic tool in medicine and biology. The metabolism of subsurface tissues is possible due to the thermal images analysis. Moreover, the inflammation location and tissue healing processes may be verified based on the temperature changes. The aim of study was to try to use thermal imaging in the inflammation assessment, which was caused by healing abutment of a dental implant location. Thermal imaging was done before and after treatment and in 2nd and 12th day after procedure. The assessment of temperature changes was done in procedure location ROI (so-called the tooth area), the cheek on the side of procedure ROI (so-called the cheek area) and the health cheek ROI (so-called the control side). The study included six patients, and the results indicate that inflammation appeared directly after the procedure. In fact, this was expected due to drilling and placing the healing abutment of a dental implant. The increased temperature in 2nd day after procedure may suggest that inflammation is maintained till 12th day, when the healing processes are ending. The preliminary results may indicate the usefulness of thermal imaging in dental implants monitoring

Walnut Male Flowers (Juglans regia L.) as a Functional Addition to Wheat Bread

The aim of this study is to assess the effect of adding dried and ground walnut male flowers to wheat flour on selected physicochemical and antioxidant properties of the obtained wheat bread. The control sample was bread without the addition of walnut male flowers. In the tests, the addition of flowers was used in the amounts of 0.5%, 1.0%, 1.5%, 2.0% and 2.5% of the weight of the flour used in the control sample. Bread dough was prepared according to the direct method with the use of yeast. The parameters of the baking process of the control bread and of the test bread were calculated. The selected parameters determining the quality of the obtained bread, including the loaf volume, the specific volume, the crumb porosity, the crumb texture and the color were measured. The antioxidant potential and total polyphenol content were tested both in the dough and in the bread. The test results were analyzed statistically. It was found that the partial replacement of wheat flour with walnut male flowers resulted in a significant reduction in the loaves’ volume and in the darkening of both the color of the dough and the crumb. The value of the L* parameter decreased with the increasing addition of walnut flowers. The lowest values of this parameter were found to be 53.87 (crust) and 39.94 (crumb) in the sample with 2.5% addition. The average volume of the loaves ranged from 565 cm3 (0.5%) to 675 cm3 (control). The use of the additive in the amount of 2.5% caused significant changes in the values of most of the examined parameters of the bread crumb texture. The addition of walnut male flowers to the flour had a significant effect on the antioxidant potential and the total polyphenol content of the tested doughs and breads. In the case of bread, the total polyphenol content value ranged from 96.90 mg GAE/g dw to 208.08 mg GAE/g dw. Similarly, the antioxidant potential increased with increasing walnut male flower supplementation. The antioxidant potential determined by the ABTS and FRAP methods ranged from 1.42 mmol TE/g dw to 2.02 mmol TE/g dw and from 0.08 mmol TE/g dw to 0.33 mmol TE/g dw, respectively. The obtained research results prove the application potential of walnut flowers in the design of food, with beneficial pro-health properties, and are an example of the use of plant by-products in the process of food enrichment

Biophysical Characterization of Viral and Lipid-Based Vectors for Vaccines and Therapeutics with Light Scattering and Calorimetric Techniques

Novel vaccine platforms for delivery of nucleic acids based on viral and non-viral vectors, such as recombinant adeno associated viruses (rAAV) and lipid-based nanoparticles (LNPs), hold great promise. However, they pose significant manufacturing and analytical challenges due to their intrinsic structural complexity. During product development and process control, their design, characterization, and quality control require the combination of fit-for-purpose complementary analytical tools. Moreover, an in-depth methodological expertise and holistic approach to data analysis are required for robust measurements and to enable an adequate interpretation of experimental findings. Here the combination of complementary label-free biophysical techniques, including dynamic light scattering (DLS), multiangle-DLS (MADLS), Electrophoretic Light Scattering (ELS), nanoparticle tracking analysis (NTA), multiple detection SEC and differential scanning calorimetry (DSC), have been successfully used for the characterization of physical and chemical attributes of rAAV and LNPs encapsulating mRNA. Methods’ performance, applicability, dynamic range of detection and method optimization are discussed for the measurements of multiple critical physical−chemical quality attributes, including particle size distribution, aggregation propensity, polydispersity, particle concentration, particle structural properties and nucleic acid payload.publishedVersio

Biophysical Characterization of Viral and Lipid-Based Vectors for Vaccines and Therapeutics with Light Scattering and Calorimetric Techniques

Novel vaccine platforms for delivery of nucleic acids based on viral and non-viral vectors, such as recombinant adeno associated viruses (rAAV) and lipid-based nanoparticles (LNPs), hold great promise. However, they pose significant manufacturing and analytical challenges due to their intrinsic structural complexity. During product development and process control, their design, characterization, and quality control require the combination of fit-for-purpose complementary analytical tools. Moreover, an in-depth methodological expertise and holistic approach to data analysis are required for robust measurements and to enable an adequate interpretation of experimental findings. Here the combination of complementary label-free biophysical techniques, including dynamic light scattering (DLS), multiangle-DLS (MADLS), Electrophoretic Light Scattering (ELS), nanoparticle tracking analysis (NTA), multiple detection SEC and differential scanning calorimetry (DSC), have been successfully used for the characterization of physical and chemical attributes of rAAV and LNPs encapsulating mRNA. Methods’ performance, applicability, dynamic range of detection and method optimization are discussed for the measurements of multiple critical physical−chemical quality attributes, including particle size distribution, aggregation propensity, polydispersity, particle concentration, particle structural properties and nucleic acid payload

The Content of Phenolic Acids and Flavonols in the Leaves of Nine Varieties of Sweet Potatoes (Ipomoea batatas L.) Depending on Their Development, Grown in Central Europe

The aim of the study was the qualitative and quantitative analysis of the bioactive components present in the leaves of 9 sweet potato cultivars grown in the moderate climate in Poland, which were harvested at different growth stages according to the BBCH (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) scale (14, 51, 89). It was found that sweet potato leaves contained 7 polyphenolic compounds, including 5 chlorogenic acids—neochlorogenic acid (5-CQA), chlorogenic acid (3-CQA), 4-cryptochlorogenic acid (4-CQA), 34-di-O-caffeoylqunic acid (3,4-CQA), 3,5-di-O-caffeoylqunic acid (3,5-CQA)—and 2 flavonoids, quercetin-3-O-galactoside (Q-3-GA) and quercetin-3-O-glucoside (Q-3-GL). Their content depended on the genotype of the examined cultivars and on the stage of leaf development. The mean content of the identified polyphenolic compounds in the examined cultivars ranged from 148.2 to 14.038.6 mg/100 g−1 DM for the leaves harvested at growth stage 14 according to the BBCH scale. In the case of leaves harvested at BBCH stage 51, the concentration of polyphenolic compounds ranged from 144.76 to 5026.8 mg/100 g−1 DM and at BBCH stage 89 from 4078.1 to 11.183.5 mg/100 g−1 DM. The leaves of the Carmen Rubin cultivar collected at stage 14 contained the highest amount of polyphenolic compounds, while Okinava leaves had the highest amount of these compounds at stage 51. The highest content of polyphenolic compounds in leaves at BBCH growth stage 89 was found in the Radiosa variety. The highest concentration levels were found for 3-CQA at all stages of leaf development. Significant correlations between polyphenol content and antioxidant activity measured by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing/antioxidant power (FRAP) were found. The results of this experiment revealed that the growth stages and genetic properties of cultivars have a very significant influence on the content of phenolic acids and flavonols in sweet potato leaves. The results are innovative and can have a practical application, as the knowledge of the content of the substances under study makes it possible to determine the optimal management practice of sweet potato leaf harvest in order to obtain more top-quality raw material