The Impact of Environmental Liabilities on Real Estate Contract Negotiations

The author examines environmental issues facing a prospective seller of industrial real property. Because a seller generally will not be able to transfer statutory responsibility for environmental problems, it is advisable for the seller to investigate and remedy environmental problems prior to transferring the property. An environmental investigation may be required for the seller to make representations and warranties to buyers and lenders. Both lenders and buyers will want to conduct some degree of investigation to protect themselves from environmental liability, particularly liability arising from Superfund and similar state statutes. Information gained from environmental investigations can be useful during negotiations for sale and for contractual allocation of environmental responsibility. Where a site cleanup is required, information gained from an environmental investigation can serve as the basis for a remediation plan. As a result of an environmental investigation and site remediation, the seller will be in a stronger position to negotiate limitations on indemnity provisions requested by the buyer. The author concludes that given the current level of environmental awareness and regulation, cleaning up contaminated industrial property is not only good policy, it is good business

Counting absolute number of molecules using unique molecular identifiers

Advances in molecular biology have made it easy to identify different DNA or RNA species and to copy them. Identification of nucleic acid species can be accomplished by reading the DNA sequence; currently millions of molecules can be sequenced in a single day using massively parallel sequencing. Efficient copying of DNA-molecules of arbitrary sequence was made possible by molecular cloning, and the polymerase chain reaction. Differences in the relative abundance of a large number of different sequences between two or more samples can in turn be measured using microarray hybridization and/or tag sequencing. However, determining the relative abundance of two different species and/or the absolute number of molecules present in a single sample has proven much more challenging. This is because it is hard to detect individual molecules without copying them, and even harder to make defined number of copies of molecules. We show here that this limitation can be overcome by using unique molecular identifiers (umis), which make each molecule in the sample distinct

Stabilization of uranium(VI) at low pH by fungal metabolites : applications in environmental biotechnology

Uranium contamination of soils and water is a worldwide problem due to geology or anthropogenic release such as mining, or use of inorganic fertilizers. In situ remediation of low and moderately contaminated sites is a complicated procedure due to the complex chemistry of uranium. This study demonstrates that at pH 3.5, a fungal strain isolated from unprocessed uranium bearing shale creates hydrochemical conditions that immobilize 97% of a total of 10 mg L-1 dissolved uranium in a 0.20 μm pore system. The redistribution occurred within 10 minutes and remained for five weeks and just 12% of the inventory was retrieved in the biomass. Size exclusion chromatography of the dissolved phase identified organic substances in the range of more than 60 kD down to 100 D as a response to time of incubation. Geochemical modeling indicates formation of uranium-organic complexes where ligand size, coordination chemistry and their tendency to agglomerate determine the redistribution

An updated investigation of the multidimensional structure of language lateralization in left- and right-handed adults: a test-retest functional transcranial Doppler sonography study with six language tasks

A previous study we reported in this journal suggested that left and right-handers may differ in their patterns of lateralization for different language tasks (Woodhead et al. 2019 R. Soc. Open Sci.6, 181801. (doi:10.1098/rsos.181801)). However, it had too few left-handers (N = 7) to reach firm conclusions. For this update paper, further participants were added to the sample to create separate groups of left- (N = 31) and right-handers (N = 43). Two hypotheses were tested: (1) that lateralization would be weaker at the group level in left-than right-handers; and (2) that left-handers would show weaker covariance in lateralization between tasks, supporting a two-factor model. All participants performed the same protocol as in our previous paper: lateralization was measured using functional transcranial Doppler sonography during six different language tasks, on two separate testing sessions. The results supported hypothesis 1, with significant differences in laterality between groups for four out of six tasks. For hypothesis 2, structural equation modelling showed that there was stronger evidence for a two-factor model in left than right-handers; furthermore, examination of the factor loadings suggested that the pattern of laterality across tasks may also differ between handedness groups. These results expand on what is known about the differences in laterality between left- and right-handers

Experimental investigation of a coherent quantum measurement of the degree of polarization of a single mode light beam

A novel method for the direct measurement of the degree of polarization is described. It is one of the first practical implementations of a coherent quantum measurement, the projection on the singlet state. Our first results demonstrate the successful operation of the method. However, due to the nonlinear crystals used presently, its application is limited to spectral widths larger than ~8nm.Comment: 23 pages, 9 figures, submitted to Journal of Modern Optic

"Assisted cloning'' and "orthogonal-complementing" of an unknown state

We propose a protocol where one can exploit dual quantum and classical channels to achieve perfect ``cloning'' and ``orthogonal-complementing'' of an unknown state with a minimal assistance from a state preparer (without revealing what the input state is). The first stage of the protocol requires usual teleportation and in the second stage, the preparer disentangles the left-over entangled states by a single particle measurement process and communicates a number of classical bits (1-cbit per copy) to different parties so that perfect copies and complement copies are produced. We discuss our protocol for producing two copies and three copies (and complement copies) using two and four particle entangled state and suggest how to generalise this for N copies and complement copies using multiparticle entangled state.Comment: 7 pages, Latex, no figures, submitted to Phys. Rev. A. 1999(to be accepted

Conical Indentation of a Viscoelastic Sphere

Instrumented indentation is commonly used for determining mechanical properties of a range of materials, including viscoelastic materials. However, most—if not all—studies are limited to a flat substrate being indented by various shaped indenters (e.g., conical or spherical). This work investigates the possibility of extending instrumented indentation to nonflat viscoelastic substrates. In particular, conical indentation of a sphere is investigated where a semi-analytical approach based on “the method of functional equations” has been developed to obtain the force–displacement relationship. To verify the accuracy of the proposed methodology selected numerical experiments have been performed and good agreement was obtained. Since it takes significantly less time to obtain force–displacement relationships using the proposed method compared to conducting full finite element simulations, the proposed method is an efficient substitute of the finite element method in determining material properties of viscoelatic spherical particles using indentation testing