Translation termination depends on the sequential ribosomal entry of eRF1 and eRF3.

Translation termination requires eRF1 and eRF3 for polypeptide-and tRNA-release on stop codons. Additionally, Dbp5/DDX19 and Rli1/ABCE1 are required; however, their function in this process is currently unknown. Using a combination of in vivo and in vitro experiments, we show that they regulate a stepwise assembly of the termination complex. Rli1 and eRF3-GDP associate with the ribosome first. Subsequently, Dbp5-ATP delivers eRF1 to the stop codon and in this way prevents a premature access of eRF3. Dbp5 dissociates upon placing eRF1 through ATP-hydrolysis. This in turn enables eRF1 to contact eRF3, as the binding of Dbp5 and eRF3 to eRF1 is mutually exclusive. Defects in the Dbp5-guided eRF1 delivery lead to premature contact and premature dissociation of eRF1 and eRF3 from the ribosome and to subsequent stop codon readthrough. Thus, the stepwise Dbp5-controlled termination complex assembly is essential for regular translation termination events. Our data furthermore suggest a possible role of Dbp5/DDX19 in alternative translation termination events, such as during stress response or in developmental processes, which classifies the helicase as a potential drug target for nonsense suppression therapy to treat cancer and neurodegenerative diseases

Coffee Production in Kavre and Lalitpur Districts, Nepal

Coffee (Coffea spp) is an important and emerging cash crop having potential to provide farmers employment and income generation opportunities. This crop is well adapted to the climatic conditions of mid-hills of Nepal. Thus, majority of the farmers are attracted towards cultivation of coffee because of demands in national and International market. Coffee is now becoming integral part of farming system in rural areas. However, information on performance of coffee and farmers response has not been well documented. Therefore, we undertook the present work to analyze demography, ethnicity, household occupation, literacy status, average land holding, coffee cultivation area, livelihood and sources of income of coffee growers, production and productivity, pricing, cropping pattern of the coffee and problesm faced by them in mid hill district of Kavrepalanchowk (hereafter ‘Kavre') and Lalitpur Districts. All the samples were taken randomly and selected from coffee producing cooperative of Kavre and Lalitpur. Our analysis showed that the male farmer dominant over female on adopting coffee cultivation in both districts with higher value in Kavre. Brahmin and Chetri ethnic communities were in majority over others in adopting the coffee cultivation. Literate farmers were more dominant over illiterates on adopting the coffee cultivation, The mean land holding was less, ranging from 0.15 to 2.30 ha for coffee cultivation, the history of coffee cultivation in Kavre showed that highest number of farmers were engaged in coffee farming from last 16 years. The mean yield of fresh cherry was 1027.20 kg/ha in Kavre, while it was 1849.36 kg/ha in Lalitpur. The study revealed that majority of the coffee plantations were between 6-10 years old. The major problems facing by coffee farmers were diseases spread, lack of irrigation facility and drying of plants. Despite of that the coffee farming was one of the rapidly emerging occupations among the farmers in both district of Nepal

Spin-Orbit Scattering and Quantum Metallicity in Ultra-Thin Be Films

We compare and contrast the low temperature magnetotransport properties of ultra-thin, insulating, Be films with and without spin-orbit scattering (SOS). Beryllium films have very little intrinsic SOS, but by "dusting" them with sub-monolayer coverages of Au, one can introduce a well controlled SOS rate. Pure Be films with sheet resistance R >R_Q exhibit a low-temperature negative magnetoresistance (MR) that saturates to the quantum resistance R_Q = h/e^2. This high-field quantum metal phase is believed to represent a new ground state of the system. In contrast, the corresponding negative MR in Be/Au films is greatly diminished, suggesting that, in the presence of strong SOS, the quantum metal phase can only be reached at field scales well beyond those typically available in a low temperature laboratory.Comment: 12 pages, 4 figures, to appear in PR

Effect of Heat Stress on Crossbred Dairy Cattle in Tropical Nepal: Impact on Blood Parameters

Chitwan district of Nepal has been known as the dairy kingdom of the country considering its strategic location andpotential to easily provision inputs and produce as well as market quality milk across the country. However, theclimate especially during summer has been a challenge to farmers that potentially compromise the daily milk yieldof cows. Therefore, sixteen crossbred dairy cattle were placed in a RCBD fashion to assess the impacts of cold waterbathing in ameliorating effect of heat stress on their performance and body physiology. Routine assessment of themicroclimate within the experimental shed and blood parameters was made. The results from the experimentinferred that frequency of bathing (none, once, twice or thrice a day) did not have any significant impact onHaemoglobin, Haematocrit, Sodium, Potassium, Chloride and Bicarbonate levels in the blood plasma(p>0.05). Inaddition, the animals did not exhibit any signs of physiological distress clinically either. A detailed study scopingtemperature humidity index and over a number of other milk and blood parameters are to be tested across a numberof other available breeds too in order for the researchers to come to a meaningful strategy to beat the heat stress

NaV2O4: a Quasi-1D Metallic Antiferromagnet with Half-Metallic Chains

NaV2O4 crystals were grown under high pressure using a NaCl flux, and the crystals were characterized with X-ray diffraction, electrical resistivity, heat capacity, and magnetization. The structure of NaV2O4 consists of double chains of edge-sharing VO6 octahedra. The resistivity is highly anisotropic, with the resistivity perpendicular to the chains more than 20 times greater than that parallel to the chains. Magnetically, the intrachain interactions are ferromagnetic and the interchain interactions are antiferromagnetic; 3D antiferromagnetic order is established at 140 K. First principles electronic structure calculations indicate that the chains are half metallic. Interestingly, the case of NaV2O4 seems to be a quasi-1D analogue of what was found for half-metallic materials.Comment: 14 pages, including 4 figures and 1 table, accepted for publication in PR

Participatory policy analysis in health policy and systems research: reflections from a study in Nepal.

Background Participatory policy analysis (PPA) as a method in health policy and system research remains underexplored. Using our experiences of conducting PPA workshops in Nepal to explore the impact of the country’s move to federalism on its health system, we reflect on the method’s strengths and challenges. We provide an account of the study context, the design and implementation of the workshops, and our reflections on the approach’s strengths and challenges. Findings on the impact of federalism on the health system are beyond the scope of this manuscript. Main body We conducted PPA workshops with a wide range of health system stakeholders (political, administrative and service-level workforce) at the local and provincial levels in Nepal. The workshops consisted of three activities: river of life, brainstorming and prioritization, and problem-tree analysis. Our experiences show that PPA workshops can be a valuable approach to explore health policy and system issues – especially in a context of widespread systemic change which impacts all stakeholders within the health system. Effective engagement of stakeholders and activities that encourage both individual- and system-level reflections and discussions not only help in generating rich qualitative data, but can also address gaps in participants’ understanding of practical, technical and political aspects of the health system, aid policy dissemination of research findings, and assist in identifying short- and long-term practice and policy issues that need to be addressed for better health system performance and outcomes. Conducting PPA workshops is, however, challenging for a number of reasons, including the influence of gatekeepers and power dynamics between stakeholders/participants. The role and skills of researchers/facilitators in navigating such challenges are vital for success. Although the long-term impact of such workshops needs further research, our study shows the usefulness of PPA workshops for researchers, for participants and for the wider health system. Conclusions PPA workshops can effectively generate and synthesize health policy and system evidence through collaborative engagement of health system stakeholders with varied roles. When designed with careful consideration for context and stakeholders’ needs, it has great potential as a method in health policy and systems research

Simulation of Growth and Yield of Rainfed Maize Under Varied Agronomic Management and Changing Climatic Scenario in Nawalparasi, Nepal

Correction: Figure 3 was corrupted and so the PDF was replaced on 29th December 2016 with the corrected Figure 3.A field experiment and simulation modeling study in combination for different maize cultivars planted at different sowing dates were accomplished at Kawasoti-5, Nawalparasi during spring season of 2013 to assess the impact of climate change scenario as predicted by IPCC in rainfed spring maize by using CSM-CERES-Maize model. Result showed that RML-4/RML-17 produced higher kernel rows/ ear (13.77), kernel per row (30.42) and test weight (244.9 g). Significantly higher grain yield was also found for RML-4/RML-17 (6.03 t/ha) compared to Poshilo makai-1 (4.73 t/ha), Arun-2 (3.55 t/ha) and Local (2.92 t/ha). Earlier sowing date (7th April) actually produced higher kernel/row (27.97), kernel rows/ear (12.89) and 1000 grain weight (230 g). Significantly higher grain yield (5.13t/ha) was obtained in earlier sowing date (7th April). The CSM-CERES-Maize model was calibrated and found well validated with days to anthesis (RMSE= 0.426 day and D-index= 0.998), days to physiological maturity (RMSE=0.674 day and D-index= 0.999), number of grain/m2 at maturity (RMSE= 85.287 grain /m2 and D-index= 0.993), unit weight at maturity (RMSE=0.012 g/kernel and D-index= 0.854) and grain yield (RMSE=54.94 kg/ha and D-index= 1.00). The model was found sensitive to climate change parameters. The sensitivity for various climate change parameter indicated that there was severely decreased trend in simulated rainfed spring maize yield with the increment of maximum and minimum temperature, decrease in solar radiation and decrease carbondioxide concentration. Even 2°C rise in temperature can decrease around 15-20% yield of spring maize and this negative effect was even more pronounced in hybrid than other cultivars.Journal of Maize Research and Development (2015) 1(1):123-133DOI: http://dx.doi.org/10.5281/zenodo.3428