Genetic diversity of the 3ꞌ and 5ꞌ untranslated regions of the HSP70.1 gene between native Turkish and Holstein Friesian cattle breeds

Heat stress proteins are important factors in protecting cells against environmental stress. The HSP70.1 gene is one of the most important members of the heat stress protein family, which is essential for life, production and reproduction. In this study, partial regions of HSP70.1 (3ꞌ and 5ꞌ untranslated regions (UTRs)) were sequenced in six cattle breeds. Blood samples of five native breeds, namely Yerli Kara (YK), Boz ırk (BI), Yerli Güney Sarısı (YGS), Güney Doğu Anadolu Kırmızısı (GAK) and Doğu Anadolu Kırmızısı (DAK) were collected from their native regions and blood samples of the Holstein Friesian (Siyah Alaca (SA)) breed were collected from each of these regions. Totals of 249 and 206 animals were analysed for the HSP70.1- 3ꞌ and 5ꞌ UTR regions, respectively. In the 3ꞌ UTR region, 13 single nucleotide polymorphisms (SNPs) and one indel were found, whereas this region was found to be monomorphic among animals of the Holstein Friesian breed. In the 5ꞌ UTR region, 43 SNPs and three indels were revealed in all of the investigated breeds. On the other hand, a new C983T nucleotide substitution was identified in this region, and is thought to disrupt the Sp1-hsp70 promoter binding site. The 5ꞌ UTR region was also more variable in the Turkish native breeds than in the Holstein Friesian. This study is the first to investigate the 3ꞌ and 5ꞌ UTRs of the HSP70.1 gene in Turkish native breeds. The genetic structure of these gene regions in Turkish native cattle breeds was found to be quite different from those of other cattle breeds that had been studied in the past.Keywords: Bovine, heat shock genes, heat stress, polymorphis

On generalized statistical convergence of order β of sequences of fuzzy numbers

In this article we introduce the concepts of Delta(m)(lambda)-statistical convergence of order beta and strong Delta(m)(lambda p)-summability of order beta for sequences of fuzzy numbers. Also, we establish some relations between the Delta(m)(lambda)-statistical convergence of order beta and strong Delta(m)(lambda p)-summability of order beta and we define w(lambda)(beta) (Delta(m), F, f, p), where f is a modulus function and give some inclusion relations

Electrosprayed chitosan-coated alginate-pectin beads as potential system for colon-targeted delivery of ellagic acid

Background: Ellagic acid (EA), a potent dietary antioxidant, has limited bioavailability owing to its rapid absorption in the stomach and small intestine, and EA is transformed to more bioavailable compounds - urolithins - in the colon. An encapsulation system that sustains the release of EA in the gastrointestinal system and delivers more EA into the colon could improve the oral bioavailability of EA. Electrosprayed EA-loaded alginate-pectin beads were produced and coated with low- (LC) and high-molecular-weight chitosan (HC). The EA release from uncoated and coated beads under simulated gastrointestinal conditions was evaluated. The samples were characterized by particle size, gel strength, scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) analysis. Results: The encapsulation efficiency (EE%) of EA ranged from 49.53% to 69.85% for uncoated beads, which was elevated up to 86.50% by coating, and LC coating provided higher EE%. Pectin addition to alginate and chitosan coating reduced the gel strength and changed the size depending on the molecular weight of chitosan. SEM images of pectin-added beads showed fewer cracks but more wrinkles, and chitosan coating presented more aggregated surfaces. The ionic interaction of alginate-pectin-chitosan and the entrapment of EA were confirmed by FTIR. In the gastric medium, EA release was very low from uncoated beads (15.2-19.8%), and totally restricted by chitosan coating. In the intestinal stage, EA release from LC-coated alginate-pectin beads was only 18%, and it was between 55% and 65% for uncoated or HC-coated counterparts. Conclusion: The LC-coated alginate-pectin beads could be further explored as a potential system for colon-targeted delivery of EA. © 2021 Society of Chemical Industry

National thalassemia Prevention campaign: The talotir project [Ulusal Talasemi önleme Kampanyasi: Talotir Projesi]

[No abstract available

FIGURE 2 in Mitogenome of Xya pfaendleri (Orthoptera: Caelifera): Its comparative description and phylogenetic position within Tridactylidea

FIGURE 2. The chronogram of Tridactylidea, with the bootstrap support of ML (lower cell of the box) and posterior probability of BEAST (upper cell of the box) analyses (ages in mya are shown on the right of each node; the node calibrated by 202.67 ± 40 mya based on Song et al. (2015) is denoted by a black circle; X. japonica-Xj1 refers to MK903575 and X. japonica-Xj2 KC555032 GenBank sequences).Published as part of <i>Yahyaoğlu, Özgül, Doğan, Ceren Tutku, Uluar, Onur, Karakaş, Müşerref Y. & Çiplak, Battal, 2023, Mitogenome of Xya pfaendleri (Orthoptera: Caelifera): Its comparative description and phylogenetic position within Tridactylidea, pp. 576-584 in Zootaxa 5369 (4)</i> on page 581, DOI: 10.11646/zootaxa.5369.4.6, <a href="http://zenodo.org/record/10147150">http://zenodo.org/record/10147150</a&gt