Turner syndrome and associated problems in turkish children: A multicenter study

Objective: Turner syndrome (TS) is a chromosomal disorder caused by complete or partial X chromosome monosomy that manifests various clinical features depending on the karyotype and on the genetic background of affected girls. This study aimed to systematically investigate the key clinical features of TS in relationship to karyotype in a large pediatric Turkish patient population. Methods: Our retrospective study included 842 karyotype-proven TS patients aged 0-18 years who were evaluated in 35 different centers in Turkey in the years 2013-2014. Results: The most common karyotype was 45,X (50.7%), followed by 45,X/46,XX (10.8%), 46,X,i(Xq) (10.1%) and 45,X/46,X,i(Xq) (9.5%). Mean age at diagnosis was 10.2±4.4 years. The most common presenting complaints were short stature and delayed puberty. Among patients diagnosed before age one year, the ratio of karyotype 45,X was significantly higher than that of other karyotype groups. Cardiac defects (bicuspid aortic valve, coarctation of the aorta and aortic stenosi) were the most common congenital anomalies, occurring in 25% of the TS cases. This was followed by urinary system anomalies (horseshoe kidney, double collector duct system and renal rotation) detected in 16.3%. Hashimoto’s thyroiditis was found in 11.1% of patients, gastrointestinal abnormalities in 8.9%, ear nose and throat problems in 22.6%, dermatologic problems in 21.8% and osteoporosis in 15.3%. Learning difficulties and/or psychosocial problems were encountered in 39.1%. Insulin resistance and impaired fasting glucose were detected in 3.4% and 2.2%, respectively. Dyslipidemia prevalence was 11.4%. Conclusion: This comprehensive study systematically evaluated the largest group of karyotype-proven TS girls to date. The karyotype distribution, congenital anomaly and comorbidity profile closely parallel that from other countries and support the need for close medical surveillance of these complex patients throughout their lifespan. © Journal of Clinical Research in Pediatric Endocrinology

Drought Resistance of Warm-season Turfgrasses Grown in Mediterranean Region of Turkey

There is a dearth of information about turfgrass drought resistance and adaptation in the Mediterranean region of Turkey. Turfgrass managers in this region need this information to help them make informed decisions regarding turfgrass selection and management. This research was conducted to assess the drought resistance of bermudagrass (Cynodon dactylon), buffalograss (Buchloe dactyloides), bahiagrass (Paspalum notatum), seashore paspalum (Paspalum vaginatum), zoysiagrass (Zoysia japonica), centipedegrass (Eremochloa ophiuroides), and tall fescue (Lolium arundinaceum) under Mediterranean conditions of Turkey. The study was conducted at two locations, Antalya and Mersin, and was repeated in 2006 and 2007 at both locations. One year after establishment, the turfs were subjected to drought stress for 90 days, which was followed by resumption of irrigation for recovery of the turf. Percentage leaf firing, turfgrass quality, and percent green shoot recovery were recorded. There were inter and intraspecies differences detected for percentage leaf firing and shoot recovery. Bermudagrass, bahiagrass, and buffalograss exhibited superior drought resistance as demonstrated by lower leaf firing and better shoot recovery values when compared with other species studied. Centipedegrass and zoysiagrass demonstrated a high leaf firing and very poor shoot recovery, whereas zoysiagrass and tall fescue were unable to recover from the drought stress in the sandy soil. Results showed that 'SWI-1045' (Contessa (R)) and 'SWI-1044' bermudagrass and 'Cody' buffalograss possessed superior drought resistance with acceptable turfgxass quality up to 30 days under drought stress that can be used for water-efficient turf management under the Mediterranean environment

Polyploidy creates higher diversity among Cynodon accessions as assessed by molecular markers

Developing a better understanding of associations among ploidy level, geographic distribution, and genetic diversity of Cynodon accessions could be beneficial to bermudagrass breeding programs, and would enhance our understanding of the evolutionary biology of this warm season grass species. This study was initiated to: (1) determine ploidy analysis of Cynodon accessions collected from Turkey, (2) investigate associations between ploidy level and diversity, (3) determine whether geographic and ploidy distribution are related to nuclear genome variation, and (4) correlate among four nuclear molecular marker systems for Cynodon accessions' genetic analyses. One hundred and eighty-two Cynodon accessions collected in Turkey from an area south of the Taurus Mountains along the Mediterranean cost and ten known genotypes were genotyped using sequence related amplified polymorphism (SRAP), peroxidase gene polymorphism (POGP), inter-simple sequence repeat (ISSR), and random amplified polymorphic DNA (RAPD). The diploids, triploids, tetraploids, pentaploids, and hexaploids revealed by flow cytometry had a linear present band frequency of 0.36, 0.47, 0.49, 0.52, and 0.54, respectively. Regression analysis explained that quadratic relationship between ploidy level and band frequency was the most explanatory (r = 0.62, P < 0.001). The AMOVA results indicated that 91 and 94% of the total variation resided within ploidy level and provinces, respectively. The UPGMA analysis suggested that commercial bermudagrass cultivars only one-third of the available genetic variation. SRAP, POGP, ISSR, and RAPD markers differed in detecting relationships among the bermudagrass genotypes and rare alleles, suggesting more efficiency of combinatory analysis of molecular marker systems. Elucidating Cynodon accessions' genetic structure can aid to enhance breeding programs and broaden genetic base of commercial cultivars

Establishment and Turf Qualities of Warm-season Turfgrasses in the Mediterranean Region

Warm-season turfgrasses are grown throughout the warm humid, subhumid, and semiarid regions. The objective of this study was to determine the adaptation of six warm-season turfgrass species and several of their cultivars to Mediterranean growing conditions of Turkey by evaluating turfgrass establishment rate, quality, color, and percentage of turfgrass cover. Information of this nature is lacking and would be helpful to turfgrass managers and advisers working in the region. A study was conducted over a 2-year period in two locations of the Mediterranean region of Turkey. The warm-season turfgrass species studied were bermudagrass (Cynodon dactylon), buffalograss (Buchloe dactyloides), zoysiagrass (Zoysia japonica), bahiagrass (Paspalum notatum), seashore paspalum (Paspalum vaginatum), and centipedegrass (Eremochloa ophiurioides). Tall fescue (Festuca arundinacea) was included as a cool-season turfgrass species for comparison. Twenty cultivars belonging to these species were evaluated for their establishment, turfgrass color and quality, spring green-up, and fall color retention. Bermudagrass, bahiagrass, and seashore paspalun established 95% or better coverage at 1095 growing degree days [GDD (5 degrees C base temperature)], buffalograss and centipedegrass at 1436 GDD, and 'Zenith' and 'Companion' Zoysiagrass had 90% and 84% coverage at Antalya after accumulating 2031 GDD. 'Sea Spray' seashore paspalum; 'SWI-1044', 'SWI-1045', 'Princess 77', and 'Riviera' bermudagrass; 'Cody' buffalograss; and 'Zenith' zoysiagrass exhibited acceptable turfgrass quality for 7 months throughout the growing season. 'Argentine' and 'Pensacola' bahiagrass; 'Sea Spray' seashore paspalum; and 'SWI-1044' and 'SWI-1045' bermudagrass extended their growing season by retaining their green color 15 days or longer than the rest of the warm-season cultivars and/or species in the fall. The warm-season species stayed fully dormant throughout January and February. Zoysiagrass and buffalograss cultivars showed early spring green-up compared to the other warm-season species studied. Results from this study support the use of warm-season turfgrass species in this Mediterranean region, especially when heat stress and water limitations exist. Tall fescue did not survive summer heat stress necessitating reseeding in fall