Computational fluid dynamic modeling of 100ml and scaled-down 10ml stirred suspension bioreactors enables prediction of embryonic stem cell characteristics

There is a growing necessity for cell cultivation using bioreactors to translate laboratory based culture protocols into reproducible, scalable, and robust bioprocesses. Stirred suspension bioreactors offer several advantages over planar static cultures, including: reduced labour and operating costs, reduced space requirements, greater cellular homogeneity, and increased cell density per volume [1]. An important consideration when using stirred suspension bioreactors is mechanical stimulation. Fluid shear at the fluid-cell interface triggers cellular responses through mechanotransduction and can modulate stem cell proliferation and differentiation. However, if the shear stress caused by the impeller exceeds the tolerance limit of the cells, it causes cell damage and death, resulting in a lower quality and yield of cells. The shear rate distribution depends on bioreactor geometry, impeller agitation rate, cell density, and cell media viscosity [2]. Current scale-up protocols to predict agitation rates rely on maximum values of hydrodynamic variables, which occur only at the impeller tip. The volume averaged shear stress and maximum shear stress differ greatly, and cells dispersed within the liquid experience different local and global forces. This makes it difficult to predict how cells will respond to changes in bioreactor geometries and sizes. Profiling distributed and average forces in the bioreactor is critical to ensure quality and yield in cell manufacturing. Hydrodynamics, specifically velocities, shear rates, and energy dissipation rates, can be studied using computational fluid dynamic (CFD) modeling. Please click Additional Files below to see the full abstract

Proton Driven Plasma Wakefield Acceleration

Plasma wakefield acceleration, either laser driven or electron-bunch driven, has been demonstrated to hold great potential. However, it is not obvious how to scale these approaches to bring particles up to the TeV regime. In this paper, we discuss the possibility of proton-bunch driven plasma wakefield acceleration, and show that high energy electron beams could potentially be produced in a single accelerating stage.Comment: 13 pages, 4 figure

Overview of Plasma Lens Experiments and Recent Results at SPARC_LAB

Beam injection and extraction from a plasma module is still one of the crucial aspects to solve in order to produce high quality electron beams with a plasma accelerator. Proper matching conditions require to focus the incoming high brightness beam down to few microns size and to capture a high divergent beam at the exit without loss of beam quality. Plasma-based lenses have proven to provide focusing gradients of the order of kT/m with radially symmetric focusing thus promising compact and affordable alternative to permanent magnets in the design of transport lines. In this paper an overview of recent experiments and future perspectives of plasma lenses is reported

Enhancing the accuracy of non-invasive glucose sensing in aqueous solutions using combined millimeter wave and near infrared transmission

We reported measurement results relating to non-invasive glucose sensing using a novel multiwavelength approach that combines radio frequency and near infrared signals in transmission through aqueous glucose-loaded solutions. Data were collected simultaneously in the 37–39 GHz and 900–1800 nm electromagnetic bands. We successfully detected changes in the glucose solutions with varying glucose concentrations between 80 and 5000 mg/dl. The measurements showed for the first time that, compared to single modality systems, greater accuracy on glucose level prediction can be achieved when combining transmission data from these distinct electromagnetic bands, boosted by machine learning algorithms.This research was funded partially by Innovate UK project 104554 (2019–2021)

Climatic Feedbacks and Desertification: The Mediterranean Model

Abstract Mesometeorological information obtained in several research projects in southern Europe has been used to analyze perceived changes in the western Mediterranean summer storm regime. A procedure was developed to disaggregate daily precipitation data into three main components: frontal precipitation, summer storms, and Mediterranean cyclogenesis. Working hypotheses were derived on the likely processes involved. The results indicate that the precipitation regime in this Mediterranean region is very sensitive to variations in surface airmass temperature and moisture. Land-use perturbations that accumulated over historical time and greatly accelerated in the last 30 yr may have induced changes from an open, monsoon-type regime with frequent summer storms over the mountains inland to one dominated by closed vertical recirculations where feedback mechanisms favor the loss of storms over the coastal mountains and additional heating of the sea surface temperature during summer. This, in turn, favors Mediterranean cyclogenesis and torrential rains in autumn–winter. Because these intense rains and floods can occur anywhere in the basin, perturbations to the hydrological cycle in any part of the basin can propagate to the whole basin and adjacent regions. Furthermore, present levels of air pollutants can produce greenhouse heating, amplifying the perturbations and pushing the system over critical threshold levels. The questions raised are relevant for the new European Union (EU) water policies in southern Europe and for other regions dominated by monsoon-type weather systems

A relocatable ocean model in support of environmental emergencies

During the Costa Concordia emergency case, regional, subregional, and relocatable ocean models have been used together with the oil spill model, MEDSLIK-II, to provide ocean currents forecasts, possible oil spill scenarios, and drifters trajectories simulations. The models results together with the evaluation of their performances are presented in this paper. In particular, we focused this work on the implementation of the Interactive Relocatable Nested Ocean Model (IRENOM), based on the Harvard Ocean Prediction System (HOPS), for the Costa Concordia emergency and on its validation using drifters released in the area of the accident. It is shown that thanks to the capability of improving easily and quickly its configuration, the IRENOM results are of greater accuracy than the results achieved using regional or subregional model products. The model topography, and to the initialization procedures, and the horizontal resolution are the key model settings to be configured. Furthermore, the IRENOM currents and the MEDSLIK-II simulated trajectories showed to be sensitive to the spatial resolution of the meteorological fields used, providing higher prediction skills with higher resolution wind forcing.MEDESS4MS Project; TESSA Project; MyOcean2 Projectinfo:eu-repo/semantics/publishedVersio

Airborne Microalgae: Insights, Opportunities and Challenges

Airborne dispersal of microalgae has largely been a blind spot in environmental biological studies because of their low concentration in the atmosphere and the technical limitations in investigating microalgae from air samples. Recent studies show that airborne microalgae can survive air transportation and interact with the environment and possibly influence their deposition rates. This minireview presents a summary of these studies and traces the possible route, step-by-step, from established ecosystems to new habitats through air transportation over a variety of geographic scales. Emission, transportation, deposition and adaptation to atmospheric stress are discussed, as well as the consequences of their dispersal on health and environment, and the state-of-the-art techniques to detect and model airborne microalgae dispersal. More detailed studies on microalgae atmospheric-cycle, including for instance ice nucleation activity and transport simulations, are crucial for improving our understanding of microalgae ecology, identifying their interactions with the environment and preventing unwanted sanitary events or invasions

Perioperative mortality after hemiarthroplasty related to fixation method: A study based on the Australian Orthopaedic Association National Joint Replacement Registry

Background and purpose: The appropriate fixation method for hemiarthroplasty of the hip as it relates to implant survivorship and patient mortality is a matter of ongoing debate. We examined the influence of fixation method on revision rate and mortality.----- ----- Methods: We analyzed approximately 25,000 hemiarthroplasty cases from the AOA National Joint Replacement Registry. Deaths at 1 day, 1 week, 1 month, and 1 year were compared for all patients and among subgroups based on implant type.----- ----- Results: Patients treated with cemented monoblock hemiarthroplasty had a 1.7-times higher day-1 mortality compared to uncemented monoblock components (p < 0.001). This finding was reversed by 1 week, 1 month, and 1 year after surgery (p < 0.001). Modular hemiarthroplasties did not reveal a difference in mortality between fixation methods at any time point.----- ----- Interpretation: This study shows lower (or similar) overall mortality with cemented hemiarthroplasty of the hip

Ontogeny of Toll-Like Receptor Mediated Cytokine Responses of Human Blood Mononuclear Cells

Newborns and young infants suffer increased infectious morbidity and mortality as compared to older children and adults. Morbidity and mortality due to infection are highest during the first weeks of life, decreasing over several years. Furthermore, most vaccines are not administered around birth, but over the first few years of life. A more complete understanding of the ontogeny of the immune system over the first years of life is thus urgently needed. Here, we applied the most comprehensive analysis focused on the innate immune response following TLR stimulation over the first 2 years of life in the largest such longitudinal cohort studied to-date (35 subjects). We found that innate TLR responses (i) known to support Th17 adaptive immune responses (IL-23, IL-6) peaked around birth and declined over the following 2 years only to increase again by adulthood; (ii) potentially supporting antiviral defense (IFN-α) reached adult level function by 1 year of age; (iii) known to support Th1 type immunity (IL-12p70, IFN-γ) slowly rose from a low at birth but remained far below adult responses even at 2 years of age; (iv) inducing IL-10 production steadily declined from a high around birth to adult levels by 1 or 2 years of age, and; (v) leading to production of TNF-α or IL-1β varied by stimuli. Our data contradict the notion of a linear progression from an ‘immature’ neonatal to a ‘mature’ adult pattern, but instead indicate the existence of qualitative and quantitative age-specific changes in innate immune reactivity in response to TLR stimulation