A review of exposure assessment methods for epidemiological studies of health effects related to industrially contaminated sites

BACKGROUND: this paper is based upon work from COST Action ICSHNet. Health risks related to living close to industrially contaminated sites (ICSs) are a public concern. Toxicology-based risk assessment of single contaminants is the main approach to assess health risks, but epidemiological studies which investigate the relationships between exposure and health directly in the affected population have contributed important evidence. Limitations in exposure assessment have substantially contributed to uncertainty about associations found in epidemiological studies. OBJECTIVES: to examine exposure assessment methods that have been used in epidemiological studies on ICSs and to provide recommendations for improved exposure assessment in epidemiological studies by comparing exposure assessment methods in epidemiological studies and risk assessments. METHODS: after defining the multi-media framework of exposure related to ICSs, we discussed selected multi-media models applied in Europe. We provided an overview of exposure assessment in 54 epidemiological studies from a systematic review of hazardous waste sites; a systematic review of 41 epidemiological studies on incinerators and 52 additional studies on ICSs and health identified for this review. RESULTS: we identified 10 multi-media models used in Europe primarily for risk assessment. Recent models incorporated estimation of internal biomarker levels. Predictions of the models differ particularly for the routes ‘indoor air inhalation’ and ‘vegetable consumption’. Virtually all of the 54 hazardous waste studies used proximity indicators of exposure, based on municipality or zip code of residence (28 studies) or distance to a contaminated site (25 studies). One study used human biomonitoring. In virtually all epidemiological studies, actual land use was ignored. In the 52 additional studies on contaminated sites, proximity indicators were applied in 39 studies, air pollution dispersion modelling in 6 studies, and human biomonitoring in 9 studies. Exposure assessment in epidemiological studies on incinerators included indicators (presence of source in municipality and distance to the incinerator) and air dispersion modelling. Environmental multi-media modelling methods were not applied in any of the three groups of studies. CONCLUSIONS: recommendations for refined exposure assessment in epidemiological studies included the use of more sophisticated exposure metrics instead of simple proximity indicators where feasible, as distance from a source results in misclassification of exposure as it ignores key determinants of environmental fate and transport, source characteristics, land use, and human consumption behaviour. More validation studies using personal exposure or human biomonitoring are needed to assess misclassification of exposure. Exposure assessment should take more advantage of the detailed multi-media exposure assessment procedures developed for risk assessment. The use of indicators can be substantially improved by linking definition of zones of exposure to existing knowledge of extent of dispersion. Studies should incorporate more often land use and individual behaviour

Assessment of age-related changes in pediatric gastrointestinal solubility

PurposeCompound solubility serves as a surrogate indicator of oral biopharmaceutical performance. Between infancy and adulthood, marked compositional changes in gastrointestinal (GI) fluids occur. This study serves to assess how developmental changes in GI fluid composition affects compound solubility.MethodsSolubility assessments were conducted in vitro using biorelevant media reflective of age-specific pediatric cohorts (i.e., neonates and infants). Previously published adult media (i.e., FaSSGF, FeSSGF, FaSSIF.v2, and FeSSIF.v2) were employed as references for pediatric media development. Investigations assessing age-specific changes in GI fluid parameters (i.e., pepsin, bile acids, pH, osmolality, etc.) were collected from the literature and served to define the composition of neonatal and infant media. Solubility assessments at 37°C were conducted for seven BCS Class II compounds within the developed pediatric and reference adult media.ResultsFor six of the seven compounds investigated, solubility fell outside an 80–125% range from adult values in at least one of the developed pediatric media. This result indicates a potential for age-related alterations in oral drug performance, especially for compounds whose absorption is delimited by solubility (i.e., BCS Class II).ConclusionDevelopmental changes in GI fluid composition can result in relevant discrepancies in luminal compound solubility between children and adults.<br/

Exposure to phthalates from personal care products: Urinary levels and predictors of exposure

Phthalates are a large group of chemicals used in many everyday consumer products such as food packaging, household cleaners, cosmetics, fragrances and personal care products (PCPs). A number of diseases such as obesity, hypertension, as well as reproductive system effects and endocrine disorders have been linked to phthalate exposure through the use of PCPs, due to their frequent use and high phthalate content. In this study we review available literature on phthalates and their metabolites in urine and report the various determinants of exposure through the use of PCPs in infants, toddlers, children and adults. The range of creatinine-adjusted concentrations for each phthalate was 1.5–14956.1 μg/g for MEP, 0.4–94.5 μg/g for MEHP, 0.39–425.9 μg/g for MEHHP, 0.5–481.3 μg/g for MEOHP, 0.1–755.1 μg/g for MBzP, and 0.3–401.4 μg/g for MiBP. Time of sampling, frequency of use, race and age are critical factors that influence phthalate concentrations. Using PCPs 48 h before urine collection, using a combination of PCPs (i.e., particularly leave-on versus rinse-off products), being younger (i.e., children compared to their mothers), and being a woman of colour (i.e., Mexican-American and black versus white) leads to higher phthalate levels in urine. The most striking association between any phthalate and PCPs was observed between MEP and perfumes or fragrance-containing products such as shampoos, body lotions and hair products. Future studies should focus on different types and brands of PCPs (i.e., branded versus generic), explore possible ethnic/racial differences and the applicability of non-invasive matrices such as nails and hair for phthalate biomonitoring, as well as intervention studies that explore behavioural changes. © 2022 Elsevier Inc

Polybrominated diphenyl ethers (PBDEs) in grey seals during their first year of life – are they thyroid hormone endocrine disrupters?

Blubber biopsy and blood samples were collected from weaned grey seal pups and juveniles in 1998 and 1999. The relationship between the concentration of total PDBEs in the blubber and the circulating concentrations of serum proteins, minerals and thyroid hormones was investigated. Fifty-four post-weaned pups were sampled and 55 first year juveniles, of which 13 were recaptured post-weaned pups. The median concentration of total PBDEs in the pups’ blubber was 170 and 460 ng g−1 in the first-year animals. Total blubber PBDEs contributed significantly to the prediction of circulating thyroid hormone concentrations, after controlling for age and condition. Cholesterol and albumin concentrations were also positively related to blubber PBDEs. The results of this study suggest a link between thyroid hormones and exposure to PBDEs in grey seals during their first year of life. Such an association is not alone sufficient evidence for a cause and effect relationship, however it is in accordance with the hypothesis that these compounds are endocrine disrupters in grey seal pups

The occurrence and accumulation of PBDEs and selected organochlorines in tagged grey seal (Halichoerus grypus) pups in the North Sea.

Blubber samples from grey seal (Halichoerus grypus) pups were collected in 1998 through to 2000. Twenty four newly weaned pups were sampled in November 1998, 13 of which were re-sampled during their first year of life, between March and December 1999, and an additional 48 and 25 samples (new cohorts) were obtained in 1999 (November–December) and 2000 (May–September) respectively. ∑PBDE concentrations (sum of Di-HxBDEs) ranged from 45 to 1500 ng/g lipid, with a geometric mean of 290 ng/g lipid. BDE 47 dominated the congener profile, followed by BDEs 100, 99, 153 and 154. ∑PCB concentrations (sum of 41 congeners) ranged from 100 to 93 000 ng/g lipid, with a geometric mean of 4600 ng/g lipid, with CB congeners 153, 138 and 180 making up 84% of the total PCB concentrations. The predominant OC pesticides were p,p′-DDE (geometric mean = 860 ng/g lipid, range = 160–3800 ng/g lipid) and p,p′-DDT (geometric mean = 120 ng/g lipid, range = 30–670 ng/g lipid). No significant differences were found between males and females, probably due to the young age of the animals. Significant decreases (P < 0.01 for PBDEs and P < 0.05 for PCBs/OCs) were observed between newly weaned seals in 1998 and 1999, but not for the majority of juveniles in 1999 and 2000. Significant differences (P < 0.01) were also found between the different seasons in 1999 newly weaned and juvenile seals, reflecting changes in blubber mass

Evaluation of Organic Environmental Pollutants Detected in Human Milk

Human milk is an important food source for infant because it contains a large number of nutritional substances, growth and immune factors. However, human milk may be contaminated with environmental pollutants when mothers are exposed to these pollutants. In particular, lipophilic organic pollutants are likely to accumulate in milk fat. Therefore, the determination of the organic pollutants levels in human milk is necessary to estimate the health risks of these pollutants to milk-fed infants. For this purpose, a lot of reports for the measurements of environmental pollutants in milk samples have been published. In this review, we summarized the concentrations of harmful organic environmental pollutants such as polychlorinated organic compounds (PCOCs), polybrominated compounds, perfluorinated compounds (PFCs), polycyclic aromatic hydrocarbons (PAHs) and endocrine disrupting phenols in human milk samples. Also, we described the noteworthy results of several evaluative studies such as time trend and regional difference of pollutant levels

Engaging the senses: The association of urban green space with general health and well-being in urban residents

This study evaluated the short-term responses of physiological and psychological indices and examined the human senses that are mostly engaged during a green space and urban exposure in residents of Athens, Greece. The forest had beneficial effects for human physiology, anxiety and mood states and was also associated with all five senses and positive reactions, while the opposite was observed in the urban center. The difference of pre-and post-green space exposure salivary cortisol was correlated with the participants’ environmental profile and body mass index. Green spaces can alleviate stress and improve overall mood, while helping individuals experience their surroundings with all five senses. © 2021 by the authors. Licensee MDPI, Basel, Switzerland