X-ray structure of Galdieria Rubisco complexed with one sulfate ion per active site

AbstractRibulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyzes the reactions of carboxylation and oxygenation of ribulose-1,5-bisphosphate. These reactions require that the active site should be closed by a flexible loop (loop 6) of the large subunit. Rubisco from a red alga, Galdieria partita, has the highest specificity for carboxylation reaction among the Rubiscos hitherto reported. The crystal structure of unactivated Galdieria Rubisco has been determined at 2.6 Å resolution. The electron density map reveals that a sulfate binds only to the P1 anion-binding site of the active site and the loop 6 is closed. Galdieria Rubisco has a unique hydrogen bond between the main chain oxygen of Val332 on the loop 6 and the ϵ-amino group of Gln386 of the same large subunit. This interaction is likely to be crucial to understanding for stabilizing the loop 6 in the closed state and to making a higher affinity for anionic ligands

低速電子線励起用新規橙赤色硫化物蛍光体の開発

電子線によって励起されうる新規硫化物蛍光体の開発を行っている．真空下または硫黄蒸気下の両方で，固相反応による試料作製を行った．La2S3:Ce3+ にて赤色, Y4(SiS4)3:Ce3+ にて橙色の発光を得ることに成功した．La2S3 では0.1 から20 mol% の範囲でCe3+ 濃度を変化させることにより，1 mol% が最適であることがわかった．蛍光表示管での実装評価を行い，赤色および橙色のカソードルミネッセンスを得た．低速の電子線で励起されるためには蛍光体粒子の最表面に位置するCe3+ が発光する必要がある．電子線励起の際の加速電圧により，電子の侵入長が変化する．試料作製時の雰囲気により，カソードルミネッセンス強度の加速電圧依存性が異なった．New red or orange sulfide phosphors are studied. La2S3:Ce3+ and Y4(SiS4)3:Ce3+ were synthesized by solid-state reaction under vacuum or sulfur vapor atmosphere. Red or orange photoluminescence and cathodeluminescence were obtained from La2S3:Ce3+ or Y4(SiS4)3:Ce3+, respectively. Test devices of vacuum fluorescence display were prepared, and cathodeluminescence intensity was unfortunately as small as 10 cd m-2 at present. The atmosphere in the solid-state reaction of the phosphor synthesis changed the dependence of cathodeluminescence intensity on acceleration voltage. Ce3+ ions on the surface region in the phosphor powder are believed to be important for efficient cathodeluminescence

Phylogenetic analysis of the long terminal repeat of feline immunodeficiency viruses from Japan, Argentina and Australia

The nucleotide sequences of the long terminal repeat of five Japanese, five Argentine and three Australian isolates of feline immunodeficiency virus (FIV) were determined and compared with those of isolates previously described. The results revealed that the Japanese isolates were found to cluster with nucleotide sequence similarity of 95.6%–99.4%. The Australian isolates also clustered with nucleotide sequence similarity of 97.2%–99.4%. The Argentine isolates formed two groups; the LP9 isolate is closely related to the Japanese isolates, whereas the LP1, LP3, LP20 and LP24 isolates are distant from both the Japanese and Australian isolates. From these results, FIV can be divided into three groups, namely: (I) the Californian, Australian and British isolates; (II) the Japanese isolates and one Argentine LP9 isolate; (III) the other Argentine isolates.Facultad de Ciencias Veterinaria

Detection of Transgenes in Gene Delivery Model Mice by Adenoviral Vector Using ddPCR

With the rapid progress of genetic engineering and gene therapy, the World Anti-Doping Agency has been alerted to gene doping and prohibited its use in sports. However, there is no standard method available yet for the detection of transgenes delivered by recombinant adenoviral (rAdV) vectors. Here, we aim to develop a detection method for transgenes delivered by rAdV vectors in a mouse model that mimics gene doping. These rAdV vectors containing the mCherry gene was delivered in mice through intravenous injection or local muscular injection. After five days, stool and whole blood samples were collected, and total DNA was extracted. As additional experiments, whole blood was also collected from the mouse tail tip until 15 days from injection of the rAdv vector. Transgene fragments from different DNA samples were analyzed using semi-quantitative PCR (sqPCR), quantitative PCR (qPCR), and droplet digital PCR (ddPCR). In the results, transgene fragments could be directly detected from blood cell fraction DNA, plasma cell-free DNA, and stool DNA by qPCR and ddPCR, depending on specimen type and injection methods. We observed that a combination of blood cell fraction DNA and ddPCR was more sensitive than other combinations used in this model. These results could accelerate the development of detection methods for gene doping