334 research outputs found
Dynamic Anchoring of PKA Is Essential during Oocyte Maturation
SummaryIn the final stages of ovarian follicular development, the mouse oocyte remains arrested in the first meiotic prophase, and cAMP-stimulated PKA plays an essential role in this arrest. After the LH surge, a decrease in cAMP and PKA activity in the oocyte initiates an irreversible maturation process that culminates in a second arrest at metaphase II prior to fertilization [1]. A-kinase anchoring proteins (AKAPs) mediate the intracellular localization of PKA and control the specificity and kinetics of substrate phosphorylation [2]. Several AKAPs have been identified in oocytes including one at 140 kDa [3, 4] that we now identify as a product of the Akap1 gene. We show that PKA interaction with AKAPs is essential for two sequential steps in the maturation process: the initial maintenance of meiotic arrest and the subsequent irreversible progression to the polar body extruded stage. A peptide inhibitor (HT31) that disrupts AKAP/PKA interactions stimulates oocyte maturation in the continued presence of high cAMP. However, during the early minutes of maturation, type II PKA moves from cytoplasmic sites to the mitochondria, where it associates with AKAP1, and this is shown to be essential for maturation to continue irreversibly
First Case Start Times for Vascular Surgery
Problem/Impact Statement:
85% of first cases at Maine Medical Center for Vascular Surgery start late. According to one study done by Aurora Health Care; of 5,500 first case surgeries, 88% of them started late. The impact of this is far reaching. It is not in alignment with MMC value of Patient Centered Care because the patient becomes dissatisfied waiting to be brought in to surgery , they are fasting for longer than anticipated, and being away from their family while they wait causing anxiety. The financial impact is $1995 for each 1â2 hr. of O.R. time. Furthermore, this may result in elective cases being canceled, late cases create a back log of cases to be done, the hospital loses potential revenue, and staff stay later causing overtime accrual
âLike, pissing yourself is not a particularly attractive quality, letâs be honestâ : learning to contain through youth, adulthood, disability and sexuality
In this article, we (re)conceptualise containment in the context of youth, gender, disability, crip sex/uality and pleasure. We begin by exploring eugenic histories of containment and trace the ways in which the anomalous embodiment of disabled people (Shildrick, 2009) remains vigorously policed within current neo-eugenic discourse. Drawing upon data from two corresponding research studies, we bring the lived experiences of disabled young people to the fore. We explore their stories of performing, enacting and realising containment: containing the posited unruliness of the leaky impaired body; containment as a form of (gendered) labour (Liddiard, 2013a); containment as a marker of normalisation and sexualisation, and thus a necessary component for ableist adulthood (Slater, 2015). Thus, we theorise crip embodiment as permeable, porous and thus problematic in the context of the impossibly bound compulsory (sexually) able adult body (McRuer, 2006). We suggest that the implicit learning of containment is therefore required of disabled young people, particularly women, to counter infantilising and desexualising discourse and cross the 'border zone of youth' (Lesko, 2012) and achieve normative neoliberal adulthood. Crucially, however, we examine the meaning of what we argue are important moments of messiness: the precarious localities of leakage which disrupt containment and thus the 'reality' of the 'able' 'adult' body. We conclude by considering the ways in which these bodily ways of being contour both material experiences of pleasure and the right(s) to obtain it
Comparative ABA‑responsive transcriptome in soybean cultivars submitted to different levels of drought.
First online
Screening de proteĂnas bioativas com potencial antioxidante em proteoma de soja cv. BRS 537.
School toilets : queer, disabled bodies and gendered lessons of embodiment
In this paper we argue that school toilets function as one civilising site (Elias, 1978) in which children learn that disabled and queer bodies are out of place. This paper is the first to offer queer and crip perspectives on school toilets. The small body of existing school toilet literature generally works from a normative position which implicitly perpetuates dominant and oppressive ideals. We draw on data from Around the Toilet, a collaborative research project with queer, trans and disabled people (aroundthetoilet.wordpress.com) to critically interrogate this work. In doing this we consider âtoilet trainingâ as a form of âcivilisationâ, that teaches lessons around identity, embodiment and ab/normal ways of being in the world. Furthermore, we show that âtoilet trainingâ continues into adulthood, albeit in ways that are less easily identifiable than in the early years. We therefore call for a more critical, inclusive, and transformative approach to school toilet research
Search for right-handed W bosons in top quark decay
We present a measurement of the fraction f+ of right-handed W bosons produced
in top quark decays, based on a candidate sample of events in the
lepton+jets decay mode. These data correspond to an integrated luminosity of
230pb^-1, collected by the DO detector at the Fermilab Tevatron
Collider at sqrt(s)=1.96 TeV. We use a constrained fit to reconstruct the
kinematics of the and decay products, which allows for the
measurement of the leptonic decay angle for each event. By comparing
the distribution from the data with those for the expected
background and signal for various values of f+, we find
f+=0.00+-0.13(stat)+-0.07(syst). This measurement is consistent with the
standard model prediction of f+=3.6x10^-4.Comment: Submitted to Physical Review D Rapid Communications 7 pages, 3
figure
Measurement of the Lifetime Difference in the B_s^0 System
We present a study of the decay B_s^0 -> J/psi phi We obtain the CP-odd
fraction in the final state at time zero, R_perp = 0.16 +/- 0.10 (stat) +/-
0.02 (syst), the average lifetime of the (B_s, B_sbar) system, tau (B_s^0)
=1.39^{+0.13}_{-0.16} (stat) ^{+0.01}_{-0.02} (syst) ps, and the relative width
difference between the heavy and light mass eigenstates, Delta Gamma/Gamma =
(Gamma_L - Gamma_H)/Gamma =0.24^{+0.28}_{-0.38} (stat) ^{+0.03}_{-0.04} (syst).
With the additional constraint from the world average of the B_s^0$lifetime
measurements using semileptonic decays, we find tau (B_s^0)= 1.39 +/- 0.06 ~ps
and Delta Gamma/\Gamma = 0.25^{+0.14}_{-0.15}. For the ratio of the B_s^0 and
B^0 lifetimes we obtain tau(B_s^0)/tau(B^0)} = 0.91 +/- 0.09 (stat) +/- 0.003
(syst).Comment: submitted to Phys. Rev. Lett. FERMILAB-PUB-05-324-
A search for W bb and W Higgs production in ppbar collisions at sqrt(s)=1.96 TeV
We present a search for W b \bar{b} production in p \bar{p} collisions at
sqrt{s}=1.96 TeV in events containing one electron, an imbalance in transverse
momentum, and two b-tagged jets. Using 174 pb-1 of integrated luminosity
accumulated by the D0 experiment at the Fermilab Tevatron collider, and the
standard-model description of such events, we set a 95% C.L. upper limit on W b
\bar{b}WH--$135
GeV.Comment: 7 pages, 4 figures, 1 table, submitted to Physical Review Letter
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