Effect of organic manures on growth, yield and quality of turmeric (Curcuma longa L)

The use of organic manures in the agricultural plays a vital role in improving the quality of turmeric as well as improving the soil health. Turmeric is having a close relation with human health; hence demand of organically grown turmeric is increasing tremendously. A field trial was conducted to study the effect of different organic manures (generally recommended fertilizer dose, farmyard manure, vermicompost, press mud compost, poultry manure, sugarcane trash, wheat straw, turmeric trash and jeevamrut ) on the yield and curcumin content of turmeric Cv. Phule Swarupa on Inceptisol at Agricultural Research Station, Dist-Sangli, Maharashtra, India. The trial was conducted on the fixed site of soil for the continuous of five years (2009-2013). The maximum dry yield (62.42 q ha-1) of the turmeric was recorded by application of the general recommended dose of fertilizer (GRDF) i. e. 25 MT FYM + 200:100:100 kg N:P2O5:K2O ha-1 which was at par with the vermicompost (11.36 T ha-1). The highest number of leaves per tiller and number of tillers per plant, plant height and curcumin content was recorded in the GRDF, which was at par with vermicompost. The highest benefit: cost ratio (1.59) was also noticed in GRDF, which was at par with the application of vermicompost (1.54) on a nitrogen basis. The soil available nutrient status was taken into consideration during experimentation. The application of vermicompost (11.36 T ha-1) along with Phosphate Solubilizing Bacteria and Azospirillium @ 5 kg ha-1, respectively at the time of planting was found superior for higher dry yield (55.45 q ha-1), net monetary returns (Rs. 137035) and maintenance of soil fertility for organic cultivation of turmeric

The missing gonadotropin-releasing hormone agonist trigger leading to missing oocytes

Authors present a case of Genuine EFS in a young woman where no oocytes were retrieved in the first stimulation cycle after single ovulation trigger. However, a repeat stimulation performed with double ovulation trigger yielded enough oocytes

Системный анализ эффективности функционирования электрических машин в горнодобывающем комплексе

Представлена методология, позволяющая оценить и обеспечить эффективность функционирования электрических машин с точки зрения системного анализа. Произведено дезагрегирование цели на составные элементы, которые отражены в виде дерева целей, что позволяет изучить структуру обеспечения эффективности эксплуатации и обслуживания электрических машин. На основе экспертной оценки определены коэффициенты относительной важности компонентов дерева целей. Методология может быть использована для выявления и структуризации проблем, связанных с эксплуатацией и обслуживанием электрических машин

Effect of different shading intensities on growth and yield of cherry tomato

Cherry tomato (Solanum lycopersicon var cerasiforme) is small size fruits, with a bright red colour resembling to cherry and becoming popular in the retail chains which are marketed at a premium price. The field experiment was conducted to study the effect of different (35, 50 and 75 per cent) shading intensities on growth and yield (qha-1 ) of cherry tomato. Significantly maximum yield was recorded in 35 per cent shading intensity and genotype Kalash Seeds Product (KSP)-113 (579.44 and 503.88 q ha-1 , respectively). Among the different shading intensities and genotypes, maximum plant height was observed in 75 per cent shading intensity and genotype KSP -113 at 30 days interval (74.70 and 60.95 cm, respectively). The minimum days to 50% flowering of cherry tomato were observed in cherry tomatoes grown under 35 per cent shading intensity (45.00 days) as compared to other shading intensities while minimum days to 50% flowering were observed in genotype KSP-113 (44.00 days). The maximum length of the cluster (9.58 cm), the weight of cluster (27.67 g), number of fruits per cluster (9.42) and number of pickings (11.67) were observed in 35 per cent shading intensities and in genotype KSP-113. The cultivation of KSP-113 genotype under 35 per cent shading intensity was found to be most sustainable for improving growth and yield of cherry tomato during the summer season

Isolation of L-asparginase from marine bacterium Bacillus subtilis and its characterization

Microbial L-asparginases has wide range of applications as therapeutic agents and in industries. In the present study, 57 bacterial isolates from Konark beach, Bhubaneshwar were screened for L-asparginase production and KBI-13 isolate was found to be potential producer strain. KBI-13 was identified as Bacillus subtilis at molecular levels. During production optimization, pH (8.0), temperature (40 ºC), carbon and nitrogen sources (dextrose- 0.5 %; yeast extract 1 %), aeration conditions, metal salts (FeSO4) and NaCl (4 %) were found to be optimum. The enzyme was produced under optimized conditions and was purified by sephadex G-50 column and the purification was obtained upto 61.54 fold. The activity of enzyme was increased upto pH 8.0 and temperature 40 ºC and its stability was observed upto 16 hrs at 40 ºC temperature and pH 8.0. Pretreatment of 0.5 mM CaCl2 increased the enzyme activity upto 20 % while, 250 mM concentration of L-aspargine was suitable for optimum activity of enzyme which was further confirmed by values of Vmax (1.25 µM/min) and Km (0.05 mM). The reaction end products did not show any significant change in enzyme activity

Isolation of L-asparginase from marine bacterium Bacillus subtilis and its characterization

97-106Microbial L-asparginases has wide range of applications as therapeutic agents and in industries. In the present study, 57 bacterial isolates from Konark beach, Bhubaneshwar were screened for L-asparginase production and KBI-13 isolate was found to be potential producer strain. KBI-13 was identified as Bacillus subtilis at molecular levels. During production optimization, pH (8.0), temperature (40 ºC), carbon and nitrogen sources (dextrose- 0.5 %; yeast extract 1 %), aeration conditions, metal salts (FeSO4) and NaCl (4 %) were found to be optimum. The enzyme was produced under optimized conditions and was purified by sephadex G-50 column and the purification was obtained upto 61.54 fold. The activity of enzyme was increased upto pH 8.0 and temperature 40 ºC and its stability was observed upto 16 hrs at 40 ºC temperature and pH 8.0. Pretreatment of 0.5 mM CaCl2 increased the enzyme activity upto 20 % while, 250 mM concentration of L-aspargine was suitable for optimum activity of enzyme which was further confirmed by values of Vmax (1.25 µM/min) and Km (0.05 mM). The reaction end products did not show any significant change in enzyme activity. 

Science with an ngVLA: Resolving the Radio Complexity of EXor and FUor-type Systems with the ngVLA

Episodic accretion may be a common occurrence in the evolution of young pre-main sequence stars and has important implications for our understanding of star and planet formation. Many fundamental aspects of what drives the accretion physics, however, are still unknown. The ngVLA will be a key tool in understanding the nature of these events. The high spatial resolution, broad spectral coverage, and unprecedented sensitivity will allow for the detailed analysis of outburst systems. The proposed frequency range of the ngVLA allows for observations of the gas, dust, and non-thermal emission from the star and disk.Comment: 8 pages, 1 figure, To be published in the ASP Monograph Series, "Science with a Next-Generation VLA", ed. E. J. Murphy (ASP, San Francisco, CA

Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues

Hormones and nutrients often induce genetic programs via signaling pathways that interface with gene-specific activators. Activation of the cAMP pathway, for example, stimulates cellular gene expression by means of the PKA-mediated phosphorylation of cAMP-response element binding protein (CREB) at Ser-133. Here, we use genome-wide approaches to characterize target genes that are regulated by CREB in different cellular contexts. CREB was found to occupy approximate to 4,000 promoter sites in vivo, depending on the presence and methylation state of consensus cAMP response elements near the promoter. The profiles for CREB occupancy were very similar in different human tissues, and exposure to a cAMP agonist stimulated CREB phosphorylation over a majority of these sites. Only a small proportion of CREB target genes was induced by cAMP in any cell type, however, due in part to the preferential recruitment of the coactivator CREB-binding protein to those promoters. These results indicate that CREB phosphorylation alone is not a reliable predictor of target gene activation and that additional CREB regulatory partners are required for recruitment of the transcriptional apparatus to the promoter

Advances in chemical and biological methods to identify microorganisms—from past to present

Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cost grouping of bacterial/fungal isolates and current analysis call for broad reports of microorganisms, involving the application of molecular techniques (e.g., 16S ribosomal RNA gene sequencing based on polymerase chain reaction). The goal of this review is to present the past and the present methods of detection and identification of microorganisms, and to discuss their advantages and their limitations.C.F.R. would like to thank the Portuguese Foundation for Science and Technology (FCT–Portugal) for the C.F.R. for the project UID/EQU/00511/2019—Laboratory for Process Engineering, Environment, Biotechnology, and Energy—LEPABE funded by national funds through FCT/MCTES (PIDDAC) and N.M. for the Strategic project ref. UID/BIM/04293/2013 and “NORTE2020 - Programa Operacional Regional do Norte” (NORTE-01-0145-FEDER-000012)