Integrated dataset on acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin

Data herein describe the quantitative changes in the plasma proteome in chickens challenged with lipopolysaccharide (LPS), a bacterial endotoxin known to stimulate the host innate immune system obtained by shotgun quantitative proteomic tandem mass tags approach using high-resolution Orbitrap technology. Statistical and bioinformatic analyses were performed to specify the effect of bacterial endotoxin. Plasma from chicken (N=6) challenged with Escherichia coli (LPS) (2 mg/kg body weight) was collected pre (0 h) and at 12, 24, 48, and 72 h post injection along with plasma from a control group (N=6) challenged with sterile saline. Protein identification and relative quantification were performed using Proteome Discoverer, and data were analysed using R. Gene Ontology terms were analysed by the Cytoscape application ClueGO based on Gallus gallus GO Biological Process database, and refined by REVIGO. Absolute quantification of several acute phase proteins, e.g. alpha-1-acid glycoprotein (AGP), serum amyloid A (SAA) and ovotrensferrin (OVT) was performed by immunoassays to validate the LC-MS results. The data contained within this article are directly related to our research article”Quantitative proteomics using tandem mass tags in relation to the acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin” [1]. The raw mass spectrometric data generated in this study were deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009399 (http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD009399)

Growth, photosynthetic pigments, phenolic content and biological activities of Foeniculum vulgare Mill., Anethum graveolens L. and Pimpinella anisum L. (Apiaceae) in response to zinc

The effect of zinc (Zn) on phenols, antioxidant activities (free radicals' scavenging activities, inhibition of lipid peroxidation, chelating activity and reducing power), and enzyme inhibition activities of acetylcholinesterase, lipoxygenase and tyrosinase of anise, dill and two cultivars of fennel [Latina" (F-L) and "Doux de Florence" (F-DF)] aqueous extracts was evaluated for the first time. At the same time, growth and photosynthetic pigment contents were also estimated. A significant decrease in all growth parameters, such as plant height, fresh and dry weights of aerial parts and roots was statistically proved in the presence of 2 mM Zn. An enhancement of Zn accumulation was observed, particularly in aerial part. Exposure to 2 mM Zn induced quantitative changes in the phenols of all tested extracts. In fennel extracts, a decrease of phenol content was observed when treated with Zn, whereas an increase was found in dill and anise extracts submitted to Zn treatment. All samples, either in the presence or absence of Zn, showed antioxidant activity, independent on the tested assay, with the exception of the cultivar F-DF (treated and non-treated), which was unable to scavenge NO radicals. Zn-treated dill and anise presented usually higher antioxidant activity. Concerning inhibitory activities against acetylcholinesterase, lipoxygenase and tyrosinase enzymes, both treated and non-treated F-DF did not inhibit acetylcholinesterase activity. Zinc-treated F-DF was also unable to inhibit lipoxygenase activity. The remaining species presented capacity for inhibiting those enzymes and tyrosinase as well, but their abilities varied in response to Zn excess.Portuguese National Funding Agency for Science, Research and Technology (Fundacao para a Ciencia e a Tecnologia - FCTPortugal) [UID/BIA/04325/2013-MEDTBIO, UID/Multi/04326/2013]info:eu-repo/semantics/publishedVersio

Region-wide glacier mass balances over the Pamir-Karakoram-Himalaya during 1999-2011 (vol 7, pg 1263, 2013)

ISI Document Delivery No.: 273OY Times Cited: 0 Cited Reference Count: 1 Cited References: Gardelle J, 2013, CRYOSPHERE, V7, P1263, DOI 10.5194/tc-7-1263-2013 Gardelle, J. Berthier, E. Arnaud, Y. Kaab, A. 0 COPERNICUS GESELLSCHAFT MBH GOTTINGEN CRYOSPHEREThe recent evolution of Pamir-Karakoram- Himalaya (PKH) glaciers, widely acknowledged as valuable high-altitude as well as mid-latitude climatic indicators, remains poorly known. To estimate the regionwide glacier mass balance for 9 study sites spread from the Pamir to the Hengduan Shan (eastern Himalaya), we compared the 2000 Shuttle Radar Topography Mission (SRTM) digital elevation model (DEM) to recent (2008- 2011) DEMs derived from SPOT5 stereo imagery. During the last decade, the region-wide glacier mass balances were contrasted with moderate mass losses in the eastern and central Himalaya (−0.22±0.12mw.e. yr−1 to −0.33±0.14mw.e. yr−1) and larger losses in the western Himalaya (−0.45±0.13mw.e. yr−1). Recently reported slight mass gain or balanced mass budget of glaciers in the central Karakoram is confirmed for a larger area (+0.10±0.16mw.e. yr−1) and also observed for glaciers in the western Pamir (+0.14±0.13mw.e. yr−1). Thus, the "Karakoram anomaly" should be renamed the "Pamir- Karakoram anomaly", at least for the last decade. The overall mass balance of PKH glaciers, −0.14±0.08mw.e. yr−1, is two to three times less negative than the global average for glaciers distinct from the Greenland and Antarctic ice sheets. Together with recent studies using ICESat and GRACE data, DEM differencing confirms a contrasted pattern of glacier mass change in the PKH during the first decade of the 21st century

Rate-dependency of action potential duration and refractoriness in isolated myocytes from the rabbit AV node and atrium

During atrial fibrillation, ventricular rate is determined by atrioventricular nodal (AVN) conduction, which in part is dependent upon the refractoriness of single AVN cells. The aims of this study were to investigate the rate-dependency of the action potential duration (APD) and effective refractory period (ERP) in single myocytes isolated from the AV node and atrium of rabbit hearts, using whole cell patch clamping, and to determine the contribution of the 4-aminopyridine (4-AP)-sensitive current, ITO1to these relationships in the two cell types. AVN cells had a more positive maximum diastolic potential (-60±1 v-71±2 mV), lower Vmax(8±2 v 144±17 V/s) and higher input resistance [420±46 v 65±7 MOHgr (mean±s.eP<0.05n=9–33)], respectively, than atrial myocytes. Stepwise increases in rate from 75 beats/min caused activation failure and Wenckebach periodicity in AVN cells (at around 400 beats/min), but 1:1 activation in atrial cells (at up to 600 beats/min). Rate reduction from 300 to 75 beats/min shortened the ERP in both cell types (from 155±7 to 135±11 ms in AVN cells [P<0.05, n=6] and from 130±8 to 106±7 ms in atrial cells [P<0.05, n=10]). Rate increase from 300 to 480 and 600 beats/min shortened ERP in atrial cells, by 12±4% (n=8) and 26±7% (n=7), respectively (P<0.05). By contrast, AVN ERP did not shorten at rates >300 beats/min. In atrial cells, rate reduction to 75 beats/min caused marked shortening of APD50(from 51±6 to 29±6 ms, P<0.05). 4-AP (1 mm) significantly prolonged atrial APD50at 75 beats/min (P<0.05, n=7), but not at 300 or 400 beats/min. In AVN cells, in contrast, there was less effect of rate change on APD, and 4-AP did not alter APD50at any rate. 4-AP also did not affect APD90or ERP in either cell type. In conclusion, a lack of ERP-shortening at high rates in rabbit single AVN cells may contribute to ventricular rate control. ITO1contributed to the APD50rate relation in atrial, but not AVN cells and did not contribute to the ERP rate relation in either cell type

Influence of the combined system FPS isolator with viscous fluid damper on the dynamic response of the isolated bridges

To illustrate the effect of the combined system FPS isolator (Fiction Pendulum System) with viscous fluid damper on the seismic response of the isolated bridges, a comparative study was realised on a bridge in three different situations. In first situation, we consider the bridge without seismic isolation, while in the second and the third situation, the bridge is successively equipped with a system of FPS isolator then with a combined system FPS isolator and viscous fluid damper. The analysis was done using the digital program of dynamic analysis of the structures SAP2000 and this study consists in comparing the responses of the bridge in terms of baseshear, displacement, acceleration and absorbed energy. The results show that the incorporation of a damping device with viscous fluid damper to the isolator allows to dissipate the maximum of energy, to control the deformations which are located at the level of the isolator and to realize a satisfying compromise between the reduction of the seismic forces and the increase of the deformations in the seismic isolation

Development and Testing of a Hot Stamped Axial Crush Member with Tailored Properties

This thesis investigates the effect of tailoring the properties of a hot stamped axial crush rail consisting of a top hat cross-section on its crash response. The tailored hot stamping (THS) process is considered, in which the forming tool is partitioned into zones that are either cooled or heated. The local die temperature controls the quench rate within the part, such that the formed part will have zones of differing hardness (strength) and ductility levels. In the current work, four configurations of THS rails were formed: a non-tailored configuration using conventional room temperature tooling and three tailored configurations in which one-half of the rail was quenched while the other half was formed in tooling that was heated at different temperatures (in the range 400-700˚C). Micro-hardness measurements confirmed that THS can be used to form an axial crush member that contains tailored properties along its length. The asformed parts exhibited a Vickers hardness of ~475 HV in zones formed in cooled tooling, ~280 HV in zones formed in tooling that was heated to 400˚C and ~215 HV in zones that were formed when the die set was heated to 700˚C. The formed parts were then welded in pairs along their flanges and axially crushed under dynamic and quasi-static conditions. The non-tailored, fully hardened components did absorb the highest energy (25.8 kJ), but exhibited extensive tearing and fracture. The force-displacement and energy absorption curves from the crush experiments showed that the tailored configurations absorbed less energy (21.6-25.7 kJ), but were less susceptible to buckling and had excellent repeatability in its crash response. Tailored regions with higher temperature tooling and/or graded tooling temperature distributions exhibited the least damage and absorbed the highest energy amongst the tailored configurations. Numerical models of the THS process and subsequent crash response were developed using the Åkerström material model in LS-Dyna to model the forming and the constitutive models of Bardelcik et al. to simulate the crash behavior. The predicted micro-hardness distributions in the components with bainite or martensite as-formed phases were very accurate (usually within 10%), whereas predictions for ferrite-containing components were within 15% of the measured hardness. The crash models were able to accurately predict the energy absorption of rails that were formed in cooled and 400˚C tooling. They were less accurate for dynamic crush of rails that contained ferrite. Overall, tailoring was found to have a positive effect on the energy absorption of a hot stamped axial crush member. Future work should focus on improving the hot stamping and crash models so they can accurately account for the effect of ferrite. Furthermore, a non-axial, angled offset crush should also be performed to more thoroughly investigate the effects of tailoring

Acute phase proteins and stress markers in the immediate response to a combined vaccination against Newcastle disease and infectious bronchitis viruses in specific pathogen free (SPF) layer chicks

Vaccination is an important tool in poultry health, but is itself a stressor often resulting in a reduction in feed intake, body weight gain, and nutrient digestibility. In other species, vaccination is associated with an immediate acute-phase response. As an important immune parameter, the circulating heterophil/lymphocyte (H/L) ratio is a well-recognized parameter of stress in poultry. In this study, the effects of a routinely used commercial poultry vaccine on the acute phase response (APR) and H/L ratios in specific pathogen-free (SPF) layer chicks was examined to determine if post vaccination (PV) stress and an APR occur. A combined Newcastle disease and infectious bronchitis vaccine (Nobalis Ma5+Clone 30) was administered to SPF chicks by the intraocular route at age 7 d. Acute phase proteins (APP), alpha-1 acid glycoprotein (AGP) and serum amyloid A (SAA) were measured by enzyme-linked immunosorbent assays at d 0 (pre-vaccination) and d 0.5, 1, 2, 3, 4, 5, 6, and 21 PV. Stress was determined in the chicks by measurement of the H/L ratio. The immune response to the vaccine was estimated by measurement of the antibody (IgY) response to the vaccine at d 21. The antibody titer was significantly (P < 0.05) higher in the vaccinated group at 21 d PV, confirming stimulation of the immune system. The H/L ratio was also significantly higher in the vaccinated group at 1 to 2 d (P < 0.01) and at 3 d (P < 0.05) PV. The concentration of SAA increased by 2.8-fold, from 63.7 μg/mL in controls to 181 μg/mL in the vaccinated group, (P < 0.05) at 1 d PV. AGP increased 1.6-fold at 2 d PV, (from 0.75 g/mL in the control group to 1.24 g/mL in the vaccinated group, P < 0.05). In conclusion an immediate but mild APR occurred in the chicks following intraocular vaccination, whereas the stress response as measured by H/L ratio seemed to be more specific and sensitive. Measurement of these biomarkers of the host response could be a tool in vaccine development

The Utility of Non-Specific ECG Findings in the Setting of Low High-Sensitivity Cardiac Troponin Levels

Objective: Our objective was to assess the relationship between non-specific ischemic electrocardiogram (nsi-ECG) findings and the occurrence of major adverse cardiac events (MACE) within a 30-day timeframe among patients in the Emergency Department (ED) with low high-sensitivity cardiac troponin (hs-cTnI) levels. Methods: We conducted a secondary analysis of the RACE-IT trial, a randomized trial performed across 9 EDs from July 2020 through March 2021 that looked at the effectiveness of hs-cTnI in evaluating the risk for acute myocardial infarction (AMI). Our study assessed the association between nsi-ECG findings (left bundle branch block, ST-segment changes, or T-wave inversions) and 30-day MACE (death, AMI, heart failure hospitalization, or coronary revascularization) in patients who had AMI ruled out based on low hs-cTnI levels. Results: 16,606 patients were included in this analysis. Combined, there were 3345 patients with potentially ischemic ECG findings. Thirty-day death or AMI occurred in 66 patients. Death within 30 days occurred in 47 patients, of whom 38 were adjudicated as non-cardiac. There was no difference in MACE events based on potentially ischemic findings (OR 1.38, 95% CI 0.79 - 2.39, p=0.257). The presence of ST-segment changes, however, had a trend towards greater odds of MACE (OR 2.53, 95% CI 0.92 - 6.99). Conclusion: Non-specific ischemic ECG findings in the setting of low hs-cTnI are not associated with greater MACE events within 30 days of discharge for patients with possible AMIs. The use of nsi-ECG findings should be considered in the context of hs-cTnI levels when evaluating risk for coronary disease