Quantitative estimates of glacial refugia for chimpanzees ( Pan troglodytes ) since the Last Interglacial (120,000 BP)

Paleoclimate reconstructions have enhanced our understanding of how past climates have shaped present-day biodiversity. We hypothesize that the geographic extent of Pleistocene forest refugia and suitable habitat fluctuated significantly in time during the late Quaternary for chimpanzees (Pan troglodytes). Using bioclimatic variables representing monthly temperature and precipitation estimates, past human population density data, and an extensive database of georeferenced presence points, we built a model of changing habitat suitability for chimpanzees at fine spatio-temporal scales dating back to the Last Interglacial (120,000 BP). Our models cover a spatial resolution of 0.0467° (approximately 5.19 km2 grid cells) and a temporal resolution of between 1000 and 4000 years. Using our model, we mapped habitat stability over time using three approaches, comparing our modeled stability estimates to existing knowledge of Afrotropical refugia, as well as contemporary patterns of major keystone tropical food resources used by chimpanzees, figs (Moraceae), and palms (Arecacae). Results show habitat stability congruent with known glacial refugia across Africa, suggesting their extents may have been underestimated for chimpanzees, with potentially up to approximately 60,000 km2 of previously unrecognized glacial refugia. The refugia we highlight coincide with higher species richness for figs and palms. Our results provide spatio-temporally explicit insights into the role of refugia across the chimpanzee range, forming the empirical foundation for developing and testing hypotheses about behavioral, ecological, and genetic diversity with additional data. This methodology can be applied to other species and geographic areas when sufficient data are available

Hyperglycaemia in critical illness is a risk factor for later development of type II diabetes mellitus

Hyperglycaemia caused by stress and inflammation is common during critical illness. We hypothesised that a latent glucose metabolism disturbance contributes to development of hyperglycaemia and that those patients have increased risk for diabetes. We included patients with sepsis, acute coronary syndrome and acute heart failure with no history of impaired glucose metabolism and divided them in the hyperglycaemia group (glucose ≥ 7.8 mmol/l) and normoglycaemia group. Patients were followed for 5 years. Follow-up was completed for 115 patients in the normoglycaemia group, of which 4 (3.5%) developed type 2 diabetes. In the hyperglycaemia group 51 patients finished follow-up and 8 (15.7%) developed type 2 diabetes. Relative risk in 5-year period for patients with hyperglycaemia was 4.51 for development of type 2 diabetes. Patients with hyperglycaemia during critical illness who are not diagnosed with diabetes before or during the hospitalisation should be considered a population at increased risk for developing diabetes

Clients' Perception of Outcome of Team-Based Prenatal and Reproductive Genetic Counseling in Serbian Service Using the Perceived Personal Control (PPC) Questionnaire

This is the first study in Serbia and the region of South-East Europe dedicated to clients' perception of outcome and efficiency of prenatal and reproductive genetic counseling. The primary aim of this study was to assess overall value and success of genetic counseling in prenatal and reproductive care with regard to perceived personal control of clients, reflecting also in a part patient comprehension, knowledge retention, and empowerment in decision-making. The standardized Perceived Personal Control questionnaire (PPC) was used for the assessment of 239 female participants. First, we performed a complete validation of the psychometric characteristics of the Serbian-language version of the PPC questionnaire. The validation of the questionnaire permits other researchers from Serbian-speaking regions of South-East Europe to use this standard instrument to assess the effectiveness of prenatal genetic counseling in their communities and analyze advantages and disadvantages of their counseling models. We also measured social and demographic characteristics of participants. Further, we analyzed effects of our team-based prenatal and reproductive genetic counseling model through (a) calculation of PPC scores at three different stages (before initial, after initial, and before second counseling session), and (b) by assessing participants' responses by indication for referral (advanced maternal age, abnormal biochemical screening, family history of hereditary disorders, maternal exposure to drugs, exposure to radiation, exposure to infective agents, infertility or recurrent abortions, and miscellaneous). The results indicate that participants' knowledge after initial counseling increased significantly and after that remained stable and sustainable. A satisfactory level of confidence among participants had been achieved, in that many felt an increased sense of control over their situation and emotional response to it. Indirectly, these results indicate the success of a team-based prenatal genetic counseling model, which has not been assessed in the literature to date

Spontaneous activity responses to exercise in males and females

This study examines the impact of a short-term exercise programme, prescribed on the basis of current exercise recommendations, on energy balance in males and females to assess whether this type of exercise induces compensatory changes in spontaneous activity energy expenditure (SAEE) and energy intake (EI). Individuals were monitored for 16 days, 8 days of habitual physical activity (C) and 8 days when exercise was imposed (E). Total energy expenditure (TEE) was calculated from individual relationships of O2 and CO2 to heart rate (HR) records of HR and physical activity obtained during waking hours of the C and E periods and basal metabolic rate (BMR) measurements (Deltatrac System, Datex Instrumentation). Changes in nude body mass (BM) were estimated by using a digital scale (Sartorius AG, Gottigen, Germany). Laboratory and free-living. Eight lean females (body fat: 17.54.5%) and eight males of similar percentage body fat participated in this study. All subjects were Caucasian and aged between 20 and 25 years. During the E period, a supervised exercise session was conducted every second day, each consisting of a total net energy expenditure of 2092 kJ+BMR at 90% lactate threshold. During the E period, TEE was higher than C in males and females (exercise: 95.213.9, 78.315.9 MJ; control: 82.410.4, 68.816.7 MJ, respectively; P<0.00; P=0.02). SAEE, calculated as TEE minus the energy expended during exercise, was not significantly different between C (males: 82.44.8 MJ; females: 68.87.6 MJ) and E (males: 86.86.3 MJ; females: 70.07.2 MJ) periods in either gender. Males showed no change in BM over the C (pre-intervention: 83.47.2 kg; post-intervention: 83.16.8 kg) or E (pre-intervention: 83.46.8 kg; post-intervention: 83.46.8 kg) periods. Females' BM over the C period did not alter (pre-intervention: 63.32.8 kg; post-intervention 63.73.1 kg); however, there was a significant decrease (P<0.00) in BM over the E period (pre-intervention: 63.02.7 kg; post-intervention: 62.42.7 kg). The exercise programme was achieved in males and females without any impact on SAEE. Therefore, differences between genders in relation to BM reduction can be explained by differences in the EI response to exercise

Composição corporal em crianças de sete a 10 anos de idade, de alto nível socioeconômico Body composition in seven-to-ten-year old children of high socioeconomic status

O objetivo deste estudo foi analisar a composição corporal de crianças de sete a 10 anos de idade, de alto nível socioeconômico. Para tanto, 511 crianças (274 meninos e 237 meninas) foram submetidas a medidas antropométricas de massa corporal, estatura e espessuras de dobras cutâneas tricipital (TR) e subescapular (SE). Com base nessas informações, foram determinados: gordura corporal relativa (% Gordura), massa corporal magra (MCM), distribuição da gordura corporal (DGC) e o somatório da espessura das dobras cutâneas TR e SE (&#931;EDC). Anova two-way foi utilizada para as comparações entre os sexos e os diferentes grupos etários, seguida pelo teste post hoc de Scheffé quando P < 0,05. Para a classificação das crianças, de acordo com as categorias de adiposidade corporal, foi utilizada distribuição de freqüência. O teste de comparação entre proporções foi adotado para verificar as diferenças entre os sexos, em cada categoria (P < 0,05). Diferenças significantes entre os sexos (P < 0,05) foram verificadas na dobra TR (meninas > meninos) e na MCM (meninos > meninas). Efeito significante da idade foi identificado em todas as variáveis analisadas, exceto na DGC, com os valores mais elevados sendo encontrados no grupo etário de 10 anos. Um percentual elevado, tanto de meninos quanto de meninas, apresentou índice de adiposidade alto, com superioridade para os meninos (28% vs. 14%, P < 0,01). Por outro lado, um contingente maior de meninas apresentou concentrações de gordura corporal relativa abaixo dos pontos de corte desejáveis (15% vs. 3%, P < 0,01). Os valores de adiposidade corporal encontrados no presente estudo sugerem que um percentual elevado de crianças, de ambos os sexos, já apresenta importantes fatores de risco à saúde, em idades precoces.<br>The objective of this study was to analyze the body composition of seven to 10 year-old children of high socioeconomic status. Therefore, 511 children (274 boys and 237 girls) were submitted to body mass, height and triceps (TR) and subscapular (SE) skinfolds thickness anthropometric measurement. Based on this information, the following topics were determined: relative body fat (% Fat), lean body mass (LBM), body fat distribution (BFD) and sum of the thickness of the TR and SE skinfolds (£TSF). Two-way ANOVA was used for comparisons between gender and the different age groups, followed by the Scheffé post hoc test when P < 0.05. For the children classification according to the categories of body adiposity, frequency distribution was used. A test of comparison between ratios was adopted to verify the differences between gender in each category (P < 0.05). Significant differences between gender (P < 0.05) were verified in TR skinfold (girls > boys) and in LBM (boys > girls). Significant age effect was identified in all analyzed variables, except for DBF, with the highest values having been found in the 10 year-old group. A high percentage both for boys and girls presented high adiposity index, with superiority for boys (28% vs. 14%, P < 0.01). On the other hand, a larger number of girls presented relative body fat concentrations below the desirable cutting points (15% vs. 3%, P < 0.01). The values of body adiposity found in the present study suggest that a high percentage of children from both gender already present important health risk factors at early ages