The Neuro-Glial Properties of Adipose-Derived Adult Stromal (ADAS) Cells Are Not Regulated by Notch 1 and Are Not Derived from Neural Crest Lineage

We investigated whether adipose-derived adult stromal (ADAS) are of neural crest origin and the extent to which Notch 1 regulates their growth and differentiation. Mouse ADAS cells cultured in media formulated for neural stem cells (NSC) displayed limited capacity for self-renewal, clonogenicity, and neurosphere formation compared to NSC from the subventricular zone in the hippocampus. Although ADAS cells expressed Nestin, GFAP, NSE and Tuj1 in vitro, exposure to NSC differentiation supplements did not induce mature neuronal marker expression. In contrast, in mesenchymal stem cell (MSC) media, ADAS cells retained their ability to proliferate and differentiate beyond 20 passages and expressed high levels of Nestin. In neuritizing cocktails, ADAS cells extended processes, downregulated Nestin expression, and displayed depolarization-induced Ca2+ transients but no spontaneous or evoked neural network activity on Multi-Electrode Arrays. Deletion of Notch 1 in ADAS cell cultures grown in NSC proliferation medium did not significantly alter their proliferative potential in vitro or the differentiation-induced downregulation of Nestin. Co-culture of ADAS cells with fibroblasts that stably expressed the Notch ligand Jagged 1 or overexpression of the Notch intracellular domain (NICD) did not alter ADAS cell growth, morphology, or cellular marker expression. ADAS cells did not display robust expression of neural crest transcription factors or genes (Sox, CRABP2, and TH); and lineage tracing analyses using Wnt1–Cre;Rosa26R-lacZ or -EYFP reporter mice confirmed that fewer than 2% of the ADAS cell population derived from a Wnt1-positive population during development. In summary, although media formulations optimized for MSCs or NSCs enable expansion of mouse ADAS cells in vitro, we find no evidence that these cells are of neural crest origin, that they can undergo robust terminal differentiation into functionally mature neurons, and that Notch 1 is likely to be a key regulator of their cellular and molecular characteristics

Aerosol radiative properties in the semiarid Western United States

Characterization of aerosol optical properties, such as aerosol optical depth, Angstrom exponent, and volume size distribution at the semiarid site of Tombstone Arizona (31°23′N, 110°05′W, 1408 m) will be presented for one annual cycle. In this region, extensive observations of selected optical parameters such as aerosol optical depth (AOD) have been made in the past and reported on in the literature. Less is known about other optical characteristics that are important in climate modeling and remote sensing. New observational techniques and inversion methods allow for the expansion of the earlier information. Observations have been taken with a state of the art sun photometer for a 1-year period and their analysis will be presented here. Monthly mean AODs at 500 nm were found to be in the range of 0.03–0.12; the monthly mean Angstrom exponent ranged from 0.9 to 1.6, being higher in spring and summer and lower in late fall and winter. Volume size distributions exhibit clear dominance of smaller particles, with a gradual increase in size from winter to spring and into summer. Annual variation of the radii of the smaller and the larger particles ranged between 0.05–0.4 and 4–8 μm, respectively. Radiance measurements at 940 nm were used to estimate precipitable water. The retrieved values compared within limits of uncertainty with independently derived estimates from the National Center for Environmental Prediction (NCEP) regional weather forecast model. An interesting outcome from this study was the consistency found in aerosol optical depths as observed in this study and those derived about two decades ag