163 research outputs found
Properties of optically selected BL Lac candidates from the SDSS
\textbf{Context.} Deep optical surveys open the avenue for find large numbers
of BL Lac objects that are hard to identify because they lack the unique
properties classifying them as such. While radio or X-ray surveys typically
reveal dozens of sources, recent compilations based on optical criteria alone
have increased the number of BL Lac candidates considerably. However, these
compilations are subject to biases and may contain a substantial number of
contaminating sources. \textbf{Aims.} In this paper we extend our analysis of
182 optically selected BL Lac object candidates from the SDSS with respect to
an earlier study. The main goal is to determine the number of bona fide BL Lac
objects in this sample. \textbf{Methods.} We examine their variability
characteristics, determine their broad-band radio-UV SEDs, and search for the
presence of a host galaxy. In addition we present new optical spectra for 27
targets with improved S/N with respect to the SDSS spectra. \textbf{Results.}
At least 59% of our targets have shown variability between SDSS DR2 and our
observations by more than 0.1-0.27 mag de- pending on the telescope used. A
host galaxy was detected in 36% of our targets. The host galaxy type and
luminosities are consistent with earlier studies of BL Lac host galaxies.
Simple fits to broad-band SEDS for 104 targets of our sample derived
synchrotron peak frequencies between with a peak at
. Our new optical spectra do not reveal any new
redshift for any of our objects. Thus the sample contains a large number of
bona fide BL Lac objects and seems to contain a substantial fraction of
intermediate-frequency peaked BL Lacs.Comment: Accepted for publication in A\&
Bose-Einstein condensation in a circular waveguide
We have produced Bose-Einstein condensates in a ring-shaped magnetic
waveguide. The few-millimeter diameter non-zero bias ring is formed from a
time-averaged quadrupole ring. Condensates which propagate around the ring make
several revolutions within the time it takes for them to expand to fill the
ring. The ring shape is ideally suited for studies of vorticity in a
multiply-connected geometry and is promising as a rotation sensor.Comment: 4 pages, 4 figure
Bound States and Threshold Resonances in Quantum Wires with Circular Bends
We study the solutions to the wave equation in a two-dimensional tube of unit
width comprised of two straight regions connected by a region of constant
curvature. We introduce a numerical method which permits high accuracy at high
curvature. We determine the bound state energies as well as the transmission
and reflection matrices, and and focus on the nature of
the resonances which occur in the vicinity of channel thresholds. We explore
the dependence of these solutions on the curvature of the tube and angle of the
bend and discuss several limiting cases where our numerical results confirm
analytic predictions.Comment: 24 pages, revtex file, one style file and 17 PostScript figures
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Iron is not everything: unexpected complex metabolic responses between iron-cycling microorganisms
Magnetic trapping of ultracold neutrons
Three-dimensional magnetic confinement of neutrons is reported. Neutrons are
loaded into an Ioffe-type superconducting magnetic trap through inelastic
scattering of cold neutrons with 4He. Scattered neutrons with sufficiently low
energy and in the appropriate spin state are confined by the magnetic field
until they decay. The electron resulting from neutron decay produces
scintillations in the liquid helium bath that results in a pulse of extreme
ultraviolet light. This light is frequency downconverted to the visible and
detected. Results are presented in which 500 +/- 155 neutrons are magnetically
trapped in each loading cycle, consistent with theoretical predictions. The
lifetime of the observed signal, 660 s +290/-170 s, is consistent with the
neutron beta-decay lifetime.Comment: 17 pages, 18 figures, accepted for publication in Physical Review
CD20 and CD19 targeted vectors induce minimal activation of resting B lymphocytes
B lymphocytes are an important cell population of the immune system. However, until recently it was not possible to transduce resting B lymphocytes with retro- or lentiviral vectors, making them unsusceptible for genetic manipulations by these vectors. Lately, we demonstrated that lentiviral vectors pseudotyped with modified measles virus (MV) glycoproteins hemagglutinin, responsible for receptor recognition, and fusion protein were able to overcome this transduction block. They use either the natural MV receptors, CD46 and signaling lymphocyte activation molecule (SLAM), for cell entry (MV-LV) or the vector particles were further modified to selectively enter via the CD20 molecule, which is exclusively expressed on B lymphocytes (CD20-LV). It has been shown previously that transduction by MV-LV does not induce B lymphocyte activation. However, if this is also true for CD20-LV is still unknown. Here, we generated a vector specific for another B lymphocyte marker, CD19, and compared its ability to transduce resting B lymphocytes with CD20-LV. The vector (CD19ds-LV) was able to stably transduce unstimulated B lymphocytes, albeit with a reduced efficiency of about 10% compared to CD20-LV, which transduced about 30% of the cells. Since CD20 as well as CD19 are closely linked to the B lymphocyte activation pathway, we investigated if engagement of CD20 or CD19 molecules by the vector particles induces activating stimuli in resting B lymphocytes. Although, activation of B lymphocytes often involves calcium influx, we did not detect elevated calcium levels. However, the activation marker CD71 was substantially up-regulated upon CD20-LV transduction and most importantly, B lymphocytes transduced with CD20-LV or CD19ds-LV entered the G1b phase of cell cycle, whereas untransduced or MV-LV transduced B lymphocytes remained in G0. Hence, CD20 and CD19 targeting vectors induce activating stimuli in resting B lymphocytes, which most likely renders them susceptible for lentiviral vector transduction
CerebNet: A fast and reliable deep-learning pipeline for detailed cerebellum sub-segmentation
Quantifying the volume of the cerebellum and its lobes is of profound interest in various neurodegenerative and acquired diseases. Especially for the most common spinocerebellar ataxias (SCA), for which the first antisense oligonculeotide-base gene silencing trial has recently started, there is an urgent need for quantitative, sensitive imaging markers at pre-symptomatic stages for stratification and treatment assessment. This work introduces CerebNet, a fully automated, extensively validated, deep learning method for the lobular segmentation of the cerebellum, including the separation of gray and white matter. For training, validation, and testing, T1-weighted images from 30 participants were manually annotated into cerebellar lobules and vermal sub-segments, as well as cerebellar white matter. CerebNet combines FastSurferCNN, a UNet-based 2.5D segmentation network, with extensive data augmentation, e.g. realistic non-linear deformations to increase the anatomical variety, eliminating additional preprocessing steps, such as spatial normalization or bias field correction. CerebNet demonstrates a high accuracy (on average 0.87 Dice and 1.742mm Robust Hausdorff Distance across all structures) outperforming state-of-the-art approaches. Furthermore, it shows high test-retest reliability (average ICC >0.97 on OASIS and Kirby) as well as high sensitivity to disease effects, including the pre-ataxic stage of spinocerebellar ataxia type 3 (SCA3). CerebNet is compatible with FreeSurfer and FastSurfer and can analyze a 3D volume within seconds on a consumer GPU in an end-to-end fashion, thus providing an efficient and validated solution for assessing cerebellum sub-structure volumes. We make CerebNet available as source-code (https://github.com/Deep-MI/FastSurfer)
Options for the Neutron Lifetime Measurements in Traps
Different geometries for the neutron lifetime measurements by the method of
ultracold neutron storage in material traps and additional possibilities for
the neutron storage in the magnetic storage ring are considered.Comment: 21 p., 11 figure
Squalamine: An Appropriate Strategy against the Emergence of Multidrug Resistant Gram-Negative Bacteria?
We reported that squalamine is a membrane-active molecule that targets the membrane integrity as demonstrated by the ATP release and dye entry. In this context, its activity may depend on the membrane lipid composition. This molecule shows a preserved activity against bacterial pathogens presenting a noticeable multi-resistance phenotype against antibiotics such as polymyxin B. In this context and because of its structure, action and its relative insensitivity to efflux resistance mechanisms, we have demonstrated that squalamine appears as an alternate way to combat MDR pathogens and by pass the gap regarding the failure of new active antibacterial molecules
To transduce a zebra finch: interrogating behavioral mechanisms in a model system for speech
The ability to alter neuronal gene expression, either to affect levels of endogenous molecules or to express exogenous ones, is a powerful tool for linking brain and behavior. Scientists continue to finesse genetic manipulation in mice. Yet mice do not exhibit every behavior of interest. For example, Mus musculus do not readily imitate sounds, a trait known as vocal learning and a feature of speech. In contrast, thousands of bird species exhibit this ability. The circuits and underlying molecular mechanisms appear similar between disparate avian orders and are shared with humans. An advantage of studying vocal learning birds is that the neurons dedicated to this trait are nested within the surrounding brain regions, providing anatomical targets for relating brain and behavior. In songbirds, these nuclei are known as the song control system. Molecular function can be interrogated in non-traditional model organisms by exploiting the ability of viruses to insert genetic material into neurons to drive expression of experimenter-defined genes. To date, the use of viruses in the song control system is limited. Here, we review prior successes and test additional viruses for their capacity to transduce basal ganglia song control neurons. These findings provide a roadmap for troubleshooting the use of viruses in animal champions of fascinating behaviors-nowhere better featured than at the 12th International Congress
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