Life history and morphological studies of Punctaria tenuissima (Chordariaceae, Phaeophyceae), a new record for the Azores

Copyright © 2010 by Walter de Gruyter.Punctaria tenuissima (Chordariaceae, Phaeophyceae) is reported for the first time from the Azores. Erect thalli were collected on the Island of Sa˜o Miguel; they were up to 3 cm long, flattened and often twisted. The plurilocular sporangia were formed from surface cells that were quadrate or rectangular in surface view. Unilocular sporangia were not observed in the field. In culture, the plurispores of P. tenuissima developed into Hecatonema-like, tufted, prostrate thalli that formed plurilocular sporangia. The plurispores of the prostrate thalli cultured at 158C with a long day (LD) photoperiod developed into new prostrate thalli, which in turn formed plurilocular sporangia. This cycle was repeated 5 times, resulting in several generations of reproductive prostrate thalli. When these cultures were cooled to 108C with a short day (SD) photoperiod, new erect thalli developed from the prostrate thalli resembling the thalli collected in the field. These erect blades produced unilocular and plurilocular sporangia on the same or on different thalli. The plurispores and unispores produced by the erect thalli at 108C under SD conditions once again developed into new prostrate thalli. This is the first report of unilocular sporangia formed in cultures of P. tenuissima. Sexual reproduction was not observed. The culture conditions, particularly temperature and daylength, appeared to influence the formation of erect blades. The Punctaria-like thalli were produced in 108C/SD conditions, whilst the Hecatonema-like thalli were produced under 108–158C/LD and SD conditions. These results are similar to those reported for P. tenuissima from other locations and suggest the occurrence of both direct and heteromorphic life histories in the Azorean algae

Advanced backcross QTL mapping of resistance to Fusarium head blight and plant morphological traits in a Triticum macha × T. aestivum population

While many reports on genetic analysis of Fusarium head blight (FHB) resistance in bread wheat have been published during the past decade, only limited information is available on FHB resistance derived from wheat relatives. In this contribution, we report on the genetic analysis of FHB resistance derived from Triticum macha (Georgian spelt wheat). As the origin of T. macha is in the Caucasian region, it is supposed that its FHB resistance differs from other well-investigated resistance sources. To introduce valuable alleles from the landrace T. macha into a modern genetic background, we adopted an advanced backcross QTL mapping scheme. A backcross-derived recombinant-inbred line population of 321 BC2F3 lines was developed from a cross of T. macha with the Austrian winter wheat cultivar Furore. The population was evaluated for Fusarium resistance in seven field experiments during four seasons using artificial inoculations. A total of 300 lines of the population were genetically fingerprinted using SSR and AFLP markers. The resulting linkage map covered 33 linkage groups with 560 markers. Five novel FHB-resistance QTL, all descending from T. macha, were found on four chromosomes (2A, 2B, 5A, 5B). Several QTL for morphological and developmental traits were mapped in the same population, which partly overlapped with FHB-resistance QTL. Only the 2BL FHB-resistance QTL co-located with a plant height QTL. The largest-effect FHB-resistance QTL in this population mapped at the spelt-type locus on chromosome 5A and was associated with the wild-type allele q, but it is unclear whether q has a pleiotropic effect on FHB resistance or is closely linked to a nearby resistance QTL

Data from: Comparison of a newly established emotional stimulus approach to a classical assessment-driven approach in BLS training: a randomised controlled trial

Objective: The study objective was to implement two strategies (short emotional stimulus vs announced practical assessment) in the teaching of resuscitation skills in order to evaluate whether one led to superior outcomes. Setting: This study is an educational intervention provided in one German academic university hospital. Participants: First-yearmedical students (n=271) during the first3 weeks of their studies. Interventions: Participants were randomly assigned to one of two groups following a sequence of random numbers: the emotional stimulus group (EG) and the assessment group (AG). In the EG, the intervention included watching an emotionally stimulating video prior to the Basic Life Support (BLS) course. In the AG, a practical assessment of the BLS algorithm was announced and tested within a 2 min simulated cardiac arrest scenario. After the baseline testing, a standardised BLS course was provided. Evaluation points were defined 1 week and 6 months after. Primary outcome measures: Compression depth (CD) and compression rate (CR) were recorded as the primary endpoints for BLS quality. Results: Within the study, 137 participants were allocated to the EG and 134 to the AG. 104 participants from EG and 120 from AG were analysed1 week after the intervention, where they reached comparable chest-compression performance without significant differences (CR P=0.49; CD P=0.28). The chest-compression performance improved significantly for the EG (P<0.01) and the AG (P<0.01) while adhering to the current resuscitation guidelines criteria for CD and CR. Conclusions: There was no statistical difference between both groups’ practical chest-compression-performance. Nevertheless, the 2 min video sequence used in the EG with its low production effort and costs, compared with the expensive assessment approach, provides broad opportunities for applicability in BLS training