Hemolymph proteins and reproduction in Periplaneta americana: the nature of conjugated proteins and the effect of cardiac-allatectomy on protein metabolism

1. The nature and behavior of the conjugated proteins in the hemolymph of adult female Periplaneta americana (L.) have been studied in relation to the ovarian cycle, using disc electrophoresis in polyacrylamide gel and various staining procedures. It is shown that there may be not only a change in the relative concentration of the different protein fractions with oocyte growth, but also differences in their chemical composition. Fraction 3 and the female fraction 4, which represent the major proteins in the blood, appear to serve as carriers of lipids to the ovaries, besides most likely providing proteinaceous yolk precursors to the same. Fractionations made with ovarian homogenates seem to indicate that the lipids and sugars bound to such proteins may become freed at entry into the ovary. 2. Cardiac-allatectomy has been found to result in a pronounced accumulation of proteins in the blood, and also in changes in their chemical composition. In such animals there is a tendency with aging for the loss of lipid prosthetic groups and for several of the fractions including the major proteins to stain preferentially for carbohydrates. This effect could be reversed by the implantation of fresh cardiacum-allatum complexes taken from adult females. It is suggested that in cardiac-allatectomized females there is probably a bilateral arrest of lipid and protein metabolism, and that the vitellogenic proteins already synthesized. but not utilized by the ovary, become converted into glycoproteins. 3. Fractionation studies on fat body homogenates of females in different stages of ovarian activity appear to indicate that in Periplaneta americana none of the soluble lipo- or glycoproteins, including the female fraction, is as such synthesized or stored in the fat body

Existing and proposed marine parks and reserves in India - a review

In many of the developed and lesser developed countries the awareness for the creation of Marine Parks and reserves has never been greater than it is today. In India which has a tradition of established Wildlife Sanctuaries and Conservation Projects, the concept of Marine Parks and reserves is new. Nevertheless, we are seized of the urgency of the problem

Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus

New neurons are generated in the postnatal rodent hypothalamus, with a subset of tanycytes in the third ventricular (3V) wall serving as neural stem/progenitor cells. However, the precise stem cell niche organization, the intermediate steps and the endogenous regulators of postnatal hypothalamic neurogenesis remain elusive. Quantitative lineage-tracing in vivo revealed that conditional deletion of fibroblast growth factor 10 (Fgf10) from Fgf10-expressing beta-tanycytes at postnatal days (P)4-5 results in the generation of significantly more parenchymal cells by P28, composed mostly of ventromedial and dorsomedial neurons and some glial cells, which persist into adulthood. A closer scrutiny in vivo and ex vivo revealed that the 3V wall is not static and is amenable to cell movements. Furthermore, normally beta-tanycytes give rise to parenchymal cells via an intermediate population of alpha-tanycytes with transient amplifying cell characteristics. Loss of Fgf10 temporarily attenuates the amplification of beta-tanycytes but also appears to delay the exit of their alpha-tanycyte descendants from the germinal 3V wall. Our findings suggest that transience of cells through the alpha-tanycyte domain is a key feature, and Fgf10 is a negative regulator of postnatal hypothalamic neurogenesis.Peer reviewe

Surface Tension of Seawater

New measurements and a reference correlation for the surface tension of seawater at atmospheric pressure are presented in this paper. Surface tension of seawater was measured across a salinity range of 20 ⩽ S ⩽ 131 g/kg and a temperature range of 1 ⩽ t ⩽ 92 °C at atmospheric pressure using the Wilhelmy plate method. The uncertainty within measurements varied from 0.18 to 0.37 mN/m with the average uncertainty being 0.22 mN/m. The experimental procedures were validated with tests conducted on ACS reagent grade water and aqueous sodium chloride solutions. Literature data and present measurements were evaluated and a reference correlation was developed expressing surface tension of seawater as a function of temperature and salinity. The average absolute percentage deviation between measurements and the correlation was 0.19% while the maximum deviation was 0.60%.Center for Clean Water and Clean Energy at MIT and KFUPM (Project R13-CW-10

Multicenter experience from the UK and Ireland of use of lumen-apposing metal stent for transluminal drainage of pancreatic fluid collections

Background and study aims: Pancreatic fluid collection (PFC) is a common complication of pancreatitis for which endoscopic ultrasound-guided drainage is first-line treatment. A new single-device, lumen-apposing, covered self-expanding metal stent (LAMS) has been licensed for PFC drainage. We therefore present our multicenter experience with the LAMS for PFC drainage in a multicenter prospective case series to assess success and complication rates. Patients and methods: All adult patients from 11 tertiary centers who had LAMS placement for PFC from July 2015 to July 2016 were included. Data including indications, technical success, clinical success, collection resolution, stent removal, early and late adverse events (AEs), mortality and recurrence at 6 months were collected. Results: 116 patients, median age 52.5 years (range 16 – 80) and 67 % male, were treated with a single LAMS in each case. The indication was walled off necrosis (WON) in 70 and pseudocyst in 46. Median size of the PFC was 11 cm (5 – 21 cm) and the estimated median necrotic volume in WON was 30 % (5 % – 90 %). Stent insertion was technically successful in 115 (99.1 %) and clinically successful in 109 (94 %). Early serious AEs (SAEs): n = 7 sepsis, n = 1 stent blockage with food, n = 1 stent migration requiring laparotomy, n = 1 stent dislodgement and n = 1 bleeding requiring emboliZation. Late AEs: n = 1 buried stent and n = 1 esophageal fistula. Non-procedure-related deaths: n = 3 (2.5 %). Conclusion: This multicenter case series demonstrates that use of the new LAMS is feasible, effective and relatively safe in draining PFC with a technical success rate of 99 % and cumulative SAE rate of 11.2 %

A multicentre evaluation and expert recommendations of use of the newly developed BioFire Joint Infection polymerase chain reaction panel.

Septic arthritis is a serious condition with significant morbidity and mortality, routinely diagnosed using culture. The FDA has recently approved the rapid molecular BioFire® Joint Infection Panel (BJIP) for synovial fluid. We aimed to evaluate the BJIP compared to culture and its potential use in patient management. A multicentre retrospective evaluation of BJIP was conducted in the UK and Ireland. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated between the BJIP and routine culture. A multidisciplinary team (MDT) discussion addressing the optimal or potential case use of the assay practice was facilitated. Three hundred ninety-nine surplus synovial fluid samples (~ 70% from native joints) from eight centres were processed using BJIP in addition to routine culture. An increased yield of positive results was detected using BJIP compared to routine culture (98 vs 83), giving an overall PPA of 91.6% and overall NPA of 93% for the BJIP compared to culture results. The BJIP detected resistant markers and additional organisms that could influence antibiotic choices including Neisseria gonorrhoeae and Kingella kingae. The MDT agreed that the assay could be used, in addition to standard methods, in adult and children patients with specialist advice use based on local needs. Rapid results from BJIP were assessed as having potential clinical impact on patient management. Organisms not included in the panel may be clinically significant and may limit the value of this test for PJI

SARS-CoV-2 infection in acute pancreatitis increases disease severity and 30-day mortality: COVID PAN collaborative study

Objective: There is emerging evidence that the pancreas may be a target organ of SARS-CoV-2 infection. This aim of this study was to investigate the outcome of patients with acute pancreatitis (AP) and coexistent SARS-CoV-2 infection. Design: A prospective international multicentre cohort study including consecutive patients admitted with AP during the current pandemic was undertaken. Primary outcome measure was severity of AP. Secondary outcome measures were aetiology of AP, intensive care unit (ICU) admission, length of hospital stay, local complications, acute respiratory distress syndrome (ARDS), persistent organ failure and 30-day mortality. Multilevel logistic regression was used to compare the two groups. Results: 1777 patients with AP were included during the study period from 1 March to 23 July 2020. 149 patients (8.3%) had concomitant SARS-CoV-2 infection. Overall, SARS-CoV-2-positive patients were older male patients and more likely to develop severe AP and ARDS (p<0.001). Unadjusted analysis showed that SARS-CoV-2-positive patients with AP were more likely to require ICU admission (OR 5.21, p<0.001), local complications (OR 2.91, p<0.001), persistent organ failure (OR 7.32, p<0.001), prolonged hospital stay (OR 1.89, p<0.001) and a higher 30-day mortality (OR 6.56, p<0.001). Adjusted analysis showed length of stay (OR 1.32, p<0.001), persistent organ failure (OR 2.77, p<0.003) and 30-day mortality (OR 2.41, p<0.04) were significantly higher in SARS-CoV-2 co-infection. Conclusion: Patients with AP and coexistent SARS-CoV-2 infection are at increased risk of severe AP, worse clinical outcomes, prolonged length of hospital stay and high 30-day mortality

Detection of diffuse and specular interface reflections and inter-reflections by color image segmentation

We present a computational model and algorithm for detecting diffuse and specular interface reflections and some inter-reflections. Our color reflection model is based on the dichromatic model for dielectric materials and on a color space, called S space, formed with three orthogonal basis functions. We transform color pixels measured in RGB into the S space and analyze color variations on objects in terms of brightness, hue and saturation which are defined in the S space. When transforming the original RGB data into the S space, we discount the scene illumination color that is estimated using a white reference plate as an active probe. As a result, the color image appears as if the scene illumination is white. Under the whitened illumination, the interface reflection clusters in the S space are all aligned with the brightness direction. The brightness, hue and saturation values exhibit a more direct correspondence to body colors and to diffuse and specular interface reflections, shading, shadows and inter-reflections than the RGB coordinates. We exploit these relationships to segment the color image, and to separate specular and diffuse interface reflections and some inter-reflections from body reflections. The proposed algorithm is effications for uniformly colored dielectric surfaces under singly colored scene illumination. Experimental results conform to our model and algorithm within the liminations discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41303/1/11263_2004_Article_BF00128233.pd

Directional RNA deep sequencing sheds new light on the transcriptional response of Anabaena sp. strain PCC 7120 to combined-nitrogen deprivation

Background: Cyanobacteria are potential sources of renewable chemicals and biofuels and serve as model organisms for bacterial photosynthesis, nitrogen fixation, and responses to environmental changes. Anabaena (Nostoc) sp. strain PCC 7120 (hereafter Anabaena) is a multicellular filamentous cyanobacterium that can "fix" atmospheric nitrogen into ammonia when grown in the absence of a source of combined nitrogen. Because the nitrogenase enzyme is oxygen sensitive, Anabaena forms specialized cells called heterocysts that create a microoxic environment for nitrogen fixation. We have employed directional RNA-seq to map the Anabaena transcriptome during vegetative cell growth and in response to combined-nitrogen deprivation, which induces filaments to undergo heterocyst development. Our data provide an unprecedented view of transcriptional changes in Anabaena filaments during the induction of heterocyst development and transition to diazotrophic growth. Results: Using the Illumina short read platform and a directional RNA-seq protocol, we obtained deep sequencing data for RNA extracted from filaments at 0, 6, 12, and 21 hours after the removal of combined nitrogen. The RNA-seq data provided information on transcript abundance and boundaries for the entire transcriptome. From these data, we detected novel antisense transcripts within the UTRs (untranslated regions) and coding regions of key genes involved in heterocyst development, suggesting that antisense RNAs may be important regulators of the nitrogen response. In addition, many 5' UTRs were longer than anticipated, sometimes extending into upstream open reading frames (ORFs), and operons often showed complex structure and regulation. Finally, many genes that had not been previously identified as being involved in heterocyst development showed regulation, providing new candidates for future studies in this model organism. Conclusions: Directional RNA-seq data were obtained that provide comprehensive mapping of transcript boundaries and abundance for all transcribed RNAs in Anabaena filaments during the response to nitrogen deprivation. We have identified genes and noncoding RNAs that are transcriptionally regulated during heterocyst development. These data provide detailed information on the Anabaena transcriptome as filaments undergo heterocyst development and begin nitrogen fixation