Trade-offs between biomass use and soil cover. The case of rice-based cropping systems in the lake Alaotra region of Madagascar

Farmers in the Lake Alaotra region of Madagascar are currently evaluating a range of conservation agriculture (CA) cropping systems. Most of the expected agroecological functions of CA (weed control, erosion control and water retention) are related to the degree of soil cover. Under farmers’ conditions, the grain and biomass productivity of these systems is highly variable and the biomass is used for several purposes. In this study, we measured biomass production of cover crops and crops in farmers’ fields. Further, we derived relationships to predict the soil cover that can be generated for a particular quantity of mulch. We used these relationships to explore the variability of soil cover that can be generated in farmers’ fields, and to estimate howmuch of the biomass can be removed for use as livestock feed, while retaining sufficient soil cover. Three different kinds of cropping systems were investigated in 91 farmers’ fields. The first two cropping sequences were on the hillsides: (i) maize + pulse (Vigna unguiculata or Dolichos lablab) in year 1, followed by upland rice in year 2; (ii) the second crop sequence included several years of Stylosanthes guianensis followed by upland rice; (iii) the third crop sequence was in lowland paddy fields: Vicia villosa or D. lablab, which was followed by rice within the same year and repeated every year. The biomass available prior to rice sowing varied from 3.6 t ha-1 with S. guianensis to 7.3 t ha-1 with V. villosa. The relationship between the mulch quantity (M) and soil cover (C) was measured using digital imaging and was well described by the following equation: C = 1 - exp(-Am × M), where Am is an area-to-mass ratio with R2 > 0.99 in all cases. The calculated average soil cover varied from 56 to 97% for maize + V. unguiculata and V. villosa, respectively. In order to maintain 90% soil cover at rice sowing, the average amount of biomass of V. villosa that could be removed was at least 3 t ha-1 for three quarters of the fields. This quantity was less for other annual or biennial cropping systems. On average the V. villosa aboveground biomass contained 236 kg N ha-1. The study showed that for the conditions of farmers of Malagasy, the production and conservation of biomass is not always sufficient to fulfil all the above-cited agroecological functions of mulch. Inventory of the soil cover capacity for different types of mulch may help farmers to decide how much biomass they can remove from the fiel

The role of legumes in the sustainable intensification of African smallholder agriculture: Lessons learnt and challenges for the future.

Grain legumes play a key role in smallholder farming systems in sub-Saharan Africa (SSA), in relation to food and nutrition security and income generation. Moreover, because of their N2-fixation capacity, such legumes can also have a positive influence on soil fertility. Notwithstanding many decades of research on the agronomy of grain legumes, their N2-fixation capacity, and their contribution to overall system productivity, several issues remain to be resolved to realize fully the benefits of grain legumes. In this paper we highlight major lessons learnt and expose key knowledge gaps in relation to grain legumes and their contributions to farming system productivity. The symbiosis between legumes and rhizobia forms the basis for its benefits and biological N2-fixation (BNF) relies as much on the legume genotype as on the rhizobial strains. As such, breeding grain legumes for BNF deserves considerably more attention. Even promiscuous varieties usually respond to inoculation, and as African soils contain a huge pool of unexploited biodiversity with potential to contribute elite rhizobial strains, strain selection should go hand-in-hand with legume breeding for N2-fixation. Although inoculated strains can outcompete indigenous strains, our understanding of what constitutes a good competitor is rudimentary, as well as which factors affect the persistence of inoculated rhizobia, which in its turn determines whether a farmer needs to re-inoculate each and every season. Although it is commonly assumed that indigenous rhizobia are better adapted to local conditions than elite strains used in inoculants, there is little evidence that this is the case. The problems of delivering inoculants to smallholders through poorly-developed supply chains in Africa necessitates inoculants based on sterile carriers with long shelf life. Other factors critical for a well-functioning symbiosis are also central to the overall productivity of grain legumes. Good agronomic practices, including the use of phosphorus (P)-containing fertilizer, improve legume yields though responses to inputs are usually very variable. In some situations, a considerable proportion of soils show no response of legumes to applied inputs, often referred to as non-responsive soils. Understanding the causes underlying this phenomenon is limited and hinders the uptake of legume agronomy practices. Grain legumes also contribute to the productivity of farming systems, although such effects are commonly greater in rotational than in intercropping systems. While most cropping systems allow for the integration of legumes, intercropped legumes provide only marginal benefits to associated crops. Important rotational benefits have been shown for most grain legumes though those with the highest N accumulation and lowest N harvest index appear to demonstrate higher residual benefits. N balance estimates often results in contradictory observations, mostly caused by the lack of understanding of belowground contributions of legumes to the N balance. Lastly, the ultimate condition for increased uptake of grain legumes by smallholder farmers lies in the understanding of how legume technologies and management practices can be tailored to the enormous diversity of agroecologies, farming systems, and smallholder farms in SSA. In conclusion, while research on grain legumes has revealed a number of important insights that will guide realization of the full potential of such legumes to the sustainable intensification of smallholder farming systems in SSA, many research challenges remain to be addressed to realize the full potential of BNF in these systems.</p

Exploration of data for analysis using boundary line methodology

The boundary line model has been proposed for interpretation of the plot of a biological response (such as crop yield) against a potentially-limiting variable from observations in a large set of scenarios across which other factors show uncontrolled variation. Under this model the upper bound of the distribution of data represents the limiting effect of the potential factor on the response. Methods have been proposed to fit this model, but we propose that an initial exploratory data analysis step is needed to evaluate evidence that (i) the model is plausible and (ii) that any limiting upper bound is exhibited by the data set (which could, in principle, not include any cases where the factor is limiting). We propose a statistic based on the density of observations in upper sections of early convex hull peels of the data plot. We evaluate this approach using various data sets, some of which have been used for boundary line analysis in previous studies

Structure and gating behavior of the human integral membrane protein VDAC1 in a lipid bilayer

The voltage-dependent anion channel (VDAC), the most abundant protein in the outer mitochondrial membrane, is responsible for the transport of all ions and metabolites into and out of mitochondria. Larger than any of the beta-barrel structures determined to date by magic-angle spinning (MAS) NMR, but smaller than the size limit of cryo-electron microscopy (cryo-EM), VDAC1's 31 kDa size has long been a bottleneck in determining its structure in a near-native lipid bilayer environment. Using a single two-dimensional (2D) crystalline sample of human VDAC1 in lipids, we applied proton-detected fast magic-angle spinning NMR spectroscopy to determine the arrangement of beta strands. Combining these data with long-range restraints from a spin-labeled sample, chemical shift-based secondary structure prediction, and previous MAS NMR and atomic force microscopy (AFM) data, we determined the channel's structure at a 2.2 angstrom root-mean-square deviation (RMSD). The structure, a 19-stranded beta-barrel, with an N-terminal alpha-helix in the pore is in agreement with previous data in detergent, which was questioned due to the potential for the detergent to perturb the protein's functional structure. Using a quintuple mutant implementing the channel's closed state, we found that dynamics are a key element in the protein's gating behavior, as channel closure leads to the destabilization of not only the C-terminal barrel residues but also the alpha 2 helix. We showed that cholesterol, previously shown to reduce the frequency of channel closure, stabilizes the barrel relative to the N-terminal helix. Furthermore, we observed channel closure through steric blockage by a drug shown to selectively bind to the channel, the Bcl2-antisense oligonucleotide G3139

Diversity in perception and management of farming risks in southern Mali

A deeper understanding of how smallholder farmers perceive and manage risks is crucial to identify options that increase farmers' adaptive capacity. We investigated a broad range of risks that play a role in farmers' decision making processes. In the cotton zone of Mali opportunities and constraints vary with the resource endowment of farms. Furthermore, as households are large in this region, often comprising 20–50 family members, intra household diversity may influence perceptions and risk management. For this reason, we analysed diversity both among and within farms. Information was gathered through focus group discussions and a survey with 250 people from 58 households. Risk was assessed as the combination of the perceived frequency of occurrence of hazards and the impact on food availability and income. Farmers faced a diversity of risks, with hazards related to animal and personal health, and climate variability of highest concern. Resource endowment of farms was related to risk perception to a limited extent. Differences within the household were related to the generational factor and decision power, and not to gender. Household members with decision power worried most about risks. Almost a quarter of described hazards occurred with a high frequency and led to a high impact on food availability and income. Low resource-endowed farms were more often exposed to high risks than other farm types. Farmers applied a variety of actions to cope with hazards, yet in many cases farmers lacked a response. Medical actions were targeted to human and animal health hazards. Changes in field and animal management practices, adapted consumption rates and calls on social interactions, were combined for a diversity of hazards. By assessing the diversity of risks encountered by farmers and the diversity of risk management actions taken by farmers, this study goes beyond common risk research that focuses on a single hazard. Our results suggest that development interventions should not focus on either agronomic or economic options separately, but combine both to strengthen social well-being and agricultural production

Is maize-cowpea intercropping a viable option for smallholder farms in the risky environments of semi-arid southern Africa?

Intercropping cereals with legumes can potentially enhance productivity and soil fertility. There is limited experimental evidence on the mechanisms underlying benefits or risks in intercropping systems and below-ground interactions in intercrops remain largely unstudied. Such understanding can inform strategies towards maximising returns to investments, particularly in poor fertility soils on smallholder farms in semi-arid areas of sub-Saharan Africa. Additive intercropping experiments were established covering several seasons (2010/11–2014/15) and different conditions (on-station and on-farm) to determine effects on soil chemical variables, root dynamics and yield of intercrops. Maize was planted with the first effective rains and received either no fertiliser or 40 kg N ha−1. Cowpea was planted on the same date as maize or three weeks after planting maize in intercrops or sole stands and received no fertiliser. End-of-season available N was highest (P 1. Intercropping, however, resulted in compromised cowpea yields especially under the relay intercrop compared with the sole cowpea stands whilst maize yield was either not affected or improved. We attributed this to the lack of below-ground niche differentiation in root distribution between maize and cowpea. Maize–cowpea intercropping with low doses of N fertiliser resulted in over-yielding compared with monocropping. Intercropping proved to be a robust option across seasons and soil types, confirming that it is a promising option for resource-poor smallholders

Projective representation of k-Galilei group

The projective representations of k-Galilei group G_k are found by contracting the relevant representations of k-Poincare group. The projective multiplier is found. It is shown that it is not possible to replace the projective representations of G_k by vector representations of some its extension.Comment: 15 pages Latex fil

Tillage, mulch and fertiliser impacts on soil nitrogen availability and maize production in semi-arid Zimbabwe

Conservation agriculture has been promoted widely in sub-Saharan African to cushion smallholder farmers against the adverse effects of soil fertility decline, stabilize crop yields and increase resilience to climate change and variability. Our study aimed to determine if aspects of CA, namely tillage and mulching with manure and fertiliser application, improved soil mineral N release, plant N uptake and maize yields in cropping systems on poor soils in semi-arid Matobo, Zimbabwe. The experiment, run for three seasons (2012/13–2014/15), was a split-split plot design with three replicates. Tillage (animal-drawn ploughing and ripping) was the main plot treatment and residue application was the sub plot treatment with two levels (100% residues removed or retained after harvest). Five fertility amendments (mineral fertiliser at 0, 20 and 40 kg N ha-1, 5 t ha-1 manure only and 5 t ha-1 manure + 20 kg N ha-1) were sub-sub plot treatments. Plough tillage stimulated N mineralisation by 4–19 kg N ha-1 and maize N uptake 13–23% more than the ripper tillage. When mulch was added to the plough tillage, mineralisation was slowed resulting in less crop N uptake (by 5–19%) compared with no mulch application. N uptake was highest in the manure treatments. N recovery and agronomic N efficiency by maize were highly variable over the three seasons, reflecting the uncertainty complicating farmers’ decision making. Nitrogen recovery in the manure treatments was generally poor in the first season resulting in low grain yields in the range 100–260 kg ha-1 regardless of tillage, though higher in subsequent seasons. In the second season manure application gave the largest grain yields under the ripper tillage, both with and without mulch averaging 1850 and 2228 kg ha-1 respectively. Under the plough tillage, the 40 kg N ha-1 treatment gave the highest grain yields of 1985 kg ha-1. In the third season yields were generally poor under all treatments due to low and poorly-distributed rainfall. The CA principles of minimum soil disturbance and maintenance of a permanent mulch cover resulted in reduced soil mineral N availability for crop uptake and poor maize yields. Nutrient inputs through mineral fertilisers and manure are key to ensuring production in such infertile, sandy soils which predominate in semi-arid regions of southern Africa

A recipe for success? Learning from the rapid adoption of improved chickpea varieties in Ethiopia

Many studies detail constraints deemed responsible for the limited adoption of new technologies among smallholder farmers in sub-Saharan Africa. By contrast, here we study the conditions that led to the remarkably fast spread of improved chickpea varieties in Ethiopia. Within just seven years, the adoption rate rose from 30 to 80% of the farmers. A combination of factors explains the rapid uptake. Their attraction lay in superior returns and disease resistance. Chickpea was already an important crop for rural households in the studied districts, for both cash income and consumption. Good market access and an easy accessibility of extension services advanced the adoption process. Thus, an attractive technology suitable for rural households in a conducive environment enabled adoption. Our findings prompt us to stress the importance of tailoring agricultural innovations to the realities and demands of rural households, and the need to design and deploy interventions on the basis of ex-ante knowledge on factors potentially determining their success or failure

Risk management options in maize cropping systems in semi-arid areas of Southern Africa

Although rainfed cropping in semi-arid areas is risky due to frequent droughts and dry spells, planting early with the first rains is often expected to result in yield benefits. We hypothesised that planting early leads to yield benefits if the planting coincides with a mineral N flush at the start of the season but leads to crop failure if there is a false start to the cropping season. The effects of different management options, including tillage (ploughing and ripping), mulch (two levels 0 and 2 t ha−1) and fertility amendments (five levels: 0; 20 and 40 kg N ha−1; 5 t manure ha−1 and 5 t ha−1 manure + 20 kg N ha−1) on grain yields were simulated using the calibrated and tested APSIM model over a 30-year period (1984–2015). Yields were simulated and compared across seven planting date scenarios (1 November, 15 November, 30 November, 15 December, 31 December, 15 January and planting when cumulative rainfall of 20 mm was received in three consecutive days). Planting with the first rains with manure + 20 kg N ha−1 resulted in the best average yield of 2271 kg ha−1 whilst the poorest average yields of 22 kg ha−1 were observed with planting on 15 January with no fertility amendment (0 kg N ha−1). Planting early (1 Nov to 15 Nov) and with the first rains resulted in exceeding the food self-sufficiency threshold of 1080 kg ha-1 in 40–83 % of the cases if fertility amendments are applied, as well as a low probability of complete crop failure, ranging from 0 to 40%. Grain yield penalties due to a false start followed the trend: ripper + mulch > plough + mulch > ripper (no mulch) averaging 256, 190 and 182 kg ha-1 respectively across all the fertility treatments. The model was able to simulate the occurrence of the mineral N flush with the first rains. Its coincidence with planting resulted in average yield benefits of 712, 452, 382 and 210 kg ha-1 for the following respective planting dates: 1 Nov, 15 Nov, 30 Nov, variable date when >20 mm rainfall was received. Early planting, in combination with reduced tillage, mulch and N containing fertility amendments is critical to reduce risk of crop failure in the smallholder cropping systems of semi-arid areas of southern Africa and achieve the best possible yields