Composicao das especies de moscas-varejeiras do lixo no norte da Tailandia: avaliacao da altitude

Distribution and occurrence of blow flies of forensic importance was performed during 2007 and 2008 in Chiang Mai and Lampang Provinces, northern Thailand. Surveys were conducted in forested areas for 30 minutes using a sweep net to collected flies attracted to a bait. A total of 2,115 blow flies belonging to six genera and 14 species were collected; Chrysomya megacephala (Fabricius) (44.7%), C. pinguis (Walker) (15.1%), C. chani Kurahashi (9.3%), C. thanomthini Kurahashi & Tumrasvin (0.3%); Achoetandrus rufifacies (Macquart) (10.5%), A. villeneuvi (Patton) (2.2%); Lucilia papuensis Macquart (2.2%), L. porphyrina (Walker) (12.4%), L. sinensis Aubertin (0.7%); Hemipyrellia ligurriens (Wiedemann) (1.3%), H. pulchra (Wiedemann) (0.1%); Hypopygiopsis infumata (Bigot) (0.6%), Hy. tumrasvini Kurahashi (0.2%) and Ceylonomyia nigripes Aubertin (0.4%). Among them, C. megacephala was the predominant species collected, particularly in the summer. The species likely to prevail in highland areas are C. pinguis, C. thanomthini, Hy. tumrasvini, L. papuensis and L. porphyrina.Distribuição e ocorrência de moscas-varejeiras de importância forense foi realizada durante 2007 e 2008 nas províncias de Chiang Mai e Lampang, norte da Tailândia. Os levantamentos foram feitos em áreas de florestas, durante 30 minutos usando rede de varredura para coletar as moscas atraídas por iscas. Um total de 2115 moscas-varejeiras pertencentes a seis gêneros e 14 espécies foram coletados; Chrysomya megacephala (Fabricius) (44,7%), C. pinguis (Walker) (15,1%), C. chani Kurahashi (9,3%), C. thanomthini Kurahashi & Tumrasvin (0,3%); Achoetandrus rufifacies (Macquart) (10,5%), A. villeneuvi (Patton) (2,2%); Lucilia papuensis Macquart (2,2%), L. porphyrina (Walker) (12,4%), L. sinensis Aubertin (0,7%); Hemipyrellia ligurriens (Wiedemann) (1,3%), H. pulchra (Wiedemann) (0,1%); Hypopygiopsis infumata (Bigot) (0,6%), Hy. tumrasvini Kurahashi (0,2%) e Ceylonomyia nigripes Aubertin (0,4%). Dentre elas a C. megacephala foi a espécie predominante coletada particularmente no verão. As espécies capazes de predominar nas áreas altas são: C. pinguis, C. thanomthini, Hy. tumrasvini, L. papuensis e L. porphyrina

Confocal laser scanning microscopy as a valuable tool in Diptera larval morphology studies

Larval morphology of flies is traditionally studied using light microscopy, yet in the case of fine structures compound light microscopy is limited due to problems of resolution, illumination and depth of field, not allowing for precise recognition of sclerites’ edges and interactions. Using larval instars of cyclorrhaphan Diptera, we show the usefulness of confocal laser scanning microscopy (CLSM) for studying the morphological characters of immature stages by taking advantage of the autofluorescent properties of cephaloskeleton structures. We compare data obtained from killed but unprepared larvae with those from larvae prepared by clearing according to two commonly used methods, either with potassium hydroxide or with Hoyer’s medium. We also evaluated the CLSM application for examining already slide-mounted larvae stored in museum collections and those freshly prepared. Our results indicate that CLSM and 3D reconstruction are excellent for visualizing small, compound structures of cylrorrhaphan larvae cephaloskeleton, if appropriate clearing techniques, i.e. the application of KOH, are used. Maximum intensity projection of confocal data sets obtained from material freshly prepared and that stored in museum collection does not differ. Because of this and the fact that KOH is commonly used as a clearing method to examine the cephaloskeleton of Diptera larvae, it is possible, and highly recommended, to use slides already prepared with this method for re-examination by CLSM. We conclude that CLSM application can be an invaluable source of data for studies of larval morphology of Cyclorrhapha by way of taxonomic diagnoses, character identification and improvement in characters homologization.This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited

Fishborne Zoonotic Intestinal Trematodes, Vietnam

These parasites are an unrecognized food safety risk in a population with a tradition of eating raw fish

The uses of Chrysomya megacephala (Fabricius, 1794)(Diptera: Calliphoridae) in forensic entomology:

Chrysomya megacephala (Fabricius, 1794) occurs on every continent and is closely associated with carrion and decaying material in human environments. Its abilities to find dead bodies and carry pathogens give it a prominence in human affairs that may involve prosecution or litigation, and therefore forensic entomologists. The identification, geographical distribution and biology of the species are reviewed to provide a background for approaches that four branches of forensic entomology (urban, stored-product, medico-criminal and environmental) might take to investigations involving this fly

Ultrastructure of adhesive device in fly in families Calliphoridae, Muscidae and Sarcophagidae, and their implication as mechanical carriers of pathogens

Abstract The ultrastructure of adhesive device or the pulvilli, pad-like structure between the tarsal claws of the legs, is presented in the blowfly (Calliphoridae), housefly and relatives (Muscidae), and flesh fly (Sarcophagidae) through scanning electron microscopy. The blowfly species were Chrysomya chani, Chrysomya nigripes, Chrysomya pinguis, and Chrysomya villeneuvi; while those of the housefly and relatives were Musca domestica and Hydrotaea chalcogaster, respectively. Fresh fly species included Boettcherisca peregrina and Liosarcophaga dux. Numerous tenent setae were observed on the ventral side of the pulvilli. Two features of the tip of the tenent setae were found as a spatula-like (in C. chani, C. pinguis, C. nigripes, M. domestica, H. chalcogaster, B. peregrina, and L. dux) and spoon-like tip (in C. villeneuvi). Transmission electron microscopy of the tenent setae in M. domestica revealed the electron-lucent centrally located, suggesting an adhesive substance. These results provided anatomical information that allow us to not only understand the successful attachment of flies to smooth surfaces but also clarify their role as a mechanical carrier of microorganisms