Cannabinoid Receptor Involvement in Stress-Induced Cocaine Reinstatement: Potential Interaction with Noradrenergic Pathways

This study examined the role of endocannabinoid signaling in stress-induced reinstatement of cocaine seeking and explored the interaction between noradrenergic and endocannabinergic systems in the process. A well-validated preclinical model for human relapse, the rodent conditioned place preference assay, was used. Cocaine-induced place preference was established in C57BL/6 mice using injections of 15 mg/kg cocaine. Following extinction of preference for the cocaine-paired environment, reinstatement of place preference was determined following 6 min of swim stress or cocaine injection (15 mg/kg, i.p.). The role of endocannabinoid signaling was studied using the cannabinoid antagonist AM-251 (3 mg/kg, i.p.). Another cohort of mice was tested for reinstatement following administration of the cannabinoid agonist CP 55,940 (10, 20, or 40 μg/kg, i.p.). The alpha-2 adrenergic antagonist BRL-44408 (5 mg/kg, i.p.) with or without CP 55,940 (20 μg/kg) was administered to a third group of mice. We found that: (1) AM-251 blocked forced swim-induced, but not cocaine-induced, reinstatement of cocaine-seeking behavior; (2) the cannabinoid agonist CP 55,940 did not reinstate cocaine-seeking behavior when administered alone but did synergize with a non-reinstating dose of the alpha-2 adrenergic antagonist BRL-44408 to cause reinstatement. These results are consistent with the hypothesis that stress exposure triggers the endogenous activation of CB1 receptors and that activation of the endocannabinoid system is required for the stress-induced relapse of the mice to cocaine seeking. Further, the data suggest that the endocannabinoid system interacts with noradrenergic mechanisms to influence stress-induced reinstatement of cocaine-seeking behavior

Rearrangement of secondary flow over spanwise heterogeneous roughness

Turbulent flow over a surface with streamwise-elongated rough and smooth stripes is studied by means of direct numerical simulation (DNS) in a periodic plane open channel with fully resolved roughness. The goal is to understand how the mean height of roughness affects the characteristics of the secondary flow formed above a spanwise heterogeneous rough surface. To this end, while the statistical properties of roughness texture as well as the width and spacing of the rough stripes are kept constant, the elevation of the smooth stripes is systematically varied in different simulation cases. Utilizing this variation, three configurations – representing protruding, recessed and an intermediate type of roughness – are analysed. In all cases, secondary flows are present and the skin friction coefficients calculated for all the heterogeneous rough surfaces are meaningfully larger than what would result from the area-weighted average of those of homogeneous smooth and rough surfaces. This drag increase appears to be linked to the strength of the secondary flow. The rotational direction of the secondary motion is shown to depend on the relative surface elevation. The present results suggest that this rearrangement of the secondary flow is linked to the spatial distribution of the spanwise-wall-normal Reynolds stress component, which carries opposing signs for protruding and recessed roughness

Modelling spanwise heterogeneous roughness through a parametric forcing approach

Inhomogeneous rough surfaces in which strips of roughness alternate with smooth-wall strips are known to generate large-scale secondary motions. Those secondary motions are strongest if the strip width is of the order of the half-channel height and they generate a spatial wall shear stress distribution whose mean value can significantly exceed the area-averaged mean value of a homogeneously smooth and rough surface. In the present paper it is shown that a parametric forcing approach (Busse & Sandham, J. Fluid Mech., vol. 712, 2012, pp. 169–202; Forooghi et al., Intl J. Heat Fluid Flow, vol. 71, 2018, pp. 200–209), calibrated with data from turbulent channel flows over homogeneous roughness, can capture the topological features of the secondary motion over protruding and recessed roughness strips (Stroh et al., J. Fluid Mech., vol. 885, 2020, R5). However, the results suggest that the parametric forcing approach roughness model induces a slightly larger wall offset when applied to the present heterogeneous rough-wall conditions. Contrary to roughness-resolving simulations, where a significantly higher resolution is required to capture roughness geometry, the parametric forcing approach can be applied with usual smooth-wall direct numerical simulation resolution resulting in less computationally expensive simulations for the study of localized roughness effects. Such roughness model simulations are employed to systematically investigate the effect of the relative roughness protrusion on the physical mechanism of secondary flow formation and the related drag increase. It is found that strong secondary motions present over spanwise heterogeneous roughness with geometrical height difference generally lead to a drag increase. However, the physical mechanism guiding the secondary flow formation, and the resulting secondary flow topology, is different for protruding roughness strips and recessed roughness strips separated by protruding smooth surface strips

Variability of Cu 2 ZnSnS 4 nanoparticle hot injection synthesis and modifications by thin film annealing

As a quaternary semiconductor with a direct energy bandgap of around 1.4 eV, Cu2ZnSnS4 is a promising candidate for absorber layers in next generation thin-film solar PV devices. It has the advantage of being based on low cost earth-abundant elements. Solution based synthesis approaches show the greatest potential for scaling up manufacture. Cu2ZnSnS4 devices are currently limited in efficiency because of a large open circuit voltage deficit, arising predominantly from high concentrations of point defects and charge compensation defect complexes. To drive device efficiency robust, reliable and reproducible synthesis protocols are required. We have produced a series of Cu2ZnSnS4 thin films by spin coating nanoparticle ink suspensions fabricated under nominally identical conditions to investigate the inherent variability in hot injection synthesis of Cu2ZnSnS4 nanoparticles by fabricating 11 batches using the same initial conditions. We use two different chemical routes to extratct nanoparticles from solution after synthesis. We find that the lattice constants of the nanocrystalline material do not change significantly. The relative concentration of the constituent elements varies with S having the largest anion variation of ±3.8% as compared to metal cation variations of Zn ±2.4%, Cu ±1.8%, and Sn ±1.4% with Zn having the largest cation variation. We compare data from energy dispersive X-ray (EDX) and inductively coupled plasma mass spectroscopy (ICPMS) chemical analysis methods and find that the ICPMS analysis has a consistently smaller standard deviation, an average of 0.1 lower, as this technique samples a large volume of material. We observe variation in the kesterite tetragonal lattice constants a and c, and energy bandgap Eg across the different samples, although there is no systematic change in the chemical composition. The average bandgap of as-synthesised films is 1.14 eV. We find that annealing in a sulphur rich environment has no systematic impact on the Cu/(Zn + Sn) cation ratio and leads to a decrease of −0.4 in the Zn/Sn ratio. At higher annealing temperatures, 500–600 °C, the bandgap shows a linear increase of +0.15 eV accompanied by the formation of abnormal grains and an increase in the size of the crystalline scattering domain τ, determined from the X-ray spectra, from 30–100 nm. The most dramatic changes occur in the first 0.5 hours of annealing. These findings will help in the design of fabrication strategies for higher efficiency Cu2ZnSnS4 photovoltaic devices

A Comparative Analysis of Soccer Skill Tests on Varying Experience Levels

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A New Point of View on Skin-Friction Contributions in Adverse-Pressure-gradient Turbulent Boundary Layers

Skin-friction decompositions such as the so-called FIK identity (Fukagata et al., 2002) are useful tools in identifying relevant contributions to the friction, but may also lead to results difficult to interpret when the total friction is recovered from cancellation of multiple terms with large values. We propose a new formulation of the FIK contributions related to streamwise inhomogeneity, which is derived from the convective form of the momentum equation and using the concept of dynamic pressure. We examine turbulent boundary layers subjected to various pressure-gradient conditions, including cases with drag-reducing control. The new formulation distinguishes more precisely the roles of the free-stream pressure distribution, wall-normal convection, and turbulent fluctuations. Our results allow to identify different regimes in adverse-pressure-gradient turbulent boundary layers, corresponding to different proportions of the various contributions, and suggest a possible direction towards studying the onset of mean separation

Comparison of Different Solvers and Geometry Representation Strategies for Dns of Rough Wall Channel Flow

In the present study we investigate an incompressible turbulent channel flow with heat transfer at Reτ = 180 with a deterministic surface topography consisting of truncated cones. Two solvers for each of the two boundary handling strategies are considered. With Nek5000 and OpenFOAM the influence of the roughness elements is directly accounted for by an unstructured body fitted mesh, whereas Xcompact3d and SIMSON utilize the immersed boundary method (IBM) to deal with the 3D geometry. The main focus of this work is on an evaluation of the usability of the IBM and a comparison of the parallel performance of the different solvers. Since usability is an ambiguous definition, various quantities are compared: global statistics like Nusselt number and friction coefficient, one-dimensional wall-normal profiles for first and second order statistics, as well as three-dimensional averages over roughness sections. In addition, the computational effort for each method is documented

Primary and secondary electrical space power based on advanced PEM systems

For new space ventures, power continues to be a pacing function for mission planning and experiment endurance. Although electrochemical power is a well demonstrated space power technology, current hardware limitations impact future mission viability. In order to document and augment electrochemical technology, a series of experiments for the National Aeronautics and Space Administration Lewis Research Center (NASA LeRC) are underway at the Los Alamos National Laboratory that define operational parameters on contemporary proton exchange membrane (PEM) hardware operating with hydrogen and oxygen reactants. Because of the high efficiency possible for water electrolysis, this hardware is also thought part of a secondary battery design built around stored reactants - the so-called regenerative fuel cell. An overview of stack testing at Los Alamos and of analyses related to regenerative fuel cell systems are provided in this paper. Finally, this paper describes work looking at innovative concepts that remove complexity from stack hardware with the specific intent of higher system reliability. This new concept offers the potential for unprecedented electrochemical power system energy densities