Characterization of Tef (Eragrostis tef zucc. Trotter [cv. Magna]) in Minjar Shenkora district of central Ethiopia

Tef (Eragrostis tef Zucc. Trotter) is a major cereal crop in Ethiopia and performs well in altitudes ranging from 1700 to 2400 m above sea level. Tef “cv. Magna” exhibits high variability in quality and productivity per unit area in different parts of the country, hence it gives different market prices accordingly. This variety, also known as “Minjar magna Tef’” in the study area, is intensely preferred by the consumers than produced elsewhere thought the country. Thus, participatory variety characterization trial was conducted in 2015 and 2016 in Minjar Shenkora district of central Ethiopia to characterize Tef “cv. Magna” variety so as to obtain and create a primary platform for the branding of the variety in the future. Forty-five (45) farmers were selected purposively for the study atnine Tef “cv. Magna” producing sub-districts: Arerti, Agirat, Ameti, Adama, Bolo Giorgis, Bolo Silase, Chele, Kombolcha and Korma. A quadrant (1m x 1m) was used by throwing at five different locations per farmer field, and ten plants were randomly selected as experimental plants. Results revealed that the mean days were found to be 6.78, 42.44 and 100.89 for 50% emergence, 50% heading, and 50% maturity, respectively. The results of the present study show that the mean plant height and panicle length of the crop were 1.24m and 0.46m, respectively. The average tiller number, weight of main panicle and main panicle seed weight of Tef “cv. Magna” was found to be 4.49, 1.58 gm and 1.03 gm, respectively. Furthermore, the mean yield and shoot biomass were found to be 19.49 Qt/ha and 123 Qt/ha, respectively. The proximate compositions of Tef “cv. Magna” were 11.09% moisture content, 2.49% ash content, 74.07% carbohydrate, 1.75% crude fiber, 9.08% crude protein, 3.27% crude fat and 361.98 Kcal/mole energy. The mineral contents of Tef “cv. Magna” was Na: 49.99, K: 5686.54, P: 2935.23, Fe: 115.79, Cu: 6.36, Zn: 26.22, Ca: 1741.24 and Mg: 1427.08 mg/Kg. Though characterization of the variety at the molecular level and comparative study with different varieties of the crop should be done in the near future, our study forms a primary platform for further activities to give a brand name for Tef “cv. Magna”.Key words: Brand, Characterization, Minerals, Proximate composition, Tef ‘cv. Magna

Level of compassionate health care service provision and its associated factors among health professionals working in public hospitals of Addis Ababa: health professionals' perspective

Background: Compassionate health care service is important for good clinical outcomes and patient satisfaction. However, complaints of non-compassionate care became very familiar and popular grievance of the community in the health care system. The aim of this study was to assess the level of compassionate health care service provision and its associated factors among health professionals working in public hospitals of Addis Ababa, Ethiopia. Methods: A facility based cross sectional study was conducted among six public hospitals of Addis Ababa, Ethiopia. Simple random sampling and systematic sampling methods were applied to select hospitals and each study participants respectively. Data were collected from 400 participants using a self-administered structured questionnaire. The mean of compassionate health care was taken as the cut point to label respondent as good and poor compassionate health care service provider. Bivariate and multivariable logistic regressions were done to determine the associated factors for compassionate care provision. Variables with p values < 0.05 at the multivariable analysis were considered as significantly associated with compassionate care provision. Results: Only 48.0% of health care professionals provided good compassionate care. Learned to be compassionate (AOR ¼ 5.083; CI 95% 2.69, 9.58); patient and their family realistic expectation (AOR ¼ 2.24; CI 95% 1.07, 4.66) were found to be significantly associated with good compassionate health care provision. Conclusions: and recommendations: Good compassionate health care service provision in Addis Ababa hospitals was very low. Teaching health science and medical students to be compassionate and ensuring health care clients to have realistic expectation may be important to farther enhance compassionate health care service provision.Mulugeta Abate, Nigussie Tadesse, Kindie Mitik

Expression of the transcription factor, TFII-I, during post-implantation mouse embryonic development

<p>Abstract</p> <p>Background</p> <p>General transcription factor (TFII-I) is a multi-functional transcription factor encoded by the Gtf2i gene, that has been demonstrated to regulate transcription of genes critical for development. Because of the broad range of genes regulated by TFII-I as well as its potential role in a significant neuro-developmental disorder, developing a comprehensive expression profile is critical to the study of this transcription factor. We sought to define the timing and pattern of expression of TFII-I in post-implantation embryos at a time during which many putative TFII-I target genes are expressed.</p> <p>Findings</p> <p>Antibodies to the N-terminus of TFII-I were used to probe embryonic mouse sections. TFII-I protein is widely expressed in the developing embryo. TFII-I is expressed throughout the period from E8-E16. However, within this period there are striking shifts in localization from cytoplasmic predominant to nuclear. TFII-I expression varies in both a spatial and temporal fashion. There is extensive expression in neural precursors at E8. This expression persists at later stages. TFII-I is expressed in developing lung, heart and gut structures. There is no evidence of isoform specific expression. Available data regarding expression patterns at both an RNA and protein level throughout development are also comprehensively reviewed.</p> <p>Conclusions</p> <p>Our immunohistochemical studies of the temporal and spatial expression patterns of TFII-I in mouse embryonic sections are consistent with the hypothesis that hemizygous deletion of <it>GTF2I </it>in individuals with Williams-Beuren Syndrome contributes to the distinct cognitive and physiological symptoms associated with the disorder.</p

Development of a prototype clinical decision support tool for osteoporosis disease management: a qualitative study of focus groups

<p>Abstract</p> <p>Background</p> <p>Osteoporosis affects over 200 million people worldwide, and represents a significant cost burden. Although guidelines are available for best practice in osteoporosis, evidence indicates that patients are not receiving appropriate diagnostic testing or treatment according to guidelines. The use of clinical decision support systems (CDSSs) may be one solution because they can facilitate knowledge translation by providing high-quality evidence at the point of care. Findings from a systematic review of osteoporosis interventions and consultation with clinical and human factors engineering experts were used to develop a conceptual model of an osteoporosis tool. We conducted a qualitative study of focus groups to better understand physicians' perceptions of CDSSs and to transform the conceptual osteoporosis tool into a functional prototype that can support clinical decision making in osteoporosis disease management at the point of care.</p> <p>Methods</p> <p>The conceptual design of the osteoporosis tool was tested in 4 progressive focus groups with family physicians and general internists. An iterative strategy was used to qualitatively explore the experiences of physicians with CDSSs; and to find out what features, functions, and evidence should be included in a working prototype. Focus groups were conducted using a semi-structured interview guide using an iterative process where results of the first focus group informed changes to the questions for subsequent focus groups and to the conceptual tool design. Transcripts were transcribed verbatim and analyzed using grounded theory methodology.</p> <p>Results</p> <p>Of the 3 broad categories of themes that were identified, major barriers related to the accuracy and feasibility of extracting bone mineral density test results and medications from the risk assessment questionnaire; using an electronic input device such as a Tablet PC in the waiting room; and the importance of including well-balanced information in the patient education component of the osteoporosis tool. Suggestions for modifying the tool included the addition of a percentile graph showing patients' 10-year risk for osteoporosis or fractures, and ensuring that the tool takes no more than 5 minutes to complete.</p> <p>Conclusions</p> <p>Focus group data revealed the facilitators and barriers to using the osteoporosis tool at the point of care so that it can be optimized to aid physicians in their clinical decision making.</p