Variation of abductor pollicis longus tendons in cadavers

Background: First extensor compartment of the wrist comprises of abductor pollicis longus and extensor pollicis brevis. It helps in movement and stabilization of thumb. Variations in the number of tendons of APL muscle may be asymptomatic and often incidental finding. Stenosing tenosynovitis of the first dorsal compartment of the wrist or de Quervains disease is a commonly encountered debilitating condition of the wrist.Methods: This cadaveric study was done on 40 forearms in 20 cadavers available in Department of anatomy and forensic medicine at our institute. The muscles of extensor compartments were dissected, extensor retinaculum split over first extensor compartment, tendons of APL exposed. Study period from February-2017 to February-2018.Results: There were 6 female and 14 male cadavers. The APL muscle was found with a single tendon in 2, double in 30, triple in 8. There were variations in the insertion of the APL tendon as well. In all hands, the APL tendon had insertion into the first metacarpal bone and in 20 hands (50%), it had second insertion into the trapezium.Conclusions: Variation of APL muscle insertion in the Indian population and two or more tendinous slips attached commonly to the first metacarpal base and the trapezium may be the cause of treatment failure in DQT and cause of Trapeziometacarpal arthritis. Further studies needs to be done for further evaluation

Comparative Root Transcriptomics Provide Insights into Drought Adaptation Strategies in Chickpea (Cicer arietinum L.)

Drought adversely affects crop production across the globe. The root system immensely contributes to water management and the adaptability of plants to drought stress. In this study, drought-inducedphenotypic andtranscriptomic responses of two contrasting chickpea (Cicer arietinum L.) genotypes were compared at the vegetative, reproductive transition, and reproductive stages. At the vegetative stage, drought-tolerant genotype maintained higher root biomass, length, and surface area under drought stress as compared to sensitive genotype. However, at the reproductive stage, root length and surface area of tolerant genotype was lower but displayed higher root diameter than sensitive genotype. The shoot biomass of tolerant genotype was overall higher than the sensitive genotype under drought stress. RNA-seq analysis identified genotype- and developmental-stage specific differentially expressed genes (DEGs) in response to drought stress. At the vegetative stage, a total of 2161 and 1873 DEGs, and at reproductive stage 4109 and 3772 DEGs, were identified in the tolerant and sensitive genotypes, respectively. Gene ontology (GO) analysis revealed enrichment of biological categories related to cellular process, metabolic process, response to stimulus, response to abiotic stress, and response to hormones. Interestingly, the expression of stress-responsive transcription factors, kinases, ROS signaling and scavenging, transporters, root nodulation, and oxylipin biosynthesis genes were robustly upregulated in the tolerant genotype, possibly contributing to drought adaptation. Furthermore, activation/repression of hormone signaling and biosynthesis genes was observed. Overall, this study sheds new insights on drought tolerance mechanisms operating in roots with broader implications for chickpea improvement

Fingerprinting the Asterid Species Using Subtracted Diversity Array Reveals Novel Species-Specific Sequences

Background: Asterids is one of the major plant clades comprising of many commercially important medicinal species. One of the major concerns in medicinal plant industry is adulteration/contamination resulting from misidentification of herbal plants

Geoelectrical response of a water bearing sandstone shale sequence and estimation of sand shale ratio

An attempt has been made to use the geoelectrical response of a water bearing sandstone-shale sequence to estimate the sandstone-shale ratio. In all, 168 sandstone-shale sequence models were generated using Monte-Carlo simulation technique. The resistivity transform and its autocorrelation function of all the models were calculated. The amplitude, phase, power and log spectra Were also extracted. The power spectrum appears to hold promise in estimating the sand stone-shale ratio of these models because the power amplitude P2 shows a high negative correlation with sandstone shale ratio values

Trehalose: effect on cryopreservation of umbilical cord blood-derived hematopoietic stem cells

Introduction: Concerns over dimethyl sulfoxide (DMSO) toxicity, related to adverse reactions after hematopoietic stem cells (HSCs) therapy, warrant the development of an optimized DMSO-free or DMSO-reduced cryopreservation protocol for the quality and safety of HSCs. In this regard, the ideal concentration of trehalose, as a non-toxic natural cryoprotectant, is still an area of research. Based on the outcome of our previous study on lower concentrations of trehalose, the present study was focused on evaluating its cryoprotective efficacy at an increased concentration (0.5 M) on HSCs compared to 10% DMSO. This is a laboratory-based experimental study. Material and methods: The separated mononuclear cells collected from umbilical cord blood were set for culture up to two passages to get HSCs. The two different concentrations of trehalose, with and without 5% DMSO, were considered as freezing media for the preservation of the harvested HSCs in a slow freezing set up. Two sequential functional assays, viability followed by hematopoietic colony-forming unit assay, were performed with post-thawed cells of freezing media used in this study. Seventeen cord blood samples were selected. Results: Study results revealed 0.5 M trehalose and DMSO 5% showed the highest viability of 91.8 ±2.8% of HSCs. 5% DMSO inclusion to trehalose (0.5 M) ameliorated hematopoietic colonies such as erythroid and myeloid colonies with no significant difference from that of 10% DMSO. Conclusion: 0.5 M trehalose has proved to be a better concentration than 10% DMSO alone. This experimental study needs further transplantation-based clinical trials using post-thawed cells to ensure the safety of preserved HSCs from cord blood and other sources

Enhanced group-based wireless ad-hoc sensor network protocol

[EN] Communication is the major energy consumption source in wireless ad-hoc sensor networks. Thus, an efficient tradeoff between the energy cost of the communication and network's performance is a key challenge in conceiving a wireless ad-hoc sensor network. In this article, we propose an improved group-based architecture for wireless ad-hoc sensor networks. An optimized group forming procedure and an efficient communication operation are introduced. In order to validate the proposed approach, we suggest a group-based strategy to monitor pharmaceutical drugs during transportation. Real measurements of temperature and vibration were performed to validate the effectiveness of our approach.Khedher, M.; Lloret, J.; Douik, A. (2016). Enhanced group-based wireless ad-hoc sensor network protocol. International Journal of Distributed Sensor Networks. 12(7):1-18. https://doi.org/10.1177/1550147716659427S118127Dargie, W., & Poellabauer, C. (2010). Fundamentals of Wireless Sensor Networks. doi:10.1002/9780470666388Singh, S. P., & Sharma, S. C. (2015). A Survey on Cluster Based Routing Protocols in Wireless Sensor Networks. Procedia Computer Science, 45, 687-695. doi:10.1016/j.procs.2015.03.133Liao, Y., Qi, H., & Li, W. (2013). Load-Balanced Clustering Algorithm With Distributed Self-Organization for Wireless Sensor Networks. IEEE Sensors Journal, 13(5), 1498-1506. doi:10.1109/jsen.2012.2227704Peng, I.-H., & Chen, Y.-W. (2013). Energy consumption bounds analysis and its applications for grid based wireless sensor networks. Journal of Network and Computer Applications, 36(1), 444-451. doi:10.1016/j.jnca.2012.04.014Lloret, J., Garcia, M., Tomás, J., & Boronat, F. (2008). GBP-WAHSN: A Group-Based Protocol for Large Wireless Ad Hoc and Sensor Networks. Journal of Computer Science and Technology, 23(3), 461-480. doi:10.1007/s11390-008-9147-6Lloret, J., García, M., Boronat, F., & Tomás, J. (s. f.). MANET Protocols Performance in Group-based Networks. IFIP International Federation for Information Processing, 161-172. doi:10.1007/978-0-387-84839-6_13Lloret, J., Garcia, M., & Tomas, J. (s. f.). Improving Mobile and Ad-hoc Networks performance using Group-Based Topologies. Wireless Sensor and Actor Networks II, 209-220. doi:10.1007/978-0-387-09441-0_18Lloret, J., Palau, C., Boronat, F., & Tomas, J. (2008). Improving networks using group-based topologies. Computer Communications, 31(14), 3438-3450. doi:10.1016/j.comcom.2008.05.030Garcia, M., Sendra, S., Lloret, J., & Canovas, A. (2011). Saving energy and improving communications using cooperative group-based Wireless Sensor Networks. Telecommunication Systems, 52(4), 2489-2502. doi:10.1007/s11235-011-9568-3Garcia, M., & Lloret, J. (2009). A Cooperative Group-Based Sensor Network for Environmental Monitoring. Cooperative Design, Visualization, and Engineering, 276-279. doi:10.1007/978-3-642-04265-2_41Shaikh, R. A., Jameel, H., d’ Auriol, B. J., Heejo Lee, Sungyoung Lee, & Young-Jae Song. (2009). Group-Based Trust Management Scheme for Clustered Wireless Sensor Networks. IEEE Transactions on Parallel and Distributed Systems, 20(11), 1698-1712. doi:10.1109/tpds.2008.258Chen, Y.-S., Hsu, C.-S., & Lee, H.-K. (2014). An Enhanced Group Mobility Protocol for 6LoWPAN-Based Wireless Body Area Networks. IEEE Sensors Journal, 14(3), 797-807. doi:10.1109/jsen.2013.2287895Yao-Chung Chang, Zhi-Sheng Lin, & Jiann-Liang Chen. (2006). Cluster based self-organization management protocols for wireless sensor networks. IEEE Transactions on Consumer Electronics, 52(1), 75-80. doi:10.1109/tce.2006.1605028Fazio, P., De Rango, F., Sottile, C., & Santamaria, A. F. (2013). Routing Optimization in Vehicular Networks: A New Approach Based on Multiobjective Metrics and Minimum Spanning Tree. International Journal of Distributed Sensor Networks, 9(11), 598675. doi:10.1155/2013/598675Saravanan, M., & Madheswaran, M. (2014). A Hybrid Optimized Weighted Minimum Spanning Tree for the Shortest Intrapath Selection in Wireless Sensor Network. Mathematical Problems in Engineering, 2014, 1-8. doi:10.1155/2014/71342

Draft Genome Report of Bacillus altitudinis SORB11, Isolated from the Indian Sector of the Southern Ocean

Here, we present the draft genome sequence of Bacillus altitudinis SORB11, which is tolerant to UV radiation. The strain was isolated from the Indian sector of the Southern Ocean at a depth of 3.8 km. The genome sequence information reported here for B. altitudinis SORB11 gives the basis of its UV resistance mechanism and provides data for further comparative studies with other bacteria resistant to UV radiation

De Novo Assembly of Chickpea Transcriptome Using Short Reads for Gene Discovery and Marker Identification

Chickpea ranks third among the food legume crops production in the world. However, the genomic resources available for chickpea are still very limited. In the present study, the transcriptome of chickpea was sequenced with short reads on Illumina Genome Analyzer platform. We have assessed the effect of sequence quality, various assembly parameters and assembly programs on the final assembly output. We assembled ∼107million high-quality trimmed reads using Velvet followed by Oases with optimal parameters into a non-redundant set of 53 409 transcripts (≥100 bp), representing about 28 Mb of unique transcriptome sequence. The average length of transcripts was 523 bp and N50 length of 900 bp with coverage of 25.7 rpkm (reads per kilobase per million). At the protein level, a total of 45 636 (85.5%) chickpea transcripts showed significant similarity with unigenes/predicted proteins from other legumes or sequenced plant genomes. Functional categorization revealed the conservation of genes involved in various biological processes in chickpea. In addition, we identified simple sequence repeat motifs in transcripts. The chickpea transcripts set generated here provides a resource for gene discovery and development of functional molecular markers. In addition, the strategy for de novo assembly of transcriptome data presented here will be helpful in other similar transcriptome studies