Indoor Particulate Matter during HOMEChem: Concentrations, Size Distributions, and Exposures.

It is important to improve our understanding of exposure to particulate matter (PM) in residences because of associated health risks. The HOMEChem campaign was conducted to investigate indoor chemistry in a manufactured test house during prescribed everyday activities, such as cooking, cleaning, and opening doors and windows. This paper focuses on measured size distributions of PM (0.001-20 μm), along with estimated exposures and respiratory-tract deposition. Number concentrations were highest for sub-10 nm particles during cooking using a propane-fueled stovetop. During some cooking activities, calculated PM2.5 mass concentrations (assuming a density of 1 g cm-3) exceeded 250 μg m-3, and exposure during the postcooking decay phase exceeded that of the cooking period itself. The modeled PM respiratory deposition for an adult residing in the test house kitchen for 12 h varied from 7 μg on a day with no indoor activities to 68 μg during a simulated day (including breakfast, lunch, and dinner preparation interspersed by cleaning activities) and rose to 149 μg during a simulated Thanksgiving day

Multiple-criteria stem bucking (Picea abies L. Karst.) for maximizing monetary value of timber trade

In this study, timber trade scenarios are considered in a wood procurement region of Finland. This multiple objective decision-making situation includes the timber purchase from forest owners and the lumber sales from sawmill to abroad. The situation is further complicated by a number of stem bucking instructions of sawmill during different periods. In practice, this decision problem has been solved by applying single-objective stem bucking instructions in harvesters. Due to the complex nature of the problem, single-objective solution can’t be directly used to support the timber trade in a manner that it is techno-economically relevant to the forest owners and industries. In this study, stand parameters and timber trade attributes were measured in local wood procurement conditions to improving the bucking instructions. Three scenarios of how the simulation system works based on the real stem diameters and optional monetary value of logs are investigated in the timber-trade process. The Finnish timber trade market is subjected to agreements regarding stem bucking regulations. These agreements could be made on the basis of the three criteria suggested in this study accounting for the effects of stand classification on the timber sales of forest owners and the lumber sales of export companies

Novel ZNF414 activity characterized by integrative analysis of ChIP-exo, ATAC-seq and RNA-seq data

Transcription factor binding to DNA is a central mechanism regulating gene expression. Thus, thorough characterization of this process is essential for understanding cellular biology in both health and disease. We combined data from three sequencing-based methods to unravel the DNA binding function of the novel ZNF414 protein in cells representing two tumor types. ChIP-exo served to map protein binding sites, ATAC-seq allowed identification of open chromatin, and RNA-seq examined the transcriptome. We show that ZNF414 is a DNAbinding protein that both induces and represses gene expression. This transcriptional response has an impact on cellular processes related to proliferation and other malignancy-associated functions, such as cell migration and DNA repair. Approximately 20% of the differentially expressed genes harbored ZNF414 binding sites in their promoters in accessible chromatin, likely representing direct targets of ZNF414. De novo motif discovery revealed several putative ZNF414 binding sequences, one of which was validated using EMSA. In conclusion, this study illustrates a highly efficient integrative approach for the characterization of the DNA binding and transcriptional activity of transcription factors.Peer reviewe

Preoperative paroxysmal atrial fibrillation predicts high cardiovascular mortality in patients undergoing surgical aortic valve replacement with a bioprosthesis: CAREAVR study

Background Preoperative permanent atrial fibrillation (AF) is associated with impaired outcome after surgical aortic valve replacement (SAVR). The impact of preoperative paroxysmal AF, however, has remained elusive. Purpose We assessed the impact of preoperative paroxysmal AF on outcome in patients undergoing SAVR with bioprosthesis. Methods A total of 666 patients undergoing isolated AVR with a bioprosthesis were included. Survival data was obtained from the national registry Statistics Finland. Patients were divided into three groups according to the preoperative rhythm: sinus rhythm (n = 502), paroxysmal AF (n = 90), and permanent AF (n = 74). Results Patients in the sinus rhythm and paroxysmal AF groups did not differ with respect to age (P = .484), gender (P = .402) or CHA(2)DS(2)-VASc score (P = .333). At 12-month follow-up, AF was present in 6.2% of sinus rhythm patients and in 42.4% of paroxysmal AF patients (P <.001). During follow-up, incidence of fatal strokes in the paroxysmal AF group was higher compared to sinus rhythm group (1.9 vs 0.4 per 100 patient-years, HR 4.4 95% Cl 1.8-11.0, P = .001). Cardiovascular mortality was higher in the paroxysmal AF group than in the sinus rhythm group (5.0 vs 3.0 per 100 patient-years, HR 1.70 95% CI 1.05-2.76, P = .03) and equal to patients in the permanent AF (5.0 per 100 patient-years). Conclusion Patients undergoing SAVR with bioprosthesis and history of paroxysmal AF had higher risk of developing permanent AF, cardiovascular mortality and incidence of fatal strokes compared to patients with preoperative sinus rhythm. Life-long anticoagulation should be considered in patients with a history of preoperative paroxysmal AF.Peer reviewe

Adverse events and survival with postpericardiotomy syndrome after surgical aortic valve replacement

Peer reviewe

The influence of accretion geometry on the spectral evolution during thermonuclear (type-I) X-ray bursts

Neutron star (NS) masses and radii can be estimated from observations of photospheric radius-expansion X-ray bursts, provided the chemical composition of the photosphere, the spectral colour-correction factors in the observed luminosity range, and the emission area during the bursts are known. By analysing 246 X-ray bursts observed by the Rossi X-ray Timing Explorer from 11 low-mass X-ray binaries, we find a dependence between the persistent spectral properties and the time evolution of the black body normalisation during the bursts. All NS atmosphere models predict that the colour-correction factor decreases in the early cooling phase when the luminosity first drops below the limiting Eddington value, leading to a characteristic pattern of variability in the measured blackbody normalisation. However, the model predictions agree with the observations for most bursts occurring in hard, low-luminosity, &#39;island&#39; spectral states, but rarely during soft, high-luminosity, &#39;banana&#39; states. The observed behaviour may be attributed to the accretion flow, which influences cooling of the NS preferentially during the soft state bursts. This result implies that only the bursts occurring in the hard, low-luminosity spectral states can be reliably used for NS mass and radius determination.</p

Sedimentary molybdenum and uranium : Improving proxies for deoxygenation in coastal depositional environments

Sedimentary molybdenum (Mo) and uranium (U) enrichments are widely used to reconstruct changes in bottom water oxygen conditions in aquatic environments. Until now, most studies using Mo and U have focused on restricted suboxic-euxinic basins and continental margin oxygen minimum zones (OMZs), leaving mildly reducing and oxic (but eutrophic) coastal depositional environments vastly understudied. Currently, it is un-known: (1) to what extent Mo and U enrichment factors (Mo-and U-EFs) can accurately reconstruct oxygen conditions in coastal sites experiencing mild deoxygenation, and (2) to what degree secondary (depositional environmental) factors impact Mo-and U-EFs. Here we investigate 18 coastal sites with varying bottom water redox conditions, which we define by means of five "redox bins", ranging from persistently oxic to persistently euxinic, from a variety of depositional environments. Our results demonstrate that Mo-and U-EF-based redox proxies and sedimentary Mo and U contents can be used to differentiate bottom water oxygen concentration among a range of modern coastal depositional environments. This is underpinned by the contrasting EFs of Mo and U along the redox gradient, which shows a substantial difference of Mo-EFs between redox bins 3-5 (ir/ regularly suboxic - ir/regularly dysoxic - persistently oxic) and of U-EFs between redox bins 1-2 (persistently euxinic - ir/regularly euxinic). Surprisingly, we observe comparatively low redox proxy potential for U in en-vironments of mild deoxygenation (redox bins 3-5). Further, we found that secondary factors can bias Mo-and U-EFs to such an extent that EFs do not reliably reflect bottom water redox conditions. We investigate the impact of limited Mo sedimentary sequestration in sulfidic depositional environments (i.e., the "basin reservoir effect", equilibrium with FeMoS4), Fe/Mn-(oxy)(hydr)oxide "shuttling", oxidative dissolution, the sulfate methane transition zone in the sediment, sedimentation rate, and the local Al background on Mo-and U-EFs.Peer reviewe

Late incidence and recurrence of new-onset atrial fibrillation after isolated surgical aortic valve replacement

Publisher Copyright: © 2021 The AuthorsObjectives: Atrial fibrillation (AF) is a common complication after cardiac surgery. More knowledge is needed about long-term AF recurrence and adverse outcomes related to new-onset AF (NOAF) during the index hospitalization. Methods: A total of 1073 patients underwent isolated surgical aortic valve replacement at the 4 participating hospitals (2002-2014). After the exclusion of patients with a history of any preoperative AF, the final study population included 529 patients in the bioprosthetic and 253 patients in the mechanical valve prosthesis cohort. Median follow-up time was 5.4 (interquartile range, 3.4-8.2) years in the combined cohort. Results: Altogether 333 (42.6%) patients had in-hospital NOAF and 250 (32.0%) AF after hospital discharge. In the mechanical cohort, 64 (25.3%) experienced in-hospital NOAF and 74 (29.2%) AF after hospital discharge, whereas in the bioprosthetic cohort, 269 (50.9%) patients had in-hospital NOAF and 176 (33.3%) AF after hospital discharge. Patients with NOAF during the index hospital stay had a multifold risk of AF after hospital discharge in the combined cohort (hazard ratio [HR], 3.68; 95% confidence interval [CI], 2.82-4.81; P <.0001) as well as in both cohorts separately (bioprosthetic: HR, 4.35; 95% CI, 3.05-6.22; P <.001; mechanical: HR, 2.54; 95% CI, 1.59-4.03; P <.001). Patients with an in-hospital NOAF also had a significantly higher adjusted risk of death during the follow-up in the mechanical (HR, 2.05; 95% CI, 1.10-3.82; P =.025) and bioprosthetic (HR, 1.63; 95% CI, 1.17-2.28; P =.004) valve prosthesis cohorts. Conclusions: NOAF during the index hospitalization is associated with a 2- to 4-fold risk of later AF and 1.6- to 2.0-fold risk of all-cause mortality after mechanical and bioprosthetic surgical aortic valve replacement.Peer reviewe