Transcriptional networks specifying homeostatic and inflammatory programs of gene expression in human aortic endothelial cells.

Endothelial cells (ECs) are critical determinants of vascular homeostasis and inflammation, but transcriptional mechanisms specifying their identities and functional states remain poorly understood. Here, we report a genome-wide assessment of regulatory landscapes of primary human aortic endothelial cells (HAECs) under basal and activated conditions, enabling inference of transcription factor networks that direct homeostatic and pro-inflammatory programs. We demonstrate that 43% of detected enhancers are EC-specific and contain SNPs associated to cardiovascular disease and hypertension. We provide evidence that AP1, ETS, and GATA transcription factors play key roles in HAEC transcription by co-binding enhancers associated with EC-specific genes. We further demonstrate that exposure of HAECs to oxidized phospholipids or pro-inflammatory cytokines results in signal-specific alterations in enhancer landscapes and associate with coordinated binding of CEBPD, IRF1, and NFκB. Collectively, these findings identify cis-regulatory elements and corresponding trans-acting factors that contribute to EC identity and their specific responses to pro-inflammatory stimuli

Right hemithoracic pseudocyst with splenic artery aneurysm: two rare complications of an uncommon disease

Pleural involvement is an uncommon but well recognized complication of chronic pancreatitis, mainly in the form of pleural effusion affecting the left hemithorax. Pancreatic pseudocyst extending to the posterior mediastinum with or without communication with the pleural space is another rare form of this involvement.The treatment of chronic pancreatic pleural effusions and pancreatic pseudocysts generally starts with a conservative approach including bowel rest, drainage of the pleural effusion by repeated thoracentesis or a chest tube, and total parenteral nutrition (TPN) for a period of time determined by the clinical course. Other treatment modalities including percutaneous drainage, endoscopic retrograde cholangiopancreatogram (ERCP) with stenting of the pancreatic duct and surgical drainage are used if conservative approaches fail.We report a patient with a complicated pancreatic pseudocyst who showed an involvement of the posterior mediastinum and right pleural space, with conspectus sparing of the left hemithorax. The patient had a prolonged and complicated course that included recurrence of the pseudocyst with oral feedings and the development of a splenic artery aneurysm. Some of the above findings have been reported separately as uncommon complications of chronic pancreatitis and pancreatic pseudcyst, but to our knowledge a single case with all these complications was not published in the English literature

PENINGKATAN HASIL BELAJAR KIMIA MELALUI PENERAPAN MODEL PEMBELAJARAN EXPERIENTAL LEARNING SISWA KELAS X IPA 2 SMA NEGERI 1 INDRALAYA SELATAN

Penelitian tindakan kelas dilakukan untuk meningkatkan hasil belajar kimia siswa. Subyek penelitian ini adalah siswa kelas X IPA 2 SMA Negeri 1 Indralaya Selatan yang berjumlah 39 siswa melalui penerapan model pembelajaran Experiential Learning. Penelitian dilakukan tiga siklus, setiap siklus dilakukan 2 kali pertemuan. Rata-rata hasil belajar siswa sebelum tindakan (To) sebesar 59,20 dengan ketuntasan belajar sebesar 20,51% (8 siswa). Hasil penelitian menunjukkan skor rata-rata hasil belajar kimia siswa pada siklus I (T1) sebesar 68,28 dengan ketuntasan belajar sebesar 44,44% (16 siswa) dikarenakan penerapan model pembelajaran Experiential Learning. Pada siklus II (T2) skor rata-rata hasil belajar kimia siswa meningkat menjadi 74,33 dengan ketuntasan belajar sebesar 61,54% (24 siswa) dikarenakan siswa memberikan contoh kejadian sehari-hari yang berhubungan dengan materi. Siswa ditegur dan dibimbing dalam melakukan percobaan, observasi, diskusi, dan menjawab soal. Pada siklus III (T3) skor rata-rata hasil belajar kimia peserta didik meningkat menjadi 83,70 dengan ketuntasan belajar sebesar 89,19% (33 siswa) dikarenakan siswa mencari dan menuliskan informasi dari buku dan internet sebagai tambahan belajar sebelum menghubungkan materi pelajaran dengan kehidupan. Siswa yang melakukan percobaan, observasi, diskusi, dan menjawab soal diberikan skor tambah.  Keaktifan siswa juga mengalami peningkatan siklus I rata-rata keaktifan siswa sebesar 54,10% meningkat menjadi 63,67% pada siklus II dan pada siklus III rata-rata keaktifan siswa meningkat menjadi 79,18 %. Hal ini menunjukkan adanya peningkatan hasil belajar dan keaktifan siswa pada mata pelajaran kimia materi stoikiometri kelas X IPA 2 SMA Negeri 1 Indralaya selatan dengan menggunakan model pembelajaran Experiential Learning. Disarankan untuk menggunkan model pembelajaran Experiential Learning ini agar hasil belajar siswa meningkat

PENGEMBANGAN PERLUASAN PRODUK PENERAPAN MODEL PEMBELAJARAN GAVALA BAGI GURU KIMIA DI KABUPATEN OGAN KOMERING ULU SUMATERA SELATAN

Abstrak: Tujuan kegiatan pengabdian ini adalah tersusunnya contoh Rencana Program Pembelajaran kimia SMA dan/atau SMK bernuansa Kurikulum 2013 terintegrasi Model Pembelajaran GAVALA dan simulasi terbatas keterampilan penerapan Model GAVALA. Khalayak sasaran yang sangat strategis untuk kegiatan pengabdian kepada masyarakat ini adalah 10 guru kimia dari SMAN dan SMKN di Kabupaten Ogan Komering Ulu Sumatera Selatan. Model kegiatan adalah pengembang perluasan penerapan produk, metode pelaksanaan berupa presentasi, ipteks yang diintroduksi adalah Model Pembelajaran GAVALA. Peningkatan itu berupa tersusunnya contoh Rencana Program Pembelajaran Model GAVALA dan simulasi terbatas praktik Model Pembelajaran GAVALA di depan para peserta pengabdian. Simpulan dari kegiatan pengabdian pada masyarakat ini adalah Para guru sebagai khalayak sasaran dapat menerapkan Model Pembelajaran GAVALA. Peningkatan kemampuan menerapkan itu berupa tersusunnya contoh kegiatan dalam Rencana Pelaksanaan Pembelajaran bernuansa  Model Pembelajaran GAVALA. Peningkatan itu dibuktikan dengan gain skor sebesar 1,00 dengan kategori tinggi. Saran dari kegiatan pengabdian pada masyarakat ini adalah agar guru khalayak sasaran dapat menerapkan Model Pembelajaran GAVALA pelaksanaan pembelajaran di kelas.       Abstract: The purpose of this service activity is to compile examples of the 2013 High School Chemistry and Vocational High School Chemistry Learning Program Plan integrated with the GAVALA Learning Model and limited simulation of the application skills of the GAVALA Model. A very strategic target audience for community service activities are 10 chemistry teachers from “SMAN” and “SMKN” in Ogan Komering Ulu Regency, South Sumatra. The activity model is the assistance/product expansion, the implementation method in the form of a presentation, the science and technology introduced is the GAVALA Learning Model. The increase was in the form of the preparation of examples of the GAVALA Model Learning Program Plan and the limited simulation of the GAVALA Learning Model Practice in front of the devoted participants. The conclusions of this community service activity are Teachers as the target audience can apply the GAVALA Learning Model. Improving the ability to apply is in the form of compiling examples of core activities in the Learning Implementation Plan nuanced GAVALA Learning Model. The increase was evidenced by a score gain of 1.00 with a high category. The suggestion from this community service activity is that the target audience teachers can apply the GAVALA Learning Model for the implementation of learning in the classroom

Transcriptional networks specifying homeostatic and inflammatory programs of gene expression in human aortic endothelial cells.

Endothelial cells (ECs) are critical determinants of vascular homeostasis and inflammation, but transcriptional mechanisms specifying their identities and functional states remain poorly understood. Here, we report a genome-wide assessment of regulatory landscapes of primary human aortic endothelial cells (HAECs) under basal and activated conditions, enabling inference of transcription factor networks that direct homeostatic and pro-inflammatory programs. We demonstrate that 43% of detected enhancers are EC-specific and contain SNPs associated to cardiovascular disease and hypertension. We provide evidence that AP1, ETS, and GATA transcription factors play key roles in HAEC transcription by co-binding enhancers associated with EC-specific genes. We further demonstrate that exposure of HAECs to oxidized phospholipids or pro-inflammatory cytokines results in signal-specific alterations in enhancer landscapes and associate with coordinated binding of CEBPD, IRF1, and NFκB. Collectively, these findings identify cis-regulatory elements and corresponding trans-acting factors that contribute to EC identity and their specific responses to pro-inflammatory stimuli