15 research outputs found
SME’s perspective on psychosocial risks: From identifying to intervening in four countries
Introduction: Certain aspects of the work design or the organization as well as the social context of work may lead to negative physical, psychological and social outcomes. These aspects are considered psychosocial risks. Portugal, Spain, Italy, and Greece are facing the biggest consequences of the economic crisis with psychosocial risks being exacerbated. In these countries, small and medium enterprises play an important role in the economy. This paper presents the quantitative data from the project, collected in those four countries. The main goal of the survey was to determine the SMEs’ perspective about emergent psychosocial risks, difficulties and concerns related to its prevention as well as to assess their knowledge and existing practices regarding psychosocial risks prevention.info:eu-repo/semantics/publishedVersio
Participative prevention of psychosocial emergent risks in small and medium enterprises Overview of a collaborative project
info:eu-repo/semantics/publishedVersio
Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration
Cells sense gradients of extracellular cues and generate polarized responses such as cell migration and neurite initiation. There is static information on the intracellular signaling molecules involved in these responses, but how they dynamically orchestrate polarized cell behaviors is not well understood. A limitation has been the lack of methods to exert spatial and temporal control over specific signaling molecules inside a living cell. Here we introduce optogenetic tools that act downstream of native G protein–coupled receptor (GPCRs) and provide direct control over the activity of endogenous heterotrimeric G protein subunits. Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling. In immune cells exposed to spatially uniform chemoattractants, these optogenetic tools allow us to create reversible gradients of signaling activity. Migratory responses generated by this approach show that a gradient of active G protein αi and βγ subunits is sufficient to generate directed cell migration. They also provide the most direct evidence so for a global inhibition pathway triggered by Gi signaling in directional sensing and adaptation. These optogenetic tools can be applied to interrogate the mechanistic basis of other GPCR-modulated cellular functions
Expression and Membrane Topology of Anopheles gambiae Odorant Receptors in Lepidopteran Insect Cells
A lepidopteran insect cell-based expression system has been employed to express three Anopheles gambiae odorant receptors (ORs), OR1 and OR2, which respond to components of human sweat, and OR7, the ortholog of Drosophila's OR83b, the heteromerization partner of all functional ORs in that system. With the aid of epitope tagging and specific antibodies, efficient expression of all ORs was demonstrated and intrinsic properties of the proteins were revealed. Moreover, analysis of the orientation of OR1 and OR2 on the cellular plasma membrane through the use of a novel ‘topology screen’ assay and FACS analysis demonstrates that, as was recently reported for the ORs in Drosophila melanogaster, mosquito ORs also have a topology different than their mammalian counterparts with their N-terminal ends located in the cytoplasm and their C-terminal ends facing outside the cell. These results set the stage for the production of mosquito ORs in quantities that should permit their detailed biochemical and structural characterization and the exploration of their functional properties