Dirac versus Reduced Quantization of the Poincar\'{e} Symmetry in Scalar Electrodynamics

The generators of the Poincar\'{e} symmetry of scalar electrodynamics are quantized in the functional Schr\"{o}dinger representation. We show that the factor ordering which corresponds to (minimal) Dirac quantization preserves the Poincar\'{e} algebra, but (minimal) reduced quantization does not. In the latter, there is a van Hove anomaly in the boost-boost commutator, which we evaluate explicitly to lowest order in a heat kernel expansion using zeta function regularization. We illuminate the crucial role played by the gauge orbit volume element in the analysis. Our results demonstrate that preservation of extra symmetries at the quantum level is sometimes a useful criterion to select between inequivalent, but nevertheless self-consistent, quantization schemes.Comment: 24 page

Free-Flight Terrestrial Rocket Lander Demonstration for NASA's Autonomous Landing and Hazard Avoidance Technology (ALHAT) System

The Autonomous Landing Hazard Avoidance Technology (ALHAT) Project is chartered to develop and mature to a Technology Readiness Level (TRL) of six an autonomous system combining guidance, navigation and control with terrain sensing and recognition functions for crewed, cargo, and robotic planetary landing vehicles. The ALHAT System must be capable of identifying and avoiding surface hazards to enable a safe and accurate landing to within tens of meters of designated and certified landing sites anywhere on a planetary surface under any lighting conditions. Since its inception in 2006, the ALHAT Project has executed four field test campaigns to characterize and mature sensors and algorithms that support real-time hazard detection and global/local precision navigation for planetary landings. The driving objective for Government Fiscal Year 2012 (GFY2012) is to successfully demonstrate autonomous, real-time, closed loop operation of the ALHAT system in a realistic free flight scenario on Earth using the Morpheus lander developed at the Johnson Space Center (JSC). This goal represents an aggressive target consistent with a lean engineering culture of rapid prototyping and development. This culture is characterized by prioritizing early implementation to gain practical lessons learned and then building on this knowledge with subsequent prototyping design cycles of increasing complexity culminating in the implementation of the baseline design. This paper provides an overview of the ALHAT/Morpheus flight demonstration activities in GFY2012, including accomplishments, current status, results, and lessons learned. The ALHAT/Morpheus effort is also described in the context of a technology path in support of future crewed and robotic planetary exploration missions based upon the core sensing functions of the ALHAT system: Terrain Relative Navigation (TRN), Hazard Detection and Avoidance (HDA), and Hazard Relative Navigation (HRN)

The global distribution of Bacillus anthracis and associated anthrax risk to humans, livestock and wildlife.

Bacillus anthracis is a spore-forming, Gram-positive bacterium responsible for anthrax, an acute infection that most significantly affects grazing livestock and wild ungulates, but also poses a threat to human health. The geographic extent of B. anthracis is poorly understood, despite multi-decade research on anthrax epizootic and epidemic dynamics; many countries have limited or inadequate surveillance systems, even within known endemic regions. Here, we compile a global occurrence dataset of human, livestock and wildlife anthrax outbreaks. With these records, we use boosted regression trees to produce a map of the global distribution of B. anthracis as a proxy for anthrax risk. We estimate that 1.83 billion people (95% credible interval (CI): 0.59-4.16 billion) live within regions of anthrax risk, but most of that population faces little occupational exposure. More informatively, a global total of 63.8 million poor livestock keepers (95% CI: 17.5-168.6 million) and 1.1 billion livestock (95% CI: 0.4-2.3 billion) live within vulnerable regions. Human and livestock vulnerability are both concentrated in rural rainfed systems throughout arid and temperate land across Eurasia, Africa and North America. We conclude by mapping where anthrax risk could disrupt sensitive conservation efforts for wild ungulates that coincide with anthrax-prone landscapes

Multinuclear Solid-State NMR Study of Local Structure and Dynamics in Li0.7Nb3S4

The present work focuses on channel-structured Li0.7Nb3S4, where, among others, the question of the dimensionality of the diffusion pathway arises

A computerized intervention to promote colorectal cancer screening for underserved populations: Theoretical background and algorithm development

Objective The aim of this exploratory study was to assess factors deemed by patients as “important” as they planned and considered undergoing colorectal cancer (CRC) screening, and to use this data to design a computer-delivered intervention to promote screening. Methods Fifty participants 50 years or older, not up-to-date with current recommended CRC screening guidelines, were recruited from a primary care clinic. A semi-structured interview focused on aspects of preparing for colorectal cancer screening was administered; after transcription, researchers used triangulation and consensus to identify relevant themes and concepts. Results Four main themes were identified that dealt with issues important for both FOBT and colonoscopy planning: personal concerns, reminders, communication with healthcare providers and obtaining test results. FOBT specific themes included: sample collection and return. For colonoscopy screening, themes included: scheduling, intervention questions, colonoscopy preparation, and transportation. These can be classified as barrier, process and accessory themes. The developed computer-administered implementation intentions algorithm addressed all the identified concerns in a planned and sequential manner, in order to facilitate planning for CRC screening. Conclusions The results of this study suggest that appropriate reminders, explanations of procedures, and patient understanding of temporary life disruptions, help patients develop and accept a detailed screening plan

Predictive markers and risk factors in canine pyometra

Pyometra is a common and life-threatening disease in intact female dogs, which is generally treated by surgery. Early identification of dogs with high risk of complications or poor prognosis is valuable for optimising treatment and increase survival. The objectives of this thesis were to detect predictive markers for prognosis and outcome of pyometra by investigating clinical and pathophysiological responses and to explore the breed-dependent risk for pyometra and mammary tumours (MTs). Leucopaenia was the most important predictive variable, associated with an 18-fold increased risk for peritonitis (present in 13% of the dogs) and an over 3.5-fold increased risk for prolonged postoperative hospitalisation. Fever or hypothermia was linked with an increased risk for peritonitis and dogs with moderate to severely depressed general condition or pale mucous membranes had an increased risk for prolonged postoperative hospitalisation. These results show that commonly explored clinical variables may be helpful for predicting prognosis. Blood concentrations of the acute phase proteins, C-reactive protein and serum amyloid A (SAA) were found to be increased in dogs with pyometra, whereas concentrations of albumin, insulin-like growth factor-I, and iron were decreased. Importantly, SAA concentrations were higher in the dogs that also suffered from sepsis. Though unspecific, SAA could therefore be a potential marker for identifying more severely affected dogs. The neuroendocrine protein chromogranin A was measured by its breakdown products catestatin and vasostatin. Catestatin concentrations were decreased in pyometra whereas vasostatin concentrations did not differ compared to healthy dogs. None of these investigated inflammatory mediators or chromogranin A were useful for outcome prediction as measured by postoperative hospitalisation. The incidence of pyometra in 110 different breeds was studied using insurance data. Before 10 years of age, 19% of all female dogs had suffered from the disease. Breed greatly affected the risk of both pyometra and MTs. In summary, these findings show that clinical and laboratory data and analysis of inflammatory variables can be helpful for predicting prognosis and assessing severity in dogs with pyometra. Breed considerably affects the risk of pyometra and MTs, and the information presented in this thesis will be valuable for evaluating possible health benefits of spaying in individual dogs, based on the risk of developing these diseases

Hairy Canola (Brasssica napus) re-visited: Down-regulating TTG1 in an AtGL3-enhanced hairy leaf background improves growth, leaf trichome coverage, and metabolite gene expression diversity

Primer sequences used in the construction and analysis of B. napus transgenic lines. Table S1B. Blast of batch leaf Q-PCR primers to the B. rapa, B. oleracea, and B. napus genomes for five trichome regulatory genes and two control genes in B. napus. Table S1C. “Detectable” B. napus homologues of five trichome regulatory genes in first true leaves (from RNA sequencing). Table S1D. BlastP for five Arabidopsis trichome regulatory genes against the Brassica napus genome in NCBI. Table S2A. Differentially expressed leaf trichome ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy line K-5-8 relative to semi-glabrous cv. Westar. Table S2B. Leaf trichome genes with no significant expression differences (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy line K-5-8 relative to semi-glabrous cv. Westar. Table S3. Differentially expressed leaf flavonoid ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S4. Differentially expressed leaf phenylpropanoid and lignin ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S5. Differentially expressed leaf phenolic ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S6. Differentially expressed leaf shikimate ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S7. Differentially expressed leaf isoprenoid and terpene ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S8. Differentially expressed leaf glucosinolate-related and miscellaneous sulphur-related ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S9. Differentially expressed leaf alkaloid-related and miscellaneous N-metabolizing ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S10. Differentially expressed leaf cell wall structural carbohydrate ESTs ((p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S11. Differentially expressed leaf mucilage ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S12. Differentially expressed leaf wax ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S13. Differentially expressed leaf hormone ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S14. Differentially expressed leaf secondary metabolism ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S15. Differentially expressed leaf redox-related ESTs (p < 0.05)) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S16. Differentially expressed leaf protein modification ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S17. Differentially expressed leaf protein degradation ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. Table S18. Differentially expressed leaf transcription factor ESTs (p < 0.05) in hairy AtGL3+ B. napus or ultra-hairy K-5-8 relative to semi-glabrous cv. Westar. (XLSX 400 kb

Auction-based approach to resolve the scheduling problem in the steel making process

Steel production is an extremely complex process and determining coherent schedules for the wide variety of production steps in a dynamic environment, where disturbances frequently occur, is a challenging task. In the steel production process, the blast furnace continuously produces liquid iron, which is transformed into liquid steel in the melt shop. The majority of the molten steel passes through a continuous caster to form large steel slabs, which are rolled into coils in the hot strip mill. The scheduling system of these processes has very different objectives and constraints, and operates in an environment where there is a substantial quantity of real-time information concerning production failures and customer requests. The steel making process, which includes steel making followed by continuous casting, is generally the main bottleneck in steel production. Therefore, comprehensive scheduling of this process is critical to improve the quality and productivity of the entire production system. This paper addresses the scheduling problem in the steel making process. The methodology of winner determination using the combinatorial auction process is employed to solve the aforementioned problem. In the combinatorial auction, allowing bidding on a combination of assets offers a way of enhancing the efficiency of allocating the assets. In this paper, the scheduling problem in steel making has been formulated as a linear integer program to determine the scheduling sequence for different charges. Bids are then obtained for sequencing the charges. Next, a heuristic approach is used to evaluate the bids. The computational results show that our algorithm can obtain optimal or near-optimal solutions for combinatorial problems in a reasonable computation time. The proposed algorithm has been verified by a case study

Hairy Canola (Brasssica napus) re-visited: Down-regulating TTG1 in an AtGL3-enhanced hairy leaf background improves growth, leaf trichome coverage, and metabolite gene expression diversity

Background Through evolution, some plants have developed natural resistance to insects by having hairs (trichomes) on leaves and other tissues. The hairy trait has been neglected in Brassica breeding programs, which mainly focus on disease resistance, yield, and overall crop productivity. In Arabidopsis, a network of three classes of proteins consisting of TTG1 (a WD40 repeat protein), GL3 (a bHLH factor) and GL1 (a MYB transcription factor), activates trichome initiation and patterning. Introduction of a trichome regulatory gene AtGL3 from Arabidopsis into semi-glabrous Brassica napus resulted in hairy canola plants which showed tolerance to flea beetles and diamondback moths; however plant growth was negatively affected. In addition, the role of BnTTG1 transcription in the new germplasm was not understood. Results Here, we show that two ultra-hairy lines (K-5-8 and K-6-3) with BnTTG1 knock-down in the hairy AtGL3+ B. napus background showed stable enhancement of trichome coverage, density, and length and restored wild type growth similar to growth of the semi-glabrous Westar plant. In contrast, over-expression of BnTTG1 in the hairy AtGL3+ B. napus background gave consistently glabrous plants of very low fertility and poor stability, with only one glabrous plant (O-3-7) surviving to the T3 generation. Q-PCR trichome gene expression data in leaf samples combining several leaf stages for these lines suggested that BnGL2 controlled B. napus trichome length and out-growth and that strong BnTTG1 transcription together with strong GL3 expression inhibited this process. Weak expression of BnTRY in both glabrous and trichome-bearing leaves of B. napus in the latter Q-PCR experiment suggested that TRY may have functions other than as an inhibitor of trichome initiation in the Brassicas. A role for BnTTG1 in the lateral inhibition of trichome formation in neighbouring cells was also proposed for B. napus. RNA sequencing of first leaves identified a much larger array of genes with altered expression patterns in the K-5-8 line compared to the hairy AtGL3+ B. napus background (relative to the Westar control plant). These genes particularly included transcription factors, protein degradation and modification genes, but also included pathways that coded for anthocyanins, flavonols, terpenes, glucosinolates, alkaloids, shikimates, cell wall biosynthesis, and hormones. A 2nd Q-PCR experiment was conducted on redox, cell wall carbohydrate, lignin, and trichome genes using young first leaves, including T4 O-3-7-5 plants that had partially reverted to yield two linked growth and trichome phenotypes. Most of the trichome genes tested showed to be consistant with leaf trichome phenotypes and with RNA sequencing data in three of the lines. Two redox genes showed highest overall expression in K-5-8 leaves and lowest in O-3-7-5 leaves, while one redox gene and three cell wall genes were consistently higher in the two less robust lines compared with the two robust lines. Conclusion The data support the strong impact of BnTTG1 knockdown (in the presence of strong AtGL3 expression) at restoring growth, enhancing trichome coverage and length, and enhancing expression and diversity of growth, metabolic, and anti-oxidant genes important for stress tolerance and plant health in B. napus. Our data also suggests that the combination of strong (up-regulated) BnTTG1 expression in concert with strong AtGL3 expression is unstable and lethal to the plant