Human Skin Temperature and Biological Clocks: A Laboratory Exercise for Physiology Students

A simple laboratory experiment is described, consisting of taking the mean temperature between two fingers of human subjects. The results from comparing male and female students is presented. The change in skin temperature over four hours is documented, to demonstrate the importance of doing standardized experiments at the same time of day

How Should We Manage All Those Databases?

In an organization where there are many DBAs working with many instances and databases on many machines with many developers � how do you manage all of this without total chaos? This paper will outline how the central Database Support organization at Lockheed Martin Energy Systems in Oak Ridge, TN manages more than 250 instances on more than 90 systems with a variety of operating systems. This discussion will include how tasks and responsibilities are divided between System DBAs, Application Project DBAs, and developers. The use of standards as well as local routines to maintain the systems will be discussed. Information on the type of communications used to keep the different group informed and up-to-date will also be presented

Complement and cytokine response in acute Thrombotic Thrombocytopenic Purpura

Complement dysregulation is key in the pathogenesis of atypical Haemolytic Uraemic Syndrome (aHUS), but no clear role for complement has been identified in Thrombotic Thrombocytopenic Purpura (TTP). We aimed to assess complement activation and cytokine response in acute antibody-mediated TTP. Complement C3a and C5a and cytokines (interleukin (IL)-2, IL-4, IL-6, IL-10, tumour necrosis factor, interferon-γ and IL-17a) were measured in 20 acute TTP patients and 49 remission cases. Anti-ADAMTS13 immunoglobulin G (IgG) subtypes were measured in acute patients in order to study the association with complement activation. In acute TTP, median C3a and C5a were significantly elevated compared to remission, C3a 63·9 ng/ml vs. 38·2 ng/ml (P < 0·001) and C5a 16·4 ng/ml vs. 9·29 ng/ml (P < 0·001), respectively. Median IL-6 and IL-10 levels were significantly higher in the acute vs. remission groups, IL-6: 8 pg/ml vs. 2 pg/ml (P = 0·003), IL-10: 6 pg/ml vs. 2 pg/ml (P < 0·001). C3a levels correlated with both anti-ADAMTS13 IgG (rs  = 0·604, P = 0·017) and IL-10 (rs  = 0·692, P = 0·006). No anti-ADAMTS13 IgG subtype was associated with higher complement activation, but patients with the highest C3a levels had 3 or 4 IgG subtypes present. These results suggest complement anaphylatoxin levels are higher in acute TTP cases than in remission, and the complement response seen acutely may relate to anti-ADAMTS13 IgG antibody and IL-10 levels

Reconstructing the tenor ‘pharyngeal voice’: a historical and practical investigation

One of the defining moments of operatic history occurred in April 1837 when upon returning to Paris from study in Italy, Gilbert Duprez (1806–1896) performed the first ‘do di petto’, or high c′′ ‘from the chest’, in Rossini’s Guillaume Tell. However, according to the great pedagogue Manuel Garcia (jr.) (1805–1906) tenors like Giovanni Battista Rubini (1794–1854) and Garcia’s own father, tenor Manuel Garcia (sr.) (1775–1832), had been singing the ‘do di petto’ for some time. A great deal of research has already been done to quantify this great ‘moment’, but I wanted to see if it is possible to define the vocal qualities of the tenor voices other than Duprez’, and to see if perhaps there is a general misunderstanding of their vocal qualities. That investigation led me to the ‘pharyngeal voice’ concept, what the Italians call falsettone. I then wondered if I could not only discover the techniques which allowed them to have such wide ranges, fioritura, pianissimi, superb legato, and what seemed like a ‘do di petto’, but also to reconstruct what amounts to a ‘lost technique’. To accomplish this, I bring my lifelong training as a bel canto tenor and eighteen years of experience as a classical singing teacher to bear in a partially autoethnographic study in which I analyse the most important vocal treatises from Pier Francesco Tosi’s (c. 1653–1732) treatise ‘Opinioni de' cantori antichi e moderni‘ (1723) to ‘Garcia’s Treatise on the Art of Singing’ (1924). I analysed the treatises for concepts of registration, timbre, breathing and resonance tuning. Subsequently, I researched contemporary accounts of several tenors to develop a ‘picture’ of their individual voices and to distinguish voice types, and then analysed multiple extracts from operas to determine range, tessitura, dynamic ability, and melodic contour markers for each singer. Using performance practice methodologies in the teaching studio, I was able combine all these elements to produce a valid and effective historically informed reconstruction of the historical tenor ‘pharyngeal voice’ and pedagogy

Unprecedented chemical transformation: crystallographic evidence for 1,1,2,2-tetrahydroxyethane captured within an Fe6Dy3 single molecule magnet

A nonanuclear {Fe6Dy3} coordination cluster displaying SMM behaviour in which an unprecedented chemical transformation provides structural information for the existence of 1,1,2,2-tetrahydroxyethane is reported

Tunable, Concurrent Multiband, Single Chain Radio Architecture for Low Energy 5G-RANs

This invited paper considers a key next step in the design of radio architectures aimed at supporting low energy consumption in 5G heterogeneous radio access networks. State-of-the-art mobile radios usually require one RF transceiver per standard, each working separately at any given time. Software defined radios, while spanning a wide range of standards and frequency bands, also work separately at any specific time. In 5G radio access networks, where continuous, multiband connectivity is envisaged, this conventional radio architecture results in high network power consumption. In this paper, we propose the novel concept of a concurrent multiband frequency-agile radio (CM-FARAD) architecture, which simultaneously supports multiple standards and frequency bands using a single, tunable transceiver. We discuss the subsystem radio design approaches for enabling the CM-FARAD architecture, including antennas, power amplifiers, low noise amplifiers and analogue to digital converters. A working prototype of a dual-band CM-FARAD test-bed is also presented together with measured salient performance characteristics

Comparative genomics in chicken and Pekin duck using FISH mapping and microarray analysis

BACKGROUND: The availability of the complete chicken (Gallus gallus) genome sequence as well as a large number of chicken probes for fluorescent in-situ hybridization (FISH) and microarray resources facilitate comparative genomic studies between chicken and other bird species. In a previous study, we provided a comprehensive cytogenetic map for the turkey (Meleagris gallopavo) and the first analysis of copy number variants (CNVs) in birds. Here, we extend this approach to the Pekin duck (Anas platyrhynchos), an obvious target for comparative genomic studies due to its agricultural importance and resistance to avian flu. RESULTS: We provide a detailed molecular cytogenetic map of the duck genome through FISH assignment of 155 chicken clones. We identified one inter- and six intrachromosomal rearrangements between chicken and duck macrochromosomes and demonstrated conserved synteny among all microchromosomes analysed. Array comparative genomic hybridisation revealed 32 CNVs, of which 5 overlap previously designated "hotspot" regions between chicken and turkey. CONCLUSION: Our results suggest extensive conservation of avian genomes across 90 million years of evolution in both macro- and microchromosomes. The data on CNVs between chicken and duck extends previous analyses in chicken and turkey and supports the hypotheses that avian genomes contain fewer CNVs than mammalian genomes and that genomes of evolutionarily distant species share regions of copy number variation ("CNV hotspots"). Our results will expedite duck genomics, assist marker development and highlight areas of interest for future evolutionary and functional studies

Public health impact of low-dose aspirin on colorectal cancer, cardiovascular disease and safety in the UK – Results from micro-simulation model

Background: Low-dose aspirin therapy reduces the risk of cardiovascular disease and may have a positive effect on the prevention of colorectal cancer. We evaluated the population-level expected effect of regular low-dose aspirin use on cardiovascular disease (CVD), colorectal cancer (CRC), gastrointestinal bleeding, symptomatic peptic ulcers, and intracranial hemorrhage, using a microsimulation study design. Methods: We used individual-level state transition modeling to assess the impact of aspirin in populations aged 50–59 or 60–69 years old indicated for low-dose aspirin usage for primary or secondary CVD prevention. Model parameters were based on data from governmental agencies from the UK or recent publications. Results: In the 50–59 years cohort, a decrease in incidence rates (IRs per 100 000 person years) of non-fatal CVD (-203 and -794) and fatal CVD (-97 and-381) was reported in the primary and secondary CVD prevention setting, respectively. The IR reduction of CRC (-96 and -93) was similar for primary and secondary CVD prevention. The IR increase of non-fatal (116 and 119) and fatal safety events (6 and 6) was similar for primary and secondary CVD prevention. Similar results were obtained for the 60–69 years cohort. Conclusions: The decrease in fatal CVD and CRC events was larger than the increase in fatal safety events and this difference was more pronounced when low-dose aspirin was used for secondary compared to primary CVD prevention. These results provide a comprehensive image of the expected effect of regular low-dose aspirin therapy in a UK population indicated to use aspirin for CVD prevention. © 202