Contribution of Grasses to Soil Fertility and Improved Livelihoods

Vegetable farming is increasing in Nepal as it provides better economic returns than growing other crops, especially in areas that have easy access to markets. Vegetable farming demands intensive care and balanced supplies of nutrients. Therefore, farmers cultivate vegetables near their residence and because vegetable growing is more profitable, farmers allocate more resources, including organic manure, for its cultivation. In general, using more organic manure on vegetables means that less organic manure is available for non-vegetable crops and farms, unless alternative arrangements are made for producing more organic manure or manure of higher quality

Pregnancy Induced Alterations of Reproductive Tract Collagen and Elastin in a Murine Model

This thesis reports on structural and dynamical modifications of reproductive tract elastin and collagen as a function of parity. Pelvic floor dysfunction, including pelvic organ prolapse (POP) is a major concern affecting female health worldwide, leading to surgeries costing billions of dollars annually. Collagen, elastic fibers, and proteoglycans are major extracellular matrix (ECM) components found in connective tissues. Vaginal child birth, advancing age, and disruption or dysfunction of connective tissue are major risk factors of POP. In the female reproductive tract, the assembly of elastic fibers is crucial for the pelvic floor support. Any disturbance in the synthesis, assembly, and degradation of elastic fibers in the reproductive tract during parturition or aging may result in pelvic floor dysfunction. Various experimental methods were implemented to probe the structural and dynamical changes of these proteins, as a function of the number of pregnancies, including 13C nuclear magnetic resonance (NMR), 2H NMR, spectrophotometry, MALDI mass spectrometry, and histology. Results of this study provide the gynecological community with further understanding relating to how elastin and collagen of the reproductive tract are altered as a function of the number of births, and may contribute to the loss of tissue elasticity and the onset of pelvic floor dysfunction. The concentration of elastin in the reproductive tract is approximately 1.5% by weight making quantification challenging. This thesis has provided an important step in understanding how pregnancy and/or parturition may lead to alterations of reproductive tract elastin. In the elastin study, experimental data unveils remarkably different 13C spectra for virgin rat cohorts may be due to higher concentration of MMP-2 and MMP-9, which degrade elastin, compared to elastin from the multiparous rat cohorts. Single pregnancy cohorts exhibit spectra similar to the virgin rat, whereas elastin from cohorts that have undergone three pregnancies resembled that from the multiparous rat cohorts. By applying solid state NMR relaxation measurements the dynamics of major backbone and side chain carbon nuclei in the major amino acids, (e.g., glycine, valine, proline, etc.) were found to vary significantly in virgin cohorts in comparison to multiparous cohorts. Furthermore, the concentration of desmosine was found to be highest in the multiparous cohorts and similar to that found in other mammalian elastins. While the microscopic structure of elastin in multiparous cohorts appears strikingly similar to elastin in other mammalian tissues, the elastic fibers are fragmented and highly tortuous. Our current model suggests that the elastic fiber fragmentation, which occurs over length scales of approximately 10-50 micrometers (the length of an elastic fiber), together with collagen content may play a crucial role in tissue scaffolding than alterations in microscopic structure (e.g. desmosine crosslinks). Collagen, another major component of extracellular matrix protein in the reproductive tract, also undergoes significant remodeling and alterations during pregnancy. Interestingly, the structure of collagen was found to be intact despite tremendous remodeling and alterations during pregnancy. However, collagen content was observed to be highest in the virgin cohorts compared to multiparous cohorts, by histological methods. 2H NMR relaxation methods were used to characterize the dynamics and distribution of water in both collagen and elastin as well as only elastin of various cohorts. The redistribution of water due to pregnancy and/or parturition results in additional water reservoir in postpartum and multiparous cohorts in collagen and elastin samples. However, in only elastin from multiparous and virgin cohorts, dynamics of water were observed to vary significantly across cohorts due to fiber fragmentation, and microscopic alterations driven by MMP concentration. Apart from reproductive tract collagen and elastin, this thesis has provided detailed measurements relating to the effects of ultraviolet (UV) irradiation on elastic fibers. UV-A (365 nm) exposure of elastic fibers was shown to result in distinct cracks and disruption in regular array of elastic fibers. Additionally, the concentration of desmosine in elastin is reduced in comparison to the nonirradiated elastic fibers after UV irradiation. This portion of the thesis has introduced a method for readily measuring the concentration of desmosine in tissues and other bodily fluids by MALDI mass spectrometry

Effect of biscuit baking conditions on the stability of microencapsulated 5-methyltetrahydrofolic acid and their physical properties

Among the folate compounds, 5-methyltetrahydrofolic acid (5-CH3THF) is regarded as one of the most bioactive forms of folate. It is regarded as the better source of folate to humans as compared to folic acid, a synthetic form of folate, which is used for fortifying foods to prevent the incidence of neural tube defects in the new born babies. The use of 5-CH3THF as an alternative fortificant, in place of folic acid, has been explored by various researchers. However, fortification of 5-CH3THF is problematic due to its lower stability. This study investigated the stability of microencapsulated 5-CH3THF in biscuits baked at various temperatures and times as well as changes in their physical properties. Microcapsule with pectin and alginate ratio of 80:20, prepared by spray drying, gave the highest retention (68.6%) of the 5-CH3THF, therefore, chosen for fortification. The encapsulated and unencapsulated 5-CH3THF were mixed separately with flour and biscuit ingredients and baked at 180°C, 200°C and 220°C, each for 5, 9 and 12 min. The inclusion of encapsulated and unencapsulated 5-CH3THF in the biscuit formulation and subsequent baking at various temperatures and times resulted in retention of 5-CH3THF from 19.1% to 1.7%. Microencapsulation of 5-CH3THF slightly improved the retention of 5-CH3THF over unencapsuated biscuits at 180°C for 5 min, but almost no such effect was achieved under baking temperatures of 200°C and 220°C. Physical analysis showed darker colour, harder texture and lower moisture content for biscuits baked at higher test temperatures. It seems intense heating condition that caused “over baking” of the biscuit likely to be responsible for the loss of the vitamin as well as less desirable physical properties of the biscuits

The High-Order-Multiplicity of Unusually Wide M-dwarf Binaries: Eleven New Triple and Quadruple Systems

M-dwarfs in extremely wide binary systems are very rare, and may thus have different formation processes from those found as single stars or close binaries in the field. In this paper we search for close companions to a new sample of 36 extremely wide M-dwarf binaries, covering a spectral type range of M1 to M5 and a separation range of 600 - 6500 AU. We discover 10 new triple systems and one new quadruple system. We carefully account for selection effects including proper motion, magnitude limits, the detection of close binaries in the SDSS, and other sample biases. The bias-corrected total high-order-multiple fraction is 45% (+18%/-16%) and the bias-corrected incidence of quadruple systems is < 5%, both statistically compatible with that found for the more common close M-dwarf multiple systems. Almost all the detected companions have similar masses to their primaries, although two very low mass companions, including a candidate brown dwarf, are found at relatively large separations. We find that the close-binary separation distribution is strongly peaked towards < 30AU separations. There is marginally significant evidence for a change in high-order M-dwarf multiplicity with binding energy and total mass. We also find 2-sigma evidence of an unexpected increased high-order-multiple fraction for the widest targets in our survey, with a high-order-multiple fraction of 21% (+17%/-7%) for systems with separations up to 2000AU, compared to 77% (+9%/-22%) for systems with separations > 4000AU. These results suggest that the very widest M-dwarf binary systems need higher masses to form or to survive.Comment: 11 pages, 14 figures, accepted for publication in Ap

Periodic Accretion From A Circumbinary Disk In The Young Binary UZ Tau E

Close pre-main-sequence binary stars are expected to clear central holes in their protoplanetary disks, but the extent to which material can flow from the circumbinary disk across the gap onto the individual circumstellar disks has been unclear. In binaries with eccentric orbits, periodic perturbation of the outer disk is predicted to induce mass flow across the gap, resulting in accretion that varies with the binary period. This accretion may manifest itself observationally as periodic changes in luminosity. Here we present a search for such periodic accretion in the pre-main-sequence spectroscopic binary UZ Tau E. We present BVRI photometry spanning 3 years; we find that the brightness of UZ Tau E is clearly periodic, with a best-fit period of 19.16 +/- 0.04 days. This is consistent with the spectroscopic binary period of 19.13 days, refined here from analysis of new and existing radial velocity data. The brightness of UZ Tau E shows significant random variability, but the overall periodic pattern is a broad peak in enhanced brightness, spanning more than half the binary orbital period. The variability of the H alpha line is not as clearly periodic, but given the sparseness of the data, some periodic component is not ruled out. The photometric variations are in good agreement with predictions from simulations of binaries with orbital parameters similar to those of UZ Tau E, suggesting that periodic accretion does occur from circumbinary disks, replenishing the inner circumstellar disks and possibly extending the timescale over which they might form planets

Neutron scattering study of magnetic phase separation in nanocrystalline La5/8_{5/8}Ca3/8_{3/8}MnO3_3

We demonstrate that magnetic phase separation and competing spin order in the colossal magnetoresistive (CMR) manganites can be directly explored via tuning strain in bulk samples of nanocrystalline La$_{1-x}$Ca$_x$MnO$_3$. Our results show that strain can be reversibly frozen into the lattice in order to stabilize coexisting antiferromagnetic domains within the nominally ferromagnetic metallic state of La$_{5/8}$Ca$_{3/8}$MnO$_3$. The measurement of tunable phase separation via magnetic neutron powder diffraction presents a direct route of exploring the correlated spin properties of phase separated charge/magnetic order in highly strained CMR materials and opens a potential avenue for realizing intergrain spin tunnel junction networks with enhanced CMR behavior in a chemically homogeneous material.Comment: 6 pages, 4 figures. New figure and text added to manuscrip

Improving post-partum family planning services provided by female community health volunteers in Nepal: a mixed methods study.

BACKGROUND: Family planning services in the post-partum period, termed post-partum family planning (PPFP) is critical to cover the unmet need for contraception, especially when institutional delivery rates have increased. However, the intention to choose PPFP methods such as post-partum intrauterine devices (PPIUD) remains low in countries such as Nepal. Community health workers such as Female Community Health Volunteers (FCHVs) could play an important role in improving the service coverage of PPFP in Nepal. However, their knowledge of PPFP and community-based services related to PPFP remain unclear. This study aims to assess the effect on community-based PPFP services by improving FCHV's knowledge through orientation on PPFP. METHODS: We conducted this mixed-methods study in Morang District in Nepal. The intervention involved orientation of FCHVs on PPFP methods. We collected quantitative data from three sources; via a survey of FCHVs that assessed their knowledge before and after the intervention, from their monthly reporting forms on counseling coverage of women at different stages of pregnancy from the communities, and by interviewing mothers in their immediate post-partum period in two selected hospitals. We also conducted six focus group discussions with the FCHVs to understand their perception of PPFP and the intervention. We performed descriptive and multivariable analyses for quantitative results and thematic analysis for qualitative data. RESULTS: In total, 230 FCHVs participated in the intervention and their knowledge of PPFP improved significantly after it. The intervention was the only factor significantly associated with their improved knowledge (adjusted odds ratio = 24, P < 0.001) in the multivariable analysis. FCHVs were able to counsel 83.3% of 1872 mothers at different stages of pregnancy in the communities. In the two hospitals, the proportion of mothers in their immediate post-partum period whom reported they were counseled by FCHVs during their pregnancy increased. It improved from 7% before the intervention to 18.1% (P < 0.001) after the intervention. The qualitative findings suggested that the intervention improved their knowledge in providing PPFP counseling. CONCLUSION: The orientation improved the FCHV's knowledge of PPFP and their community-based counseling. Follow-up studies are needed to assess the longer term effect of the FCHV's role in improving community-based PPFP services

Zn-induced spin dynamics in overdoped La2−x_{2-x}Srx_xCu1−y_{1-y}Zny_yO4_4

Spin fluctuations and the local spin susceptibility in isovalently Zn-substituted La$_{2-x}$Sr$_{x}$Cu$_{1-y}$Zn$_y$O$_4$ ($x=0.25$, $y\approx0.01$) are measured via inelastic neutron scattering techniques. As Zn$^{2+}$ is substituted onto the Cu$^{2+}$-sites, an anomalous enhancement of the local spin susceptibility $\chi^{\prime\prime}(\omega)$ appears due to the emergence of a commensurate antiferromagnetic excitation centered at wave vector \textbf{Q}$=(\pi, \pi, 0)$ that coexists with the known incommensurate SDW excitations at \textbf{Q}$_{HK}=(\pi\pm\delta,\pi), (\pi,\pi\pm\delta)$. Our results support a picture of Zn-induced antiferromagnetic (AF) fluctuations appearing through a local staggered polarization of Cu$^{2+}$-spins, and the simultaneous suppression of T$_c$ as AF fluctuations are slowed in proximity to Zn-impurities suggests the continued importance of high energy AF fluctuations at the far overdoped edge of superconductivity in the cuprates.Comment: 10 pages, 8 figure

Prevalence of Postpartum Family Planning Service Coverage in Selected Referral Facilities of Nepal

Introduction: Nepal Society of Obstetricians and Gynecologists jointly with the Nepalese government and with the support from the International Federation of Obstetrics and Gynecology has implemented an initiative to institutionalize postpartum family planning services in selected major referral facilities of Nepal to address the gap of low uptake of postpartum family planning in Nepal. The aim of the study is to find the prevalence of the service coverage of postpartum contraception in the selected facilities. Methods: A descriptive cross-sectional study was conducted in seven major referral facilities across Nepal. Data were collected from the hospital records of all women who delivered in these facilities between October 2018 and March 2019. Ethical approval for this study was obtained from Nepal Health Research Council. Data analysis was done with SPSS version 23. Results: Among the 29,072 deliveries from all the facilities, postpartum family planning counseling coverage was 27,301 (93.9%). The prevalence of uptake of Postpartum Intrauterine Device is 1581 (5.4%) and female sterilization is 1830 (6.3%). In total 11387 mothers (52.2%) had the intention to choose a postpartum family planning method. However, 36% of mothers neither used nor had the intention to choose a postpartum family planning method. Conclusions: The coverage of Postpartum Intrauterine Device counseling service coverage in Nepal is higher in 2018 as compared to 2016-2017 and in other countries implementing Postpartum Intrauterine Device initiatives. However, the prevalence of service coverage of immediate Postpartum Family Planning methods, mainly Postpartum Intrauterine Device in 2018 is lower in Nepal as compared to 2016-2017, and other countries implementing Postpartum Intrauterine Device initiative. More efforts are needed to encourage mothers delivering in the facilities to use the postpartum family planning method