The 35S U5 snRNP is generated from the activated spliceosome during In vitro splicing

Primary gene transcripts of eukaryotes contain introns, which are removed during processing by splicing machinery. Biochemical studies In vitro have identified a specific pathway in which introns are recognised and spliced out. This occurs by progressive formation of spliceosomal complexes designated as E, A, B, and C. The composition and structure of these spliceosomal conformations have been characterised in many detail. In contrast, transitions between the complexes and the intermediates of these reactions are currently less clear. We have previously isolated a novel 35S U5 snRNP from HeLa nuclear extracts. The protein composition of this particle differed from the canonical 20S U5 snRNPs but was remarkably similar to the activated B* spliceosomes. Based on this observation we have proposed a hypothesis that 35S U5 snRNPs represent a dissociation product of the spliceosome after both transesterification reactions are completed. Here we provide experimental evidence that 35S U5 snRNPs are generated from the activated B* spliceosomes during In vitro splicing

Study in optics Zeeman effect

We consider the lab to study the Zeeman effect in the optics laboratory, delivered on the basis of the authors' computer model.Рассматривается лабораторная работа по изучению эффекта Зеемана в лаборатории оптики, поставленная на основе разработанной авторами компьютерной модели

Organization of the educational process using possibilities of educational and methodical site

Teaching of General Physics at Chelyabinsk State University a number of years goes by "mixed" system of training and is based on the opportunities offered by the developed world educational and methodical site "Physicists teachers and students" (http://teachmen.ru). In this paper we attempt to summarize the work done and considers the experience of practical solutions to some problems computer didactics.Преподавание общей физики в Челябинском государственном университете опирается на возможности, предоставляемые разработанным нами учебно-методическим сайтом «Физикам преподавателям и студентам» (http://teachmen.ru). В настоящей работе сделана попытка обобщения проделанной работы, и рассматривается опыт практического решения некоторых проблем компьютерной дидактики

The RNA helicase ​Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes.

Aquarius is a multifunctional putative RNA helicase that binds precursor-mRNA introns at a defined position. Here we report the crystal structure of human Aquarius, revealing a central RNA helicase core and several unique accessory domains, including an ARM-repeat domain. We show that Aquarius is integrated into spliceosomes as part of a pentameric intron-binding complex (IBC) that, together with the ARM domain, cross-links to U2 snRNP proteins within activated spliceosomes; this suggests that the latter aid in positioning Aquarius on the intron. Aquarius's ARM domain is essential for IBC formation, thus indicating that it has a key protein-protein-scaffolding role. Finally, we provide evidence that Aquarius is required for efficient precursor-mRNA splicing in vitro. Our findings highlight the remarkable structural adaptations of a helicase to achieve position-specific recruitment to a ribonucleoprotein complex and reveal a new building block of the human spliceosome

Proposal for an Enhanced Optical Cooling System Test in an Electron Storage Ring

We are proposing to test experimentally the new idea of Enhanced Optical Cooling (EOC) in an electron storage ring. This experiment will confirm new fundamental processes in beam physics and will demonstrate new unique possibilities with this cooling technique. It will open important applications of EOC in nuclear physics, elementary particle physics and in Light Sources (LS) based on high brightness electron and ion beams.Comment: 21 pages, pdf. The number of electrons in the bunch in the example is decreased, the volume of the paper is increased, Misprints are correcte

Проблемы переподготовки операторов станков с ЧПУ в условиях центра подготовки и переподготовки кадров

Статья посвящена проблемам переподготовки операторов станков с ЧПУ, в условиях центра подготовки и переподготовки кадров на машиностроительном предприятии «Корпорации Казахмыс».The article is devoted to problems of training operators of CNC machine tools, in the center of preparation and retraining of personnel of machine-building enterprise "Kazakhmys Corporation"