179 research outputs found
Mobile resistome of human gut and pathogen drives anthropogenic bloom of antibiotic resistance
BACKGROUND:The impact of human activities on the environmental resistome has been documented in many studies, but there remains the controversial question of whether the increased antibiotic resistance observed in anthropogenically impacted environments is just a result of contamination by resistant fecal microbes or is mediated by indigenous environmental organisms. Here, to determine exactly how anthropogenic influences shape the environmental resistome, we resolved the microbiome, resistome, and mobilome of the planktonic microbial communities along a single river, the Han, which spans a gradient of human activities. RESULTS:The bloom of antibiotic resistance genes (ARGs) was evident in the downstream regions and distinct successional dynamics of the river resistome occurred across the spatial continuum. We identified a number of widespread ARG sequences shared between the river, human gut, and pathogenic bacteria. These human-related ARGs were largely associated with mobile genetic elements rather than particular gut taxa and mainly responsible for anthropogenically driven bloom of the downstream river resistome. Furthermore, both sequence- and phenotype-based analyses revealed environmental relatives of clinically important proteobacteria as major carriers of these ARGs. CONCLUSIONS:Our results demonstrate a more nuanced view of the impact of anthropogenic activities on the river resistome: fecal contamination is present and allows the transmission of ARGs to the environmental resistome, but these mobile genes rather than resistant fecal bacteria proliferate in environmental relatives of their original hosts. Video abstract
Development of a standardized in-hospital cardiopulmonary resuscitation set-up
Objective. This study evaluated whether
chest compression in a standardized inhospital
cardiopulmonary resuscitation
(CPR) set-up can be performed as effectively
as when the rescuer is kneeling beside
the patient lying on the floor. Specifically,
the in-hospital test was standardized
according to the rescuersâ average knee
height.
Methods. Experimental intervention (test
1) was a standardized, in-hospital CPR
set-up: first, the bed height was fixed at 70
cm. Second, the height difference between
the bed and a step stool was set to the average
knee height of the CPR team members
(45 cm). Control intervention (test 2) was
kneeling on floor. Thirty-eight medical
doctors on the CPR team each performed
2 minutes of chest compressions in test 1
and 2 in random order (cross-over trial). A
Little Anne was used as a simulated patient
who had experienced cardiac arrest. Chest
compression parameters, such as average
depth and rate, were measured using an accelerometer
device.
Results. In all tests, the average depths were
those recommended in the most recent
CPR guidelines (50â60 mm); there were no
significant differences between Tests 1 and
2 (53.1 ± 4.3 mm vs. 52.6 ± 4.8 mm, respectively;
p = 0.398). The average rate in Test
2 (119.1 ± 12.4 numbers/min) was slightly
faster than that in Test 1 (116.4 ± 10.2 numbers/
min; p = 0.028). No differences were
observed in any other parameters.
Conclusions. Chest compression quality in
our standardized in-hospital CPR set-up
was similar with that performed in a kneeling
position on the floor.
Trial Registration: Clinical Research Information
Service: KCT000159
Penetrating Carotid Artery Injuries Treated by an Urgent Endovascular Stent Technique: Report of Two Cases
Penetrating neck injuries are potentially dangerous and require emergent management because of the presence of vital structures in the neck. Penetrating vascular trauma to zone I and III of the neck is potentially life-threatening. An accurate diagnosis and adequate surgical intervention are critical to the successful outcome of penetrating trauma in the neck. We experienced some cases with externally penetrating injuries in neck zone II in which the patients were confirmed to have the presence of large vessel injuries in neck zones I and III. Here we report on the endovascular stent techniques used in two cases to address penetrating carotid artery injuries and review the literature
Momentum-dependent magnon lifetime in the metallic non-collinear triangular antiferromagnet CrB2
Non-collinear magnetic order arises for various reasons in several magnetic
systems and exhibits interesting spin dynamics. Despite its ubiquitous
presence, little is known of how magnons, otherwise stable quasiparticles,
decay in these systems, particularly in metallic magnets. Using inelastic
neutron scattering, we examine the magnetic excitation spectra in a metallic
non-collinear antiferromagnet CrB, in which Cr atoms form a triangular
lattice and display incommensurate magnetic order. Our data show intrinsic
magnon damping and continuum-like excitations that cannot be explained by
linear spin wave theory. The intrinsic magnon linewidth shows
very unusual momentum dependence, which our analysis shows to originate from
the combination of two-magnon decay and the Stoner continuum. By comparing the
theoretical predictions with the experiments, we identify where in the momentum
and energy space one of the two factors becomes more dominant. Our work
constitutes a rare comprehensive study of the spin dynamics in metallic
non-collinear antiferromagnets. It reveals, for the first time, definite
experimental evidence of the higher-order effects in metallic antiferromagnets.Comment: 6 pages, 4 figures, accepted for publication in PR
Spontaneous Carotid Cavernous Fistula in a Case with Protein S Deficiency that Newly Developed Ophthalmoplegia after Embolization
Clinical Implications of Residual Urine in Korean Benign Prostatic Hyperplasia (BPH) Patients: A Prognostic Factor for BPH-Related Clinical Events
Purpose Although post-void residual urine (PVR) is frequently utilized clinically in patients with benign prostatic hyperplasia (BPH), mainly because of its procedural simplicity, its role as a clinical prognostic factor, predictive of treatment goals, is still under much dispute. We investigated the predictive value of PVR for BPH-related clinical events including surgery, acute urinary retention (AUR), and admission following urinary tract infection (UTI). Methods From January to June of 2006, patients over 50 years of age who were diagnosed with BPH for the first time at the outpatient clinic and were then treated for at least 3 years with medications were enrolled in this study. The variables of patients who underwent surgical intervention for BPH, had occurrences of AUR, or required admission due to UTI (Group 1, n=43) were compared with those of patients who were maintained with medications only (Group 2, n=266). Results Group 1 had a significantly higher PVR, more severe symptoms, and a larger prostate at the time of the initial diagnosis in both the univariate and the multivariate analysis. In the 39 patients who underwent BPH-related surgery, although there was a significant change in Qmax at the time of surgery (mean, 13.1 months), PVR and the symptom score remained unchanged compared with the initial evaluation. In the receiver-operating characteristic curve analysis, the area under the curve of Group 1 was in the order of prostate volume (0.834), PVR (0.712), and symptom score (0.621). When redivided by arbitrarily selected PVR cutoffs of 50 mL, 100 mL, and 150 mL, the relative risk of clinical BPH progression was measured as 3.93, 2.61, and 2.11. Conclusions These data indicate that, in the symptomatic Korean population, increased PVR at baseline is a significant indicator of BPH-related clinical events along with increased symptom score or prostate volume
Comparison of clinical outcomes and hospital cost between open appendectomy and laparoscopic appendectomy
TCTAP A-152 Impact of Final Kissing Balloon Inflation After Simple Stent Implantation for the Treatment of Non-left Main True Coronary Bifurcation Lesions in Patients with Acute Coronary Syndrome
Comparative analysis of 7 short-read sequencing platforms using the Korean Reference Genome: MGI and Illumina sequencing benchmark for whole-genome sequencing
Background: DNBSEQ-T7 is a new whole-genome sequencer developed by Complete Genomics and MGI using DNA nanoball and combinatorial probe anchor synthesis technologies to generate short reads at a very large scale-up to 60 human genomes per day. However, it has not been objectively and systematically compared against Illumina short-read sequencers. Findings: By using the same KOREF sample, the Korean Reference Genome, we have compared 7 sequencing platforms including BGISEQ-500, DNBSEQ-T7, HiSeq2000, HiSeq2500, HiSeq4000, HiSeqX10, and NovaSeq6000. We measured sequencing quality by comparing sequencing statistics (base quality, duplication rate, and random error rate), mapping statistics (mapping rate, depth distribution, and percent GC coverage), and variant statistics (transition/transversion ratio, dbSNP annotation rate, and concordance rate with single-nucleotide polymorphism [SNP] genotyping chip) across the 7 sequencing platforms. We found that MGI platforms showed a higher concordance rate for SNP genotyping than HiSeq2000 and HiSeq4000. The similarity matrix of variant calls confirmed that the 2 MGI platforms have the most similar characteristics to the HiSeq2500 platform. Conclusions: Overall, MGI and Illumina sequencing platforms showed comparable levels of sequencing quality, uniformity of coverage, percent GC coverage, and variant accuracy; thus we conclude that the MGI platforms can be used for a wide range of genomics research fields at a lower cost than the Illumina platforms
A Prospective Population-based Study of Total Nasal Resistance in Korean Subjects
ObjectivesRhinomanometry is a widely accepted method for objective assessment of nasal patency. However, few studies have reported the values of otherwise healthy population for nasal resistance in East Asians. The purpose of this study was to measure normal total nasal resistance (TNR) values in a large sample of Korean adults and to reveal parameters contributing to TNR values.MethodsSubjects were enrolled from a cohort of the Korean Genome and Epidemiology Study. They were evaluated by anthropometry, questionnaire, and active anterior rhinomanometry at transnasal pressures of 100 and 150 Pascal (Pa).ResultsThe study sample consisted of 2,538 healthy subjects (1,298 women and 1,240 men) aged 20 to 80 years. Normal reference TNR values were 0.19±0.08 Pa/cm3/second at 100 Pa and 0.22±0.09 Pa/cm3/second at 150 Pa. The TNR of women was significantly higher than that of men (P<0.0001). TNR decreased with increasing age in both genders (P<0.05). In women, lower body weight was related to increasing TNR. In men, current smokers had higher TNR than ex-smokers and never smokers.ConclusionThe results of the present study provide information regarding the values of otherwise healthy population of TNR and parameters associated with TNR in Korean adults
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