Management and Population Status of Kihansi Spray Toad Nectophrynoides asperginis in Captive Breeding Facilities in Tanzania

Kihansi spray toad Nectophrynoides asperginis was discovered in 1996 in Kihansi gorge. The toad was declared extinct in the wild in 2009 due to habitat alteration. Before its extinction, 499 individuals were flown to the United States of America zoos in 2000 for captive breeding. In 2010, the University of Dar es Salaam, and in 2011 the Kihansi captive breeding facilities were established in Tanzania. The intention was to breed, and reintroduce the toad back to the natural environment in the gorge. The founder populations for the two facilities were sourced from the USA zoos. This study addresses captive management practices, and population status of the toad at the two facilities from 2013 to 2019. Toad counts were carried out once every two months. There was a significant increase in the number of toads over the years. The increase was associated with suitable management practices. Success in captive breeding at the facilities led to the removal of 3,236 individuals from the University for restocking the Kihansi facility and for reintroduction, and 1,273 individuals from Kihansi facility for reintroduction. The establishment of the two facilities played a major role in both the ex-situ and in-situ conservation of the toadKeywords: Kihansi spray toad; extinction; captive breeding; population; reintroduction

WHO guidelines for antimicrobial treatment in children admitted to hospital in an area of intense Plasmodium falciparum transmission: prospective study

Objectives To assess the performance of WHO’s “Guidelines for care at the first-referral level in developing countries” in an area of intense malaria transmission and identify bacterial infections in children with and without malaria

Invasive Salmonellosis among Children Admitted to a Rural Tanzanian Hospital and a Comparison with Previous Studies

BACKGROUND: The importance of invasive salmonellosis in African children is well recognized but there is inadequate information on these infections. We conducted a fever surveillance study in a Tanzanian rural hospital to estimate the case fraction of invasive salmonellosis among pediatric admissions, examine associations with common co-morbidities and describe its clinical features. We compared our main findings with those from previous studies among children in sub-Saharan Africa. METHODOLOGY/PRINCIPAL FINDINGS: From 1 March 2008 to 28 Feb 2009, 1,502 children were enrolled into the study. We collected clinical information and blood for point of care tests, culture, and diagnosis of malaria and HIV. We analyzed the clinical features on admission and outcome by laboratory-confirmed diagnosis. Pathogenic bacteria were isolated from the blood of 156 (10%) children, of which 14 (9%) were S. typhi, 45 (29%) were NTS and 97 (62%) were other pathogenic bacteria. Invasive salmonellosis accounted for 59/156 (38%) bacteremic children. Children with typhoid fever were significantly older and presented with a longer duration of fever. NTS infections were significantly associated with prior antimalarial treatment, malarial complications and with a high risk for death. CONCLUSIONS/SIGNIFICANCE: Invasive salmonellosis, particularly NTS infection, is an important cause of febrile disease among hospitalized children in our rural Tanzanian setting. Previous studies showed considerable variation in the case fraction of S. typhi and NTS infections. Certain suggestive clinical features (such as older age and long duration of fever for typhoid whereas concomitant malaria, anemia, jaundice and hypoglycemia for NTS infection) may be used to distinguish invasive salmonellosis from other severe febrile illness

Facility-based studies of invasive salmonellosis in children in sub-Saharan Africa (arranged by study site and chronologic order).

<p>Facility-based studies of invasive salmonellosis in children in sub-Saharan Africa (arranged by study site and chronologic order).</p

Clinical features on admission and outcome of febrile cases, by non-mutually exclusive laboratory-confirmed groups^*.

<p>*Comparisons of categorical data were made using the Chi square or Fishers' Exact test, as appropriate. Comparisons of continuous data were made using student's t-test for data with equal variance or Welch's t-test for those with unequal variance.</p

The study population.

<p>The study population.</p

Bacterial species isolated from 156 children with bacteremia, ranked^* according to frequency.

<p>*Rank (by age group and overall) was the same for organisms with the same frequency.</p><p>**Species included: Candida (n = 1), Citrobacter braakii (n = 1), Haemophilus parainfluenzae (n = 2), Pantoea species (n = 1), Gram negative rods not identified (n = 6).</p><p>***Species included: Bacillus (n = 19), Diphtheroids (n = 6), Micrococcus (n = 6), alpha-hemolytic Streptococcus viridans (n = 3), coagulase negative Staphylococcus (n = 98), yeast (n = 5), mixed bacterial species (n = 4), Gram positive rods not identified (n = 1).</p