Biochemical pathways triggered by antipsychotics in human oligodendrocytes: potential of discovering new treatment targets

Schizophrenia is a psychiatric disorder that affects more than 21 million people worldwide. It is an incurable disorder and the primary means of managing symptoms is through administration of pharmacological treatments, which consist heavily of antipsychotics. First-generation antipsychotics have the properties of D-2 receptor antagonists. Second-generation antipsychotics are antagonists of both D-2 and 5HT(2) receptors. Recently, there has been increasing interest in the effects of antipsychotics beyond their neuronal targets and oligodendrocytes are one of the main candidates. Thus, our aim was to evaluate the molecular effects of typical and atypical drugs across the proteome of the human oligodendrocyte cell line, MO3.13. For this, we performed a mass spectrometry-based, bottom-up shotgun proteomic analysis to identify differences triggered by typical (chlorpromazine and haloperidol) and atypical (quetiapine and risperidone) antipsychotics. Proteins which showed changes in their expression levels were analyzed in silico using Ingenuity (R) Pathway Analysis, which implicated dysregulation of canonical pathways for each treatment. Our results shed light on the biochemical pathways involved in the mechanisms of action of these drugs, which may guide the identification of novel biomarkers and the development of new and improved treatments10CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP460289/2014-42015/23049-0; 2017/18242-1; 2014/14881-1; 2014/10068-4; 2017/25588-1; 2018/03673-1CB-T, VdA, JC, and DM-d-S were supported by FAPESP (São Paulo Research Foundation), grants 2015/23049-0, 2017/18242-1, 2014/14881-1, 2014/10068-4, 2017/25588-1, and 2018/03673-1. DM-d-S was also supported by The Brazilian National Council for Scientific and Technological Development (CNPq), grant 460289/2014-4 and Serrapilheira Institute, grant number Serra-1709-1634

Spatiotemporal distribution of the magnetotactic multicellular prokaryote Candidatus Magnetoglobus multicellularis in a Brazilian hypersaline lagoon and in microcosms

Candidatus Magnetoglobus multicellularis is an unusual morphotype of magnetotactic prokaryotes. These microorganisms are composed of a spherical assemblage of gram-negative prokaryotic cells capable of swimming as a unitaligned along a magnetic field. While they occur in many aquatic habitats around the world, high numbers of Ca. M. multicellularishave been detected in Araruama Lagoon, a large hypersaline lagoon near the city of Rio de Janeiro, in Brazil. Here,we report on the spatiotemporal distribution of one such population in sediments of Araruama Lagoon, including its annualdistribution and its abundance compared with the total bacterial community. In microcosm experiments, Ca. M. multicellulariswas unable to survive for more than 45 days: the population density gradually decreased coinciding with a shift to theupper layers of the sediment. Nonetheless, Ca. M. multicellularis was detected throughout the year in all sites studied. Changes in the population density seemed to be related to the input of organic matter as well as to salinity. The populationdensity of Ca. M. multicellularis did not correlate with the total bacterial counts; instead, changes in the microbial communitystructure altered their counts in the environment. [Int Microbiol 2012; 15(3):141-149

Diversidade de Coccinellidae (Coleoptera) em plantas aromáticas (Apiaceae) como sítios de sobrevivência e reprodução em sistema agroecológico.

Studies show that Apiaceae may provide concentrated vital resources for predator insects,stimulating their abundance, diversity and persistence in agricultural systems, thereby increasing their effi ciency as biological control agents. Among the predatory insects, Coccinellidae (Coleoptera) on many different species both as larvae and adults, complementing their diet with pollen and/or nectar. This study aimed to determine the diversity and relative abundance of Coccinellidae species visiting plants of Anethum graveolens (dill), Coriandrum sativum (coriander) and Foeniculum vulgare (sweet fennel) (all Apiaceae), particularly in their blooming seasons, and to evaluate the potential of these aromatic species for providing the resources for survivorship and reproduction of coccinelids. Coccinellids were collected by removal of samplings from September to October, 2007. Besides one unidentifi ed species of Chilocorinae, five species of Coccinellinae were collected: Coleomegilla maculata DeGeer, Coleomegilla quadrifasciata (Schönherr), Cycloneda sanguinea (L.), Eriopis connexa (Germar) and Hippodamia convergens Guérin-Meneville. Dill provided a signifi cant increase in the abundance of coccinellids as compared to coriander and sweet fennel. These aromatic species were used by coccinellids as survival and reproduction sites, providing food resources (pollen and/or prey), shelter for larvae, pupae and adults, and mating and oviposition sites as well

The effects of cyclosporin a and heteropterys tomentosa on the rat liver

Cyclosporin A (CsA) is a widely employed immunosuppressive drug that is associated with several side effects, among then hepatotoxicity. Heteropterys tomentosa is a Brazilian plant efficient in reducing damage caused by CsA on the rat testis and prostate. The aim of this study was to evaluate the effect of CsA and H. tomentosa (administered isolated or simultaneously) on the liver of Wistar rats. The animals were treated daily with water (control), CsA (15mg/kg/day), H. tomentosa infusion or CsA+H. tomentosa, for 21 or 56 days. The treatments did not alter liver morphology or cause fibrosis. H. tomentosa administered for 21 days increased the number of hepatocyte nuclei and Kupffer cell volumetric proportion. After 56 days of treatment, H. tomentosa administration did not alter the parameters analyzed. Biochemical plasma dosages and liver stereology showed impairment caused by CsA-treatment after 21 days; these results were not observed after 56 days of treatment. The simultaneous treatment with CsA and H. tomentosa for 21 or 56 days did not alleviate nor accentuate CsA hepatic effects. The present study showed that the 21 days treatment with CsA caused more alteration to the liver than the 56 days treatment; this could be related to hepatic recovery after the long term treatment871369379FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP2011/01160-

The Effects Of Cyclosporin A And Heteropterys Tomentosa On The Rat Liver.

Cyclosporin A (CsA) is a widely employed immunosuppressive drug that is associated with several side effects, among then hepatotoxicity. Heteropterys tomentosa is a Brazilian plant efficient in reducing damage caused by CsA on the rat testis and prostate. The aim of this study was to evaluate the effect of CsA and H. tomentosa (administered isolated or simultaneously) on the liver of Wistar rats. The animals were treated daily with water (control), CsA (15mg/kg/day), H. tomentosa infusion or CsA+H. tomentosa, for 21 or 56 days. The treatments did not alter liver morphology or cause fibrosis. H. tomentosa administered for 21 days increased the number of hepatocyte nuclei and Kupffer cell volumetric proportion. After 56 days of treatment, H. tomentosa administration did not alter the parameters analyzed. Biochemical plasma dosages and liver stereology showed impairment caused by CsA-treatment after 21 days; these results were not observed after 56 days of treatment. The simultaneous treatment with CsA and H. tomentosa for 21 or 56 days did not alleviate nor accentuate CsA hepatic effects. The present study showed that the 21 days treatment with CsA caused more alteration to the liver than the 56 days treatment; this could be related to hepatic recovery after the long term treatment.87369-7

In2CoP 2020 - International Conference on Co-Creation Processes in Higher Education 2020: book of abstracts

A Cocriação e a Inovação no Ensino Superior representam um dos principais compromissos do Instituto Politécnico de Bragança (IPB), cuja atividade formativa e de investigação se orienta, de forma sinérgica, para a cooperação com as empresas e instituições da região. O IPB pretende contribuir, de forma ativa, para uma economia regional baseada no conhecimento e com uma forte articulação internacional. Nesta esteira, teve lugar, na cidade de Bragança, nos dias 29 a 30 de janeiro de 2020, a Conferência Internacional em Processos de Cocriação no Ensino Superior (In2CoP). Privilegiamos um espaço de reflexão de processos e de partilha de resultados de ecossistemas de cocriação, visando o desenvolvimento de uma comunidade de aprendizagem integradora, interdisciplinar e multicultural. Ao longo dos três dias da conferência, mais de 150 conferencistas, nacionais e estrangeiros, participaram, ativamente, nas diversas atividades propostas: uma sessão plenária com seis intervenções, quatro workshops e uma sessão pitch com a apresentação de trinta e seis projetos de inovação e cocriação. Com um espírito inovador, as atividades decorreram no campus do IPB e em espaços emblemáticos da cidade de Bragança, designadamente: Centro de Arte Contemporânea Graças Morais, Centro de Fotografia Georges Dussaud e Centro de Ciência Viva. Foi ainda realizada uma visita social ao Museu do Côa em Vila Nova de Foz Côa.Co-creation and Innovation in Higher Education represents one of the main commitments of the Polytechnic Institute of Bragança (IPB), whose training and research activity is synergistically oriented towards cooperation with companies and institutions in the region. The IPB intends to contribute actively to a regional economy based on knowledge and with a strong international articulation. In this context, the International Conference on Co-Creation Processes in Higher Education (In2CoP) took place in the city of Bragança, on January 29-30, 2020. We privilege a space for reflection of processes and sharing of results from co-creation ecosystems, aiming at the development of an integrative, interdisciplinary, and multicultural learning community. Over the three days of the conference, more than 150 national and foreign conferencists actively participated in the various activities proposed: a plenary session with six interventions, four workshops, and a pitch session with the presentation of thirty-six innovation and co-creation projects. With an innovative spirit, the activities took place in IPB's campus and in emblematic spaces in the city of Bragança, namely: Centro de Arte Contemporânea Graças Morais, Centro de Fotografia Georges Dussaud and Centro de Ciência Viva. A social visit was also made to the Côa Museum in Vila Nova de Foz Côa.info:eu-repo/semantics/publishedVersio

Biochemical Pathways Triggered by Antipsychotics in Human Oligodendrocytes: Potential of Discovering New Treatment Targets

Schizophrenia is a psychiatric disorder that affects more than 21 million people worldwide. It is an incurable disorder and the primary means of managing symptoms is through administration of pharmacological treatments, which consist heavily of antipsychotics. First-generation antipsychotics have the properties of D2 receptor antagonists. Second-generation antipsychotics are antagonists of both D2 and 5HT2 receptors. Recently, there has been increasing interest in the effects of antipsychotics beyond their neuronal targets and oligodendrocytes are one of the main candidates. Thus, our aim was to evaluate the molecular effects of typical and atypical drugs across the proteome of the human oligodendrocyte cell line, MO3.13. For this, we performed a mass spectrometry-based, bottom-up shotgun proteomic analysis to identify differences triggered by typical (chlorpromazine and haloperidol) and atypical (quetiapine and risperidone) antipsychotics. Proteins which showed changes in their expression levels were analyzed in silico using Ingenuity® Pathway Analysis, which implicated dysregulation of canonical pathways for each treatment. Our results shed light on the biochemical pathways involved in the mechanisms of action of these drugs, which may guide the identification of novel biomarkers and the development of new and improved treatments

Retinoblastoma in a pediatric oncology reference center in southern brazil

Background: Retinoblastoma (Rb) is the most common intraocular tumor diagnosed in children in Brazil. However, detailed information is lacking regarding patient clinical demographics. This study aimed to determine the clinical profile of patients with Rb who were treated in a public university hospital in southern Brazil from 1983 to 2012. Methods: Patients’ medical records were reviewed to retrospectively identify patients with a principal diagnosis of Rb. Rb was classified as hereditary or non-hereditary. Clinical staging was reviewed by an ophthalmologist. Statistical analysis was performed using SPSS. Results: Of 165 patients with a diagnosis of Rb during this period, 140 were included in the study. Disease was unilateral in 65.0 % of patients, bilateral in 32.9 %, and trilateral in 2.1 %. The mean age at onset of the first sign/ symptom was 18.1 month, and 35.7 % of patients were diagnosed during the first year of life. The most common presenting signs were leukocoria (73.6 %) and strabismus (20.7 %). The mean age at diagnosis was 23.5 months, and time to diagnosis was 5.4 months. In patients with clinical features of hereditary Rb, both onset of the first sign/symptom and diagnosis were at an earlier age than in patients without these features (12.3 vs 21.6 months [P = 0.001] and 15.9 vs 28.0 months [P < 0.001], respectively). However, there was no significant difference in overall survival between the two groups. Ocular stage at diagnosis was advanced in 76.5 % (Reese V) and 78.1 % (International Classification D or E). Of patients with unilateral and bilateral disease, 35.2 % and 34.8 %, respectively, had extraocular disease at diagnosis; 10.7 % had metastatic disease at diagnosis. Enucleation was observed in 88.1 % and exenteration in 11.9 % of patients; 93.6 % patients were followed until 2012, and 22.9 % relapsed. Overall survival was 86.4 %. Conclusions: Most Rb diagnoses are still diagnosed in advanced stages of the disease, considerably reducing overall survival time and the rate of eye and vision preservation

Projetos de inovação e cocriação como fatores de valor económico e social em meio rural

A aposta na inovação nas organizações é cada vez mais, um fator chave para a sua sustentabilidade e competitividade As Instituições de Ensino Superior (têm vindo a explorar a metodologia de cocriação para a inovação entre empresas e equipas multidisciplinares de estudantes, potenciando um ambiente de inovação no território Este estudo de caso, baseia-se, fundamentalmente, na análise de conteúdo dos documentos finais de 28 projetos/desafios de cocriação o da inovação, desenvolvidos no âmbito do Programa Demola IPB, relacionados com a produção animal, produção agrícola, uso dos recursos florestais, agroindústrias e sector alimentar, e desenvolvimento rural.info:eu-repo/semantics/publishedVersio