Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera

<p>Abstract</p> <p>Background</p> <p>American Trypanosomiasis or Chagas disease is caused by <it>Trypanosoma cruzi </it>which currently infects approximately 16 million people in the Americas causing high morbidity and mortality. Diagnosis of American trypanosomiasis relies on serology, primarily using indirect immunofluorescence assay (IFA) with <it>T. cruzi </it>epimastigote forms. The closely related but nonpathogenic <it>Trypanosoma rangeli </it>has a sympatric distribution with <it>T. cruzi </it>and is carried by the same vectors. As a result false positives are frequently generated. This confounding factor leads to increased diagnostic test costs and where false positives are not caught, endangers human health due to the toxicity of the drugs used to treat Chagas disease.</p> <p>Results</p> <p>In the present study, serologic cross-reactivity between the two species was compared for the currently used epimastigote form and the more pathologically relevant trypomastigote form, using IFA and immunoblotting (IB) assays. Our results reveal an important decrease in cross reactivity when <it>T. rangeli </it>culture-derived trypomastigotes are used in IFA based diagnosis of Chagas disease. Western blot results using sera from both acute and chronic chagasic patients presenting with cardiac, indeterminate or digestive disease revealed similar, but not identical, antigenic profiles.</p> <p>Conclusion</p> <p>This is the first study addressing the serological cross-reactivity between distinct forms and strains of <it>T. rangeli </it>and <it>T. cruzi </it>using sera from distinct phases of the Chagasic infection. Several <it>T. rangeli</it>-specific proteins were detected, which may have potential as diagnostic tools.</p

Investigation of the double-lobed sources of the Cygnus constellation core

We present a collection of double-lobed sources towards a area of the Cygnus region at the northern sky, observed at 325 and 610 MHz with the Giant Metrewave Radio Telescope. The 10 resolution achieved at 325 MHz is 5.5 times better than previous studies, while at 610 MHz, these are the first results ever of such a large area, mapped with 6 angular resolution. After a thorough visual inspection of the images at the two bands, we found 43 double-lobed source candidates, proposed as such due to the presence of 2 bright peaks, within a few arcminutes apart, joined by a bridge or a central nucleus. All but two are presented here as a double-lobed candidates for the first time. Thirty nine of the candidates were covered at both bands, and we provide the spectral index information for them. We have searched for positional coincidences between the detected sources/components and other objects from the literature, along the electromagnetic spectrum. Twenty-three candidates possess radio counterpart(s), 12 present infrared counterparts, and 1 showed an overlapping X-ray source. We analysed each candidate considering morphology, counterparts, and spectral indices. Out of the 43 candidates, 37 show characteristics compatible with an extragalactic nature, 2 of probably Galactic origin, 3 remain as dubious cases, though with feature(s) compatible with an extragalactic nature, and the remaining one, evidence of physically unrelated components. The median spectral index of the 40 putative extragalactic sources is . Their celestial surface density at 610 MHz resulted in , across a region lying at the Galactic plane.Fil: Saponara, Juliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Benaglia, Paula. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Andruchow, Ileana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Ishwara Chandra, C. H.. National Centre For Radio Astrophysics; IndiaFil: Intema, H. T.. University of Western Australia; Australi

Effect of a gap on the decoherence of a qubit

We revisit the problem of the decoherence and relaxation of a central spin coupled to a bath of conduction electrons. We consider both metallic and semiconducting baths to study the effect of a gap in the bath density of states (DOS) on the time evolution of the density matrix of the central spin. We use two weak coupling approximation schemes to study the decoherence. At low temperatures, though the temperature dependence of the decoherence rate in the case of a metallic bath is the same irrespective of the details of the bath, the same is not true for the semiconducting bath. We also calculate the relaxation and decoherence rates as a function of external magnetic fields applied both on the central spin and the bath. We find that in the presence of the gap, there exists a certain regime of fields, for which surprisingly, the metallic bath has lower rates of relaxation and decoherence than the semiconducting bath.Comment: 9 pages, 9 figure

In Silico Derivation of HLA-Specific Alloreactivity Potential from Whole Exome Sequencing of Stem Cell Transplant Donors and Recipients: Understanding the Quantitative Immuno-biology of Allogeneic Transplantation

Donor T cell mediated graft vs. host effects may result from the aggregate alloreactivity to minor histocompatibility antigens (mHA) presented by the HLA in each donor-recipient pair (DRP) undergoing stem cell transplantation (SCT). Whole exome sequencing has demonstrated extensive nucleotide sequence variation in HLA-matched DRP. Non-synonymous single nucleotide polymorphisms (nsSNPs) in the GVH direction (polymorphisms present in recipient and absent in donor) were identified in 4 HLA-matched related and 5 unrelated DRP. The nucleotide sequence flanking each SNP was obtained utilizing the ANNOVAR software package. All possible nonameric-peptides encoded by the non-synonymous SNP were then interrogated in-silico for their likelihood to be presented by the HLA class I molecules in individual DRP, using the Immune-Epitope Database (IEDB) SMM algorithm. The IEDB-SMM algorithm predicted a median 18,396 peptides/DRP which bound HLA with an IC50 of <500nM, and 2254 peptides/DRP with an IC50 of <50nM. Unrelated donors generally had higher numbers of peptides presented by the HLA. A similarly large library of presented peptides was identified when the data was interrogated using the Net MHCPan algorithm. These peptides were uniformly distributed in the various organ systems. The bioinformatic algorithm presented here demonstrates that there may be a high level of minor histocompatibility antigen variation in HLA-matched individuals, constituting an HLA-specific alloreactivity potential. These data provide a possible explanation for how relatively minor adjustments in GVHD prophylaxis yield relatively similar outcomes in HLA matched and mismatched SCT recipients.Comment: Abstract: 235, Words: 6422, Figures: 7, Tables: 3, Supplementary figures: 2, Supplementary tables:

CD47 expression is decreased in hematopoietic progenitor cells in patients with myelofibrosis

Myelofibrosis (MF) is characterized by increased circulating hematopoietic progenitor cells (HPCs), abnormal cytokine levels, and the survival advantage of neoplastic progenitors over their normal counterparts, which leads to progressive disappearance of polyclonal hematopoiesis. CD47 is a surface glycoprotein with many functions, such as acting as a phagocytosis inhibitor of the expressing cell, that is increased in normal hematopoietic stem and progenitor cells mobilized into the blood and several human cancer-initiating cells, such as in acute myeloid leukemia. We compared CD47 expression in hematopoietic stem and progenitor cells of patients with MF and controls and found it to be decreased in progenitors of MF. Exposure of control HPCs to the cytokines transforming growth factor β and stromal-derived factor 1, which are important regulators of hematopoietic stem cell cycling and are overexpressed in patients with MF, did not modulate CD47 expression

Position of the mandibular foramen in different facial shapes assessed by cone-beam computed tomography: a cross-sectional retrospective study

Purpose: The mandibular foramen, located on the internal surface of the mandibular ramus, is an important anatomical landmark for the success during the inferior alveolar nerve block. This cross-sectional retrospective study aimed to evaluate the location of the mandibular foramen through ConeBeam Computed Tomography (CBCT) in different facial shapes. Materials and Methods: The determination of the location of the mandibular foramen was performed using CBCT of mesocephalic, dolichocephalic and brachycephalic patients (n=40 each). The ramus width (W), the distance from the mandibular foramen to the deepest point of the anterior border of the mandibular ramus (D), the distance from the mandibular foramen to the lowest point of the mandibular notch (V) and the distance from the inferior border of the mandible to the lowest point in of the mandibular border (R), as well as the ratios W/D and V/R, were measured. ANCOVA, two-way ANOVA and Chi-square tests were used to analyze the variation among the facial shapes. Results.: The ramus width (W) was greater (p 0.0001) in the brachycephalic (28.4 +/- 0.5 mm) than in both mesocephalic (26.8 +/- 0.36 mm) and dolichocephalic (25.5 +/- 0.39 mm) patients. D (p=0.0433) and R (p=0.0072) were also greater in the brachycephalic (17.7 +/- 0.36 mm; 43.4 +/- 0.75 mm, respectively) than dolichocephalic (16.5 +/- 03 mm; 40.3 +/- 0.63 mm, respectively), but both did not differ from mesocephalic (17.34.36 mm; 41.8 +/- 0.66 mm, respectively) patients. The other measurements (V, W/J and R/V) did not significantly differ among facial shapes. Conclusion: The localization of the mandibular foramen was. in the horizontal direction, more posterior in the brachycephalic patients and, in the vertical direction, higher in the dolichocephalic patients, when compared to the other groups analyzed. Thus, the anatomic data found in this study may help dentists to increase the success of the inferior alveolar nerve block and prevent surgical complications.1354455