A Study on the Tendency of the Environmental Education in Geography Text-book of the World

1.帝国書院から翻訳出版された世界地理教科書全30巻を通読し, そのなかから国情の差と世界全域を網羅することを考慮して23ヶ国を選び, 地理教育における環境教育の一側面を捉える試みを行った.2.対象とした各国の教科書は, それぞれの国の教育制度, 教育年限, 教育事情が異なるため, 対象年令が若干異なっているが, 一部を除き大半は中等教育以上の教科書である.次代を教育する教科書という共通の基盤にたつものと理解して, 以下の調査を行い若干の知見を得た.3.各国の地理教科書から環境と人間活動とに係る環境問題に関する記述を抽出してまず記述量を把握し, 次いで記述の対象について13項目に分け, さらに42の細目に分類整理した.その結果は第1図, 第2表, 第4表に示した.4.抽出した記述量は全体で566記述, 単純な一国平均は25記述となり, 最高記述国は56記述のアメリカ合衆国であった.5.さらに記述量が多い項目第1位と第2位を基にして, 各国の特色を把握し第3表のような5グループに分類した.6. 42種に細分した記述からは, 各国それぞれの環境問題に対する関心の領域の広狭をうかがうことができるが, 最も広範にわたって記述している国は記述量でも多いアメリカ合衆国であった.7.記述量と記述細目数とを基にすると地理教育の分野で, 環境教育問題に関心を寄せている国とさほどではない国とに分かれる.関心が高い国と察知されるのは, アメリカ合衆国, フランス, オーストラリアなど10ヶ国であった.8.環境教育問題は, 執筆形式にもよるが, 単元 (章) や節を直接設けている関心が高い国は, 第5表のとおり10ヶ国見られた.9.環境教育の方法, 環境問題の中心に取り上げられている課題あるいは視点は, 国情によってIn this study, we made it clear that tendency of environmental education in the geography textbook of the world. We used textbooks in Japanese translation of each country languages and these textbooks timely published by the Teikoku-Shoin from 1979 to 1980. The method of study is as follows. The environmental problems such as natural conservation and environment, various field of industry, various kinds of industrial pollution, living environment, outdoor recreation and other things are extracted from the discription in textbooks. We counted out the number of extracted data each country and matters for investigation and made a surveyed of its character in the same time. These data was classified into thirteen groups according to the same factor and subdivided into forty two items such as protection and conservation area, pollution, natural resources, living environment, municipal waste, land development and other things. A summary of the survey is shown below. 1) The count of environmental discription in each country range from the most fifty three in U. S. A. to the lowest five in Mexco and Brazil and its average twenty five. 2) As we cannot easily compare with that each country has its own writing style of textbook, it was ten countries that take up a environmental problems by chapter or section on the textbook. 3) Each country it different from politic and economic situation, framework of society, standard of land development and living and so on. The character of each country from a point of view environmental problems and its education can be broadly classified into five group. 4) Moreover, we was classified by character of environmental education as developed country and developing country and the former may be divied into a narrow, overpopulated old country and a wide, abundant in natural resources newer country

Genome-wide Map of Nucleosome Acetylation and Methylation in Yeast

SummaryEukaryotic genomes are packaged into nucleosomes whose position and chemical modification state can profoundly influence regulation of gene expression. We profiled nucleosome modifications across the yeast genome using chromatin immunoprecipitation coupled with DNA microarrays to produce high-resolution genome-wide maps of histone acetylation and methylation. These maps take into account changes in nucleosome occupancy at actively transcribed genes and, in doing so, revise previous assessments of the modifications associated with gene expression. Both acetylation and methylation of histones are associated with transcriptional activity, but the former occurs predominantly at the beginning of genes, whereas the latter can occur throughout transcribed regions. Most notably, specific methylation events are associated with the beginning, middle, and end of actively transcribed genes. These maps provide the foundation for further understanding the roles of chromatin in gene expression and genome maintenance

Stochastic mRNA production by a three-state gene

We consider a model of mRNA production governed by the dynamics of a gene that exists in three possible states -- inactive, poised and active. The transitions between the adjacent states are controlled by stochastic processes characterized by corresponding on/off rates. mRNA is produced only when the gene is in active state and we also consider mRNA denaturation leading to its death.Comment: 7 pages, 3 figure

TATA and paused promoters active in differentiated tissues have distinct expression characteristics

Abstract Core promoter types differ in the extent to which RNA polymerase II (Pol II) pauses after initiation, but how this affects their tissue‐specific gene expression characteristics is not well understood. While promoters with Pol II pausing elements are active throughout development, TATA promoters are highly active in differentiated tissues. We therefore used a genomics approach on late‐stage Drosophila embryos to analyze the properties of promoter types. Using tissue‐specific Pol II ChIP‐seq, we found that paused promoters have high levels of paused Pol II throughout the embryo, even in tissues where the gene is not expressed, while TATA promoters only show Pol II occupancy when the gene is active. The promoter types are associated with different chromatin accessibility in ATAC‐seq data and have different expression characteristics in single‐cell RNA‐seq data. The two promoter types may therefore be optimized for different properties: paused promoters show more consistent expression when active, while TATA promoters have lower background expression when inactive. We propose that tissue‐specific genes have evolved to use two different strategies for their differential expression across tissues

Highly Contiguous Genome Assemblies of 15 Drosophila Species Generated Using Nanopore Sequencing

The Drosophila genus is a unique group containing a wide range of species that occupy diverse ecosystems. In addition to the most widely studied species, Drosophila melanogaster, many other members in this genus also possess a well-developed set of genetic tools. Indeed, high-quality genomes exist for several species within the genus, facilitating studies of the function and evolution of cis-regulatory regions and proteins by allowing comparisons across at least 50 million years of evolution. Yet, the available genomes still fail to capture much of the substantial genetic diversity within the Drosophila genus. We have therefore tested protocols to rapidly and inexpensively sequence and assemble the genome from any Drosophila species using single-molecule sequencing technology from Oxford Nanopore. Here, we use this technology to present highly contiguous genome assemblies of 15 Drosophila species: 10 of the 12 originally sequenced Drosophila species (ananassae, erecta, mojavensis, persimilis, pseudoobscura, sechellia, simulans, virilis, willistoni, and yakuba), four additional species that had previously reported assemblies (biarmipes, bipectinata, eugracilis, and mauritiana), and one novel assembly (triauraria). Genomes were generated from an average of 29x depth-of-coverage data that after assembly resulted in an average contig N50 of 4.4 Mb. Subsequent alignment of contigs from the published reference genomes demonstrates that our assemblies could be used to close over 60% of the gaps present in the currently published reference genomes. Importantly, the materials and reagents cost for each genome was approximately $1,000 (USD). This study demonstrates the power and cost-effectiveness of long-read sequencing for genome assembly in Drosophila and provides a framework for the affordable sequencing and assembly of additional Drosophila genomes

Data from: A global change in RNA polymerase II pausing during the Drosophila midblastula transition

Massive zygotic transcription begins in many organisms during the midblastula transition when the cell cycle of the dividing egg slows down. A few genes are transcribed before this stage but how this differential activation is accomplished is still an open question. We have performed ChIP-seq experiments on tightly staged Drosophila embryos and show that massive recruitment of RNA polymerase II (Pol II) with widespread pausing occurs de novo during the midblastula transition. However, ∼100 genes are strongly occupied by Pol II before this timepoint and most of them do not show Pol II pausing, consistent with a requirement for rapid transcription during the fast nuclear cycles. This global change in Pol II pausing correlates with distinct core promoter elements and associates a TATA-enriched promoter with the rapid early transcription. This suggests that promoters are differentially used during the zygotic genome activation, presumably because they have distinct dynamic properties

Supplemental Material for Miller et al., 2018

<div> <div> <div> <p>The Drosophila genus is a unique group containing a wide range of species that occupy diverse ecosystems. In addition to the most widely studied species, Drosophila melanogaster, many other members in this genus also possess a well-developed set of genetic tools. Indeed, high-quality genomes exist for several species within the genus, facilitating studies of the function and evolution of cis-regulatory regions and proteins by allowing comparisons across at least 50 million years of evolution. Yet, the available genomes still fail to capture much of the substantial genetic diversity within the Drosophila genus. We have therefore tested protocols to rapidly and inexpensively sequence and assemble the genome from any Drosophila species using single-molecule sequencing technology from Oxford Nanopore. Here, we use this technology to present highly contiguous genome assemblies of 15 Drosophila species: 10 of the 12 originally sequenced Drosophila species (ananassae, erecta, mojavensis, persimilis, pseudoobscura, sechellia, simulans, virilis, willistoni, and yakuba), four additional species that had previously reported assemblies (biarmipes, bipectinata, eugracilis, and mauritiana), and one novel assembly (triauraria). Genomes were generated from an average of 29x depth-of-coverage data that after assembly resulted in an average contig N50 of 4.4 Mb. Subsequent alignment of contigs from the published reference genomes demonstrates that our assemblies could be used to close over 60% of the gaps present in the currently published reference genomes. Importantly, the materials and reagents cost for each genome was approximately $1,000 (USD). This study demonstrates the power and cost-effectiveness of long-read sequencing for genome assembly in Drosophila and provides a framework for the affordable sequencing and assembly of additional Drosophila genomes. </p> </div> </div> </div

Hippo Reprograms the Transcriptional Response to Ras Signaling

Hyperactivating mutations in Ras signaling are hallmarks of carcinomas. Ras signaling mediates cell fate decisions as well as proliferation during development. It is not known what dictates whether Ras signaling drives differentiation versus proliferation. Here we show that the Hippo pathway is critical for this decision. Loss of Hippo switches Ras activation from promoting cellular differentiation to aggressive cellular proliferation. Transcriptome analysis combined with genetic tests show that this excessive proliferation depends on the synergistic induction of Ras target genes. Using ChIP-nexus, we find that Hippo signaling keeps Ras targets in check by directly regulating the expression of two key downstream transcription factors of Ras signaling: the ETS-domain transcription factor Pointed and the repressor Capicua. Our results highlight how independent signaling pathways can impinge on each other at the level of transcription factors, thereby providing a safety mechanism to keep proliferation in check under normal developmental conditions.status: publishe

Whole-genome ChIP–chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo

Genetic studies have identified numerous sequence-specific transcription factors that control development, yet little is known about their in vivo distribution across animal genomes. We determined the genome-wide occupancy of the dorsoventral (DV) determinants Dorsal, Twist, and Snail in the Drosophila embryo using chromatin immunoprecipitation coupled with microarray analysis (ChIP–chip). The in vivo binding of these proteins correlate tightly with the limits of known enhancers. Our analysis predicts substantially more target genes than previous estimates, and includes Dpp signaling components and anteroposterior (AP) segmentation determinants. Thus, the ChIP–chip data uncover a much larger than expected regulatory network, which integrates diverse patterning processes during development