1,298 research outputs found
Reviews: Jay E. Kinsbruner, The Colonial Spanish-American City: Urban Life in the Age of Atlantic Capitalism and James Robertson, Gone is the Ancient Glory: Spanish Town, Jamaica
Book reviews of Jay E. Kinsbruner, The Colonial Spanish-American City: Urban Life in the Age of Atlantic Capitalism. Austin, TX: University of Texas Press, 2005. 198 PP·. ISBN: 0- 292-70621-9 (hbk.); James Robertson, Gone is the Ancient Glory: Spanish Town, Jamaica, 1500-2000. Kingston, Jamaica: Ian Randle Publishers, 2005. 496 PP· ISBN: 976-637-198-9 (hbk.); 976-637-197-0 (pbk.)
Hispanic Studies in Honor of Robert L. Fiore
Writing this introduction not only honors an extraordinary Hispanist but, more than anything else, a formidable professor whose work in education has reached beyond the classroom to the hallways of the department, to his home over a glass of brandy, to the local coffee shop, to long fruitful conversations in nearby Italian restaurants, to the opera, to the paddleball court or golf course, or in front of Masaccio’s frescos in Florence. Robert L. Fiore, mentor and friend, has remained the truest advisor and teacher to his students, consummate supporter to his colleagues, and loyal friend to those who know him outside academia. His wise and const ant counsel—the most precious gift he could have ever offered to his students—is exactly what he received from trust ed and faithful mentors early on in his career. Th is volume of essays by some of the most well known Hispanists today is not only proof of Bob’s impact on Golden Age studies, but also a small measure of appreciation by all who have had the good fortune to have known him, and been affect ed by him in some way. Th is introduction, surely too short and insignificant alongside Bob’s accomplishments, endeavors to put into words Bob’s lifelong contributions in and out of academia which are here remembered by those who respect and admire him.https://lib.dr.iastate.edu/language_books/1009/thumbnail.jp
A Companion to Cervantes\u27s Novelas ejemplares by Stephen Boyd
Producto en su mayoria del congreso celebrado en University College en Cork en 2001 titulado Artificio e invention: Cervantes\u27s Novelas ejemplare, este volumen es un conjunto de catorce articulos editado magistralmente por Stephen Boyd. Como consecuencia del escaso reconocimiento de las Novelas ejemplares fuera del mundo academico de los hispanistas, en su introduction Boyd declara que uno de los objetivos de la obra es precisamente el de dirigirla a un amplio publico lector angloparlante
Los escenarios de la memoria: psicodrama en El trato de Argel de Cervantes
Analizado desde la óptica de las ideas de la psicoanalista Joyce McDougall en Theaters of the Mind y el psicodrama de Jacob Levy Moreno, El trato de Argel sirve como posible e hipotético reflejo del mundo interno de Cervantes. Por medio del psicodrama, el individuo examina su persona y sus relaciones personales mediante la actuación improvisada sobre un escenario solo o con otros personajes. Existen diferentes técnicas utilizadas en el psicodrama (representaciones del yo, soliloquios, proyecciones, intercambio de papeles, personajes antagónicos, juegos de dobles o la técnica del espejo) por medio de las cuales el individuo sometido a estudio explora conflictos internos puestos en escena a través de diferentes personajes que dramatizan la experiencia. Este conjunto de técnicas utilizadas en el psicodrama se observan igualmente en El trato de Argel, donde Cervantes reconstruye su pasado dando rienda suelta a los fantasmas del cautiverio argelino. En la pieza teatral, Cervantes pone en escena una treintena de personajes antagónicos y complementarios entre sí que hacen eco de las experiencias vividas por el mismo escritor durante su cautiverio en Argel, reconstruyendo el pasado desde distintos ángulos y perspectivas. El espectador tiene ante sí un yo fragmentado a través de personajes en contradicción que buscan salida por medio de la puesta en escena y que se mueven en espacios ambivalentes y heterogéneos. El Trato de Argel se convierte en psicodrama que captura metafóricamente los conflictos internos propios de la psicología tradicional del cautivo como son los diferentes niveles del cautiverio, la tortura, la sensación de abandono por parte del gobierno y la familia, la apostasía, el rescate, la huida o el suicidio
Disposición al uso de sitios web de un grupo de organizaciones no gubernamentales (ONG) de Medellín
En la actualidad, en Colombia el uso de los sitios web en las ONG no se ha generalizado y analizar las razones de ello puede llevar a establecer estrategias de orientación hacia su uso como herramienta de comunicación para sus labores -- El presente estudio se realizó para conocer la disposición de las organizaciones no gubernamentales (ONG) afiliadas a la Federación Antioqueña de ONG (FAONG) localizadas en la ciudad de Medellín hacia el uso de sitios web -- El interés central consistió en encontrar las ventajas y desventajas que las mismas encuentran acerca de la adopción de estar presentes en el canal en línea a través del uso de sitios web -- Para ello se partió de una revisión de antecedentes teóricos que exploran la adopción de herramientas digitales en diferentes empresas, definen las ventajas y desventajas de la presencia en línea y encuentran algunos motivadores e inhibidores y el nivel de conocimiento de las entidades en cuanto al uso de internet como canal de comunicación -- Para lograr el objetivo se hizo un estudio exploratorio en el que se aplicaron instrumentos, como entrevistas y encuestas, a un grupo de informantes seleccionados de ONG afiliadas a la FAONG -- Los hallazgos mostraron el interés de las organizaciones de tener un sitio web para comunicar e informar sobre su objeto social y determinar hacia cuál población están enfocadas; también se encontraron falencias en cuanto al conocimiento, el uso y la comunicación con sus públicos frente al manejo adecuado de un sitio we
Characterization of the actin-like MreB cytoskeleton dynamics and its role in cell wall synthesis in Bacillus subtilis
The peptidoglycan cell wall (CW) and the actin-like MreB cytoskeleton are the majordeterminants of cell morphology in non-spherical bacteria. Bacillus subtilis is a rod-shaped Grampositive
bacterium that has three MreB isoforms: MreB, Mbl (MreB–like) and MreBH (MreBHomologue). Over the last decade, all three proteins were reported to localize in dynamic filamentous helical structures running the length of the cells underneath the membrane. This helical pattern led to a model where the extended MreB structures act as scaffolds to position CW-synthesizing machineries along sidewalls. However, the dynamic relationship between the MreB cytoskeleton and CW elongation complexes remained to be elucidated. Here we describe the characterization of the dynamics of the three MreB isoforms, CW synthesis and elongation complexes in live Bacillus subtilis cells at high spatial and temporal resolution. Using total internal reflection fluorescence microscopy (TIRFM) we found that MreB, Mbl and MreBH actually do not assemble into an extended helical structure but instead into discrete patches that move processively along peripheral tracks perpendicular to the long axis of the cell. We found similar patch localization and dynamics for several morphogenetic factors and CW-synthesizing enzymes including MreD, MreC, RodA, PbpH and PBP2a. Furthermore, using fluorescent recovery after photobleaching (FRAP), we showed that treadmilling of MreB filaments does not drive patch motility, as expected from the structural homology to actin. Blocking CW synthesis with antibiotics that target different steps of the peptidoglycan biosynthetic pathway stopped MreB patches motion, suggesting that CW synthesis is the driving force of patch motility. On the basis of these findings, we proposed a new model for MreB fuction in which MreB polymers
restrict and orient patch motility to ensure controlled lateral CW expansion, thereby maintaining cell shape. To further investigate the molecular mechanism underlying MreB action, we next performed a site-directed mutagenesis analysis. Alanine substitutions of three charged amino 2 acids of MreB generated a B. subtilis strain with cell shape and growth defects. TIRFM analysis revealed that the mutated MreB protein displayed wild-type localization and dynamics, suggesting that it is still associated to the CW elongation machinery but might be defective in an interaction important for MreB morphogenetic function. Thus, this mutant appears as as a good candidate to start characterizing the interactions between the three MreB isoforms and components involved in CW elongation. It might also help to understand the function of components of theCWsynthetic complexes, and how they are coordinated to achieve efficient CW synthesis. Finally, to investigate how the integrity of the CW is maintained, we studied the localization and dynamics of the LiaIH-system, which i s t he t arget o f L iaRS, a t wo-component system involved in cell envelope stress response. We found that under stress conditions, when liaI and LiaH genes are expressed, the proteins form static complexes that coat the cell membrane. LiaI is required for the even distribution of the LiaH in the membrane. Taken together, these data suggest that LiaIH
complexes may protect the cell from CW damage.
Taken together, the findings described in this thesis provide valuable insights into the
understanding of CW synthesis in B. subtilis, which may open new perspectives for the design of
novel antimicrobial agents
Immobilization of Acetylcholinesterase on Screen-Printed Electrodes. Application to the Determination of Arsenic(III)
Enzymatic amperometric procedures for measuring arsenic, based on the inhibitive action of this metal on acetylcholinesterase enzyme activity, have been developed. Screen-printed carbon electrodes (SPCEs) were used with acetylcholinesterase covalently bonded directly to its surface. The amperometric response of acetylcholinesterase was affected by the presence of arsenic ions, which caused a decrease in the current intensity. The experimental optimum working conditions of pH, substrate concentration and potential applied, were established. Under these conditions, repeatability and reproducibility of biosensors were determined, reaching values below 4% in terms of relative standard deviation. The detection limit obtained for arsenic was 1.1 × 10−8 M for Ach/SPCE biosensor. Analysis of the possible effect of the presence of foreign ions in the solution was performed. The method was applied to determine levels of arsenic in spiked tap water samples.Junta de Castilla y León (BU022A07, Q0968272E) and
the Ministerio de Ciencia e Innovación (TEC2008-01367/TEC) is gratefully acknowledged
Contribution to the kownledge of cultural heritage via a Heritage Information System (HIS): the case of “La Cultura del Agua” in Valverde de Burguillos, Badajoz (Spain)
Modern
science
is
going
through
a
period
of
important
reflection
on
the
role
of
different
agents
and
multiple
disciplines
in
the
management
and
safeguarding
of
architectural
heritage.
This
new
focus
generates
a
greater
amount
and
diversity
of
information,
so
the
implementation
of
a
unifying
tool
in
the
framework
of
digital
information
models
would
mean
a
better
knowledge
of
cultural
heritage
as
well
as
aiding
its
safeguarding
and
protection.
In
addition,
it
must
be
taken
into
account
that,
for
the
correct
management
of
information
in
its
broadest
dimension,
this
tool
must
make
it
possible
to
relate
alphanumeric
data
about
an
item
of
heritage
to
its
spatial
location.
In
this
sense,
this
article
proposes
a
Heritage
Information
System
(HIS)—understood
as
a
digital
knowledge
tool—that
consists
of
a
relational
database
and
a
map
manager
with
Geographic
Information
System
(GIS)
technology
(a
geodatabase).
The
methodology
suggested
here
sets
out
the
steps
that
make
up
the
HIS,
so
that
the
system
can
be
applied
to
other
geographical
elements
or
realities.
For
this
reason,
a
study
was
made
of
“La
Cultura
del
Agua”
in
Valverde
de
Burguillos
(Spain),
a
heritage
ensemble
that
consists
of
rural
architecture
and
dispersed
preindustrial
elements,
which
are
currently
at
risk.
The
HIS
seeks
to
develop
a
more
complete
identification
of
these
elements
(individually
and
as
a
system)
and
a
justified
argument
for
their
being
given
value
and
great
visibility.
This
new
approach
encourages
sustainable
development
in
terms
of
efficiency
and
effectiveness
for
the
analysis,
diagnosis,
and
reactivation
of
cultural
heritage,
always
placing
importance
on
the
balance
of
social
participation
with
the
territory
in
which
the
system
is
applied,
and
with
global
societ
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