Goal conflicts in long-term cropping system trials - the example of carrots

Agricultural research on multiple cropping systems in parallel increases the potential for knowledge transfer between organic and conventional systems. This project aims to develop cropping systems towards greater sustainability through work in long-term trials that have a unique opportunity to contribute to a holistic research perspective. Data on the fourth crop rotation (2007-2012) are now being compiled. This paper presents preliminary results from cultivation of carrots as an example to demonstrate goal conflicts in organic and conventional systems between good nutrient management and good economy on one hand and nematode control and intensive cropping systems (good short-term economy) on the other. Good productivity and sustainable production levels are major overall goals in the project. The conclusion is that more research on nematode susceptibility and propagating at different crops and varieties is very important

3D reflection seismic investigation for mine planning and exploration in the Kevitsa Ni-Cu-PGE deposit, Northern Finland

A 3D reflection seismic survey was conducted over an area of about 9 km2 at the Kevitsa Ni-Cu-PGE (platinum group elements) deposit, Northern Finland. The principal objective of the survey was to image major fault and fracture zones at depth. Understanding the geometry of these zones is important for designing a steep open-pit for mining. Initial processing results suggest that the 3D seismic survey has been successful in imaging both gently dipping and steeply dipping reflections as shallow as 50 ms (or about 150 m), many of which correlate with fault systems and lithological contacts observed at the surface. Several new target areas can be identified in the seismic data that require further investigations for their mineralization potential

Single-nuclei transcriptomes from human adrenal gland reveal distinct cellular identities of low and high-risk neuroblastoma tumors

Childhood neuroblastoma has a remarkable variability in outcome. Age at diagnosis is one of the most important prognostic factors, with children less than 1 year old having favorable outcomes. Here we study single-cell and single-nuclei transcriptomes of neuroblastoma with different clinical risk groups and stages, including healthy adrenal gland. We compare tumor cell populations with embryonic mouse sympatho-adrenal derivatives, and post-natal human adrenal gland. We provide evidence that low and high-risk neuroblastoma have different cell identities, representing two disease entities. Low-risk neuroblastoma presents a tran- scriptome that resembles sympatho- and chromaffin cells, whereas malignant cells enriched in high-risk neuroblastoma resembles a subtype of TRKB+cholinergic progenitor population identified in human post-natal gland. Analyses of these populations reveal different gene expression programs for worst and better survival in correlation with age at diagnosis. Our findings reveal two cellular identities and a composition of human neuroblastoma tumors reflecting clinical heterogeneity and outcome

Partial differential equations for self-organization in cellular and developmental biology

Understanding the mechanisms governing and regulating the emergence of structure and heterogeneity within cellular systems, such as the developing embryo, represents a multiscale challenge typifying current integrative biology research, namely, explaining the macroscale behaviour of a system from microscale dynamics. This review will focus upon modelling how cell-based dynamics orchestrate the emergence of higher level structure. After surveying representative biological examples and the models used to describe them, we will assess how developments at the scale of molecular biology have impacted on current theoretical frameworks, and the new modelling opportunities that are emerging as a result. We shall restrict our survey of mathematical approaches to partial differential equations and the tools required for their analysis. We will discuss the gap between the modelling abstraction and biological reality, the challenges this presents and highlight some open problems in the field

Safety and efficacy of fluticasone propionate in the topical treatment of skin diseases

Fluticasone propionate - the first carbothioate corticosteroid - has been classified as a potent anti-inflammatory drug for dermatological use. It is available as 0.05% cream and 0.005% ointment formulations for the acute and maintenance treatment of patients with dermatological disorders such as atopic dermatitis, psoriasis and vitiligo. This glucocorticoid is characterized by high lipophilicity, high glucocorticoid receptor binding and activation, and a rapid metabolic turnover in skin. Although skin blanching following fluticasone propionate exceeds that of corticosteroids of medium strength, several clinical trials demonstrate a low potential for cutaneous and systemic side-effects, even in difficult-to-treat areas like the face, the eyelids and intertriginous areas. Even among paediatric patients with atopic dermatitis, fluticasone propionate proved to be safe and effective. These pharmacological and clinical properties are reflected by the high therapeutic index of this glucocorticoid

Role of fatty acid transporters in epidermis: Implications for health and disease

Skin epidermis is an active site of lipid synthesis. The intercellular lipids of human stratum corneum (SC) are unique in composition and quite different from the lipids found in most biological membranes. The three major lipids in the SC are free fatty acids, cholesterol and ceramides. Fatty acids can be synthesized by keratinocytes de novo and, in addition, need to be taken up from the circulation. The latter process has been shown to be protein mediated, and several fatty acid transporters are expressed in skin. Recent studies of transgenic and knockout animal models for fatty acid transporters and the identification of fatty acid transport protein 4 (FATP4 or SLC27A4) mutations as causative for Ichthyosis Prematurity Syndrome highlight the vital roles of fatty acid transport and metabolism in skin homeostasis. This review provides an overview of our current understanding of the role of fatty acids and their transporters in cutaneous biology, including their involvement in epidermal barrier generation and skin inflammation

Preface: Interdisciplinary contributions from the Division on Energy, Resources and the Environment at the EGU General Assembly 2019

Since 2004, the European Geosciences Union (EGU) brings together experts from all over the world at its annual General Assembly, covering all disciplines of the earth, planetary and space sciences. With this special issue in Advances in Geosciences, we are pleased to present a collection of contributions from the Division on Energy, Resources and the Environment (ERE) which were presented at the EGU General Assembly 2019 in Vienna

Design of a Microsphere-Based High-Throughput Gene Expression Assay to Determine Estrogenic Potential

Recently gene expression studies have been multiplied at an accelerated rate by the use of high-density microarrays. By assaying thousands of transcripts at a time, microarrays have led to the discovery of dozens of genes involved in particular biochemical processes, for example, the response of a tissue/organ to a given chemical with therapeutic or toxic properties. The next step in these studies is to focus on the response of a subset of relevant genes to verify or refine potential therapeutic or toxic properties. We have developed a sensitive, high-throughput gene expression assay for this purpose. In this assay, based on the Luminex xMAP system, carefully selected oligonucleotides were covalently linked to fluorescently coded microspheres that are hybridized to biotinylated cRNA followed by amplification of the signal, which results in a rapid, sensitive, multiplexed assay platform. Using this system, we have developed an RNA expression profiling assay specific for 17 estrogen-responsive transcripts and three controls. This assay can evaluate up to 100 distinct analytes simultaneously in a single sample, in a 96-well plate format. This system has improved sensitivity versus existing microsphere-based assays and has sensitivity and precision comparable with or better than microarray technology. We have achieved detection levels down to 1 amol, detecting rare messages in complex cRNA samples, using as little as 2.5 μg starting cRNA. This assay offers increased throughput with decreased costs compared with existing microarray technologies, with the trade-off being in the total number of transcripts that can be analyzed

Shifting patterns of genomic variation in the somatic evolution of papillary thyroid carcinoma

Shows Single Nucleotide Substitutions in the MAPK Pathway. (XLSX 43 kb

The effect of organic retarders on grout thickening and setting during deep borehole disposal of high-level radioactive waste

Deep borehole disposal (DBD) is being increasingly seen as a viable and potentially superior alternative to comparatively shallow mined repository concepts for disposal of some high-level radioactive wastes. We report here details of proof-of-concept investigations into the use of cementitious grouts as sealing/ support matrices for use in low temperature DBD scenarios. Using the cementitious grout to fill annular space within the disposal zone will not only support waste packages during placement, but will also provide a low permeability layer around them which will ultimately enhance the safety case for DBD. Grouts based on Class G oil well cement are being developed. The use of retarders to delay the accelerated onset of thickening and setting (caused by the high temperature and pressure in the borehole) is being investigated experimentally. Sodium gluconate and a polycarboxylate additive each provide suf- ficient retardation over the range 90e140 °C in order to be considered for this application. Phosphonate and sulphonate additives provide desirable retardation at 90 °C. The additives did not affect grout composition at 14 days curing and the phases formed are durable at elevated temperature and pressure